Sick MCF-7: HELP!

Gerald Denis gdenis at BU.EDU
Wed Sep 17 10:21:35 EST 1997


I am carrying MCF-7 cells for the first time (purchased from ATCC and
frozen after 1 passage).  I thawed them out into DMEM supplemented with
L-glutamine and 10% fetal calf serum.  They are growing very slowly and
do not look healthy.  Is there a better media to use?  Should I be
adding additional supplements?
Please respond to my e-mail address: karynvan at bu.edu
Thanks in advance!



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