Freezing human keratinocytes
tiwamoto at leo.bekkoame.or.jp
Sat Feb 14 07:32:53 EST 1998
> I want to freeze human keratinocytes
> but I have no technical methods
Do you want to use the frozen keratinocytes for cell culture? Or
staining such as freeeze-fracture for EM or monoclonal antibody?
I usually throw cells suspension in MEM (such as DMEM or EMEM) with 10%
FCS and 10% DMSO into liquid nitrogen.
Otherwise more easy ,insted of MEM+FCS+DMSO you can purchase a liquid
medium specilized for cell freeze. In Japan it is sold as "Cell Banker".
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