Mycoplasma infection curing ?

[Incheol Shin]

Dear Researchers,

Lately we are in a severe difficulty in cell-line culture
due to mycoplasma infection. Lots of workers share
one culture room, moreover one of them is doing primary cell 
culture from rat hippocampus. Despite of curing the mycoplasma-
infected cells using BM-cyclin (BM Co.), reinfection usually 
occurred in a few months. Now, we want to know the source of 
mycoplasma and how it can be prevented.
As I know, primary cell culture or tissue is major source of 
mycoplasma infection. Is it really true ?   

Thanks in advance for any of your kind help

Incheol Shin, Ph.D.
Dept of Biochemistry
College of Medicine, Hanyang Univ.
Seoul, Korea