From francis_kuttner at hotmail.com Sat May 1 18:00:24 1999 From: francis_kuttner at hotmail.com (francis_kuttner@hotmail.com) Date: Mon Mar 7 05:41:10 2005 Subject: radiobiology Message-ID: <7gg128$l6b$1@nnrp1.dejanews.com> dear people, i am a graduate student at tufts university and i am very much interested in radiobiology and radition chemistry. currently i am looking for a research laboratory that would be interested to accomodate me and sponsor my research. i have narrowed my focus in radiobiology to apoptosis in mouse lymphocytes. and i am wondering if any of you would comment on the possible research questions that i have come across. please email me at francis_kuttner@hotmail.com if you have any ideas concerning my research topics or forward this post to anyone you know who is interested in hiring! thanks in advance!! 1. normal mouse lymphocytes (thymocytes) show that normally, under unstressed conditions, a large fraction of the total lymphocyte population can be identified as apoptotic cells. 2. cells showing large extents of apoptosis that are generally more radiosensitive than cells showing smaller extents of apoptosis. 3. as dosage increases, the increase in percentage of apoptotic cells is essentially the same in the neutron or photon irradiated cells. 4. there clear evidence of a G2 block after the 3 Gray doses. 5. it cannot be determined if there is a G1 block because the decrease in the height of the G1 peak may simply reflect cells becoing apoptotic rather than progressing into S phase. 6. there is clear evidence of a large Q cell population in the control cells. 7. the km for the dose response curves is about 45 rad for both curves. ~francis -----------== Posted via Deja News, The Discussion Network ==---------- http://www.dejanews.com/ Search, Read, Discuss, or Start Your Own From ssbaumgart at foni.net Sun May 2 01:51:57 1999 From: ssbaumgart at foni.net (S. Baumgart) Date: Mon Mar 7 05:41:10 2005 Subject: split ratio Message-ID: <7ggsog$m9u$1@renate.komtel.net> Hi! What does the "split ratio" means refering to cultured neoplastic cells? From stockinvest234 at YAHOO.COM Sun May 2 03:59:32 1999 From: stockinvest234 at YAHOO.COM (stockinvest234@YAHOO.COM) Date: Mon Mar 7 05:41:10 2005 Subject: Total Profit +980% in 1998!!! Message-ID: <199905020840.SAA17217@orange.one.net.au> ================================== Our 1998 Stock Picks - Total Profit +980%!!! ================================== Subscribe to our Newsletter to be Informed of our Stock Picks - 100% FREE!!! VALUE stocks with outstanding company news AND upcoming extensive promotion are virtually GUARANTEED profits - you only need an early stock purchase to profit. Our total profit for 1998 was +980%!!! Subscribe to our FREE newsletter, and we'll notify you of stocks we've selected - this is where we put OUR money! 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From arthurr at wsunix.wsu.edu Mon May 3 02:02:40 1999 From: arthurr at wsunix.wsu.edu (art roberts) Date: Mon Mar 7 05:41:11 2005 Subject: new website for microbiologists Message-ID: <372D4A0F.B2FAD600@wsunix.wsu.edu> http://biotech.isCool.net This site allows easy access to hyperlinks and free software for the biochemist, biophysicist and molecular biologist. This site is constantly evolving and expanding, so it can be easy to use, reliable, and comprehensive. This site is non-profit and for your ease and enjoyment. Sincerely, Art Roberts (web designer) From bogus@does.not.exist.com Mon May 3 06:31:02 1999 From: bogus@does.not.exist.com () Date: Mon Mar 7 05:41:11 2005 Subject: Pteridophytes newsgroup Message-ID: <7gk1dm$hg5$1@netserv.univ-lille1.fr> Du fait de leur histoire, les cryptogames vasculaires ont conserve des traits relativement archaiques et des particularites biologiques qui les distinguent des autres cormophytes. Ce groupe de vegetaux merite, de ce fait, une attention pariculiere de la part des biologistes. l?experience demontre, en effet, que l?analyse de phenomenes dans des groupes consideres comme "peu ?volu?s" apporte souvent des ?l?ments de compr?hension d?terminants.Si certains domaines de la Biologie ou de la Physiologie des Pt?ridophytes font actuellement l'objet d'?tudes, d'autres vraisemblablement plus nombreux sont laiss?s dans l'ombre. Si vous ?tes int?ress?s par la mise en place d'un r?seau de chercheurs travaillant ou m?me simplement r?fl?chissant sur la biologie de ces plantes, ce qui permettrait un ?change et une concertation pour envisager l'avenir de l'?tude de ce groupe en France, manifestez-vous par messages ?lectroniques de pr?f?rence. - Mr H.ESPAGNAC : Morphogen?se, Tel. 00 33/ (0)4 90 14 44 51, Fax: 00 33/ (0)4 90 14 44 09, E-mail:henri.espagnac@univ-avignon.fr -Mme N.FERRIERE : Histologie, Tel. 00 33/ (0)4 67 61 58 30, Fax: 00 33/ (0)4 67 61 56 05, E-mail :ferriere@cirad.fr -Mr J.GALTIER: Pt?ridophytes fossiles, Tel. 00 33/ (0)4 67 14 36 31, E-mail: galtier@isem.univ-mpt2.fr From hbdi at idt.net Mon May 3 07:27:43 1999 From: hbdi at idt.net (NDRI/HBDI) Date: Mon Mar 7 05:41:11 2005 Subject: Human Tissues, Organs and DNA from Families for Medical Research Message-ID: <7gk4of$6m9@nnrp2.farm.idt.net> See www.ndri.com for access to human tissues and organs for medical researchers See www.hbdi.org for information about family collections containing DNA and immortalized cells - over 600 families - for medical and genetic researchers NDRI and HBDI are non-profit organizations aiding medical research and the search for disease genes. From youkim at HANMAIL.NET Mon May 3 09:34:15 1999 From: youkim at HANMAIL.NET (Yong-Ou Kim) Date: Mon Mar 7 05:41:11 2005 Subject: About p53 in yeast Message-ID: <19990503230947.HM.00000000000MQbQ@hanmail.net> Hi everyone, I have a question. I think p53 is a master transacting factor regulating cell proliferation (and cell cycle). Is there p53-like protein in yeast? I can't find that in database. If not, how does yeast respond to DNA damage that activate p53 in mammalian cells? ================================================== 평생 쓰는 무료 E-mail 주소 한메일넷 http://www.hanmail.net From classiest at CTIMAIL.COM Mon May 3 23:43:28 1999 From: classiest at CTIMAIL.COM (Classiest Ins.) Date: Mon Mar 7 05:41:11 2005 Subject: “㈈ゐ곌ㅊ짩쩕싻�`텻“ Message-ID: <419.436284.52489062classiest@ctimail.com> 옹톀ずトㅱ좬 �{ㅅ퇰좦답츙첷켎ㅑ좥퇱�`㈈ゐⓥ쫆쨢┬죌뚫좥쫇곌ㅊ 쨠�@⒝�획C좥퇥�@텻�~㉵촅탊Ν좥빅ユ촇귄촑②ㄳ췦퐓좥О죽쬗촇귄 ㈄�~ �빱쉈q죣뮴멍첦픇휱격┳��ㅍ쩻죥쩘쾩ㄷ죣�뺨芯제w첦 픇┳��ㅍ쩻죥�{퇱펣찯з시Ν�U칂�\�{쩳좥뇩ⓔ뱉톝죣텻�~ユ귄쭤 쟨죥첦픇춑뭔좥쩙콄쫤ㅯ좧 첦쁑폻놋좬 뇩ⓔ�@�~┳�칟㎎N�~ユ귄쭤쟨첦촤�鐸AΑ쫤첦픇춗쿭ㅴ쩒쫇펣찯з좦 붑ヘз폻놋ㅊ�]텻�~앉쵵ㄷ쭤쟨턔Ξ좧㏓첦짩켔쩳쟌탊�@켲죣텻�~ユ 귄쭤쟨첦픇쩮죥�@Оㅹメユ귄쭤쟨첦촤�態慊納Aユ귄녔�已K�|�@쩧쬨 ��촇귄좧┨죣첦픇쩮죥퐸Ξ⒀⒡┳펣찯з좦붑ヘз시Ν�U붸죠짾ㄷ쭤 �|⒝쭤쟨녔�議C 첦픇�胎B좬쭯쯸텻�~㉵촅넋값쩳툲↗↘줓¤¿좥¿¿¿좧 첦픇�~턔좬↗↘줓∑¤¿좧 촥멕먹괌좥싻쵵퉞 뮴멍첦픇휱격┳��ㅍ쩻∮∑∑∮ ‰◁¿◁ **�뼘깽떰홈璣機衲㎘科A┳츙깡닥랫쩑첦녔О렷좧** _ 쫜쩑쨁멸�틜癤邏Uㅮ쵵ㄳ첢좥�빱쉈q쫠┨쵵틄좥멸�튈u탊캿�@┯좧 From rcjohnsen at aol.com Tue May 4 07:31:11 1999 From: rcjohnsen at aol.com (Rcjohnsen) Date: Mon Mar 7 05:41:11 2005 Subject: “�1챒곌ㅊ짩쩕 D�`텻“ References: <419.436284.52489062classiest@ctimail.com> Message-ID: <19990504083111.16137.00001814@ng-fa1.aol.com> << Subject: ???1?d?????H?K D?`?N?? From: classiest@CTIMAIL.COM ("Classiest Ins.") Date: Tue, May 4, 1999 4:43 AM Message-id: <419.436284.52489062classiest@ctimail.com> ???R???????G >> Why don't you just get off till you learn communication skills? From francis_kuttner at hotmail.com Tue May 4 11:49:20 1999 From: francis_kuttner at hotmail.com (francis_kuttner@hotmail.com) Date: Mon Mar 7 05:41:11 2005 Subject: Cell Population Kinetics Message-ID: <7gn8ef$ghu$1@nnrp1.dejanews.com> Hi, I posted a message a few days ago concerning my potential research topics in radiobiology and radiation chemistry, and I have heard from a few people. Thanks! I am currently brushing up on my knowledge of cell population kinetics but in this review I am encountering some trouble. If anyone can help please write me at francis_kuttner@hotmail.com. Thank you. ~Francis 1. Why are oxic cells more radiosensitive to apoptosis than anoxic cells? 2. Why do radiosensitizing effects of oxygen disappear at high doses of radiation? 3. Deletion of the Rb gene in myc. Decreases survival? Increases apoptosis? 4. Deletion of the cyclin D gene in ras cells. Decreases survival? Decreases apoptosis? 5. Overexpression of the cyclin B gene in ras and myc cells. Same survival as in controls? Increases apoptosis? 6. From the viewpoint of effects on apoptosis, a decrease in the cellular supply of ATP should decrease cell survival? 7. Why does the repair saturation model as presented mathematically and experimentally fail as a comprehensive model of cell killing? -----------== Posted via Deja News, The Discussion Network ==---------- http://www.dejanews.com/ Search, Read, Discuss, or Start Your Own From francis_kuttner at hotmail.com Tue May 4 12:44:13 1999 From: francis_kuttner at hotmail.com (francis_kuttner@hotmail.com) Date: Mon Mar 7 05:41:11 2005 Subject: Cell Population Kinetics Message-ID: <7gnblc$joo$1@nnrp1.dejanews.com> Hi, I posted a message a few days ago concerning my potential research topics in radiobiology and radiation chemistry, and I have heard from a few people. Thanks! I am currently brushing up on my knowledge of cell population kinetics but in this review I am encountering some trouble. If anyone can help please write me at francis_kuttner@hotmail.com. Thank you. ~Francis 1. Why are oxic cells more radiosensitive to apoptosis than anoxic cells? 2. Why do radiosensitizing effects of oxygen disappear at high doses of radiation? 3. Deletion of the Rb gene in myc. Decreases survival? Increases apoptosis? 4. Deletion of the cyclin D gene in ras cells. Decreases survival? Decreases apoptosis? 5. Overexpression of the cyclin B gene in ras and myc cells. Same survival as in controls? Increases apoptosis? 6. From the viewpoint of effects on apoptosis, a decrease in the cellular supply of ATP should decrease cell survival? 7. Why does the repair saturation model as presented mathematically and experimentally fail as a comprehensive model of cell killing? -----------== Posted via Deja News, The Discussion Network ==---------- http://www.dejanews.com/ Search, Read, Discuss, or Start Your Own From francis_kuttner at hotmail.com Tue May 4 12:44:26 1999 From: francis_kuttner at hotmail.com (francis_kuttner@hotmail.com) Date: Mon Mar 7 05:41:11 2005 Subject: Cell Population Kinetics Message-ID: <7gnblp$jor$1@nnrp1.dejanews.com> Hi, I posted a message a few days ago concerning my potential research topics in radiobiology and radiation chemistry, and I have heard from a few people. Thanks! I am currently brushing up on my knowledge of cell population kinetics but in this review I am encountering some trouble. If anyone can help please write me at francis_kuttner@hotmail.com. Thank you. ~Francis 1. Why are oxic cells more radiosensitive to apoptosis than anoxic cells? 2. Why do radiosensitizing effects of oxygen disappear at high doses of radiation? 3. Deletion of the Rb gene in myc. Decreases survival? Increases apoptosis? 4. Deletion of the cyclin D gene in ras cells. Decreases survival? Decreases apoptosis? 5. Overexpression of the cyclin B gene in ras and myc cells. Same survival as in controls? Increases apoptosis? 6. From the viewpoint of effects on apoptosis, a decrease in the cellular supply of ATP should decrease cell survival? 7. Why does the repair saturation model as presented mathematically and experimentally fail as a comprehensive model of cell killing? -----------== Posted via Deja News, The Discussion Network ==---------- http://www.dejanews.com/ Search, Read, Discuss, or Start Your Own From GysdeJongh at csi.com Tue May 4 15:39:02 1999 From: GysdeJongh at csi.com (Gys de Jongh) Date: Mon Mar 7 05:41:11 2005 Subject: split ratio References: <7ggsog$m9u$1@renate.komtel.net> Message-ID: <#5IE13ml#GA.346@ntawwabp.compuserve.com> The spit ratio = the area available to the cells after passage / the area the cells were harvested from. So if you have 1 dish of cultured neoplastic cells , trypsinize and reseed all of them in 5 new dishes of the same size then the split ratio = 5 Enjoy your research Gys de jongh S. Baumgart wrote in message <7ggsog$m9u$1@renate.komtel.net>... >Hi! > >What does the "split ratio" means refering to cultured neoplastic cells? > > From mdonken at artsci.wustl.edu Tue May 4 15:56:16 1999 From: mdonken at artsci.wustl.edu (Michael Onken) Date: Mon Mar 7 05:41:11 2005 Subject: split ratio References: <7ggsog$m9u$1@renate.komtel.net> Message-ID: <372F5EEE.4D3E6B06@artsci.wustl.edu> "S. Baumgart" wrote: > Hi! > > What does the "split ratio" means refering to cultured neoplastic cells? I would assume this refers to the extent to which the cells should be diluted during each passage. For example, a split ratio of 1:3 would mean that the cells from a near confluent plate should be passed to new plates such that a plate of equal size to the original would get one third of the cells. Hope this clarified rather than further confused, Mike Onken -- Michael Onken -. .-. .-. .-. . mdonken@artsci.wustl.edu Mol. Cell Biol. ||X|||\ /|||X|||\ /| URL: Washington Univ |/ \|||X|||/ \|||X|| http://madsci.wustl.edu/ St. Louis, MO ' `-' `-' `-' `- From Lisa.L.Huang at usa.dupont.com Wed May 5 02:24:34 1999 From: Lisa.L.Huang at usa.dupont.com (Lisa L Huang) Date: Mon Mar 7 05:41:11 2005 Subject: Microbial Molecular Biologist MS level positions Message-ID: <85256767.00784153.00@CDCLN24.LVS.DUPONT.COM> Microbial Molecular Biologist MS level positions (Two) - DuPont Central Research & Development Two positions are currently available for microbial molecular biologists to join an interactive group in DuPont Central Research & Development (Wilmington, Delaware). Interest in cellular function is a prerequisite for these appointments. The individual should have knowledge of microbial genetics, microbial physiology and biochemistry with an emphasis on biological regulatory mechanisms including feedback inhibition, covalent protein modification and transcriptional regulatory mechanisms such as repression and attenuation. It is anticipated that the successful individual will perform a variety of molecular biological techniques including recombinant DNA methodology, strain construction, small molecule extraction/measurement and RNA extraction/measurement. Gene expression profiling using DNA arrays and gene fusions will be a focus. He/She will be using various bioinformatics tools for data aquisition and analysis. REQUISITE SKILLS/EXPERIENCE: - Master's degree in biological science or equivalent on-the-job experience - Willingness to work with radioisotopes - Laboratory experience in: Recombinant DNA technology, microbiological culture techniques, microbial genetics & biochemistry. - Computer skills, including word processing, spreadsheets, database, graphics and the Internet in both MAC and PC environments - Desire to present research directions/results in a variety of forums CONTACT: If interested, please email your resume to Lisa Huang (Lisa.L.Huang@usa.dupont.com) and Bob LaRossa (Robert.A.LaRossa@usa.dupont.com). Please also send a copy to Carol Wilde (Carol.J.Wilde_CPS@usa.dupont.com). Lisa Huang DuPont Experimental Station P.O. Box 80173 E173/203 Wilmington, DE, 19880-0173. Bob LaRossa DuPont Experimental Station P.O. Box 80173 E173/216 Wilmington, DE, 19880-0173. Recent Publications from the LaRossa lab: LaRossa, R. A. (1999) ?Nutritional Mutations? in Encyclopedia of Genetics, S. Brenner and J. Miller, Eds., Academic Press, New York LaRossa, R. A., editor (1998) Bioluminescent Methods and Protocols. Humana Press. Epelbaum, S., LaRossa, R. A., Van Dyk, T. K., Chipman, D. M. (1998) Branched chain amino acid synthesis in Salmonella typhimurium: a quantitative analysis. J. Bacteriol. 180: 4056-4067. Van Dyk, T. K., Ayers, B. L., Morgan, R. W. and LaRossa, R. A. (1998) Constricted flux through the branche-chain amino acid biosynthetic enzyme acetolactate synthase triggers elevated expression of genes reulated by rpoS and internal acidification. J. Bacteriol. 180: 785-792. Vollmer, A. C., Belkin, S., Smulski, D. R, Van Dyk, T. K. and LaRossa, R. A. 1997. Detection of DNA damage by use of Escherichia coli carrying recA'::lux, uvrA'::lux or alkA'::lux reporter plasmids. Applied and Environ. Microbiol. 63: 2566-2571. Belkin, S, Smulski, D. R., Dadon, S., Vollmer,A. C., Van Dyk, T. K. and LaRossa, R. A. 1997. A panel of stress-responsive luminous bacteria for toxicity detection. Water Research 31: 3009-3016. Belkin, S., D. R. Smulski, A. C. Vollmer, T. K. Van Dyk and R. A. LaRossa. 1996. Oxidative stress detection using Escherichia coli bearing a katG'::lux fusion. Applied and Environ. Microbiol. 62: 2252-2256. Representative Publications of Lisa Huang: Foster, P., Huang, L., Santi, D. V. and Stroud, R. ?The Structural Basis for Pseudouridine Formation in tRNA: Pseudouridine Synthase I at 1.5 ? Resolution,? submitted to Science. Huang L, Ku J, Pookanjanatavip M, Gu X, Wang D, Greene PJ, Santi DV. "Identification of two Escherichia coli pseudouridine synthases that show multisite specificity for 23S RNA," Biochemistry, 1998, 37, 15951-7. Huang L., Pookanjanatavip, M., Gu, X. and Santi, D. V. "A Conserved Aspartate of tRNA Pseudouridine Synthase is Essential for Activity and a Probable Nucleophilic Catalyst," Biochemistry, 1998, 37,344-351. Huang L., Sera, T. and Schultz, P. G. "A Permutational Approach toward Protein-DNA Recognition," Proceedings of the National Academy of Sciences, USA, 1994, 91, 3969-3973. From f.muller at adilinstruments.com Wed May 5 06:57:44 1999 From: f.muller at adilinstruments.com (Franck Muller) Date: Mon Mar 7 05:41:11 2005 Subject: <<<< Adil Instruments - The microplate washer expert >>>> Message-ID: <925905450.984843@casablanca.magic.fr> Adil Instruments is specialised in developing and manufacturing laboratory instruments. With its 17 years of experience and innovative top-quality products, Adil Instruments can now offer a complete range of microplate washers compatible with 96 and 384 well formats. For more details about our products visit our site at http://www.adilinstruments.com/ **************************************************************************** ** World leader for microplate washers since 1985. ** Worldwide OEM distribution ** A Complete range of microplate washers : - Easy strip washer LP21 - Fast and efficient LP40/LP41 series - High Throughput System full plate washer LP440 ** 96- and 384-well plate compatibility of the whole LP4X and LP440 washers. **************************************************************************** From 915608 at candseek.com Wed May 5 10:50:40 1999 From: 915608 at candseek.com (915608@candseek.com) Date: Mon Mar 7 05:41:11 2005 Subject: JOBOP RES SCIENTIST IMMUNOLOGY Message-ID: <199905051550.IAA00121@net.bio.net> Since your email address was listed on a related web site page or database, I thought you might help. I am seeking an individual within the following conditions: A research scientist who will work for a biotech company that is developing novel therapeutic products including interventional therapeutics, cancer vaccines and blood cell growth factors for the treatment of cancer and cancer- related disorders. Should have a background in immunology, tumor immunology and cancer biology. This person will be required to do some animal handling which would include developing new agents, cell biology, and have the ability to plan and prioritize work independently to completion. Experience in endothelial cell biology, angiogenesis, and flow cytometry is a plus. Should possess a B.S.,M.S.or MD degree in the biological sciences. Our client is a leading biotech firm with research facilities in New York and can provide excellent benefits (health insurance, dental, and vision plan, paid vacation and more). A high impact, high profile position with excellent opportunity for advancement. Geographic Location of Position: US-NY If you know anyone that might be interested, please forward this to them or contact: Stan Sundstrom DMC Voice: 609-584-8733 Ext. 218 Fax: 609-584-9575 Email: 915608@candseek.com To permanently discontinue receiving employment opportunity notices from any and all help wanted advertisers using the Candidate Seeker system, click your "Reply" button and type the word "re- move" without spaces between the letters into the SUBJECT field then click the "Send" button. Your email address will be permanently filtered from ALL future job opportunity notifications sent via the Candidate Seeker system. To temporarily filter employment opportunity notices sent via the Candidate Seeker system, type the acronym "JOBOP" into your subject filter. All employment opportunity notices sent via the Candidate Seeker system contain the acronym "JOBOP" in the subject so they may be easily filtered or blocked if so desired. Other email addresses may be permanently deleted from future contact by emailing a single blank message from the desired address to nomail@candseek.com. Enter additional addresses into the body of the message and they will also be added to the "nomail" list Please feel free to contact the candidateseeker.com feedback line at 609-584-5499. Do not use this number for job related questions. All job related questions should be directed to the employer by replying to contact addresses or phone numbers indicated at the end of the job description message. From francis_kuttner at hotmail.com Thu May 6 15:53:14 1999 From: francis_kuttner at hotmail.com (francis_kuttner@hotmail.com) Date: Mon Mar 7 05:41:11 2005 Subject: Repair Saturation Model Message-ID: <7gsvfp$juu$1@nnrp1.deja.com> Why does the repair saturation model as presented mathematically and experimentally fail as a comprehensive model of cell killing? Is this because the repair saturation model does not include concepts of biomedical repair, i.e. only hits to targets and accumulation of subleathal damange where all targets are hit whence death ensues? ~Francis -----------== Posted via Deja News, The Discussion Network ==---------- http://www.dejanews.com/ Search, Read, Discuss, or Start Your Own From francis_kuttner at hotmail.com Thu May 6 15:49:10 1999 From: francis_kuttner at hotmail.com (francis_kuttner@hotmail.com) Date: Mon Mar 7 05:41:11 2005 Subject: Repair Saturation Model Message-ID: <7gsv86$jis$1@nnrp1.deja.com> Why does the repair saturation model as presented mathematically and experimentally fail as a comprehensive model of cell killing? Is this because the repair saturation model does not include concepts of biomedical repair, i.e. only hits to targets and accumulation of subleathal damange where all targets are hit whence death ensues? ~Francis -----------== Posted via Deja News, The Discussion Network ==---------- http://www.dejanews.com/ Search, Read, Discuss, or Start Your Own From successbiz at EMAILER.COM Thu May 6 17:46:18 1999 From: successbiz at EMAILER.COM (successbiz@EMAILER.COM) Date: Mon Mar 7 05:41:12 2005 Subject: How To Increse Your Sales By Using Powerful Online Tool!![AD] Message-ID: <156564594656.GPP09661215@nowhere.com> This mailings are sent complying to the proposed United States Federal requirements for commercial e-mail: Section 301 Paragraph (a)(2)(C) of S. 618. Please see the bottom of this message for further information and removal instructions. ************************************* Dear Marketer, Do you have products/business that you would like to promote online? Do you want to do it cheap, yet affective way? Would you like to be able to contact new prospects by Email about your existing products, services, or opportunities If you have products or business that you'd like to promote using powerful online marketing tools, please email to Moreinfobox@yahoo.com and put "More Info." as your subject. Thank you! ==================================== Per Section 301, Paragraph (a)(2)(C) of S. 618, further transmissions to you by the sender may be stopped at NO COST to you by forwarding this e-mail to recyclebin@angelfire.com From m.winter at auckland.ac.nz Fri May 7 01:52:01 1999 From: m.winter at auckland.ac.nz (Markus Winter) Date: Mon Mar 7 05:41:12 2005 Subject: Poison sequences? Message-ID: <37328D90.D47219EB@auckland.ac.nz> Hi asll, does anyone have information about "Poison sequences" in vectors/plasmids? I'm having a hard time getting any plasmid DNA out of a vector with insert after transformation into E. coli DH5-alpha, while I can get masses of plasmid DNA with the vector alone. The insert should not be expressed since it is under the control of a "mammalian" promoter (BPV). Any help is greatly appreciated. Markus m.winter@auckland.ac.nz P.S. Please answer by e-mail since I only infrequently read the newsgroup. From mveltmaat at hotmail.com Fri May 7 06:31:42 1999 From: mveltmaat at hotmail.com (Martijn Veltmaat) Date: Mon Mar 7 05:41:12 2005 Subject: signal transduction Message-ID: <3732CF1E.8F3CC2AE@hotmail.com> Is there anybody outthere, who has a nice compilation scheme of all the molecules and (faulty) signal transduction processes that go on in cancer? I know it's a lot to ask for, but I hate to waste my time preparing such a scheme, while it's already available. From bizop3 at USWEST.NET Fri May 7 07:23:02 1999 From: bizop3 at USWEST.NET (Gary Phillips) Date: Mon Mar 7 05:41:12 2005 Subject: Question Message-ID: <199905071223.FAA22173@net.bio.net> I received your email address from a list of opportunity seekers I purchased. I just wanted to double check to make sure it was ok for me to send my opportunity to you. If you would not like to receive my message just mailto:bizop5@hotmail.com with "Remove" on the Subject Line and I will remove your name from my list Thanks, Gary Phillips ------------------------------------------ This message was sent to you by Name: Gary Phillips Email Address: bizop3@uswest.net IP Address: xdialup166.phnx.uswest.net ------------------------------------------ Using Aureate Group Mail Free Edition Find out more about this product and try it for free at: http://www.group-mail.com/1 From office at selectscience.net Fri May 7 10:06:22 1999 From: office at selectscience.net (SelectScience) Date: Mon Mar 7 05:41:12 2005 Subject: This Week's Chemistry & Life Science Industry News Headlines Message-ID: <7gusum$bur$4@news1.cableinet.co.uk> This week, www.selectscience.net reported on 37 news stories the day they happened, so if you need to keep up to speed on the latest business developments in chemistry and life science, make the news ticker on www.selectscience.net part of your daily routine. Andrew Smith (andrew@selectscience.net) Editor, www.selectscience.net This week's headlines were: DNA Chip Technology Enhances Consumer Safety and Water Quality Celera Genomics Commences Sequencing the Drosophila Melanogaster Genome Glaxo Wellcome, Emory University & Triangle Pharmaceuticals Resolve Coviracil Dispute Advanced Tissue Sciences Presents Data on Vascular Endothelialization Growth Factor (VEGF) Secretion CYTOGEN Reports First Profitable Quarter and Announces Strategic Emphasis on Prostate Disease Ribozyme Pharmaceuticals, Inc. Reports First Quarter Financial Results UCSB Researchers Disarm ""Trojan Horse"" Pathogens" Incyte Score Latest Victory in Patent Battle PE Biosystems Partners With Verdot Industries Bio-Rad Laboratories and Saigene Corporation enter into a worldwide exclusive licensing agreement Bio-Rad Reports Results For First Quarter 1999 Thermedics Detection Announces First Quarter Results Study examined safety of HFA-134a BDP compared to traditional Becolmethasone inhalers La Jolla Pharmaceutical Company Reports First Quarter 1999 Financial Results Genset and Johns Hopkins Sign Schizophernia Gene Agreement Form of Chinese Herb Found to Temper Immune System and Kill Cancer Cells Astra Files Suit Against German Generic Company What Changes is HCFA Proposing to the Medicare Coverage Process Nycomed Pharma Sold to Nordic Capital Progenics Pharmaceuticals, Inc. Reports First Quarter 1999 Results Thermo Electron To Buy Its Own And Its Subsidiaries' Securities SIBIA Neurosciences, Inc. Names Carla M. Suto, Ph.D. Vice President, HTS and Technology Development HemaSure Inc. Completes $9 Million Private Placement COBE Laboratories Trega Biosciences Reports First-Quarter Results; Revenues Increase 75% BD Labware & Kimble/Kontes Form Collaborative Agreement Roslin Institute & Geron Sign Major Deal to Advance Dolly Technology Affymetrix Reports First Quarter Results Dade Behring Announces Hoechst AG Will Increase Ownership Position QIAGEN and EVOTEC Form Joint Venture for the Development and Commercialization of High-Throughput Systems HemaSure Inc. and COBE BCT Inc. Initiate Patent Lawsuit With Pall Corporation EG&G/PE Analytical Instruments Deal on Schedule; US & German Rebiew Periods Expire New Genetic Study Points Way For Vaccine Reaction Research QIAGEN Up 33% in First Quarter Financial Results Inspire Awarded U.S. Key Patent for New Chemical Entities Cadus Pharmaceutial Corporation to Explore Strategic Alternatives Glyko, Inc. Acquires Biochemical Reagent Business from Oxford GlycoSciences Plc Sanofi Reports 6.8% Sales Growth in the First Quarter of 1999 (8.6 % for Pharmaceuticals) From hbdi at idt.net Fri May 7 14:58:46 1999 From: hbdi at idt.net (NDRI/HBDI) Date: Mon Mar 7 05:41:12 2005 Subject: Autopsy, Surgical Discard and Transplantation Tissues for Now Available for Research Message-ID: <7gvguj$b9a@nnrp2.farm.idt.net> See www.ndri.com for access to human tissues and organs for medical researchers See www.hbdi.org for information about family collections containing DNA and immortalized cells - over 600 families - for medical and genetic researchers NDRI and HBDI are non-profit organizations aiding medical research and the search for disease genes. From drgonfly at ultranet.com Fri May 7 15:18:29 1999 From: drgonfly at ultranet.com (Marc Andelman) Date: Mon Mar 7 05:41:12 2005 Subject: Ph.D sales job Message-ID: <7gviel$6qh$1@antiochus.ultra.net> Biosource is the oldest recruitment firm in biotech, and an employer paid fee service. Our client, a large corporation, has a new division that sells reagents for high throughput screening and drug discover, such as, for example, mRNA screening. This company needs someone to manage their most important sales territory, centered in NJ and PA. The home base is Philadelphia. They would like to hire someone experienced in both molecular biology or biochemistry and sales. Interested people please E mail Marc Andelman at drgonfly@ultranet.com Unless specifically requested, please allow me only to get back in touch should this or some other position be a dead ringer for all concerned. Thank you, Marc Andelman Biosource inc. 1 Parkton Ave. Worcester, MA 01605 tel 508 853 8803 From iseljmmmau at BIGSKY.NET Sat May 8 01:52:59 1999 From: iseljmmmau at BIGSKY.NET (iseljmmmau@BIGSKY.NET) Date: Mon Mar 7 05:41:12 2005 Subject: Discount Email vhaxh Message-ID: <199905080652.XAA26027@net.bio.net> Are You Interested in Bulk E-Mail? Do You: Want a contract bulk mailer that delivers? Want access to the best e-mail lists available on the Internet? DO YOU NEED? Full version Bulk E-Mail software at a discount? SIMPLY THE VERY BEST! For more info, send email to xraybabe@realradiomail.com with "bulkinfo" in the subject box. PS: I have mailing software and list management tools at discount prices, better than 50% off list price. I also have general internet and targeted lists, NOT A*L, that have been cleaned and verified. These lists get 90% plus deliverables. 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However, if you wish to be removed from this promotion or any future mailings, just send an email to: clickhere ==================================================== >> From g.yip at INAME.COM Sat May 8 16:24:13 1999 From: g.yip at INAME.COM (g.yip@INAME.COM) Date: Mon Mar 7 05:41:12 2005 Subject: Detection of proteoglycans and glycosaminoglycans Message-ID: <9905081718451G.27028@weba7.iname.net> Hi! I am writing to ask if anyone is familiar with the "critical electrolyte concentration" phenomenon in the detection of different glycosaminoglycans using alcian blue solution on paraffin-embedded sections. The problem I have is that the alcian blue precipitates out of solution immediately upon the addition of magnesium chloride. Any advice would be most deeply appreciated. --------------------------------------------------- Get free personalized email at http://www.iname.com From schildkr at aecom.yu.edu Sat May 8 19:15:00 1999 From: schildkr at aecom.yu.edu (Dr. Carl Schildkraut) Date: Mon Mar 7 05:41:12 2005 Subject: *** POSTDOCTORAL FELLOW POSITION, DNA REPLICATION, NEW YORK *** Message-ID: <7h2k24$iea$1@moonbeam.aecom.yu.edu> POSTDOCTORAL FELLOW POSITION AVAILABLE A position is available to study the molecular genetics of replication and the cell cycle in the mammalian genome. Our laboratory has been studying the replication of the immunoglobulin heavy chain (Igh) gene locus in mouse and human cell lines. In a non-B cell line in which immunoglobulin genes are not expressed, we have identified a region of about 50 kb in which replication of the Igh locus initiates ( Mol. Cell. Biol. 17:6167, 1997; Mol. Cell ,3:321, 1999). We now plan to focus on this initiation region and determine if it is a sequence specific initiation site. The evidence we have thus far indicates that this is a very important site replication initiation site. We plan to determine whether the mammalian Origin Recognition Complex (ORC) binds to this site and to determine whether there is a pre-replication complex at this site. Importantly, we have demonstrated for the first time in mammalian cells that a gradual transition between early and a late replicated domains in the Igh locus is achieved by a single replication fork. This raises the possibility that the transition between other early and late replicating regions is also achieved in this manner. In preB cell lines, we are identifying a developmentally regulated replication origin for the Igh locus that appears to be used only when the locus is expressed. Using this system we plan to determine whether ORC binds to the same sequences in MEL cells and pre B cells even if the origins of DNA replication are silent in MEL cells. We will also determine whether specific proteins in cells of the B lineage play a role in regulating usage of this developmentally regulated replication origin, possibly by interacting with ORC in the pre-replication complex. We also use the Epstein-Barr virus genome as a model system for replication of the human and mouse genomes (Cell 58:527, 1989; Mol. Cell. Biol. 15:2893, 1995). We are developing an approach to use fluorescent antibodies to study initiation in pure populations of isolated EBV genomes. We will then use this approach to focus on the developmentally regulated replication origin in cells of the B lineage. We will use the EBV genome as well as the Igh locus to determine whether ORC binds to mammalian replication origins. Send curriculum vitae by both Email and FAX or Air Mail and the names of three references (including telephone, FAX and Email numbers) to: Dr. Carl Schildkraut, Professor Department of Cell Biology (CH 416) Albert Einstein College of Medicine 1300 Morris Park Ave. New York, New York 10461 Phone (718) 430-2097 FAX (718) 430-8574 Email schildkr@aecom.yu.edu From 93063454 at one2free.com.hk Sat May 8 11:17:30 1999 From: 93063454 at one2free.com.hk (Cindy Tong) Date: Mon Mar 7 05:41:12 2005 Subject: Help!!!! Message-ID: <7h1np7$ahr$1@imsp009a.netvigator.com> I got a problem recently. I have to count the mitotic index on the HeLa cell. I try to use propidium iodide to stain my HeLa cell. Then I use the fluorescent microscope to find out the cell in M phase. But the problem is that it is very difficult to distingush which cell is in M phase. Dose anyone who have the experience to count the mitotic index before? If so, please tell me what kind of method can I fix the cell on the slide? So what kind of staining dye is the best to stain the cell? From xhleaqzpji at CORPNEWS.COM Sun May 9 00:22:05 1999 From: xhleaqzpji at CORPNEWS.COM (xhleaqzpji@CORPNEWS.COM) Date: Mon Mar 7 05:41:12 2005 Subject: Explode Your Sales mydfe Message-ID: <199905090655.IAA26770@sl1.nrh.de> Are You Interested in Bulk E-Mail? Do You: Want a contract bulk mailer that delivers? Want access to the best e-mail lists available on the Internet? DO YOU NEED? Full version Bulk E-Mail software at a discount? SIMPLY THE VERY BEST! For more info, send email to secretz@realradiomail.com with "bulkinfo" in the subject box. PS: I have mailing software and list management tools at discount prices, better than 50% off list price. I also have general internet and targeted lists, NOT A*L, that have been cleaned and verified. These lists get 90% plus deliverables. If you are interested in any of the above, send reply email to the above address with "bulkinfo" in the subject box. -=-=-=-=- -=-=-=-=- Remove Instructions -=-=-=-=- -=-=-=-=- To be removed, send email to the above address and type remove in the subject box. Note!! If you complain and get the box cancelled, you are only hurting the people who want to be removed. From bladerunner3 at MESSAGEASAP.COM Sun May 9 06:20:53 1999 From: bladerunner3 at MESSAGEASAP.COM (bladerunner3@MESSAGEASAP.COM) Date: Mon Mar 7 05:41:12 2005 Subject: Finally Here ! Flat Rate Long Distance !!! Message-ID: <199905091120.EAA01192@net.bio.net> IT'S HERE!!! Flat Rate Long Distance UNLIMITED TIME $20 - $60 a month * NO Charge per Minute * NO Changing of Carriers * Month to Month Billing - NO Contract * Residential & Commercial * From Any Phone Call 310-393-3889 or Toll Free at: 1-877-FLAT-NEW (877-3528-639) It's about time! Unlimited time. If this e-mail was an inconvenience to you we appologize. We are a Member of National Organization of Internet Commerce. To have your name added to our universal remove list please go to remove@noic.org and the name will be added and distributed to all of our members. From no at BODY.COM Sun May 9 08:48:52 1999 From: no at BODY.COM (mr tam) Date: Mon Mar 7 05:41:12 2005 Subject: 턜�횟瑥웽a찿쨠�{,�豊��セ� $1500 ⒝쩑쨁 Message-ID: <419.436289.39210243no@body.com> �빱쉈q┳10�~켻㉵밴ㅼ쫆찿ㅞ쟐첦킠�_쫆찿쨠�{, 쫆찿촑そ굘쪿�~곌펤겋짫쨏,⒡쩑쿯샘뫈Ой쯻, 뎬�N¾ⅸ,�[쨁ⓦ냔쫐�~퇱톨켒푊쫆찿짩�� , Ⅲ�宣Q쩧�@촽쫆찿. 1) �@�탁韻利a찿퀂3�瀅隻� 퀂쨠�]�튕C�`샘 : 랸ㅼ ($48) Φ楸ㅼ($46) �黜棋�($45) Φ턌ㆊ($42) �帑Hㆊ($43) 떨�빱�($48) 2) 펧곌/텻쨓쬞킠�_쫆찿 퀂쨠�]�튕C�`샘 : 텅빛킠�_쫆찿 ($31) 밴ㅼ킠�_쫆찿 ($41) 흛わ쵵퉞�빱쉈q: 촨Ν (TEL:81090082) any inconvenience caused , please call me to delete it...sorry From nbryce at hotmail.com Mon May 10 07:29:19 1999 From: nbryce at hotmail.com (Nicole Bryce) Date: Mon Mar 7 05:41:12 2005 Subject: Chamber Slides Message-ID: <3736d3b3.0@occy.pnc.com.au> I was wondering if anybody knows who manufactures disposable glass chamber slides, suitable for growing cells in for immunohistochemistry and confocal microscopy? Thanks Nicole From das1002remove-this-bit at cam.ac.uk Mon May 10 09:16:10 1999 From: das1002remove-this-bit at cam.ac.uk (David) Date: Mon Mar 7 05:41:12 2005 Subject: signal transduction References: <3732CF1E.8F3CC2AE@hotmail.com> Message-ID: A new project run partly by the journal 'Science', called the 'Signal Transduction Knowledge Environment' , could be just what you need. It is due to start running this summer, and in the meantime you can register to be sent email when it will start, by going to the following WWW site: http://www.stke.org Regards, David In article <3732CF1E.8F3CC2AE@hotmail.com>, Martijn Veltmaat wrote: > Is there anybody outthere, who has a nice compilation scheme of all the > molecules and (faulty) signal transduction processes that go on in > cancer? I know it's a lot to ask for, but I hate to waste my time > preparing such a scheme, while it's already available. -- To reply, please remove the 'remove-this-bit' from my return email address (it is there to try to reduce the incidence of unwanted 'mass-emails'). From szabi1 at westel900.net Mon May 10 13:16:36 1999 From: szabi1 at westel900.net (Szabi) Date: Mon Mar 7 05:41:12 2005 Subject: warez References: <7fi899$mna$1@talia.mad.ttd.net> Message-ID: <37372284.38F0FE07@westel900.net> www.extra.hu/dr_c From jgalletti at my-dejanews.com Mon May 10 14:27:59 1999 From: jgalletti at my-dejanews.com (jgalletti@my-dejanews.com) Date: Mon Mar 7 05:41:12 2005 Subject: Special coverage on cryptochromes Message-ID: <7h7bvs$fca$1@nnrp1.deja.com> There has been a lot going on around cryptochromes for the last couple of weeks. These photoreceptors play a key role in the regulation of circadian rhythms. If you would like to know more about this discovery, and many more, just visit my site. I have a collection of free Cell Biology links that are available to everyone, which is weekly updated to reflect the rapid changes of this exciting field. Check it out! Jeremias' Alley http://www.geocities.com/CollegePark/Stadium/2142/index.htm Thanks for you time, Jeremias The Jeremias' Alley Webmaster --== Sent via Deja.com http://www.deja.com/ ==-- ---Share what you know. Learn what you don't.--- From nancieed at earthlink.net Mon May 10 12:27:22 1999 From: nancieed at earthlink.net (Nanci E Donacki) Date: Mon Mar 7 05:41:12 2005 Subject: Chamber Slides References: <3736d3b3.0@occy.pnc.com.au> Message-ID: <7h7fld$hs4$1@holly.prod.itd.earthlink.net> Nunc Nicole Bryce wrote in message <3736d3b3.0@occy.pnc.com.au>... >I was wondering if anybody knows who manufactures disposable glass chamber >slides, suitable for growing cells in for immunohistochemistry and confocal >microscopy? >Thanks Nicole > > From vum at np.edu.sg Mon May 10 21:48:48 1999 From: vum at np.edu.sg (Uma chrissie) Date: Mon Mar 7 05:41:12 2005 Subject: Help!!!! References: <7h1np7$ahr$1@imsp009a.netvigator.com> Message-ID: <37379a90.0@nsuxnews> "Cindy Tong" <93063454@one2free.com.hk> wrote: >I got a problem recently. I have to count the mitotic index on the HeLa>cell. I try to use propidium iodide to stain my HeLa cell. Then I use the >fluorescent microscope to find out the cell in M phase. But the problem is >that it is very difficult to distingush which cell is in M phase. Dose >anyone who have the experience to count the mitotic index before? If so, >please tell me what kind of method can I fix the cell on the slide? So what >kind of staining dye is the best to stain the cell? > Dear Cindy Tong, The most common fixative uesd is Carnoy's fixative which is basically 3parts methanol and 1 part acetic acid. Your cells have to be thoroughly washed in fixative before proceeding to staining. If you are only required to do a chromosome count without any banding then Giemsa stain is sufficient. Yours truly, Chrissie vum@np.edu.sg > From askayu at ihug.co.nz Tue May 11 02:41:14 1999 From: askayu at ihug.co.nz (Charles Yu) Date: Mon Mar 7 05:41:13 2005 Subject: Helps need urgently!!!! Message-ID: <3737DF1A.99300F36@ihug.co.nz> Can anyone give me some ideas on the essay topic " How would you use 'bioprospecting' to identify and isolate novel enzymes from microorganisms???" If u know how to do it,can u please write to the following email address: askayu@ihug.co.nz Thanx!!!! >From Charles Yu From tobias.weiss at arcormail.de Tue May 11 04:45:05 1999 From: tobias.weiss at arcormail.de (Tobias Weiss) Date: Mon Mar 7 05:41:13 2005 Subject: Doktorandenstelle Message-ID: <7h8u5p$q2k$1@rznews.rrze.uni-erlangen.de> Wir sind eine dynamische Arbeitsgruppe, die sich mit der Bestimmung und Bewertung von Schadstoffen in menschlichen K�rperfl�ssigkeiten (z.B. Pestizide, PAH, ...) befa�t. Wir suchen eine/n Naturwissenschaftler/in als Doktoranden/in (BATIIa/2) der/die sich unter Anwendung der modernen Methoden der instrumentellen Analytik (GC/MS/-MS, HPLC) wissenschaftliche Meriten erwerben m�chte. Bewerbungen erbeten an: Prof. Dr. J. Angerer, Institut f�r Arbeits-, Sozial- und Umweltmedizin (Dir.: Prof. Dr. Dr. G. Lehnert) Schillerstra�e 25, 91054 Erlangen. -- ------------------------------------------------------------------------- Tobias Weiss Dipl. Chem. Institut f�r Arbeits-, Sozial- und Umweltmedizin Universit�tsstra�e 42 91052 Erlangen Phone/Fax: +49 9131 85-26272 tobias.weiss@rzmail.uni-erlangen.de www.arbeitsmedizin.uni-erlangen.de ------------------------------------------------------------------------- From hbdi at idt.net Tue May 11 07:40:09 1999 From: hbdi at idt.net (NDRI/HBDI) Date: Mon Mar 7 05:41:13 2005 Subject: Autopsy, Surgical Discard and Transplantation Tissues for Now Available for Research Message-ID: <7h98f3$f5j@nnrp4.farm.idt.net> See www.ndri.com for access to human tissues and organs for medical researchers See www.hbdi.org for information about family collections containing DNA and immortalized cells - over 600 families - for medical and genetic researchers NDRI and HBDI are non-profit organizations aiding medical research and the search for disease genes. From campbesg at TCD.IE Tue May 11 10:19:43 1999 From: campbesg at TCD.IE (Susan Campbell) Date: Mon Mar 7 05:41:13 2005 Subject: 3'end processing analysis Message-ID: Dear All, Could anybody tell me the most efficient way that I may assay mutants for 3'end processing. Thanks, Susan Susan Campbell, PhD Student, Moyne Institute of Preventive Medicine, Microbiology Department, Trinity College Dublin, Dublin 2, Ireland. TEl: 353-1-6082521 FAX: 353-1-6799294 From ggermino at welch.jhu.edu Tue May 11 13:48:49 1999 From: ggermino at welch.jhu.edu (ggermino@welch.jhu.edu) Date: Mon Mar 7 05:41:13 2005 Subject: Post-doc Position Message-ID: <37383315.97583B83@welchlink.welch.jhu.edu> Post-Doctoral Position Available July, 1999. The laboratory of Greg Germino is seeking a candidate to assist in the identification and characterization of the gene mutated in human autosomal recessive polycystic kidney disease. It is predicted that the gene product is likely to function as a key regulator of later stages of renal and hepatic development. Thus, the ideal candidate will have prior experience in the fields of developmental and/or cell biology. The position is not an NIH-training grant position and thus all candidates with the desired scientific background are encouraged to apply. Interested candidates should forward a CV, cover letter and the names of three references either by e-mail, fax or surface mail to Greg Germino, Johns Hopkins University School of Medicine, Ross 970, 720 Rutland Avenue, Baltimore, MD 21205. Telephone 410-614-1650, FAX 410-614-5129, e-mail: ggermino@welch.jhu.edu Selected Recent References: 1) Qian, F., Watnick, T. J., Onuchic, L. F., and Germino, G. G. The molecular basis of focal cyst formation in human autosomal dominant polycystic kidney disease type I. Cell 87:979-87, 1996. 2) Qian, F., Germino, F. J., Cai, Y., Zhang, X., Somlo, S., and Germino, G. G. PKD1 interacts with PKD2 through a probable coiled-coil domain. Nat Genet 16:179-83, 1997. 3) Watnick, T. J., Torres, V. E., Gandolph, M. A., Qian, F., Onuchic, L. F., Klinger, K. W., Landes, G., and Germino, G. G. Somatic mutation in individual liver cysts supports a two-hit model of cystogenesis in autosomal dominant polycystic kidney disease. Mol Cell 2:247-51, 1998 From cohlberg at csulb.edu Tue May 11 16:23:37 1999 From: cohlberg at csulb.edu (Jeffrey A. Cohlberg) Date: Mon Mar 7 05:41:13 2005 Subject: Biochemistry lecturer position Message-ID: <37389FD9.BDE@csulb.edu> Opening for a Full-Time Lecturer, 1999-2001 California State University, Long Beach Department of Chemistry and Biochemistry Duties: Lecturing in introductory and intermediate biochemistry courses and supervision of biochemistry laboratory courses. Opportunities may also be available for lecturing in graduate level biochemistry courses and supervision of biochemistry colloquia for senior undergraduate and MS students. Fall 1999 classes begin on August 30. Salary commensurate with qualifications and experience. Qualifications: Ph. D. in Chemistry or Biochemistry or a closely related field. Ability to communicate effectively with a diverse campus community. Prior successful teaching experience preferred. Required Documentation: Letter of application; resume; three letters of recommendation; official transcript from institution awarding highest degree. Position open until filled (or recruitment canceled). Review of applications to begin May 15. Applications and required documentation should be addressed to: Dr. N. M. Senozan, Chair, Department of Chemistry and Biochemistry, 1250 Bellflower Blvd, Long Beach, CA 90840-3903. For more information contact Dr. Nail Sezozan (nsenozan@csulb.edu) or Dr. Jeffrey Cohlberg (cohlberg@csulb.edu). Equal Opportunity/Affirmative Action/Title IX Employer From nospamforsburg at SALK.EDU Tue May 11 20:26:20 1999 From: nospamforsburg at SALK.EDU (S L Forsburg) Date: Mon Mar 7 05:41:13 2005 Subject: CELL CYCLE MEETING Message-ID: <3738D7C2.E9BE05F5@salk.edu> The First Salk Institute Cell Cycle Meeting 18-22 June 1999 DEADLINE EXTENDED to May 14th 1999. Talks chosen from abstracts. Visit the web site at http://www.salk.edu/meetings/cellcycle.html for more information. See you there! -- -- -susan :;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:; DON'T REPLY to the email address in header. It's an anti-spam. Use the one below. :;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:; S L Forsburg, PhD forsburg@salk.edu Molecular Biology and Virology Lab The Salk Institute, La Jolla CA http://pingu.salk.edu/~forsburg/lab.html Women in Biology Internet Launch Page http://pingu.salk.edu/~forsburg/bio.html :;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:; "These are my opinions. I don't have time to speak for anyone else." :;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:; From whlsleco at BIGFOOT.COM Tue May 11 21:14:52 1999 From: whlsleco at BIGFOOT.COM (whlsleco@BIGFOOT.COM) Date: Mon Mar 7 05:41:13 2005 Subject: WHOLESALE PRODUCTS! Message-ID: <2519955D2319884.jjhy@flash.net> We are updating our mailing list. If you would like to receive more information concerning THOUSANDS OF WHOLESALE PRODUCTS, please reply with "ADD ME" in the subject field. Otherwise, you will not receive any further email from our company. All orders placed before 4PM EST are shipped same day from our California Warehouse. We carry several lines of product at wholesale cost for resale. Best Regards, Scott McClellan/CEO MCCLELLAN WHOLESALE CO., INC. 813-915-9328 ___________________________________________________________________________________________________ This is a one-time mailing. If you do not respond with "ADD ME", you will NOT be contacted again. Thank You. From factorsfunding at EXCITE.COM Wed May 12 03:19:01 1999 From: factorsfunding at EXCITE.COM (factorsfunding@EXCITE.COM) Date: Mon Mar 7 05:41:13 2005 Subject: adv:## Business Owners: Small Business Loans Message-ID: <465.694408.591262@199.0.65.17.tiac.net>

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From ericlan at sentron.com.hk Wed May 12 04:13:41 1999 From: ericlan at sentron.com.hk (EricLan) Date: Mon Mar 7 05:41:14 2005 Subject: =?big5?B?MTUwSyC2V7Cqs3S69KRXuaO4daxPpvO1pafWt1A/IA==?= Message-ID: <004f01be9c54$108e4de0$0900a8c0@192.168.0.100.Sentron> This is a multi-part message in MIME format. ------=_NextPart_000_0042_01BE9C96.F2452DC0 Content-Type: multipart/alternative; boundary="----=_NextPart_001_0043_01BE9C96.F255F6A0" ------=_NextPart_001_0043_01BE9C96.F255F6A0 Content-Type: text/plain; charset="big5" Content-Transfer-Encoding: quoted-printable =B7q=B1=D2=AA=CC =20 =BB=D5=A4U=A5i=B4=BF=B7Q=B9=B3=B9L150K = =B6W=B0=AA=B3t=A9=F3=BA=F4=A4W=B9=A3=B8u=ACO=A6=F3=B5=A5=A7=D6=B7P? = =AC=FC=B0=EA=BA=E6=A6W=B3q=B0T=B3]=B3=C6=BBs=B3y=B0=D3Multi-Tech=A9=D2=B1= =C0=A5X=AA=BA=A1iProxyServer=A1j=A5i=B9=EA=B2{=B1z=AA=BA=B9=DA=B7Q=A1CPro= xyServer=A4=BA=B8m=A4F3 =AD=D356 K = Modem=A1A=A6@=A5i=B4=A3=A8=D1=B1z=B9=DA=B4K=A5H=A8D=AA=BA150K = =B6W=B0=AA=B3t=A4W=BA=F4=A1I=B8=D5=B7Q=B9=B3, 150K = =ACO=A8=E4=A5LModem=AA=BA=A4T=AD=BF, = =B7=ED=A8=E4=A5L=A4H=C1=D9=A6b=BA=F4=A4W=B9=A1=A8=FC=C0=BD=B6=EB=A4=A7=AD= W=AE=C9, = =B1z=BBP=B1z=AA=BAProxyServer=A6=AD=A4w=A7=B9=A6=A8=A4F=A9=D2=A6=B3=A4u=A7= @=A1C =B7=ED=B5M=A1A=BFW=BC=D6=BC=D6=A4=A3=A6p=B2=B3=BC=D6=BC=D6, = =A6=B3=A7_=B7Q=B9L=BBP=B1z=AA=BA=A6P=A6=F1=A4=C0=A8=C9ProxyServer=AA=BA=B0= =AA=B3t=A7=D6=B7P=A9O? = =A7=B9=A5=FE=A5i=A5H=A1AProxyServer=AF=E0=C5=FD=A6h=A6=ECLAN=A4W=A5=CE=AA= =CC=A6P=AE=C9=A6b=BA=F4=A4W=B9=A3=B8u=A1B=A6=AC=B5o=B9q=B6l=A1C =C1`=AA=BA=A8=D3=BB=A1=A1A=B1=C4=A5=CEProxyServer=ABK=BE=D6=A6=B3=A5H=A4U= =A6U=B6=B5=C0u=C2I : =20 =83=DC 1. 150K bps =B6W=B0=AA=B3t=A9=F3=BA=F4=A4W=B9=A3=B8u! =83=DC 2. = =A4@=AD=D3ProxyServer=AC=DB=B5=A5=A9=F3=AC=B0=A8C=A6=ECLAN=A4W=A5=CE=AA=CC= =B4=A3=A8=D1=A4@=B3=A1Modem! =83=DC 3. = =AC=D9=ABo=AC=B0=A8C=A6=EC=A6P=A8=C6=B3=A3=B0t=A4=A9=A4@=B1=F8=B9q=B8=DC=AA= =BD=BDu=A4W=BA=F4=A9M=BFW=A5=DF=A4=AC=C1p=BA=F4=B1b=B8=B9=B5=A5=AA=BA=A4j= =B5=A7=B6}=A4=E4! ProxyServer = =ACO=AFu=A5=BF=A6b=AC=FC=B0=EA=BBs=B3y=AA=BA=AC=FC=B0=EA=B2=A3=AB~, = =B5L=BD=D7=BD=E8=B6q=A9M=C3=AD=A9w=A9=CA=B3=A3=A7=F3=B3=D3=A8=E4=A5L=BCt=B0= =D3=AA=BA=A5X=AB~=A1CProxyServer = =A6=DB=B1=C0=A5X=AB=E1=A1A=A7=F3=B9=F0=C0=F2=AE=ED=BAa, = =A6h=A6=B8=AC=B0=A6h=B6=A1=B5=DB=A6W=B1M=B7~=B9q=B8=A3=C2=F8=BBx=B1=C0=C2= =CB=AC=B0=A1=A7=AD=BA=BF=EF=B2=A3=AB~=A1=A8=A1C =A6=DC=A9=F3=B0=E2=BB=F9=A4=E8=AD=B1=A1AProxyServer = =A8C=A5x=AFS=BB=F9=A5u=BB=DDHK$6,988.00(=AD=EC=BB=F9HK$11,190)=A1A = =B5=B4=A4=A3=A8=FA=A5=A9=A1C=B3o=AD=D3=BB=F9=BF=FA=A4w=A5]=ACA=A4FProxySe= rver=A1B=B3s=B1=B5LAN=AA=BACable=A1B=B3n=A5=F3=A1B=A6w=B8=CB=A9M=A8=CF=A5= =CE=B0=F6=B0V(=A5]=ACA=A5D=BE=F7=A4=CE=A4=AD=AD=D3=A4u=A7@=AF=B8, = =C3B=A5~=A4u=A7@=AF=B8=A8C=AD=D3=A5[=A6=ACHK$100)=A1A=A6=D3=B5L=A5=F4=A6=F3= =A8=E4=A5L=B6O=A5=CE=A1A=AC=DB=ABH=A4w=A5O=BB=D5=A4U=B3=DF=A5X=B1=E6=A5~=A7= a=A1I=A5B=BAC=A1A=A4Z=A9=F3=A4=BB=A4=EB=A7=C0=ABe=C1=CA=B6R=AA=BA=C5U=AB=C8= =A1A=A7=F3=A5i=C0=F2=C3=D8=A4=E9=A5=BB=A6W=BCtMita DP-560 = =C5J=AEg=A5=B4=A6L=BE=F7=A4A=B3=A1=A1C=BB=DA=A6=B9=B8g=C0=D9=A7C=B0g=A4=A7= =AE=C9=A1A=AD=B0=A7C=A6=A8=A5=BB=B4=A3=B0=AA=B1z=AA=BA=A4u=A7@=AE=C4=B2v=A1= A=A5=BF=ACO=A8=E8=A4=A3=AEe=BDw=A4=A7=A8=C6=A1C=AC=DB=ABH=BB=D5=A4U=A4=A3= =B7|=BF=F9=B9L=B3o=AD=D3=B4=A3=A4=C9=B6Q=A4=BD=A5q=C4v=AA=A7=A4O=AA=BA=A4= j=A6n=BE=F7=B7|=A7a! =BD=D0=A7Y=A6=DC=B9q2751-1708=A9=CE9276-1930=BBP=A7=DA=C1p=B5=B8, = =A7A=A4]=A5i=A8=EC=B3X=20 http://www.multitech.com/products/proxyserver = =C6[=AC=DD=B2=A3=AB~=AEi=A5=DC=A1C =B6=B6=ADP=B0=D3=B8R=A1I =B7s=B3=D0=B9q=B8=A3=A6=B3=AD=AD=A4=BD=A5q =C2=C5=B6=AF=A9=FA(Eric Lan) =A6p=BB=D5=A4U=A4=A3=B7Q=A6A=A6=AC=A8=EC=A5=BB=A4=BD=A5q=AA=BA=AB=C5=B6=C7= =B9q=A4l=ABH=A5=F3=AA=BA=B8=DC, =BD=D0=A7=E2=BB=D5=A4U=AA=BAe-mail = =A6a=A7}=B6=C7=AFu=A8=EC27511378 = =A8=C3=B5=F9=A9=FA=A7R=B0=A3=BB=D5=A4U=A6b=A5=BB=A4=BD=A5q=AA=BA=AAA=B0=C8= =B9=EF=B6H=A6W=B3=E6=A4W=AA=BAe-mail =A6a=A7}=A1C ------=_NextPart_001_0043_01BE9C96.F255F6A0 Content-Type: text/html; charset="big5" Content-Transfer-Encoding: quoted-printable
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<7ha9d8$iro$1@news.iastate.edu> I am working with a plasmid which behaves abnormally at cool temperatures. This is a 2.96 kb pBluescript vector containing a highly-repetitive 1.6 kb cDNA insert. I have used the vector to transform E. coli strains XL1-Blue and DH5-alpha, by heat shock and electroporation. When the bacteria are kept at room temperature or warmer, I can digest out the 1.6 kb fragment as usual. However, when the bacteria containing the plasmid are cooled to 4 degrees Centigrade or frozen (including in glycerol stock), the plasmid digestion results in only a fragment of the original insert. Has anyone ever heard of a plasmid behaving in such a cold-labile way? Do you ahve any ideas about what is happening? We suspect the fact that the insert is highly repetitive is important. I have more information if anyone is interested. I appreciate any input that I may receive. Thank you. Beth Winters ekw2@iastate.edu -- Beth Winters ekw2@iastate.edu "We drift down time, clutching at straws; but what good's a brick to a drowning man?" (Tom Stoppard) From un691cs at genius.embnet.dkfz-heidelberg.de Wed May 12 09:12:55 1999 From: un691cs at genius.embnet.dkfz-heidelberg.de (CSC) Date: Mon Mar 7 05:41:14 2005 Subject: cold-labile plasmid In-Reply-To: <7ha9d8$iro$1@news.iastate.edu> References: <7ha9d8$iro$1@news.iastate.edu> Message-ID: On 11 May 1999, Elizabeth K Winters wrote: > I am working with a plasmid which behaves abnormally at cool > temperatures. This is a 2.96 kb pBluescript vector containing a > highly-repetitive 1.6 kb cDNA insert. I have used the vector to > transform E. coli strains XL1-Blue and DH5-alpha, by heat shock and > electroporation. > When the bacteria are kept at room temperature or warmer, I can digest out > the 1.6 kb fragment as usual. However, when the bacteria containing the > plasmid are cooled to 4 degrees Centigrade or frozen (including in > glycerol stock), the plasmid digestion results in only a fragment of the > original insert. > Has anyone ever heard of a plasmid behaving in such a cold-labile way? > Do you ahve any ideas about what is happening? We suspect the fact that > the insert is highly repetitive is important. > I have more information if anyone is interested. I appreciate any input > that I may receive. Thank you. It isn't so much the plasmid which is behaving in this manner: it is the E. coli. Every bacterium has a recombination system which allows them to rearrange their DNA in times of duress. Cold is stress to the cell: the machinery is more active than usual. I have noticed myself several times that plasmids recombine if the cells are stored at 4C. Glycerol stocks still contain active bacteria, even at -70C. They can recombine nicely too (best strategy is to add less glycerol, f.i. only 5-10%). This is also true for RecA(-) strains, although they may be a little better at keeping inserts. clemens From no at BODY.COM Wed May 12 11:21:45 1999 From: no at BODY.COM (KONG WAY COMMUNICATION SERVICES LTD.) Date: Mon Mar 7 05:41:14 2005 Subject: CHEAPEST IDD TO CHINA Message-ID: <419.436293.01886597no@body.com> CHEAPEST IDD CALL TO CHINA: SHENZHEN �`�` HK$1.44 WITHIN GUANGTUNG 펣찯зㅊ쫆겅 HK$2.24 OUTSIDE GUANGTUNG 펣찯з�~쫆겅 HK$4.16 FOR DETAIL OR TO SUBSCRIBE, PLEASE CALL 2550 7142 From whlsleco at HOTMAIL.COM Thu May 13 11:16:42 1999 From: whlsleco at HOTMAIL.COM (whlsleco@HOTMAIL.COM) Date: Mon Mar 7 05:41:14 2005 Subject: WHOLESALE PRODUCTS! Message-ID: <2552235D2118884.jjhy@flash.net> We are updating our mailing list. If you would like to receive more information concerning THOUSANDS OF WHOLESALE PRODUCTS, please reply with "ADD ME" in the subject field. Otherwise, you will not receive any further email from our company. All orders placed before 4PM EST are shipped same day from our California Warehouse. We carry several lines of product at wholesale cost for resale. Best Regards, Scott McClellan/CEO MCCLELLAN WHOLESALE CO., INC. 813-915-9328 ___________________________________________________________________________________________________ This is a one-time mailing. If you do not respond with "ADD ME", you will NOT be contacted again. Thank You. From delenn at indigo.ie Thu May 13 14:41:32 1999 From: delenn at indigo.ie (Eimear Mullen) Date: Mon Mar 7 05:41:14 2005 Subject: Good graduate programs Message-ID: I am looking into the possibility of doing a graduate program in Cell biology and Genetics in the year 2000 Can anyone give me a list of good schools anywhere in the world? 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And I WILL DO IT...MASSIVE SPILLOVER will create MASSIVE incomes! Join now...... because everybody who joins after you will go straight to your downline! For more information send e-mail request for more Info: Mailto:totalabundance@usa.net?subject=Traditional Chinese P.S. This business alone can easily pay you a full-time living wage! Don't miss your chance to get in right near the top. From ricardo.bringas at CIGB.EDU.CU Thu May 13 20:12:43 1999 From: ricardo.bringas at CIGB.EDU.CU (Ricardo Bringas Perez) Date: Mon Mar 7 05:41:15 2005 Subject: BioInformatica Habana 99 Message-ID: An Introductory course on BioInformatics/ Curso Introductorio de BioInformatica DNA and protein sequences analysis/ Analisis de secuencias de ADN y proteinas. June 21-25, 1999, Centro de Ingenieria Genetica y Biotecnolgia (CIGB) La Habana, Cuba Organized by CIGB en EMBnet, visit: http://www.cigb.edu.cu/bioinfo99/ for more details and to fill your application form. contact us: BioInfo99@cigb.edu.cu From dechesne at unice.fr Fri May 14 03:14:06 1999 From: dechesne at unice.fr (Claude A. Dechesne) Date: Mon Mar 7 05:41:15 2005 Subject: One year post-doctoral position in Nice, France Message-ID: <7hgm0e$oi2$1@samoa.unice.fr> ĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿ Claude A. Dechesne ĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿĿ� One year post-doctoral position in Nice, France. Applications are invited for a post-doctoral position to work on characterization of novel muscle genes identified by suppression subtractive hybridization. A good background in molecular biology/cellular biology is required. This temporary position is for a non-French citizen, starting on January 1st, 2000. Salary before tax will be 12, 500 frs per month (about $2000) or more depending on experience. Position dependent on funding. Please send a curriculum vitae and names of two referees to: Dr. Claude A. Dechesne UMR CNRS 6548 Facult� des Sciences Parc Valrose 06108 Nice cedex 2 France Fax +33 (0)4 92 07 68 50 E-mail dechesne@unice.fr From hbdi at idt.net Fri May 14 09:54:32 1999 From: hbdi at idt.net (NDRI/HBDI) Date: Mon Mar 7 05:41:15 2005 Subject: Human Tissues, Organs and DNA from Families for Medical Research Message-ID: <7hhdfr$199@nnrp2.farm.idt.net> See www.ndri.com for access to human tissues and organs for medical researchers See www.hbdi.org for information about family collections containing DNA and immortalized cells - over 600 families - for medical and genetic researchers NDRI and HBDI are non-profit organizations aiding medical research and the search for disease genes. From 915608 at candseek.com Fri May 14 13:39:02 1999 From: 915608 at candseek.com (915608@candseek.com) Date: Mon Mar 7 05:41:15 2005 Subject: JOBOP ANIMAL SURGERY/HISTOLOGY Message-ID: <199905141838.LAA25103@net.bio.net> Since your email address was listed on a related web site page or database, I thought you might help. I am seeking an individual within the following conditions: Seeking a Research Assistant who will be working in a lab that is testing efficacy of novel pharmaceuticals in rodent models of ischemic and inflammatory disease. Responsibilities will include animal handling, performing small animal surgery and hypodermic drug administration. You will also be responsible for histological preparation of animal tissues, immunohistochemistry, myeloperoxidase assay, computer-assisted image analysis, ELISA, and animal behavioral testing. The candidate should possess a B.S. or M.S.degree in the biological sciences. Our client is a leading biotech firm with research facilities in Baltimore, Maryland and can provide excellent benefits (health insurance, dental, and vision plan, paid vacation and more). A high impact, high profile position with excellent opportunity for advancement. Geographic Location of Position: US-MD If you know anyone that might be interested, please forward this to them or contact: Stan Seaver DMC Voice: 609-584-8733 Ext. 218 Fax: 609-584-9575 Email: 915608@candseek.com To permanently discontinue receiving employment opportunity notices from any and all help wanted advertisers using the Candidate Seeker system, click your "Reply" button and type the word "re- move" without spaces between the letters into the SUBJECT field then click the "Send" button. Your email address will be permanently filtered from ALL future job opportunity notifications sent via the Candidate Seeker system. To temporarily filter employment opportunity notices sent via the Candidate Seeker system, type the acronym "JOBOP" into your subject filter. All employment opportunity notices sent via the Candidate Seeker system contain the acronym "JOBOP" in the subject so they may be easily filtered or blocked if so desired. Other email addresses may be permanently deleted from future contact by emailing a single blank message from the desired address to nomail@candseek.com. Enter additional addresses into the body of the message and they will also be added to the "nomail" list Please feel free to contact the candidateseeker.com feedback line at 609-584-5499. Do not use this number for job related questions. All job related questions should be directed to the employer by replying to contact addresses or phone numbers indicated at the end of the job description message. From fzhou at acsu.buffalo.edu Fri May 14 16:34:10 1999 From: fzhou at acsu.buffalo.edu (Fengquan Zhou) Date: Mon Mar 7 05:41:15 2005 Subject: help of LPA needed Message-ID: <373C96D4.12BFA9E7@acsu.buffalo.edu> It is well known that LPA (lysophosphatidic acid) can promote the stress fiber formaiton. However, I found in my experiment that LPA precipitates in the culture medium. The technique support person of the company I got my LPA told me it will precipitate if calcium is present in the medium. But the cell I use is not healthy in calcium free medium (I think most cells are). If any of you have experience of using LPA, your advise is highly appreciated. Is there a way to get around it or the LPA I got has bad quality? Thanks again! Joe fzhou@acsu.buffalo.edu From stockprofits900pc at GO2NET.COM Sat May 15 05:34:15 1999 From: stockprofits900pc at GO2NET.COM (stockprofits900pc@GO2NET.COM) Date: Mon Mar 7 05:41:15 2005 Subject: Possible +900% Stock Investment Return! Message-ID: <199905151034.DAA15007@net.bio.net> As Stock Investing Interests you, please CAREFULLY Review the Following - you will NOT be Disappointed! ========================================== "RNTK" Predicted Returns +900% over Next Few Months! ========================================== Imminent Outstanding Company News & Profit Potential Too Great to Ignore Dear Investor, Analysts have predicted a +900% rise in RNTK stock over the next few months due to recent developments and IMMINENT company news. RNTK (Rentech, Inc.) is the developer, marketer and licensor of PATENTED technology for the conversion of gasses (and liquids and solids) into super clean high value fuels, products and chemicals. In other words, RNTK makes it's money from selling PATENTED technology that BILLION Dollar Gas & Oil Corporations (such as Texaco) CANNOT do without. The technology converts otherwise waste, into valuable clean burning fuels while reducing greenhouse gas emissions and operating costs. 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We have working capital and meet the net tangible asset requirements for continued NASDAQ listing." **"BCP's excellent reputation and experience aligns Rentech with an engineering partner in an area of the world that could hold some of the greatest potential for Gas-To-Liquids technology" -- *Mr., Yakobson, CEO Rentech, Inc. (RNTK) Careful investigation will indicate our research is accurate, and will reveal RNTK's phenomenal profit potential - it will generate returns far greater than any other stock or investment program. As a buying frenzy is anticipated over the next few days, we advise you to act promptly to maximize returns. Understandably you may prefer to simply watch the stock, however the stock price being so low provides you with a very rare opportunity for enormous profits truly too great to miss. We recommend holding RNTK stock for at least 6 months until the promotion of the new patented technology is complete. ======================================================= This information is published by VSP when our research indicates particular stock will generate significant returns - this is where we put OUR money however it is not an endorsement to purchase any stock or security. To receive all further issues for a one-time fee of US$150, please reply to this email with "INFO" in subject, otherwise you will not be contacted again. Our 1998 stock picks generated in excess of 980% profit - statistics verified upon request. Sent on behalf of VSP Financial by Growth Success Strategies. VSP Financial currently owns 20,000 RNTK common stock From lisamyers2000 at BIGFOOT.COM Sun May 16 10:31:12 1999 From: lisamyers2000 at BIGFOOT.COM (lisamyers2000@BIGFOOT.COM) Date: Mon Mar 7 05:41:15 2005 Subject: Review any book, PC or mac software pgm, consumer products and keep them! Message-ID: <199905161531.IAA11068@net.bio.net> We are celebrating our 50th issue of our iNet Reviews Newsletter iNet Reviews Weekly News Brief May 15,1999 Contents: Book, software, & Consumer Product reviewers needed Top on-line editions of national magazines Best seller book list Some interesting articles in on-line magazines Hot web sites, topics & trends Anatomy of an adult web site - how to create one Webmaster Free Link offer Need Marketing Reps - use your computer to earn referral income New web camera site This is a trial issue of our free opt-in weekly newsletter. The purpose of this eclectic newsletter is to alert you to Interesting news and information on the web. -------------------------------------------- ITEM #01: iNet Reviews needs book, software, consumer product and movie reviewers. You may request any product to review and we have the publisher or manufacturer ship it to your home at no charge. You write a brief review and post it to our web site and you keep the review title. We also need individuals to receive free movie review passes to your local theater to review new movies. For your free reviewer information kit please reply to this email message or to lisamyers2000@bigfoot.com with the words STARTER 515B in the message subject line. LEAD STORIES Shadow in the Shallows The Navy's next nuclear submarine will lurk stealthily offshore, using advanced sensors and weaponry to attack land targets. And it doesn't need a periscope www.popsci.com If you like this article, you must read Sherry Sontag's Blind Man's Bluff - the untold story of American Submarine espionage. -------------------------------------------- ITEM #02: Top on-line editions of national magazines. 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MP3 is the fastest growing segment of the web site. What is MP3? This is a new file compression that is the standard for compressing music on the Internet. It gives you near CD-Quality music that you can download. The music publishers hate it with a passion are trying to do everything they can to squash it. So you must get a inexpensive CD-ROM writer ($199 after rebate at www.compusa.com) and start downloading your favorite tunes and making your own mix a copy them down to your own CD. I have a cable modem and can download them fast and have currently created 15 audio CD's with my favorite tunes. No longer do you have to lay out $15 for a CD to get one good song and 11 other fillers. ANATOMY OF AN ADULT WEB SITE - OR HOW TO START MAKING $750,000 PER MONTH IN SEVEN DAYS. We have seen the pre-release copy of this title and it is sure to be a best seller. It comes on a CD-ROM complete with hundreds of examples from the leading and most profitable adult web sites. 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Your free review starter kit will be mailed the day we receive your Self Addressed Stamped Envelope. ************* End of iNet Reviews **************** This is an opt-in short weekly newsletter designed to alert you to some mainstream news aspects of the Internet and an eclectic mix of interesting web topics, trends & topics. To be removed from our email database please email a message to lisamyers2000@bigfoot.com with the single word REMOVE in the message subject line. Our software will remove your email address from our database. This is an opt-in newsletter trial issue. We do add all remove request to our local remove database. From x98-cgu at nada.kth.se Sun May 16 14:48:33 1999 From: x98-cgu at nada.kth.se (Christian Guttmann) Date: Mon Mar 7 05:41:15 2005 Subject: ION - Channels Definition&Task Message-ID: <373F2111.7E580B1C@nada.kth.se> Hi, since I have to know something about neural cells, I would be very grateful if you can help me with the following. * Should I understand Ion channels as channels, which are in the membran of a cell and responsible for transportation of ions, like Na+, K+, Ca2+, Mg2+, Cl- and HCO-3, whereby the sodium and podassium channels are the most important? They are responsible for the concentration of the ions of both sides of the membran. * The main significance for nerve cells of K+ channels is the fast depolarization of the Actionpotential, for Na+ channels - the fast repolarization and the regulation of the firerate and for Ca+ - medium depolarization and a long-term Actionpotentials. Thanx for help Christian -- Christian Guttmann Mobile Tel. : +46-(0)70 414 99 76 Professorsslingan 19-119 Email : guttmann@gmx.de 10405 Stockholm Home Page : www.student.nada.kth.se/~x98-cgu/ Sweden From x98-cgu at nada.kth.se Sun May 16 15:22:26 1999 From: x98-cgu at nada.kth.se (Christian Guttmann) Date: Mon Mar 7 05:41:15 2005 Subject: Simulation of a nerve cell Message-ID: <373F2902.2186E06B@nada.kth.se> How to go about when one wishes to simulate a nerve cell on which biological data are available? Christian -- Christian Guttmann Mobile Tel. : +46-(0)70 414 99 76 Professorsslingan 19-119 Email : guttmann@gmx.de 10405 Stockholm Home Page : www.student.nada.kth.se/~x98-cgu/ Sweden From x98-cgu at nada.kth.se Sun May 16 15:12:28 1999 From: x98-cgu at nada.kth.se (Christian Guttmann) Date: Mon Mar 7 05:41:15 2005 Subject: Repolarization of an Actionpotentials Message-ID: <373F26AC.4049E4DA@nada.kth.se> Hi again, A repolization implies to have a depolarization first? But I don't know which two biophysical mechanism lie behind this phenomena? Well, the first could be, because of the switch between the inactive to the open configuration of the ion channels? Is the repolarization important because there can't be a backward Actionpotential (running to the opposite)? And also the maximum frequency can be limited dependent on the actionpotentials, is'nt it? chris' -- Christian Guttmann Mobile Tel. : +46-(0)70 414 99 76 Professorsslingan 19-119 Email : guttmann@gmx.de 10405 Stockholm Home Page : www.student.nada.kth.se/~x98-cgu/ Sweden -------------- next part -------------- An embedded message was scrubbed... From: Christian Guttmann Subject: ION - Channels Definition&Task Date: Sun, 16 May 1999 21:48:33 +0200 Size: 1525 Url: http://iubio.bio.indiana.edu/bionet/mm/cellbiol/attachments/19990516/7c194716/attachment.txt From x98-cgu at nada.kth.se Sun May 16 15:18:15 1999 From: x98-cgu at nada.kth.se (Christian Guttmann) Date: Mon Mar 7 05:41:15 2005 Subject: Chemical synapse: What happens there? Message-ID: <373F2807.F33AD2C@nada.kth.se> The question is how can an action potential, which reaches the presynaptic terminal of a chemical synapse, cause a transmittor release? My answer would be: If this happens, the vesicels in the synapse would get closer to the membran, fusionate, release most of there transmitters and finally getting back to the intracellular area. Right or Wrong? Another question: What is the difference between excitatory and inhibatory transmittor(examples)? And what determines if a cell has the excitatory or inhibitatory effect? I think the first questions could be that, i.g. Serotonin and glycin are inhibitatory transmittors and petins, like endorphine are excitatory??? Second questions ???? Do you have an answer? -- Christian Guttmann Mobile Tel. : +46-(0)70 414 99 76 Professorsslingan 19-119 Email : guttmann@gmx.de 10405 Stockholm Home Page : www.student.nada.kth.se/~x98-cgu/ Sweden From x98-cgu at nada.kth.se Sun May 16 15:28:12 1999 From: x98-cgu at nada.kth.se (Christian Guttmann) Date: Mon Mar 7 05:41:15 2005 Subject: functional Organization of the motor system of a higher vertebrate Message-ID: <373F2A5B.B409CB86@nada.kth.se> What are the main components of a functional Organization of the motor system of a higher vertebrate ? Christian -- Christian Guttmann Tel. : +46-(0)70 414 99 76 Professorsslingan 19-119 Email : guttmann@gmx.de 10405 Stockholm Home Page : http://www.student.nada.kth.se/~x98-cgu/ Sweden From x98-cgu at nada.kth.se Sun May 16 15:35:26 1999 From: x98-cgu at nada.kth.se (Christian Guttmann) Date: Mon Mar 7 05:41:15 2005 Subject: Myelinization Message-ID: <373F2C0E.125A7267@nada.kth.se> Mhm, I know that this substance improves Actionpotentials, but how. What magic kind of stuff is this? How does it exactly work? Thank you a lot for you help? I really looking forward to here from you!! Christian -- Christian Guttmann Tel. : +46-(0)70 414 99 76 Professorsslingan 19-119 Email : guttmann@gmx.de 10405 Stockholm Home Page : http://www.student.nada.kth.se/~x98-cgu/ Sweden From x98-cgu at nada.kth.se Sun May 16 15:25:24 1999 From: x98-cgu at nada.kth.se (Christian Guttmann) Date: Mon Mar 7 05:41:15 2005 Subject: Modular Organization in the celebral cortex Message-ID: <373F29B4.BBFCDCC6@nada.kth.se> There are two different types of modules in the primal visual cortex? There are dominant and orientating areas. How do they work? Somebody could help me? Christian -- Christian Guttmann Mobile Tel. : +46-(0)70 414 99 76 Professorsslingan 19-119 Email : guttmann@gmx.de 10405 Stockholm Home Page : www.student.nada.kth.se/~x98-cgu/ Sweden From faxblast at MAILANDNEWS.COM Sun May 16 19:13:56 1999 From: faxblast at MAILANDNEWS.COM (FaxBlaster) Date: Mon Mar 7 05:41:15 2005 Subject: (AD)--Fax Advertising Works!!-- -FWGD Message-ID: <199905170013.RAA05171@net.bio.net> All our mailings are sent complying to the proposed United States Federal requirements for commercial e-mail: Section 301 Paragraph (a)(2)(C) of S. 618. -To be removed from all future mailings simply send an email to --- removeme@webmaster.tj and put "REMOVE" in the subject line. mailto:removeme@webmaster.tj?subject=REMOVE ***************************************************************** !!!!!!!!***** FAX ADVERTISING WORKS *****!!!!!!!! *** It's Our Business To Make Your Business Better *** Would you like to reach thousands of potential costomers directly? -Fax advertising can bring your message directly into thier office. 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Our prices beat all our competition by at least 15%. If you would like more info then please send an e-mail to: faxblaster@webmaster.tj and put "FAX BLAST" in the subject line mailto:faxblaster@webmaster.tj?subject=FaxBlast Thank You and Best Wishes Sincerely Jobe Caster From gary at purdue.edu Sun May 16 23:12:48 1999 From: gary at purdue.edu (Gary Jasdzewski) Date: Mon Mar 7 05:41:15 2005 Subject: Myelinization References: <373F2C0E.125A7267@nada.kth.se> Message-ID: In article <373F2C0E.125A7267@nada.kth.se>, Christian Guttmann wrote: >Mhm, I know that this substance improves Actionpotentials, but >how. What magic kind of stuff is this? How does it exactly work? > >Thank you a lot for you help? > >I really looking forward to here from you!! > >Christian Myelin is fat (Schwann cells) that coat an axon the more active it is. The effect of this insulating fat on the axon's ability to conduct a signal is similar to the effect of good insulation on a speaker wire. The signal is less likely to be interfered with by other signals and it is also less likely to "leak" out before reaching the synapse. I find it humorous that learning is really just putting fat on in the right places! -- gary jasdzewski gary@purdue.edu From bjoern at brembs.net Mon May 17 01:05:21 1999 From: bjoern at brembs.net (Bjoern Brembs) Date: Mon Mar 7 05:41:16 2005 Subject: ION - Channels Definition&Task References: <373F2111.7E580B1C@nada.kth.se> Message-ID: <373FB1A1.324B8912@brembs.net> Christian Guttmann wrote: > * Should I understand Ion channels as channels, which are in the membran > of a cell > and responsible for transportation of ions, like Na+, K+, Ca2+, Mg2+, > Cl- and HCO-3, > whereby the sodium and podassium channels are the most important? They > are responsible > for the concentration of the ions of both sides of the membran. I would think so. Any textbook on neurobiology (even just biology) should contain this basic information- > * The main significance for nerve cells of > K+ channels is the fast depolarization of the Actionpotential, for > Na+ channels - the fast > repolarization and the regulation of the firerate and for Ca+ - > medium depolarization and a > long-term Actionpotentials. It should be all within one chapter of any biology textbook. Bjoern From bjoern at brembs.net Mon May 17 01:10:44 1999 From: bjoern at brembs.net (Bjoern Brembs) Date: Mon Mar 7 05:41:16 2005 Subject: Chemical synapse: What happens there? References: <373F2807.F33AD2C@nada.kth.se> Message-ID: <373FB2E4.8341E1FD@brembs.net> Christian Guttmann wrote: > The question is how can an action potential, which reaches the > presynaptic terminal of a chemical synapse, cause a transmittor > release? Until now, nobody really knows. The "pope" in this area is Thomas Suedhoff. Look for reviews by him in "medline" or some other reference databank. You'll learn that there are numreous proteins involved in docking and fusion of the vesicels to the presynaptic membrane. Contact me privately if you have problems finding the references (one of the groups in our department are working on this issue). > Another question: What is the difference between excitatory and > inhibatory transmittor(examples)? Different from taxon to taxon. Many inhibitory transmitters in vertebrates are excitatory in invertebrates. And I think even within an animal that can be different between CNS and PNS (not so sure about that one, though). > And what determines if a > cell has the excitatory or inhibitatory effect? It depends on what the transmitter receptor in the subsynaptic membrane does: de- or hyperpolarization. Bjoern From bjoern at brembs.net Mon May 17 01:12:43 1999 From: bjoern at brembs.net (Bjoern Brembs) Date: Mon Mar 7 05:41:16 2005 Subject: Simulation of a nerve cell References: <373F2902.2186E06B@nada.kth.se> Message-ID: <373FB35B.567EF42F@brembs.net> Christian Guttmann wrote: > How to go about when one wishes to simulate a nerve cell on which > biological data are available? For some standard simulations there should be software available. In all other cases: learn as much as you can about neurons and write a simulation in whatever programming language you like :-) Sorry, can't halp you more on that one. Bjoern From bjoern at brembs.net Mon May 17 01:17:24 1999 From: bjoern at brembs.net (Bjoern Brembs) Date: Mon Mar 7 05:41:16 2005 Subject: Repolarization of an Actionpotentials References: <373F26AC.4049E4DA@nada.kth.se> Message-ID: <373FB474.FAC37B6F@brembs.net> Christian Guttmann wrote: > A repolization implies to have a depolarization first? Japp. > But I don't know which two biophysical mechanism lie behind > this phenomena? You have active and passive transportation of ions through membranes (intra- and extracellularly) in both cases. I would presume that repolarisation requires more ATP than depolarization. For the exact processes, any neurobiology textbook should do. > Is the repolarization important because there can't be > a backward Actionpotential (running to the opposite)? > And also the maximum frequency can be limited dependent > on the actionpotentials, is'nt it? Japp. The refractory period is the limiting factor for maximum stimulation frquency and prevents backward propagation of actionpotentials. Bjoern From potik at hotmail.com Mon May 17 02:08:34 1999 From: potik at hotmail.com (Gary Potikyan) Date: Mon Mar 7 05:41:16 2005 Subject: Myelinization References: <373F2C0E.125A7267@nada.kth.se> Message-ID: myelinization increases the speed of the action potential along an axon. Look in a biology textbook and you'll find nice pictures of how myelin wraps arround the axon and only leaves small regions of "naked" axon. These are called Nodes of Ranvier and this is where the sodium/potassium pumps are doing their stuff. In other words, the electrical potential is generated by the difference in the overall charge inside and outside the membrane. Since the myelin sheath is covering some of the regions of the axon membrane, the electrical potential must jump over the myelin to the next Node of Ranvier. This significantly increases the speed of action potentials. Multiple sclerosis is believed to be caused by immune cells invading the nerves and destroying the myelin sheaths. This causes a significant decrease in speed of electric potentials along the axons, thus triggering a variety of symptoms. Hope this helped. If you need me to clarify any of this, feel free to email me. kpotikya@ucsd.edu Christian Guttmann wrote in message news:373F2C0E.125A7267@nada.kth.se... > Mhm, I know that this substance improves Actionpotentials, but > how. What magic kind of stuff is this? How does it exactly work? > > Thank you a lot for you help? > > I really looking forward to here from you!! > > Christian > > > -- > Christian Guttmann Tel. : +46-(0)70 414 99 76 > Professorsslingan 19-119 Email : guttmann@gmx.de > 10405 Stockholm Home Page : http://www.student.nada.kth.se/~x98-cgu/ > Sweden > > > From jarnie at utu.fi Mon May 17 10:06:35 1999 From: jarnie at utu.fi (Jarmo Niemi) Date: Mon Mar 7 05:41:16 2005 Subject: Myelinization References: <373F2C0E.125A7267@nada.kth.se> Message-ID: In article <373F2C0E.125A7267@nada.kth.se> Christian Guttmann writes: >Mhm, I know that this substance improves Actionpotentials, but >how. What magic kind of stuff is this? How does it exactly work? ...and several questions of similar kind. Sorry, but would it not be better, if you read an introductory text on neurophysiology first ? The answers are not likely to be of much help to you. To quote an old song about Watergate: "...if you didn't know _that_, man, what else don't you know ?" Myelin is an isolator formed from cell membranes. It works the same way as coaxial cable; the nerve impulse traverses the isolated section as an electrical, rather than electrochemical pulse. -- Jarmo Niemi jarnie@utu.fi http://www.utu.fi/~jarnie/ From potik at hotmail.com Mon May 17 02:32:55 1999 From: potik at hotmail.com (Gary Potikyan) Date: Mon Mar 7 05:41:16 2005 Subject: Chemical synapse: What happens there? References: <373F2807.F33AD2C@nada.kth.se> Message-ID: Vesicles filled with the neurotransmitter are in the terminal region of the presynaptic neuron. When the action potential reaches the terminal region of this neuron, calcium channels open and calcium rushes into the cell from the surrounding. It is thought that the calcium binds a protein called synapsin I which is a protein that acts as a bridge between the vesicle and the cytoskeleton. When calcium binds synapsin I, it releases the vesicle and the vesicle moves toward the membrane and ultimately binds it. The contents of the vesicle are dumped into the synaptic cleft. (Please note that the above mechanism is still experimental and it involves many other proteins and cellular components that I haven't listed). The neurotransmitters bind the membrane receptors of the postsynaptic neuron. These membrane receptors (acetylcholine receptors, for example) are channels, which open upon binding with the neurotransmitter and allow the influx and eflux of sodium and potassium. This continues the action potential in the next neuron. The type of postsynaptic membrane receptor determines if the neurotransmitter is excitatory or inhibitory. For example, acetylcholine can be excitatory if it binds a beta 1 adrenergic receptor. It has inhibitory effects when it binds beta 2 adrenergic receptors. So the type of action is mainly due to the type of receptor on the postsynaptic cell. Good luck Gary Potikyan University of California, San Diego kpotikya@ucsd.edu -------------------------------------------- Christian Guttmann wrote in message news:373F2807.F33AD2C@nada.kth.se... > The question is how can an action potential, which reaches the > presynaptic terminal of a chemical synapse, cause a transmittor > release? > > My answer would be: > If this happens, the vesicels in the synapse would get closer to the > membran, fusionate, release most of there transmitters and > finally getting back to the intracellular area. > Right or Wrong? > > Another question: What is the difference between excitatory and > inhibatory transmittor(examples)? And what determines if a > cell has the excitatory or inhibitatory effect? > > I think the first questions could be that, i.g. Serotonin and glycin are > > inhibitatory transmittors and petins, like endorphine are excitatory??? > Second questions ???? > > Do you have an answer? > > -- > Christian Guttmann Mobile Tel. : +46-(0)70 414 99 76 > Professorsslingan 19-119 Email : guttmann@gmx.de > 10405 Stockholm Home Page : www.student.nada.kth.se/~x98-cgu/ > Sweden > > > From vintbp at singnet.com.sg Mon May 17 13:42:29 1999 From: vintbp at singnet.com.sg (Cari) Date: Mon Mar 7 05:41:16 2005 Subject: DNA Message-ID: <7hpo2k$pb8$1@mawar.singnet.com.sg> Which is the dominant form of DNA in cells? A,B or Z form? From jybrown at utmb.edu Mon May 17 15:16:04 1999 From: jybrown at utmb.edu (Judith Y.-Brown, Ph.D.) Date: Mon Mar 7 05:41:16 2005 Subject: TX/US Microinjectionist needed Message-ID: <3740788D.42ABBAD7@utmb.edu> This is a multi-part message in MIME format. --------------F6AB0A0ECB18F8DC53E9D478 Content-Type: text/plain; charset=us-ascii; x-mac-type="54455854"; x-mac-creator="4D4F5353" Content-Transfer-Encoding: 7bit Gene-Cell, Inc. Gene-Cell, Inc. is a small publicly traded (OTCBB: GCLL) biotechnology company engaged in the development of innovative products for use in gene therapy. We are located in the Texas Clear Lake area on the Gulf of Mexico, equidistant from downtown Houston and Galveston. The location provides for easy access to two major medical centers (the University of Texas Medical Branch, Galveston and the University of Texas Health Science Center, Houston), NASA, and a growing community of biotechnology companies. Excellent schools, affordable housing, fishing, and recreational water sports make this an excellent location to live and work. We currently have an opening for an experienced microinjectionist: A BS, MS, or PhD in the areas of cell biology/biochemistry/molecular biology or equivalent is required. The individual should be well versed in the various methods of microinjection especially with glass needle-mediated microinjection. Experience with cell culture and molecular biology techniques is considered a plus but not a requirement. The individual will be responsible for performing experiments requiring glass needle-mediated microinjection technology, performing gene expression and cell viability experiments, maintaining various cell culture lines, and will also participate in planning and conducting experiments. Gene-Cell, Inc. provides a competitive benefits package including health care, retirement, and stock option programs. In addition, some relocation assistance will be provided. Salary is competitive and commensurate with experience. Starting date can be as early as June 1, 1999. Candidates are requested to e-mail, surface mail, or fax, in confidence, their resume and the names of 3 references to: Gene-Cell, Inc. Human Resources Department 1010 Hercules Ave. Houston, TX 77058 fax: 281-538-1948 genecell@phoenix.net Gene-Cell, Inc. is an equal opportunity employer. --------------F6AB0A0ECB18F8DC53E9D478 Content-Type: text/x-vcard; charset=us-ascii; name="jybrown.vcf" Content-Transfer-Encoding: 7bit Content-Description: Card for Judith Y.-Brown, Ph.D. Content-Disposition: attachment; filename="jybrown.vcf" begin:vcard n:Y.-Brown, Ph.D.;Judith x-mozilla-html:FALSE adr:;;;;;; version:2.1 email;internet:jybrown@utmb.edu x-mozilla-cpt:;1 fn:Judith Y.-Brown, Ph.D. end:vcard --------------F6AB0A0ECB18F8DC53E9D478-- From artax at shineline.it Sun May 16 21:31:08 1999 From: artax at shineline.it (Federico e Mario Bari) Date: Mon Mar 7 05:41:16 2005 Subject: project: site sur la recherche scientifique pour les lesions pinaux Message-ID: <373F7F6B.F86E9475@shineline.it> Mai 1999 Bonjour chers amis, nous sommes des ?tudiants de la province de Venise. Il y a quelque temps, nous travaillons ensemble ? un projet tr?s ardu mais tr?s passionant :nous cherchons de divulguer dans Internet, un site totalement non-profit o? pouvoir faire le point de la situation actuelle, sur la recherche scientifique pour les l?sions spinaux.Ce que nous cherchons, avant de publier notre site sur page web, est la collaboration directe des m?decins et des r?chercheurs qui s'interessent ? ce sujet, pour connaitre leurs opinions et les possibles innovations de la recherche.En particulier, nous demandons aux responsables des centres de recherche n?urobiologique qui s'interessent des l?sions spinaux, de tenir dans notre site une rubrique constamment mise au jour sur les progr?s de leur recherche. Notre initiativew, a eu l'egide de notre mairie et de notre province de Venise et on est intentionn?s meme ? demander celle de la Communaut? Europ?enne. Cette initiative est n?e car, voyageant dans Internet pour trouver des informations sur les l?sions ? la moelle ?pini?re, on s'est aper?u que ce qu'il y avait, ?taient des informations tr?s difficiles ? joindre,tr?s compliqu?es du point de vue de la compr?hension et tr?s dispers?es.On a donc pens? de construire nous memes un site facile ? atteindre, simple ? consulter et qui puisse regrouper toutes les informations n?c?ssaires pour donner un espoir raisonnable et non trompeur ? ceux qui ont subi un traumatisme meme emotif du ? la l?sion de la colonne vert?brale. Il y a une raison plus profonde que nous voit alli?s pour le meme but. Il s'agit de Giancarlo, un cher copain que, il y a 5 ans, ? subi une l?sion m?dullaire(c-4,c-5) pendant un match de rugby et qui depuis ce moment -l? est parapl?gique.Giancarlo aujourd'hui, est le plus enthousiaste de l'initiative , il travaille avec nous, et il esp?re dans la r?ussite de ce travail et surtout dans l'aide de ceux qui sauront cueillir l'intelligence et la juste cause de ce projet. Si vous pouvez de quelque mani?re nous aider dans ce projet vous pouvez le faire soit directement, par vos considerations ou avec des informations au sujet dont on parle, soit nous fournissant quelque adresse chez lequel nous pouvons rechercher une collaboration.Si vous desirez nous ?crire en fran?ais, sachiez que vous pouvez le faire, il y aura quelqu'un qui se chargera de traduire soigneusement votre travail.Nous comptons en effet, de traduire en plusieurs langues le materiel que nous arrivera en sachant que ce n'est pas facile de traduire les informations techniques ?crites en langage scientifique.C'est pour ?a qu'on demande aux m?decins d'etre simples dans les explications et compr?hensibles. Si vous avez des critiques,des suggestions ou simplement vous voulez savoir quelque chose de plus sur nous, vous pouvez nous ?crire ? ces adresses e-mail:gvolpato@shineline.it ,ou artax@shineline.it . Nous vous remercions de votre attention en esperant d'avoir bientot de vos nouvelles. From roshni at ccmb.ap.nic.in Tue May 18 05:50:17 1999 From: roshni at ccmb.ap.nic.in (Ms Roshni Mitra) Date: Mon Mar 7 05:41:16 2005 Subject: DNA References: <7hpo2k$pb8$1@mawar.singnet.com.sg> Message-ID: It is the B form Roshni --- From dgrd at loreal.com Tue May 18 06:32:45 1999 From: dgrd at loreal.com (DGRD) Date: Mon Mar 7 05:41:16 2005 Subject: L'OREAL Art and Science of Color Prize Message-ID: <7hrj3e$f0d$17@jaydee.iway.fr> ............................................................................................................................................................... L'OREAL Art and Science of Color Prize ............................................................................................................................................................... Does a scientist in your community deserve recognition? L'Oreal's Art and Science Foundation will award 60,000 euros in prizes for scientific research inspired by color. ......................................................................................................................................................................................................... Who is eligible? Scientists and artists of any nationality and any discipline who create and innovate in an area of color. ......................................................................................................................................................................................................... Prizes Grand Prize: one 30,000-euro prize ($32,600) Promotion Prize: two 15,000-euro prizes ($16,300 each) ......................................................................................................................................................................................................... Application period Deadline for application: July 31, 1999 Award ceremony: January 2000 in Paris, France ......................................................................................................................................................................................................... Jury An international jury of artists and scientists, including Nobel Prize winners, will select three winners whose work demonstrates a creative dialogue between art, science and color. ......................................................................................................................................................................................................... Questions? For more information, please contact: L'Oreal DGRD Direction de la communication Attention: Amandine de Francqueville Centre de Recherche Charles Zviak 90, rue du General Roguet 92583 Clichy Cedex France Telephone: +33 47 56 78 41 Fax: +33 47 56 41 85 E-mail: rd@loreal.com Web: http://www.loreal.com/art_and_science/colorprize.asp?ID=3 If you know a scientist working in a basic or applied discipline who may be eligible to enter, kindly forward him/her the following URL: http://www.loreal.com/art_and_science/colorprize.asp?ID=3 From dgrd at loreal.com Tue May 18 06:33:03 1999 From: dgrd at loreal.com (DGRD) Date: Mon Mar 7 05:41:16 2005 Subject: Know any scientists working on color? Message-ID: <7hrj41$f0d$18@jaydee.iway.fr> ............................................................................................................................................................... L'OREAL Art and Science of Color Prize ............................................................................................................................................................... Does a scientist in your community deserve recognition? L'Oreal's Art and Science Foundation will award 60,000 euros in prizes for scientific research inspired by color. ......................................................................................................................................................................................................... Who is eligible? Scientists and artists of any nationality and any discipline who create and innovate in an area of color. ......................................................................................................................................................................................................... Prizes Grand Prize: one 30,000-euro prize ($32,600) Promotion Prize: two 15,000-euro prizes ($16,300 each) ......................................................................................................................................................................................................... Application period Deadline for application: July 31, 1999 Award ceremony: January 2000 in Paris, France ......................................................................................................................................................................................................... Jury An international jury of artists and scientists, including Nobel Prize winners, will select three winners whose work demonstrates a creative dialogue between art, science and color. ......................................................................................................................................................................................................... Questions? For more information, please contact: L'Oreal DGRD Direction de la communication Attention: Amandine de Francqueville Centre de Recherche Charles Zviak 90, rue du General Roguet 92583 Clichy Cedex France Telephone: +33 47 56 78 41 Fax: +33 47 56 41 85 E-mail: rd@loreal.com Web: http://www.loreal.com/art_and_science/colorprize.asp?ID=3 If you know a scientist working in a basic or applied discipline who may be eligible to enter, kindly forward him/her the following URL: http://www.loreal.com/art_and_science/colorprize.asp?ID=3 From i.bell at btinternet.com Tue May 18 15:30:39 1999 From: i.bell at btinternet.com (SarahUKTV) Date: Mon Mar 7 05:41:16 2005 Subject: Help required with Osmotic Pressure Message-ID: <7hsihs$e4$1@uranium.btinternet.com> hi everyone, I am an A level Biology student in the UK. I have completed an assignment, but am left wondering about it's real-world applicability. A potato tuber cell is placed in a sucrose solution (the molarity of which, although irrelevant to my question here, is approx. 0.5 M), such that the cell is in osmotic equilibrium with the surrounding solution. Having worked out the equal (Psi) cell and (Psi) solution pressure in kiloPascals I am asked the biological significance of this information. My question is, how is this information applicable to real-life situations - if at all - in the home, industry, nature etc. Can anyone help me with this? Quoting examples would be particularly useful. Thanks James From muhero at globalnet.co.uk Tue May 18 17:33:53 1999 From: muhero at globalnet.co.uk (Graham Shepherd) Date: Mon Mar 7 05:41:16 2005 Subject: Help required with Osmotic Pressure References: <7hsihs$e4$1@uranium.btinternet.com> Message-ID: <7hsptg$2pi$1@gxsn.com> SarahUKTV wrote in message <7hsihs$e4$1@uranium.btinternet.com>... >hi everyone, > >I am an A level Biology student in the UK. I have completed an assignment, >but am left wondering about it's real-world applicability. > >A potato tuber cell is placed in a sucrose solution (the molarity of which, >although irrelevant to my question here, is approx. 0.5 M), such that the >cell is in osmotic equilibrium with the surrounding solution. Having worked >out the equal (Psi) cell and (Psi) solution pressure in kiloPascals I am >asked the biological significance of this information. > >My question is, how is this information applicable to real-life situations - >if at all - in the home, industry, nature etc. > >Can anyone help me with this? Quoting examples would be particularly useful. > >Thanks > >James > > You might ponder what insight this give into energy costs of synthesising storage molecules (starches, for example) compared to the energy required to maintain high concentrations of soluble nutrients inside the cell. Look at how methods of food preservation work - drying, salting, jam-making, for example: then look at methods of tissue preservation - blood for transfusion, organs for transplantation, plant tissue for propagation. You might also consider energy costs involved in desalinating water for drinking. GS From rishi.bhalerao at genfys.slu.se Wed May 19 08:28:11 1999 From: rishi.bhalerao at genfys.slu.se (RISHIKESH P. BHALERAO) Date: Mon Mar 7 05:41:16 2005 Subject: Two post-doc positions Message-ID: <3742BC67.759C273E@genfys.slu.se> Two Post-doctoral fellowships at the Dept of Forest Genetics and Plant Physiology in Umea, Sweden: We have two post-doc positions in our lab to work on the following projects: (I) The first position is in the area of cell cycle regulation during wood formation in Poplar. We have cloned cDNAs for several key cell cycle regulators from Poplar and are in the process of generating transgenic plants with modified levels of these cell cycle regulators in specific cell types in the wood forming tissues and assess the effect on wood formation in transgenic trees. (II) The second position is in the area of control of vascular development by IAA. We have cloned several genes rapidly upregulated by IAA and showing distinct tissue and cell type expression pattern in wood forming tissues of Poplar. The project focuses on elucidating the role of these IAA regulated genes in vascular development in Poplar and Arabidopsis. We are interested in employing highly motivated scientists with background in molecular biology and protein chemistry to work with us on the two projects. The projects involve collaboration with a team of scientists investigating wood formation using a variety of approaches involving analytical chemistry, microscopy and physiology. The work will involve construction and analysis of transgenic plants, cloning, expression analysis, in-situ hybridisation and analysis of protein phosphorylation. The positions are for 1+1 year starting as soon as possible for the applicant. The Dept of Forest Genetics and Plant Physiology employs over 70 scientists and students. We have sophisticated facilities for growth regulator measurements, confocal microscopy and several green houses. The city of Umea is a pleasent university town with excellent facilities for extracuricular activities with swimming pools, tennis and squash courts. In addition to the forestry department, the University of Umea has outstanding departments performing research in microbiology, animal and plant development. Interested individuals should send c.v. with name and addresses (fax and/or email) of three referees to: Dr. Rishikesh P. Bhalerao at the address below: Department of Forest Genetics and Plant Physiology The Swedish University of Agricultural Sciences S-901 83 Umea Sweden Tel:+46-90-7866282 Fax:+46-90-7865901 Email:rishi.bhalerao@genfys.slu.se From lyle at spam*cogni.iaf.cnrs-gif.fr*spam Wed May 19 13:49:01 1999 From: lyle at spam*cogni.iaf.cnrs-gif.fr*spam (Lyle Borg-Graham) Date: Mon Mar 7 05:41:17 2005 Subject: Simulation of a nerve cell References: <373F2902.2186E06B@nada.kth.se> <373FB35B.567EF42F@brembs.net> Message-ID: <7hud81$ek7$1@upsn21.u-psud.fr> Bjoern Brembs wrote: > > Christian Guttmann wrote: > > > How to go about when one wishes to simulate a nerve cell on which > > biological data are available? > > For some standard simulations there should be software available. > > In all other cases: learn as much as you can about neurons and write a > simulation in whatever programming language you like :-) > > Sorry, can't halp you more on that one. > > Bjoern GENESIS NEURON The Surf-Hippo Neuron Simulation System: http://www.cnrs-gif.fr/iaf/iaf9/surf-hippo.html From jcr8 at PSU.EDU Wed May 19 14:24:04 1999 From: jcr8 at PSU.EDU (Joe Reese) Date: Mon Mar 7 05:41:17 2005 Subject: Postdoctoral Position in Gene Regulation Message-ID: <37430EB8.4837C40D@psu.edu> A Postdoctoral position to study gene regulation in yeast is available in the Department of Biochemistry and Molecular Biology at Penn State University. The projects are aimed at understanding the role of the TBP-associated factors (TAFs) and chromatin in the regulation of cell cycle-regulated and DNA damage-inducible genes. (Nature 371:523; ibid. 383:185-188; Cell 90:607, ibid Cell 94:45). The highly motivated applicant should have an interest in gene regulation and have experience in molecular biology, biochemistry or yeast genetics. PSU has an exceptional group of investigators studying gene regulation in yeast and mammalian systems. http://www.personal.psu.edu/faculty/d/s/dsg11/GeneReg/psuGeneReg.htm. Applicants should send a CV, description of research interests and experience and the names and addresses of three references to: Dr. Joseph Reese, Dept. of Biochemistry and Molecular Biology, 203 Althouse Lab, Penn State University, University Park, PA 16802. email jcr8@psu.edu. From mark at going-going-sold.com Wed May 19 15:38:31 1999 From: mark at going-going-sold.com (Mark Atlas) Date: Mon Mar 7 05:41:17 2005 Subject: Internet auction and marketplace for used lab equipment Message-ID: <37432146.E6D13751@going-going-sold.com> Internet auction and marketplace for used lab equipment Please visit http://www.going-going-sold.com for the latest up to date auction listings. If the equipment you seek is not located on the auction block, you can also visit, http://www.going-going-sold.com/GGS/ggshome.nsf/LocFrameL?openform to place a request for competitive bids from all dealers, labs and lessors. The equipment bids will be sent back and you can choose from all of the equipment available.Over 140 requests for bids through the locator service have been submitted to Going,Going...Sold! We are currently ramping up our abilities to process all of the requests. Why spend your time shopping, we can do it for you better/faster and with our in lab evaluation of all purchases Recent price changes on the auction Saturn 1 GCMS lowered to $15,000. Includes 3400 and fully functional ion trap based GCMS form Varian. This is priced to sell. Biocad $22,000 Other specials not posted on this site: Fisons Kevex XRF, 3-5 yrs old, selling 50K Biomek 2000 1994(approximate)120K new selling 55K ABI 494 over 100K new selling 65K Excellent condidition If your lab has a number of small or large items which you wish to sell, we can now provide an easy to use software to allow you to create ads offline and email the software back to us.Please reply with a request for a copy of our new surplus management software. ***************************************************** - Recent Arrivals - Opening Prices ***************************************************** 1. Varian SpectraAA 600 Flame $9,750.00 ------------------------------------------------------------ 2. Varian SpectrAA 600Z furnace $11,500.00 ------------------------------------------------------------ 3. Oil Immersion Microscope $150.00 ------------------------------------------------------------ 4. Purifier/Clean Bench $300.00 ------------------------------------------------------------ 5. UV/Vis Spectrometer $7,000.00 ------------------------------------------------------------ 6. Protein/Peptide Sequencer 477A/PTH Analyzer 120A $9,500.00 ------------------------------------------------------------ 7. VWR 1630D Large Ovens $50.00 ------------------------------------------------------------ 8. Hand Held Refractometers $245.00 ------------------------------------------------------------ ***************************************************** Sale Closing : 5/5/99 Opening Prices ***************************************************** 1. Helium Displacement Pycnometer $4,500.00 ------------------------------------------------------------ 2. (New)Stereoscope Zeiss Stemi 2000 $2,150.00 ------------------------------------------------------------ 3. (New)Zeiss Stemi 1000 stereomicroscope $1,750.00 ------------------------------------------------------------ 4. Varian SpectraAA 600 Flame $9,750.00 ------------------------------------------------------------ 5. Varian SpectrAA 600Z furnace $11,500.00 ------------------------------------------------------------ 6. Waters LC Module 1 HPLC system and Millennium $16,000.00 ------------------------------------------------------------ 7. Tekmar 3000 Purge and trap $4,700.00 ------------------------------------------------------------ 8. PE Autosystem with dual ECD and autosampler $7,000.00 ------------------------------------------------------------ 9. MicroBalance $2,500.00 ------------------------------------------------------------ 10. Biocompatible Semi-Prep/Analytical HPLC w/Fraction Collector $32,000.00 ------------------------------------------------------------ 11. Biocad Preparative HPLC w/Fraction Collector $31,000.00 ------------------------------------------------------------ ***************************************************** Sale Closing : 5/7/99 Opening Prices ***************************************************** 1. Varian Saturn 1 GCMS $15,000.00 ------------------------------------------------------------ 2. Pharmacia LKB Wallac Liquid Scintillation Counter 1410-001 $5,920.00 ------------------------------------------------------------ 3. VG 70SE Magnetic Sector GCMS $95,000.00 ------------------------------------------------------------ 4. (new)Bright Field Microscope (Achromatic) $1,950.00 ------------------------------------------------------------ 5. (new) Leica ATC 2000 full phase microscope $2,700.00 ------------------------------------------------------------ 6. HP 5890 A with FID/Integrator 7673A autosampler $8,500.00 ------------------------------------------------------------ 7. ISO-DATA 20/10 GAMMA COUNTER $2,750.00 ------------------------------------------------------------ 8. Diode Array UV/VIS Spectrometer $6,200.00 ------------------------------------------------------------ 9. Solvent Recycler $2,500.00 ------------------------------------------------------------ 10. Varian GC-MS with P&T $10,500.00 ------------------------------------------------------------ 11. Waters HPLC Photo diode array system $4,800.00 ------------------------------------------------------------ 12. Oil Immersion Microscope $150.00 ------------------------------------------------------------ 13. Purifier/Clean Bench $300.00 ------------------------------------------------------------ 14. Miscellaneous items by the lot $200.00 ------------------------------------------------------------ 15. VWR 1630D Large Ovens $50.00 ------------------------------------------------------------ ***************************************************** Sale Closing : 5/12/99 Opening Prices ***************************************************** 1. Varian Spectra 10 Plus Atomic Absorbtion Flame and Furnace $6,500.00 ------------------------------------------------------------ 2. (New)Bright Field Microscope (PLAN) $2,950.00 ------------------------------------------------------------ 3. Coulter Counter model STKS $3,950.00 ------------------------------------------------------------ 4. Beckman J-21C and JA21 Rotor $3,000.00 ------------------------------------------------------------ 5. VORTEX FLOW PERFUSION BIOREACTOR $4,000.00 ------------------------------------------------------------ 6. AutoBalance( new 9 months) $5,000.00 ------------------------------------------------------------ 7. Air Cooled Chiller/heaters for mixers and fermentors $4,500.00 ------------------------------------------------------------ 8. Prosys Protein Purification system $2,000.00 ------------------------------------------------------------ 9. Varian Star 3400 w/FID,PID tekmar2000, data $7,750.00 ------------------------------------------------------------ 10. 42 cage Auto waterRat housing Rat racks $200.00 ------------------------------------------------------------ ***************************************************** Sale Closing : 5/14/99 Opening Prices ***************************************************** 1. HP 5890 GC $8,700.00 ------------------------------------------------------------ 2. New Olympus Microscope $1,600.00 ------------------------------------------------------------ 3. Polarizing Microscope $1,900.00 ------------------------------------------------------------ 4. Phase Contrast Microscope $2,000.00 ------------------------------------------------------------ 5. Plate washer $1,760.00 ------------------------------------------------------------ 6. (New) Infinity corrected Microscope system $3,900.00 ------------------------------------------------------------ 7. Water Jacketed Co2 Dual Stacked Incubator $2,750.00 ------------------------------------------------------------ 8. Blecha 120 environmental chamber $4,200.00 ------------------------------------------------------------ 9. PERKIN-ELMER LAMBDA 3 SPECTROPHOTOMETER $3,150.00 ------------------------------------------------------------ 10. UV/Vis Spectrometer $7,000.00 ------------------------------------------------------------ 11. Protein/Peptide Sequencer 477A/PTH Analyzer 120A $9,500.00 ------------------------------------------------------------ 12. Hand Held Refractometers $245.00 ------------------------------------------------------------ ***************************************************** Sale Closing : 5/19/99 Opening Prices ***************************************************** 1. Beckman DU-7 $3,500.00 ------------------------------------------------------------ 2. Beckman Gamma Counter $2,750.00 ------------------------------------------------------------ 3. Waters 680 Automated Gradient Controller for HPLC $1,460.00 ------------------------------------------------------------ 4. Fluoresence Microscope $4,800.00 ------------------------------------------------------------ 5. Superconducting NMR spectrometer $9,500.00 ------------------------------------------------------------ 6. Ultra-Low Temperature Freezer (-80c) 20.5 cu ft $2,400.00 ------------------------------------------------------------ 7. Waters Gradient HPLC $16,500.00 ------------------------------------------------------------ 8. Zymark SPE System $14,000.00 ------------------------------------------------------------ 9. ThermoSep HPLC System $14,500.00 ------------------------------------------------------------ 10. Quaternary HPLC w/ Data System $20,500.00 ------------------------------------------------------------ 11. Fisons MD-800 GCMS $27,800.00 ------------------------------------------------------------ 12. Waters PDA system with Millennium $25,900.00 ------------------------------------------------------------ ***************************************************** Sale Closing : 5/21/99 Opening Prices ***************************************************** 1. Leica Microscope $1,500.00 ------------------------------------------------------------ 2. Perkin Elmer AAS2100 Flame AA $12,000.00 ------------------------------------------------------------ 3. Lightweight sterilizer $3,600.00 ------------------------------------------------------------ 4. Varian GC-3400 $2,000.00 ------------------------------------------------------------ 5. Phase contrast Microscope w/camera $4,750.00 ------------------------------------------------------------ 6. 20 cubic foot Refridgerator $1,350.00 ------------------------------------------------------------ ***************************************************** (c) 1997, Internet Auctioneers International -------------- next part -------------- An HTML attachment was scrubbed... URL: http://iubio.bio.indiana.edu/bionet/mm/cellbiol/attachments/19990519/6139ca1b/attachment.html From mark at going-going-sold.com Wed May 19 15:38:49 1999 From: mark at going-going-sold.com (Mark Atlas) Date: Mon Mar 7 05:41:17 2005 Subject: Internet auction and marketplace for used lab equipment Message-ID: <37432159.9EFAD119@going-going-sold.com> Internet auction and marketplace for used lab equipment Please visit http://www.going-going-sold.com for the latest up to date auction listings. If the equipment you seek is not located on the auction block, you can also visit, http://www.going-going-sold.com/GGS/ggshome.nsf/LocFrameL?openform to place a request for competitive bids from all dealers, labs and lessors. The equipment bids will be sent back and you can choose from all of the equipment available.Over 140 requests for bids through the locator service have been submitted to Going,Going...Sold! We are currently ramping up our abilities to process all of the requests. Why spend your time shopping, we can do it for you better/faster and with our in lab evaluation of all purchases Recent price changes on the auction Saturn 1 GCMS lowered to $15,000. Includes 3400 and fully functional ion trap based GCMS form Varian. This is priced to sell. Biocad $22,000 Other specials not posted on this site: Fisons Kevex XRF, 3-5 yrs old, selling 50K Biomek 2000 1994(approximate)120K new selling 55K ABI 494 over 100K new selling 65K Excellent condition If your lab has a number of small or large items which you wish to sell, we can now provide an easy to use software to allow you to create ads offline and email the software back to us.Please reply with a request for a copy of our new surplus management software. ***************************************************** - Recent Arrivals - Opening Prices ***************************************************** 1. Varian SpectraAA 600 Flame $9,750.00 ------------------------------------------------------------ 2. Varian SpectrAA 600Z furnace $11,500.00 ------------------------------------------------------------ 3. Oil Immersion Microscope $150.00 ------------------------------------------------------------ 4. Purifier/Clean Bench $300.00 ------------------------------------------------------------ 5. UV/Vis Spectrometer $7,000.00 ------------------------------------------------------------ 6. Protein/Peptide Sequencer 477A/PTH Analyzer 120A $9,500.00 ------------------------------------------------------------ 7. VWR 1630D Large Ovens $50.00 ------------------------------------------------------------ 8. Hand Held Refractometers $245.00 ------------------------------------------------------------ ***************************************************** Sale Closing : 5/5/99 Opening Prices ***************************************************** 1. Helium Displacement Pycnometer $4,500.00 ------------------------------------------------------------ 2. (New)Stereoscope Zeiss Stemi 2000 $2,150.00 ------------------------------------------------------------ 3. (New)Zeiss Stemi 1000 stereomicroscope $1,750.00 ------------------------------------------------------------ 4. Varian SpectraAA 600 Flame $9,750.00 ------------------------------------------------------------ 5. Varian SpectrAA 600Z furnace $11,500.00 ------------------------------------------------------------ 6. Waters LC Module 1 HPLC system and Millennium $16,000.00 ------------------------------------------------------------ 7. Tekmar 3000 Purge and trap $4,700.00 ------------------------------------------------------------ 8. PE Autosystem with dual ECD and autosampler $7,000.00 ------------------------------------------------------------ 9. MicroBalance $2,500.00 ------------------------------------------------------------ 10. Biocompatible Semi-Prep/Analytical HPLC w/Fraction Collector $32,000.00 ------------------------------------------------------------ 11. Biocad Preparative HPLC w/Fraction Collector $31,000.00 ------------------------------------------------------------ ***************************************************** Sale Closing : 5/7/99 Opening Prices ***************************************************** 1. Varian Saturn 1 GCMS $15,000.00 ------------------------------------------------------------ 2. Pharmacia LKB Wallac Liquid Scintillation Counter 1410-001 $5,920.00 ------------------------------------------------------------ 3. VG 70SE Magnetic Sector GCMS $95,000.00 ------------------------------------------------------------ 4. (new)Bright Field Microscope (Achromatic) $1,950.00 ------------------------------------------------------------ 5. (new) Leica ATC 2000 full phase microscope $2,700.00 ------------------------------------------------------------ 6. HP 5890 A with FID/Integrator 7673A autosampler $8,500.00 ------------------------------------------------------------ 7. ISO-DATA 20/10 GAMMA COUNTER $2,750.00 ------------------------------------------------------------ 8. Diode Array UV/VIS Spectrometer $6,200.00 ------------------------------------------------------------ 9. Solvent Recycler $2,500.00 ------------------------------------------------------------ 10. Varian GC-MS with P&T $10,500.00 ------------------------------------------------------------ 11. Waters HPLC Photo diode array system $4,800.00 ------------------------------------------------------------ 12. Oil Immersion Microscope $150.00 ------------------------------------------------------------ 13. Purifier/Clean Bench $300.00 ------------------------------------------------------------ 14. Miscellaneous items by the lot $200.00 ------------------------------------------------------------ 15. VWR 1630D Large Ovens $50.00 ------------------------------------------------------------ ***************************************************** Sale Closing : 5/12/99 Opening Prices ***************************************************** 1. Varian Spectra 10 Plus Atomic Absorbtion Flame and Furnace $6,500.00 ------------------------------------------------------------ 2. (New)Bright Field Microscope (PLAN) $2,950.00 ------------------------------------------------------------ 3. Coulter Counter model STKS $3,950.00 ------------------------------------------------------------ 4. Beckman J-21C and JA21 Rotor $3,000.00 ------------------------------------------------------------ 5. VORTEX FLOW PERFUSION BIOREACTOR $4,000.00 ------------------------------------------------------------ 6. AutoBalance( new 9 months) $5,000.00 ------------------------------------------------------------ 7. Air Cooled Chiller/heaters for mixers and fermentors $4,500.00 ------------------------------------------------------------ 8. Prosys Protein Purification system $2,000.00 ------------------------------------------------------------ 9. Varian Star 3400 w/FID,PID tekmar2000, data $7,750.00 ------------------------------------------------------------ 10. 42 cage Auto waterRat housing Rat racks $200.00 ------------------------------------------------------------ ***************************************************** Sale Closing : 5/14/99 Opening Prices ***************************************************** 1. HP 5890 GC $8,700.00 ------------------------------------------------------------ 2. New Olympus Microscope $1,600.00 ------------------------------------------------------------ 3. Polarizing Microscope $1,900.00 ------------------------------------------------------------ 4. Phase Contrast Microscope $2,000.00 ------------------------------------------------------------ 5. Plate washer $1,760.00 ------------------------------------------------------------ 6. (New) Infinity corrected Microscope system $3,900.00 ------------------------------------------------------------ 7. Water Jacketed Co2 Dual Stacked Incubator $2,750.00 ------------------------------------------------------------ 8. Blecha 120 environmental chamber $4,200.00 ------------------------------------------------------------ 9. PERKIN-ELMER LAMBDA 3 SPECTROPHOTOMETER $3,150.00 ------------------------------------------------------------ 10. UV/Vis Spectrometer $7,000.00 ------------------------------------------------------------ 11. Protein/Peptide Sequencer 477A/PTH Analyzer 120A $9,500.00 ------------------------------------------------------------ 12. Hand Held Refractometers $245.00 ------------------------------------------------------------ ***************************************************** Sale Closing : 5/19/99 Opening Prices ***************************************************** 1. Beckman DU-7 $3,500.00 ------------------------------------------------------------ 2. Beckman Gamma Counter $2,750.00 ------------------------------------------------------------ 3. Waters 680 Automated Gradient Controller for HPLC $1,460.00 ------------------------------------------------------------ 4. Fluoresence Microscope $4,800.00 ------------------------------------------------------------ 5. Superconducting NMR spectrometer $9,500.00 ------------------------------------------------------------ 6. Ultra-Low Temperature Freezer (-80c) 20.5 cu ft $2,400.00 ------------------------------------------------------------ 7. Waters Gradient HPLC $16,500.00 ------------------------------------------------------------ 8. Zymark SPE System $14,000.00 ------------------------------------------------------------ 9. ThermoSep HPLC System $14,500.00 ------------------------------------------------------------ 10. Quaternary HPLC w/ Data System $20,500.00 ------------------------------------------------------------ 11. Fisons MD-800 GCMS $27,800.00 ------------------------------------------------------------ 12. Waters PDA system with Millennium $25,900.00 ------------------------------------------------------------ ***************************************************** Sale Closing : 5/21/99 Opening Prices ***************************************************** 1. Leica Microscope $1,500.00 ------------------------------------------------------------ 2. Perkin Elmer AAS2100 Flame AA $12,000.00 ------------------------------------------------------------ 3. Lightweight sterilizer $3,600.00 ------------------------------------------------------------ 4. Varian GC-3400 $2,000.00 ------------------------------------------------------------ 5. Phase contrast Microscope w/camera $4,750.00 ------------------------------------------------------------ 6. 20 cubic foot Refridgerator $1,350.00 ------------------------------------------------------------ ***************************************************** (c) 1997, Internet Auctioneers International -------------- next part -------------- An HTML attachment was scrubbed... URL: http://iubio.bio.indiana.edu/bionet/mm/cellbiol/attachments/19990519/377ad526/attachment.html From JSeagrav at LRRI.ORG Wed May 19 17:27:46 1999 From: JSeagrav at LRRI.ORG (Seagrave, JeanClare) Date: Mon Mar 7 05:41:17 2005 Subject: RESPIRATORY IMMUNOLOGY SYMPOSIUM Message-ID: Lovelace Respiratory Research Institute will present an international symposium focused on immune response in the respiratory system. The conference will be held in scenic Santa Fe, New Mexico, at the historic La Fonda Hotel, on October 10 - 13, 1999. The symposium will feature platform presentations by recognized experts in these fields, complemented by posters submitted in response to a call for abstracts. For more information on registration and abstract submission instructions, please visit our website: http://www.lovelace-symposium.org or contact: Alice Hannon AHANNON@LRRI.ORG Phone: (505) 845-1124 Fax: (505) 845-1193 From dots at cisnet.com Wed May 19 21:30:03 1999 From: dots at cisnet.com (Tom Dotson) Date: Mon Mar 7 05:41:17 2005 Subject: antibiotic chemistry Message-ID: <927167624.135.92@news.remarQ.com> Hello! A fellow student and I are researching the chemistry behind antibiotics and are looking for a little advice. Specifically, how does the antibiotic inhibitor bond with the enzymes that form the cell wall in bacteria? thanks for your help!! -tom Please email all responses to dots@cisnet.com From dots at cisnet.com Wed May 19 21:32:39 1999 From: dots at cisnet.com (Tom Dotson) Date: Mon Mar 7 05:41:17 2005 Subject: antibiotic chemistry Message-ID: <927167637.752.100@news.remarQ.com> Hello!! Just a real quick question to aid two students in their never ending quest for knowledge, or atleast help them with their research project! :) Specifically, how does the antibiotic inhibitor bond with the enzymes that form the cell wall in bacteria? thanks!!! tom Please email all responses to dots@cisnet.com From immune at intergate.bc.ca Thu May 20 13:12:36 1999 From: immune at intergate.bc.ca (immunechem) Date: Mon Mar 7 05:41:17 2005 Subject: antibiotic chemistry, beta-lactam as example References: <927167624.135.92@news.remarQ.com> Message-ID: <37444284@carrera.intergate.ca> The ring structure of beta-lactam antibiotics (penicillin type and ceflosporin type) contains a electrophillic carbon centre (the lactam carbon). transpeptidase which is required to crosslink the proteins for synthesis of the bacterial cell wall binds to bata-lactam antibiotics with high afffinity and reract with the beta-lactam antiobiotics chemically. The attack of its serine (transpeptidase active centre) hydroxyl group on the bata-lactam (intramolecular amide bond) carbonyl and form the caroxylic ester bond. The serine hydroxyl group, however, is also required for the transpeptidase for its enzyme activity. So when penicillin is covalently attached to the enzyme, its activity would be perminantly blocked therfore the bacterial can not produce cell wall and will die subsequently. The lactam carbonyl will also react to other nuclophiles such as primary amine in lysine residues to a less extent. th aatach of penicillin to the protein (conjugation) will trigger the immune response (allergic). The bacteria (resistant strain) could also produce an enzyme called beta-lactmase, which hydrolyze the inner amide (lactam) ring structure so that the antiobiotic will lose its reactivity to the transpeptidase. Today, beta-lactamase inhibitor plus bata-lactam antibiotics are utilized as good anti-bacterial cocktail. I hope this could help your student a little bit. In article <927167624.135.92@news.remarQ.com>, dots@cisnet.com says... > >Hello! > >A fellow student and I are researching the chemistry behind antibiotics and >are looking for a little advice. Specifically, how does the antibiotic >inhibitor bond with the enzymes that form the cell wall in bacteria? > >thanks for your help!! >-tom > >Please email all responses to dots@cisnet.com > > > From cijadra at zedat.fu-berlin.de Wed May 19 16:14:22 1999 From: cijadra at zedat.fu-berlin.de (Cijadrachon) Date: Mon Mar 7 05:41:17 2005 Subject: Myelinization References: <373F2C0E.125A7267@nada.kth.se> Message-ID: <3742f541.1777922@news.zedat.fu-berlin.de> >What magic kind of stuff is this? For some uses rather anti-magic stuff? ;-) ........ >> I find it humorous that learning is really just putting fat on in the right places! Why? (The "just" & the statement sounds wrong to me.) From jlove98905 at aol.com Fri May 21 00:54:11 1999 From: jlove98905 at aol.com (JLove98905) Date: Mon Mar 7 05:41:17 2005 Subject: Inducing Apoptosis? Message-ID: <19990521015411.07298.00003045@ng21.aol.com> Hi all- I am new here, this is my first post....so hello :o) I am trying to research different ways to induce apoptosis....I have decided to work with UV as fas ligand. i am wondering if anyone out there has any suggestions for protocols for exposing cells in culture to UV light? and how about a protocol using anti-fas? -jen (c) Copywight Elmer Fudd. All wights wesewved. From bradley at NORTHNET.NET Fri May 21 04:49:00 1999 From: bradley at NORTHNET.NET (Brad Henschel) Date: Mon Mar 7 05:41:17 2005 Subject: Work at home in your underwear Message-ID: <419.436301.19804387bradley@northnet.net> Hello! I apologize if this message has reached you in error. I recently purchased a list of people interested in a business opportunity and your address was on the list. Just delete this message and you will never hear from me again. I want to let you know about a very successful way to do business on the Internet. I own my own business and I have discovered that the best way to market my product is by simply letting my customers see it before they buy it. This concept is called shareware and it is available now thanks to the Internet. However, shareware is only cost effective when your product is information based. If you would like to see how this method of marketing works, you can check a site that offers FREE information using this concept. The site is http://www.secrets2success.com/special21110 Hoping this information to be helpful, From masao96 at HOTMAIL.COM Fri May 21 04:58:16 1999 From: masao96 at HOTMAIL.COM (ikjun kang) Date: Mon Mar 7 05:41:18 2005 Subject: (none) Message-ID: <19990521095414.79215.qmail@hotmail.com> Hi! I am a medical student in Seoul,Korea I am studying about APOPTOSIS. I want to know about molecular mechanism of apoptosis and it's role in viral pathogenesis. I will wait for your answer. Please help me! ______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com From ag24 at mole.bio.cam.ac.uk Fri May 21 07:43:37 1999 From: ag24 at mole.bio.cam.ac.uk (Aubrey de Grey) Date: Mon Mar 7 05:41:18 2005 Subject: Euromit IV, the Fourth European Meeting on Mitochondrial Pathology Message-ID: <7i3kdp$gep$1@pegasus.csx.cam.ac.uk> Dear Colleague, Euromit IV - Call for abstracts Euromit IV, the Fourth European Meeting on Mitochondrial Pathology, will be held at Queens' College, Cambridge on 16th-19th September 1999. This meeting will cover all aspects of mitochondrial disease, ranging from clinical pathology to the molecular mechanisms underlying the disease. This will be the fourth Euromit meeting in approximately ten years, all of which have been successful as witnessed by the increasing number of delegates and the acceptance of all invitations by the leading international investigators. Although various aspects of mitochondrial disease are catered for in other scientific meetings, Euromit is unique in retaining mitochondrial pathology as its focus. Mitochondrial disease is a burgeoning area of research interest. It is a particularly exciting time, as the underlying genetic defects are being reported not only for mitochondrial disorders associated with mutation of the mitochondrial genome, but also of the nuclear genome. Consequently, the meeting is timely. Euromit traditionally represents a forum for both clinicians and basic scientists and we welcome delegates from both sides of the clinical fence. Anyone who is interested should consult our web page for further information: http://www.gen.cam.ac.uk/euromit Abstracts should be submitted as detailed on the web page and should be sent to the organisers no later than 15th June 1999. Further queries which are not answered by the web page should be addressed to: Bob Lightowlers Reader in Molecular Neuroscience Dept of Neurology Medical School University of Newcastle upon Tyne Framlington Place Newcastle upon Tyne NE2 4HH UK Tel: (0044) 1 91 222 8028 Fax: (0044) 1 91 222 8553 e-mail: r.n.lightowlers@ncl.ac.uk From i.mcfarlane at icrf.icnet.uk Fri May 21 11:43:55 1999 From: i.mcfarlane at icrf.icnet.uk (Ian Mc) Date: Mon Mar 7 05:41:18 2005 Subject: Mouse thymus SDS-PAGE Message-ID: Hi, Does anyone have a protocol for preparing mouse thymus for SDS-PAGE. I just want to run a sample of the whole thing on a gel. Thanks Ian Mc From schildkr at aecom.yu.edu Sun May 23 20:15:07 1999 From: schildkr at aecom.yu.edu (Dr. Carl Schildkraut) Date: Mon Mar 7 05:41:18 2005 Subject: *** POSTDOCTORAL FELLOW POSITION, DNA REPLICATION, NEW YORK *** Message-ID: <7ia96r$saf$1@moonbeam.aecom.yu.edu> POSTDOCTORAL FELLOW POSITION AVAILABLE A position is available to study the molecular genetics of replication and the cell cycle in the mammalian genome. Our laboratory has been studying the replication of the immunoglobulin heavy chain (Igh) gene locus in mouse and human cell lines. In a non-B cell line in which immunoglobulin genes are not expressed, we have identified a region of about 50 kb in which replication of the Igh locus initiates (Mol. Cell. Biol. 17:6167, 1997; Mol. Cell ,3:321, 1999). We now plan to focus on this initiation region and determine if it is a sequence specific initiation site. The evidence we have thus far indicates that this is a very important site replication initiation site. We plan to determine whether the mammalian Origin Recognition Complex (ORC) binds to this site and to determine whether there is a pre-replication complex at this site. Importantly, we have demonstrated for the first time in mammalian cells that a gradual transition between early and a late replicated domains in the Igh locus is achieved by a single replication fork. This raises the possibility that the transition between other early and late replicating regions is also achieved in this manner. In preB cell lines, we are identifying a developmentally regulated replication origin for the Igh locus that appears to be used only when the locus is expressed. Using this system we plan to determine whether ORC binds to the same sequences in MEL cells and pre B cells even if the origins of DNA replication are silent in MEL cells. We will also determine whether specific proteins in cells of the B lineage play a role in regulating usage of this developmentally regulated replication origin, possibly by interacting with ORC in the pre-replication complex. We also use the Epstein-Barr virus genome as a model system for replication of the human and mouse genomes (Cell 58:527, 1989; Mol. Cell. Biol. 15:2893, 1995). We are developing an approach to use fluorescent antibodies to study initiation in pure populations of isolated EBV genomes. We will then use this approach to focus on the developmentally regulated replication origin in cells of the B lineage. We will use the EBV genome as well as the Igh locus to determine whether ORC binds to mammalian replication origins. Send curriculum vitae by both Email and FAX or Air Mail and the names of three references (including telephone, FAX and Email numbers) to: Dr. Carl Schildkraut, Professor Department of Cell Biology (CH 416) Albert Einstein College of Medicine 1300 Morris Park Ave. New York, New York 10461 Phone (718) 430-2097 FAX (718) 430-8574 Email schildkr@aecom.yu.edu From ivi at GOODMOOD.CO.UK Mon May 24 08:29:25 1999 From: ivi at GOODMOOD.CO.UK (ivi@GOODMOOD.CO.UK) Date: Mon Mar 7 05:41:18 2005 Subject: Having Trouble Getting A Credit Card?? Message-ID: <77271E7B16C6.AAC1C93@gate-iris.cy.coopers.com> Having Trouble Getting A Credit Card? You Won't Here............
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From office at selectscience.net Mon May 24 09:44:26 1999 From: office at selectscience.net (SelectScience) Date: Mon Mar 7 05:41:18 2005 Subject: Last Week's Chemistry & Life Science News Headlines Message-ID: <7ibmka$i0k$2@news1.cableinet.co.uk> Last week, www.selectscience.net reported on 31 news stories the day they happened, so if you need to keep up to speed on the latest business developments in chemistry and life science, make the news ticker on www.selectscience.net part of your daily routine. Andrew Smith (andrew@selectscience.net) Editor, www.selectscience.net Last week's headlines were: Peptide Therapeutics Appoints New Chairman Cantab Pharmaceuticals Reports First Quarter Financial Results and Significant Progress in Key Development Programs CuraGen Announces Completion of High Resolution Rat Genome Maps QIAGEN Announces 2-For-1 Stock Split and Conversion of Currency of its Par Value to EURO CLONTECH Receives ISO 9000 Certification Molecular Devices announces the Acquisition of Skatron Instruments AS Lonza Biologics and Genzyme Transgenics Corporation to Establish Strategic Alliance for Biopharmaceutical Production Millipore Acquires Bioprocessing Corp. QIAGEN Joins Valentis-DS Biologics Alliance for Manufacturing and Sales of Plasmid DNA Genmab Obtains Rights to IL15 Antibody Patents Roche Diagnostics Takes License Under Organon Teknika's Nucleic Acid Extraction Technology Vysis Reports Positive Q1 1999 Results Hemagen Announces 34% Increase in Sales From Same Quarter Last Year Protein Design Labs Announces Presentations of Clinical Results Using SMART Anti-CD3 Antibody Biogen Names Jennifer Jackson, Ph.D., as Director, Regulatory Affairs Life Technologies Completes Acquisition of Process Chromatography and Research Products Businesses from BioSepra IntegrLifeSciences Reports Results From 1999 Annual Meeting of Stockholders Steris Corporation to Acquire Assets of Ricerca, Inc. Biogen Names Michael Gilman, Ph.D., as Research Director of Molecular Biology ThermoQuest Announces First Quarter Results Thermo BioAnalysis Announces First Quarter Results Thermo Instrument Systems Announces First Quarter Results Thermo Optek Announces First Quarter Results Perkin-Elmer Sells Its Environmental And Process Analysis Division To SICK AG PE Corporation Declares Dividend Cerep SA Announces Drug Discovery Partnership with Pfizer New Scientific Advisory Board at Xenova Ontogen and Oxford Asymmetry International (OAI) Announce Collaboration Centocor Announces Senior Executive Appointment and Promotions Sale of Nycomed Pharma Completed Trinity Biotech Responds to Centocor Lawsuit From uncantcvhp at MONO.CITY.AC.UK Mon May 24 14:29:01 1999 From: uncantcvhp at MONO.CITY.AC.UK (uncantcvhp@MONO.CITY.AC.UK) Date: Mon Mar 7 05:41:18 2005 Subject: Discount Email aqceu Message-ID: <199905241254.SM00965@fcpug.mono.city.ac.uk> Are You Interested in Bulk E-Mail? Do You: Want a contract bulk mailer that delivers? Want access to the best e-mail lists available on the Internet? DO YOU NEED? Full version Bulk E-Mail software at a discount? SIMPLY THE VERY BEST! For more info, send email to secretz@realradiomail.com with "bulkinfo" in the subject box. I have bulk mailing software and list management tools at discount prices, better than 50% off list price. I also have general internet lists and Biz Opp targeted lists, NOT A*L, that have been cleaned and verified. These lists get 90% plus deliverables. If you are interested in any of the above, send email to the above address with "bulkinfo" in the subject box. -=-=-=-=- -=-=-=-=- Remove Instructions -=-=-=-=- -=-=-=-=- To be removed, send email to the above address and type remove in the subject box. Note!! If you complain and get the box cancelled, you are only hurting the people who want to be removed. From no.email.address.entered at none444.yet Mon May 24 22:44:20 1999 From: no.email.address.entered at none444.yet (no.email.address.entered@none444.yet) Date: Mon Mar 7 05:41:18 2005 Subject: $$$ WIN REAL MONEY $$$ - Default.htm 356 bytes (1/1) 53827 Message-ID: <05249921.4420@none444.yet> begin 644 Default.htm M/&AT;6P^#0H-"CQH96%D/@T*/'1I=&QE/G=I;CPO=&ET;&4^#0H\8F%S92!H M2!B M9V-O;&]R/2(C,# P,# P(B!T97AT/2(C,# P,$9&(CX-"@T*/&@Q(&%L:6=N M/2)C96YT97(B/D]V97(@/&9O;G0@8V]L;W(](B,P,$9&,# B/B0Q,# L,# P M/"]F;VYT/B!I;B!W:6YN:6YG Could you please define the following terms for me in regards to their role in the embryology/internal structure of an unfertilized chicken egg? Any help would be much appreciated. Please e-mail me. Michael 1. Blastodisc 2. White Yolk 3. Cord of twisted dense albumen 4. Air space 5. Inner shell membrane 6. Outer shell membrane 7. Yellow yolk 8. Shell 9. Dense albumen 10. Light albumen From ex03 at HOTMAIL.COM Mon May 24 23:11:52 1999 From: ex03 at HOTMAIL.COM (ex03@HOTMAIL.COM) Date: Mon Mar 7 05:41:18 2005 Subject: You're a Who's Who Candidate! Message-ID: Dear Candidate, You were recently selected by The Office of Public Affairs for a free listing on The International Executive Guild's Who's Who CD-ROM. Our Public Affairs Office gathers information from many recognized sources, including professional associations and societies, trade organizations, newspaper and magazine articles, professional reference publications, web presence, and referrals from existing members. As a highly respected professional in your field of expertise, we believe your contributions merit very serious consideration for inclusion on The International Executive Guild's Who's Who CD-ROM. To maintain the level of accuracy, we ask you to click on the web address highlighted below and fill out the brief bit of information required for inclusion. There is no cost or obligation to be listed on The International Executive Guild's Who's Who CD-ROM. All applicants will receive a free gift compliments of The International Executive Guild. http://208.26.69.200/members7/ex5543 Remember, this site is for executives, professionals, & entrepreneurs only! My Sincere Thanks, Lorraine A. Michaels Office Of Public Affairs For email list removal, please send an email w/ REMOVE in the subject field to: nomail01@quakemail.com From biohelp Tue May 25 04:00:18 1999 From: biohelp (BIOSCI Administrator) Date: Mon Mar 7 05:41:18 2005 Subject: BIOSCI/bionet miniFAQ & Fundraiser Message-ID: <199905250900.CAA03681@net.bio.net> (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From kesha at interrussia.com Tue May 25 06:09:54 1999 From: kesha at interrussia.com (Varlam B. Keshelava Ph.D.) Date: Mon Mar 7 05:41:18 2005 Subject: Metabolic Pathways eMap Message-ID: <374A8502.E3FA1EF4@interrussia.com> Hello all, I'm resident of Russia looking for collaboration and funding. My research may be interesting for biochemists, microbiologists, cell physiologists, bioinformatics, etc. research and education practice. The problem of biochemical information integration has been traditionally solved by Metabolic Pathways Maps arrangement. These maps (Michal 1982, Nicholson 1987) are large (up to 3 sq.m.) and entangled biochemical schemes. The scope of material covered and the tendency to present all the information at a flat paper inevitably result in an overloaded, complicated structure of the whole map. Puzzling out these mazes is rather difficult and, in fact, often useless. A convenient user friendly electronic (Internet) edition of Interactive Metabolic Pathways Map may be a very good support for biochemistry, physiology and related field research and teaching - this is the main goal of my project!. Now I have finished the basic preliminary study on the Metabolic Pathways Maze structure pattern. I invite any interested person to visit my site containing a short description of the results I have got: The Metabolic Pathways Maze has a clear and accessible structure!( if you fail to connect by clicking the link - type in your browser address line the following: http://www.interrussia.com/~kesha/biology/maze/index.htm ). I would appreciate any comments. I'm looking for contradictors, referees and collaboration! Thank you for attention. Varlam Keshelava PS Sorry for bad english - it is not my native language. From guilford at virginia.edu Tue May 25 10:50:53 1999 From: guilford at virginia.edu (William Guilford) Date: Mon Mar 7 05:41:18 2005 Subject: You're a Who's Who Candidate! References: Message-ID: <374AC6DD.6CED7809@virginia.edu> > You were recently selected by The Office of Public > Affairs for a free listing on The International Executive > Guild's Who's Who CD-ROM. Goodie. We've entered an age where newsgroups are recognized as corporeal beings. ;-) From ag24 at mole.bio.cam.ac.uk Tue May 25 11:02:38 1999 From: ag24 at mole.bio.cam.ac.uk (Aubrey de Grey) Date: Mon Mar 7 05:41:18 2005 Subject: Euromit IV - Registration schedule Message-ID: <7iehiu$gqo$1@pegasus.csx.cam.ac.uk> Dear Colleague, Euromit IV - Registration schedule As noted in a recent posting, Euromit IV, the Fourth European Meeting on Mitochondrial Pathology, will be held at Queens' College, Cambridge on 16th-19th September 1999. For details, please see our web page: http://www.gen.cam.ac.uk/euromit THIS POSTING IS TO DRAW YOUR ATTENTION TO THE FACT THAT THE DEADLINE FOR EARLY REGISTRATION IS APPROACHING. The registration fee for Euromit IV is �345 for students and �395 for others if received by 15 June 1999. This includes all sessions, accommodation and food, except for the Gala Dinner on the Saturday night. (The Gala Dinner is optional and costs a further �25.) Registration after 15 June 1999 costs an additional �50. You may register by mail, fax, email or directly on our web site. Further queries which are not answered by the web page should be addressed to: Bob Lightowlers Reader in Molecular Neuroscience Dept of Neurology Medical School University of Newcastle upon Tyne Framlington Place Newcastle upon Tyne NE2 4HH UK Tel: (0044) 1 91 222 8028 Fax: (0044) 1 91 222 8553 e-mail: r.n.lightowlers@ncl.ac.uk From reda at globalserve.net Tue May 25 12:31:12 1999 From: reda at globalserve.net (Danny Reda) Date: Mon Mar 7 05:41:18 2005 Subject: Redasoft Plasmid FREE Demo! Message-ID: <7iemp4$e0d$1@whisper.globalserve.net> Redasoft Plasmid lets you draw plasmids, vector maps and constructs quickly and easily. Circular and linear maps can be generated either automatically from sequence files (or from the web), or you can draw them manually. Plasmid has many useful and time-saving features and was reviewed in the May 21/99 issue of Science! Learn more about it at http://www.redasoft.com. From no.email.address.entered at none444.yet Wed May 26 00:07:11 1999 From: no.email.address.entered at none444.yet (no.email.address.entered@none444.yet) Date: Mon Mar 7 05:41:18 2005 Subject: XXX PAMELA ANDERSON VIDEO (FREE) - xxx.htm 602 bytes (1/1) 94853 Message-ID: <05259923.0711@none444.yet> begin 644 xxx.htm M/&AT;6P^#0H-"CQH96%D/@T*/'1I=&QE/GAX>"!6:61E;SPO=&ET;&4^#0H\ M8F%S92!H2!B9V-O;&]R/2(C,# P M,# P(B!T97AT/2(C1D8P,$9&(CX-"@T*/' @86QI9VX](F-E;G1E Mass spectrometers, sequencers, synthesizers, NMRs for sale: LC/Mass spectrometers: Sciex API III+ LC/MS/MS $ 69,000 ES, APCI, under PE service contract HP 1100 benchtop LC/MSD, APCI & API-electrospray, autosampler, DAD detector, < 1 year old, $130,000 Finnigan Navigator benchtop LC/MS: $ 75,000; 18 mos. old; factory refurb, install & 90-day warr. included Finnigan TSQ 7000 LC+GC/MS/MS: $ 97,500; current software; API-1 source; under Finnigan service contract Sciex 150 benchtop LC/MS: $ 98,000; 2 yrs old; includes Gilson 215 liquid handler, ELSD detector & HP 1100 HPLC. Finnigan SSQ 7000 LC/MS: $130,000; API-2 source, electrospray + APCI, Excaliber software, factory refurb, 90-day warranty, install included Micromass II: $50,000; ES, APCI, 3 yrs. old, includes HPLC, NOT Z-spray HP 5989B LC+GC/MS: $ 35,000; Extended mass range (2000 amu), hex ion guide; HPLC & warranty avail. Finnigan MAT 90: $30,000; Hi-resolution magnetic sector Fisons VG 2000: <$100,000 MALDI-TOFs: Hewlett-Packard G2025A $ 50,000 Peptide and oligo synthesizers and sequencers: ABI 394: $12,500 (Valve blocks rebuilt; warranteed) ABI 390Z: $4,000 (50-100uM yields) ABI 431: $12,500 (Rebuilt, warranteed) ABI 433: $19,000 (ABI upgrade) PerSeptive 9050+: $6,000 (As is/was working when decommissioned; add $3,500 for rebuild/warranty) ABI 373 stretch: $9,000 (Big dye upgrade; still under warranty) ABI 373 stretch: $7,000 (4-filter) ABI 377: $97,500 (XL; 96 lanes; transferrable ABI service contract) ABI 377: $60,000 (48-lane; under ABI contract) ABI Procise 492: $45,000 obo (ABI-certified) NMRs: 젨� Bruker DMX 600: $495,000; AVANCE; 3-axis gradients 젨� Varian Unity 500+: call for price; broadband 젨� Bruker AM360: $85,000; broadband; widebore; install included 젨� Varian Gemini 300: call for price; widebore; broadband; remote sampling arm; ~1992 젨� Varian Gemini 300: $69,000; proton/carbon probe; freight, install, 90-day warr. included 젨� Varian Unity 500: call for price; broadband Also available: Hitachi 570 scanning electron microscope, Kevex detector, running now, $35,000. BD FACSVantage and assorted FACScans Various other seqencers, synthesizers etc. are available; please inquire or check the website. Michael Sherrell Grizzly Analytical 707 887 2919/fax 707 887 9834 www.grizzlyanalytical.com From angela.hagan at kcl.ac.uk Wed May 26 06:20:47 1999 From: angela.hagan at kcl.ac.uk (Angela Hagan) Date: Mon Mar 7 05:41:18 2005 Subject: International Cancer Gene Therapy Conference Message-ID: <374BD90E.E5655DAD@kcl.ac.uk> The First Meeting of The International Society of Cancer Gene Therapy International Cancer Gene Therapy Conference London 8 - 9 July 1999 Speakers include: Michael Blaese (USA), Thomas Blankenstein (Germany), Reinder Bolhuis (Netherlands), Mario Columbo (Italy), David Curiel (USA), Olivier Danos (France), Manfred Dietel (Germany) Wafik El-Deiry (USA), Farzin Farzaneh (UK), Scott Freeman (USA), Phil Greenberg (USA), Nagy Habib (UK), Alan Irvine (UK), David Kirn (USA), Nick Lemoine (UK), Leif Lindholm (Sweden), Charles Link (USA), Michael Lotze (USA), Uwe M?ller (USA), Norman Nevin (UK), James Norris (USA), Jack Roth (USA), Prem Seth (USA), Kevin Scanlon (USA), Robert Sobol (USA), Jayne Spink (UK), Richard Vile (USA), Peter Walden (Germany), Mark Walport (UK). For further information see the following URL: http://www.kcl.ac.uk/kis/schools/kcsmd/molmed/conf.html -- Mrs Angela Hagan Department of Molecular Medicine Guy's, King's & St Thomas' School of Medicine Rayne Institute 123 Coldharbour Lane London SE5 9NU Tel: +44-171-848-5901 Fax: +44-171-733-3877 Email: angela.hagan@kcl.ac.uk Web: http://www.kcl.ac.uk/kis/schools/kcsmd/molmed/top.html From andrey at immunology.wustl.edu Mon May 24 13:34:34 1999 From: andrey at immunology.wustl.edu (Andrey Shaw) Date: Mon Mar 7 05:41:19 2005 Subject: ABI 377 automated DNA sequencer for sale Message-ID: <37499B8F.325981E1@immunology.wustl.edu> i trying to sell an ABI 377. It is two years old and used less than twice a week. I looking to get $50,000 for it. It is still under service contract and is a 36 lane. I can have it upgraded to 64 but the price will have to go to $60,000. It would come with 3 or 4 sets o plates and combs. please contact me if you are interested. Andrey S. Shaw Department of Pathology Washington University School of Medicine (314) 362-6311 From ljsmith at flash.net Thu May 27 01:17:25 1999 From: ljsmith at flash.net (LJ Smith) Date: Mon Mar 7 05:41:19 2005 Subject: cell death Message-ID: I have a question (actually 2) that is probably so elementary it might make some of you laugh, but working the premise that there are no stupid questions....... here goes. Assuming that cells are "programmed" to undergo a certain number of divisions and then they die, how is it that we have a continuous amount of cells to replace the dead ones, such as with skin cells? Where do these "fresh" cells come from? If you start with a single cell that undergoes mitosis and you now have 2 cells, each with its own genome, did the repicated chromatids assort in such a manner that some of the original strands and some of the replicated strands end up in both cells? Does a renewable source of young cells arise from some type of stem cell? These are a couple of those questions that I have consistently tucked away to be contemplated at a later date. Thanks, L.J. Smith From b9stst at rz.uni-jena.de Wed May 26 16:31:25 1999 From: b9stst at rz.uni-jena.de (Stefka Stoyanova) Date: Mon Mar 7 05:41:19 2005 Subject: Who sells NMDA receptor and nAch receptor? Message-ID: <7iioqk$aa6$1@fsuj19.rz.uni-jena.de> We are looking for purified or recombinant receptors- NMDA (glutamate) receptor and nicotinic acetylcholin receptor. If you know the right company or research group - please, contakt to me. S. From toni10 at ibv.csic.es Thu May 27 09:23:59 1999 From: toni10 at ibv.csic.es (Toni) Date: Mon Mar 7 05:41:19 2005 Subject: Macrophage culture from bone marrow Message-ID: <374D557F.4C302576@ibv.csic.es> Some one have a protocol about culture of monocyte/macrophage from bone marrow?. I will be very gratefull if you send me it. Thank you. From dneece at uiuc.edu Thu May 27 11:47:52 1999 From: dneece at uiuc.edu (Dave Neece) Date: Mon Mar 7 05:41:19 2005 Subject: Where can I buy Roccal? Message-ID: Who sells Roccal for cell culture use? Thanks, Dave. From haejin at netinfo.ubc.caX Thu May 27 13:04:28 1999 From: haejin at netinfo.ubc.caX (Austin So (Hae Jin)) Date: Mon Mar 7 05:41:19 2005 Subject: cell death References: Message-ID: <374D892B.1385898B@netinfo.ubc.caX> LJ Smith wrote: > Assuming that cells are "programmed" to undergo a certain number of divisions > and then they die, how is it that we have a continuous amount of cells to > replace the dead ones, such as with skin cells? Where do these "fresh" cells > come from? Cells are believed to be able to undergo 40-50 rounds of mitosis...so that's 2 exp(n)...that's *a lot* of cells that can be generated from one cell. This is determined by the size of chromatid ends called telomeres. But only a subset continue through mitosis and some can be considered as in a state of "quiescence"....otherwise you'd just be a mass of tissue. That's why you have a "renewable" cell population. Also, stem cells are believed to have an enzyme called telomerase that is active only in the stem cell population and not the differentiated cell population that maintains the size of these telomeres...preventing what is referred to as the end-replication problem. There is a recent article on the cloned sheep "Dolly" that is related to this...you might want to have a good read. > If you start with a single cell that undergoes mitosis and you now have 2 > cells, each with its own genome, did the repicated chromatids assort in such a > manner that some of the original strands and some of the replicated strands > end up in both cells? DNA has two strands. Each one of those strands acts as a template for replication, resulting in two "daughter" DNA molecules, which are segregated into the 2 (i.e. daughter) cells. So each daughter cell has a strand from its parents and the complementary strand is replicated. However, and theoretically, in the "nth" generation, only two of the cells in that nth generation will have the original "founding" DNA content... -- --- Austin P. So (Hae Jin) I.I.S.G.P. Biotechnology Laboratory University of British Columbia E-mail: haejin@netinfo.ubc.ca http://www.interchange.ubc.ca/neurosci/StudentPages/so.html (old) From deborahwilkinson at sprynet.com Thu May 27 15:54:53 1999 From: deborahwilkinson at sprynet.com (Deborah Wilkinson) Date: Mon Mar 7 05:41:19 2005 Subject: inclusion bodies, very basic question Message-ID: <7ik4s6$jps$1@juliana.sprynet.com> Are "inclusion bodies" exclusively associated with expression of hydrophobic proteins in living cells? In other words, can "inclusion bodies" per se form when using an in vitro expression system? I am asking this because I a vendor of in vitro expression systems indicated that one advantage of such systems can be the avoidance of inclusion bodies. However, the proteins prone to this are still hydrophobic, so one might expect to have some solubility problems, regardless. Do "inclusion bodies" contain proteins or other molecules in addition to the one that is overexpressed? Thanks deborahwilkinson@sprynet.com From mdonken at artsci.wustl.edu Thu May 27 15:01:41 1999 From: mdonken at artsci.wustl.edu (Mike Onken) Date: Mon Mar 7 05:41:19 2005 Subject: cell death References: <374D892B.1385898B@netinfo.ubc.caX> Message-ID: <374DA4A3.1A6A1149@artsci.wustl.edu> "Austin So (Hae Jin)" wrote: > LJ Smith wrote: > > > Assuming that cells are "programmed" to undergo a certain number of divisions > > and then they die, how is it that we have a continuous amount of cells to > > replace the dead ones, such as with skin cells? Where do these "fresh" cells > > come from? > > Cells are believed to be able to undergo 40-50 rounds of mitosis... ??? Where did that number come from??? Basal Keratinocytes undergo mitosis once every day or two. So on average, assuming a human lifetime around 70 years, skin cells undergo 25,000 rounds of mitosis. The same is true of epithelial cells throughout the body. The problem of telomere shortening only appears when these cells are cultured away from their native stroma. -- Michael Onken -. .-. .-. .-. . mdonken@artsci.wustl.edu Mol. Cell Biol. ||X|||\ /|||X|||\ /| URL: Washington Univ |/ \|||X|||/ \|||X|| http://madsci.wustl.edu/ St. Louis, MO ' `-' `-' `-' `- From haejin at netinfo.ubc.caX Thu May 27 16:36:57 1999 From: haejin at netinfo.ubc.caX (Austin So (Hae Jin)) Date: Mon Mar 7 05:41:19 2005 Subject: cell death References: <374D892B.1385898B@netinfo.ubc.caX> <374DA4A3.1A6A1149@artsci.wustl.edu> Message-ID: <374DBAF8.E89CCE12@netinfo.ubc.caX> Mike Onken wrote: > "Austin So (Hae Jin)" wrote: > > > Cells are believed to be able to undergo 40-50 rounds of mitosis... > > ??? Where did that number come from??? Wasn't that the number that Hayflick got when he cultured fibroblasts? I thought it was somewhere around there... > Basal Keratinocytes undergo mitosis once every day or two. So on average, assuming a > human lifetime around 70 years, skin cells undergo 25,000 rounds of mitosis. Last thing I checked, our skin wasn't unicellular :)...i.e. how many rounds of cell division do you really think happens from the point it "leaves" the basal cell layer to the point it is dead? Certainly not 25,000...You are in effect saying that a single basal cell generates 2 exp(25,000) cells (!). Just try to calculate that number... > The > same is true of epithelial cells throughout the body. The problem of telomere > shortening only appears when these cells are cultured away from their native stroma. No...that's not entirely true...telomere shortening occurs after basal cells have undergone a basic level of differentiation but corresponding with telomerase inactivation. Though I will agree in that the "problem" of telomere shortening never has a chance of occuring because cells die way before that. -- --- Austin P. So (Hae Jin) I.I.S.G.P. Biotechnology Laboratory University of British Columbia E-mail: haejin@netinfo.ubc.ca http://www.interchange.ubc.ca/neurosci/StudentPages/so.html (old) From nitinsatav at HOTMAIL.COM Fri May 28 00:07:10 1999 From: nitinsatav at HOTMAIL.COM (Nitin Satav) Date: Mon Mar 7 05:41:19 2005 Subject: Employeement... Message-ID: <19990528050059.25152.qmail@hotmail.com> This is a multi-part message in MIME format. ------=_NextPart_000_6efab4fa_7d46896b$2f919057 Content-type: text/plain; format=flowed; Dear Sir, I have more then 3+ year Experince in PERL,CGI, ASP , JAVA, VB & ORACLE.If u find me sutable Please let me know. Waiting for your replay. 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Biochemist (Temporary Full-Time) - Montreal - 5/24/99 (filled) Project Manager - Montreal - 5/26/99 Analytical Team Leader - Montreal - 5/26/99 Information Specialist - Vancouver - 5/27/99 Scientist (Ph.D.) - Drug Design/Synthetic Chemistry -Vancouver - 5/27/99 Manager Investor/Media Relations - Vancouver - 5/27/99 Research Scientist, Organic Chemistry - Montreal - 5/27/99 Research Associates, Organic Chemistry - Montreal - 5/27/99 Research Scientists, Process and Development - Montreal -5/27/99 Research Scienctist, Cancer Biology - Montreal - 5/27/99 Scientific Recruiting Consultant at Cortex HR Inc. -Toronto - 5/28/99 Thanks, Michael Kalchman, Ph.D. From deborahwilkinson at sprynet.com Fri May 28 10:43:05 1999 From: deborahwilkinson at sprynet.com (Deborah Wilkinson) Date: Mon Mar 7 05:41:19 2005 Subject: in vitro translation to avoid inclusion bodies? Message-ID: <7im6vj$921$1@juliana.sprynet.com> If one has problems with inclusion bodies in an E. coli-based expression system, would it be sensible to try to express the same protein using an in vitro translation system? Thanks, deborahwilkinson@sprynet.com From sluwa at HOTBOT.COM Fri May 28 21:19:17 1999 From: sluwa at HOTBOT.COM (sluwa@HOTBOT.COM) Date: Mon Mar 7 05:41:19 2005 Subject: MLM with No Sponsoring & No Recruiting [xhxyi] Message-ID: <199905290206.KAA27729@mail-svr.ecotek.com.tw> Greetings, I would like to personally introduce you to a BRAND NEW International Opportunity being brought to the World by a seven year old mail order company specializing in herbal products. A company that will soon take the World by Storm! Traditional Chinese Herbs & Teas - MLM with No Sponsoring & No Recruiting They are the industries top promoters of Network Marketing Opportunities. There new and powerful system gives you the edge you need to succeed in the financial world. 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This method measures the DNA concentration of the cells. The advantage of measuring DNA concentration is that we can accurately measure: 1. the viable cells in the growth system 2. the actual division rate of the cells 3. determine its growth kinetics very accurately Can anyone out there please send me the detailed procedure to carry out this method if you have come across in any literature or references. My e-mail address is aloyraj@tm.net.my Thank you in advance. Aloysius Raj From rodrigue at CORREIO.FM.UL.PT Sun May 30 11:44:29 1999 From: rodrigue at CORREIO.FM.UL.PT (=?iso-8859-1?Q?Jo=E3o?= Rodrigues) Date: Mon Mar 7 05:41:19 2005 Subject: Live cell confocal microscopy Message-ID: <375177D1.E3CE35DD@correio.fm.ul.pt> Hi everybody, I need to do confocal fluorescence microscopy of live animal cells and I would appreciate if you could give me some help about choosing the best system to do it. 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We are not on the Internet to burden you with unsolicited advertising, and if we offended you in any way we do sincerely apologize. To removed from our list please email us back with "Remove" in the subject heading. Do you have the desire to make $2000 to $5000 PLUS PER WEEK, beginning almost immediately? How does $10,000 TO $25,000 PLUS PER MONTH, and a realistic STRONG SIX FIGURES this year sound? And, how about generating all of this by just sharing information? If you can bring desire and an honest effort to the table, we can show you how to NEVER WORRY ABOUT MONEY AGAIN! Opportunity is truly knocking! To find out more, call Toll Free. 1-800-636-6773 ext. 3886 God bless From Quirien at dds.nl Mon May 31 07:13:03 1999 From: Quirien at dds.nl (Quirien boone) Date: Mon Mar 7 05:41:19 2005 Subject: VP16 antibody Message-ID: <37527CCE.279FB144@dds.nl> Dear readers, I am a PhD student working in the plant field. I use a GAL4-VP16 transactivation system for regulating gene expression. 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