Colin.Farquharson at bbsrc.ac.uk
Tue Jan 28 10:49:26 EST 1997
I am trying to separate chondrocytes into their different sizes using
Percoll (Pharmacia) discontinuous gradients (1.04 - 1.08). 2mls of
ech are gently layered on top of each other and the cells suspended
(20-40 million) in 1 ml of medium on top. Spun in a swing out motor
at 400g for 30 min at RT. Things often works but often there is
extensive clumping at the 1.06 band and this results in poor
separation. Cells appear to be 'dragged down' from their band above
and cells that should move further down are inhibited from doing so.
Has anyone else had trouble with cells clumping together and anyone
know how to resolve it ?
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