From fesquet at crbm.cnrs-mop.fr Tue Aug 1 06:44:51 2000 From: fesquet at crbm.cnrs-mop.fr (D. Fesquet) Date: Mon Mar 7 05:46:29 2005 Subject: vital dye for DNA Message-ID: <3986C5F4.D843106@crbm.cnrs-mop.fr> hi, guys, does anybody know any vital Dye for DNA to be used for cellular imaging with time lapse microscopy any help wellcome didier --- From nicholas_theodorakis at urmc.rochester.edu Tue Aug 1 08:02:24 2000 From: nicholas_theodorakis at urmc.rochester.edu (Nick Theodorakis) Date: Mon Mar 7 05:46:29 2005 Subject: vital dye for DNA References: <3986C5F4.D843106@crbm.cnrs-mop.fr> Message-ID: <0037f9a1.789cb531@usw-ex0106-046.remarq.com> fesquet@crbm.cnrs-mop.fr ("D. Fesquet") wrote: >hi, guys, > > >does anybody know any vital Dye for DNA to be used for cellular imaging >with time lapse microscopy > > >any help wellcome > > >didier > > Look at the Molecualr Probes website: http://www.probes.com/ In particular, you should take a look at their online handbook: http://www.probes.com/handbook/sections/0000.html Their is a section on staining nucleic acids in both living and fixed cells. Nick -- Nick Theodorakis nicholas_theodorakis@urmc.rochester.edu (I have no control over any advertisements below the dotted line.) ----------------------------------------------------------- Got questions? Get answers over the phone at Keen.com. Up to 100 minutes free! http://www.keen.com From rcjohnsen at aol.com Wed Aug 2 01:33:07 2000 From: rcjohnsen at aol.com (Rcjohnsen) Date: Mon Mar 7 05:46:29 2005 Subject: Embryonic Lens Prompts Eye Development Message-ID: <20000802023307.12858.00000013@ng-ck1.aol.com> DEVELOPMENTAL BIOLOGY: Embryonic Lens Prompts Eye Development Elizabeth Pennisi Sci 289:531 7-28-00 A blind cave fish is providing new insight into how eyes come to be. In work reported on page 631, developmental biologists Yoshiyuki Yamamoto and William Jeffery of the University of Maryland, College Park, show that the lens plays a leading role in eye development in this fish. If it doesn't form properly, the researchers found, the embryo will not go on to make the cornea and other eye structures. During the 1960s, work in Russia and Spain had suggested such a role for the lens, but this new study "nails it," says Peter Mathers, a developmental biologist at West Virginia University School of Medicine in Morgantown. What's more, he adds, because the eye develops similarly in all vertebrates, including humans, "the implications are much broader than [for] just the cave fish." The Maryland team has been studying the fish, which is called Astyanax mexicanus, for the past 6 years. Several dozen isolated populations of the species exist in northeastern Mexico, with some living in surface ponds and streams and others in caves and underground waterways. Over the past million years or so, the eyes of the underground fish have degenerated to varying degrees, while the surface fish have retained their large eyes. To begin to understand this difference, Jeffery and Yamamoto first monitored eye development in the blind fish. They observed a precursor lens and the rudiments of the optic cup forming during the embryo's first 24 hours. But soon afterward, they found, the cells in the embryonic lens underwent programmed cell death. Other eye structures, such as the cornea and the iris, never appeared, and the retina never developed distinct, organized layers, as it does in normal eyes. The eyeball gradually sank back into the socket and was covered by a flap of skin. Because eye development seemed to progress normally until the lens degenerated, Jeffery and Yamamoto wondered whether this disintegration was triggered by a signal from the embryo or from the lens itself. To find out, Yamamoto removed the embryonic lens from one eye of a blind cave fish embryo and replaced it with a lens from a surface fish embryo. He also did the opposite experiment, replacing the lens of an embryonic surface fish with one from a cave fish embryo. In all cases, he labeled the transplanted tissue with dye so he could track what happened to it. "It's not a complicated experiment, but it really [was] very elucidative," says Mathers. In both types of transplants, the lens behaved as if it were still in its original embryo. The one from the cave fish degenerated, even though it was in an environment conducive to further development, whereas the lens from the surface fish thrived in the cave fish embryo and the eye differentiated, forming a cornea, anterior chamber, and iris. These results show that "the lens plays a central role" in determining whether the eye develops, comments David Beebe, a developmental biologist at Washington University School of Medicine in St. Louis. Jeffery doesn't know, however, whether the fish can actually see, as a vision test is quite difficult to devise. Other recent work by Jeffery and his colleagues may explain why the lens undergoes programmed cell death in the cave fish. The researchers looked at early embryos for changes in the expression of a variety of proteins that help specify how cells differentiate into specific organs and tissues. As they reported last month in Boulder, Colorado, at the annual meeting of the Society for Developmental Biology, cave fish embryos seem to make more of a protein called Sonic hedgehog in the area destined to be the head. As a result, fewer cells are set aside to form the eyes (Science, 23 June, p. 2119). Jeffery suspects that with fewer cells to start with, the precursor lens may wind up smaller than usual, perhaps too small to survive, and therefore decays. "It's possible you are looking at a single gene defect that has caused a drastic developmental change," Mathers notes. Still unclear, however, is how the embryonic lens of the sighted surface fish triggers further eye development. Presumably the lens produces a molecular signal, which Jeffery and his colleagues hope to identify eventually. They also hope to pinpoint the genes involved in eye development in A. mexicanus. Studying different populations of the fish may provide clues to these genes, notes Beebe: Because populations became isolated when the fish could see and became blind independently, different mutations may be involved in each population. Summary of this Article Related articles in Science Similar articles found in: SCIENCE Online Search Medline for articles by: Pennisi, E. Alert me when: new articles cite this article Download to Citation Manager Collections under which this article appears: Development Related articles in Science: Central Role for the Lens in Cave Fish Eye Degeneration. Yoshiyuki Yamamoto and William R. Jeffery Science 2000 289: 631-633. (in Reports) [Abstract] [Full Text] Volume 289, Number 5479, Issue of 28 Jul 2000, pp. 522-523. Copyright ? 2000 by The American Association for the Advancement of Science. From bokcmho at ust.hk Wed Aug 2 02:50:04 2000 From: bokcmho at ust.hk (Maurice Ho) Date: Mon Mar 7 05:46:29 2005 Subject: Chemokine receptor antibody Message-ID: <3987D2AC.27D11FE5@ust.hk> Dear all, Does anyone can kindly suggest some sources for obtaining antisera against chemokine receptors? Maurice From cates at cc.umanitoba.ca Wed Aug 2 08:21:05 2000 From: cates at cc.umanitoba.ca (Don Cates) Date: Mon Mar 7 05:46:29 2005 Subject: Help! neuronal cell culture problem Message-ID: <39881d93.49487258@news.cc.umanitoba.ca> I've been doing cell culture of dissociated neurons from fetal rat medulla for several years. Recently the wheels have fallen off. I use 35 mm plastic dishes with a collagen (Vitrogen) coating. I then plate with neuron free medullary background cells for a week or more, then plate with my dissociated cells (mince with scissors, treat with trypsin for 15-20 min, gently triturate). The last few times I have done this, the cells seem to lift off most of the dish and form long, thick 'ropes' of cells which include the collagen and background cells. I've replaced my various media and components with fresh material with no luck. It is not invariable, I get the odd good dish. Can anyone suggest anything? Thanks Don Cates cates@cc.umanitoba.ca From anfortin at webnet.qc.ca Wed Aug 2 18:39:42 2000 From: anfortin at webnet.qc.ca (Tina) Date: Mon Mar 7 05:46:29 2005 Subject: confocal microscopy Message-ID: <2j2i5.1183$WM6.88326771@news1.mtl.metronet.ca> Is there any course and/or formation on confocal microscopy, like intensive theorical and practical course where I could go? Any info? Thanks a lot Tina From pd1 at mole.bio.cam.ac.uk Thu Aug 3 10:16:15 2000 From: pd1 at mole.bio.cam.ac.uk (Paul) Date: Mon Mar 7 05:46:29 2005 Subject: confocal microscopy References: <2j2i5.1183$WM6.88326771@news1.mtl.metronet.ca> Message-ID: <39898CBF.D58CE701@mole.bio.cam.ac.uk> Tina wrote: > Is there any course and/or formation on confocal microscopy, like intensive > theorical and practical course where I could go? > I think I got a flyer from Perkin Elmer (IIRC, seems a strange company for confocal stuff...) a while ago advertising such a course. Otherwise, try BioRad or Leica websites. Paul From eric at scientificresources.com Thu Aug 3 10:56:48 2000 From: eric at scientificresources.com (eric celidonio) Date: Mon Mar 7 05:46:29 2005 Subject: US-MA-Bedford-Project Scientist, Molecular Biology Message-ID: ------------------------------------------------------------------------ Initiating, directing, executing R&D projects in the areas of genomics, nucleic acid arrays, protein microarrays, high throughput molecular biology assays in collaboration with research staff and individual studies. Creates, adheres to project plans, meeting resources, budgets, time estimates. Generates ideas for project areas, designs experiments, analyzes data and interprets results. Presents results to colleagues, teams, management. Investigates feasibility of applying scientific principles and concepts to potential inventions, products, and technologies. May publish and/or submit patent applications. Works on problems of diverse scope and normally receives no instructions on routine work, general instruction on new assignments. Qualifications: Ph.D. in Life science discipline, preferably Molecular Biology, Biochemistry, Genetics with 3+ years experience. MS with 5+ years experience. Industry experience with 2+ years in development and use of DNA microarrays as tools for research and/or drug discovery highly desirable. Demonstrated ability in 1) designing and executing experiments utilizing state-of-the-art molecular biology technology in the areas of nucleic acid labeling, analysis and quantitation; 2) Characterization/development of protein technologies and novel assay systems for molecular biology applications. Expert knowledge of current scientific literature and state-of-the-art technologies, theories, principles, patents in own area of scientific discipline. Knowledge of statistical principles of experimental design and analysis for evaluating precision and accuracy; understanding of experimental designs for multivariate optimization and analysis. Please email Eric@scientificresources.com to forward resume or for more information. All replies will be held confidential. From serotone at replies_to_newsgroup.com Thu Aug 3 13:50:35 2000 From: serotone at replies_to_newsgroup.com (Serotone) Date: Mon Mar 7 05:46:29 2005 Subject: Help! neuronal cell culture problem References: <39881d93.49487258@news.cc.umanitoba.ca> Message-ID: "Don Cates" wrote in message news:39881d93.49487258@news.cc.umanitoba.ca... > I've been doing cell culture of dissociated neurons from fetal rat > medulla for several years. Recently the wheels have fallen off. > I use 35 mm plastic dishes with a collagen (Vitrogen) coating. I then > plate with neuron free medullary background cells for a week or more, > then plate with my dissociated cells (mince with scissors, treat with > trypsin for 15-20 min, gently triturate). The last few times I have done > this, the cells seem to lift off most of the dish and form long, thick > 'ropes' of cells which include the collagen and background cells. I've > replaced my various media and components with fresh material with no > luck. It is not invariable, I get the odd good dish. > Can anyone suggest anything? Do these ropes consist of lysed cells or healthy ones? Trypsin activity remaining in the cells suspension would cause this..... -- serotonin1 at hushmail dot com ICQ# 6819163 PLUR From cates at cc.umanitoba.ca Thu Aug 3 14:39:54 2000 From: cates at cc.umanitoba.ca (Don Cates) Date: Mon Mar 7 05:46:30 2005 Subject: Help! neuronal cell culture problem References: <39881d93.49487258@news.cc.umanitoba.ca> Message-ID: <3989c895.16802630@news.cc.umanitoba.ca> On Thu, 3 Aug 2000 13:50:35 -0500, "Serotone" wrote: >"Don Cates" wrote in message >news:39881d93.49487258@news.cc.umanitoba.ca... >> I've been doing cell culture of dissociated neurons from fetal rat >> medulla for several years. Recently the wheels have fallen off. >> I use 35 mm plastic dishes with a collagen (Vitrogen) coating. I then >> plate with neuron free medullary background cells for a week or more, >> then plate with my dissociated cells (mince with scissors, treat with >> trypsin for 15-20 min, gently triturate). The last few times I have done >> this, the cells seem to lift off most of the dish and form long, thick >> 'ropes' of cells which include the collagen and background cells. I've >> replaced my various media and components with fresh material with no >> luck. It is not invariable, I get the odd good dish. >> Can anyone suggest anything? > >Do these ropes consist of lysed cells or healthy ones? Trypsin activity >remaining in the cells suspension would cause this..... Healthy cells. I thought of that early but since the 4ml of 0.125 mg/ml trypsin is passed through 10 ml of MEM with 5% FBS I thought that it would be inactivated. But that was a while ago and nothing else has worked so I'll check it again more thoughly. -------- Oh my, I feel dumb! I just rechecked and the stock trypsin that I got from a new supplier is 10x the concentration that I was using before. So I have been using 1.25 mg/ml trypsin. That might be it. Thanks for the prod to go back over the obvious. Don Cates cates@cc.umanitoba.ca From CDrouet at chu-grenoble.fr Fri Aug 4 04:38:34 2000 From: CDrouet at chu-grenoble.fr (Drouet, Christian) Date: Mon Mar 7 05:46:30 2005 Subject: anti-puromycin Ab Message-ID: <51F46819EB1FD211888200A0C94CCDDB0F03E2@EXPNT08> Dear Colleages, Is there anyone who know where could be purchased the anti-puromycin Ab to perform immunoprecipitation experiments? I would be happy to get any response. Please reply to CDrouet@chu-grenoble.fr Thanks a lot Christian DROUET --- From nessa at parksrule.net Fri Aug 4 06:33:43 2000 From: nessa at parksrule.net (nessa@parksrule.net) Date: Mon Mar 7 05:46:30 2005 Subject: Would You like to Quit Your Job ? Message-ID: I Have To Get This Off My Chest Before I Explode! Dear Friend, You can earn $50,000 or more in the next 90 days sending e-mail. Seem impossible? Is there a catch? Well it is Possible and NO, there is not a catch! Read on for your way to financial freedom. THIS IS A LEGITIMATE, LEGAL, MONEY MAKING OPPORTUNITY. It does not require you to come into contact with people, do any hard work, and best of all, you never have to leave the house except to get the mail. If you believe that someday you'll get that big break that you've been waiting for, THIS IS IT! Simply follow the instructions, and your dreams will come true. ************************************************************* 1. Order all 4 reports shown on the list below. For each report, send $5.00 CASH, the NAME & NUMBER OF THE REPORT YOU ARE ORDERING, YOUR E-MAIL ADDRESS, YOUR NAME & PHYSICAL ADDRESS. 2. After you've ordered the four reports. Take THIS advertisement/email and remove the name and address under REPORT #4. Insert your name/address in the REPORT #1 position and move everyone else down a spot. The #4 person has now completed the cycle and is counting a mountain of $5 bills. Your cost to participate in this is practically nothing! (surely you can afford $20). You obviously already have an Internet Connection and e-mail is FREE! There are two primary methods of building your downline: METHOD #1: SENDING BULK E-MAIL METHOD #2 - PLACING FREE ADS ON THE INTERNET AND NEWSPAPERS Follow this example to achieve the STAGGERING results below: 1st level-your 10 members with $5 .......................$50 2nd level-10 members from those 10 ($5 x 100)...........$500 3rd level-10 members from those 100 ($5 x 1,000)......$5,000 4th level-10 members from those 1,000 ($5 x 10,000)..$50,000 TOTAL -----------------------------------------------$55,550 For every $5.00 you receive, all you must do is e-mail them the report they ordered. THAT'S IT! ---------------------------------------- THE REPORTS ---------------------------------------- *** Order Each REPORT by NAME and NUMBER *** Notes: ALWAYS SEND $5 CASH (U.S. CURRENCY) FOR EACH REPORT. CHECKS NOT ACCEPTED - NO CHECKS - $5 BILLS ONLY Make sure the cash is concealed by wrapping it in at least one sheet of paper. On one of those sheets of paper, include: (a) the name & number of the report you are ordering, (b) your e-mail address, and (c) your name & postal address. PLACE YOUR ORDER FOR THESE REPORTS NOW! -------------------------------------- REPORT #1: "HOW TO SEND OUT 1 MILLION E-MAILS FOR FREE!" ORDER REPORT #1 FROM: Ruby Carr 3710 Belle Avenue Baltimore, MD 21215 -------------------------------------------------- REPORT #2: "HOW TO BECOME A MILLIONAIRE UTILIZING THE POWER OF MULTILEVEL MARKETING AND THE INTERNET!" ORDER REPORT #2 FROM: Bruce Miller 4680 Bryant Road Buford, GA.30518 ----------------------------------------------------- REPORT #3: "HOW TO CREATE AN IRRESISTIBLE INFORMATION PRODUCT!" ORDER REPORT #3 FROM: DNW Enterprises P.O. Box 190475 Burtonm MI.48519 ----------------------------------------------------- REPORT #4: "SEVEN STEPS TO ONLINE MARKETING SUCCESS!" ORDER REPORT #4 FROM: JKA 6634 NW Sioux Dr. Kansa City, MO. 64152 ----------------------------------------------------- That's it! My personal testimony is that it works. I had a bulk email company send a minimal amount and received several times my investment. I don't see myself stop mailing this program ANYTIME soon! Sincerely, Allison Primner ******************************************************** This message is sent in compliance of the proposed bill:SECTION 301. Per Section 301, Paragraph (a)(2)(C) of S. 1618, further transmissions to you by the sender of this email may be stopped at no cost to you by sending a reply to this email address with the word remove in the subject line. This message is not intended for residents in the State of Washington, screening of addresses has been done to the best of our technical ability. If you are a Washington, Virginia, or California resident or otherwise wish to be removed from this list, further transmissions to you by the sender of this email may be stopped at no cost to you by sending a reply to mstrsrvcs@mailme.org with the word remove in the subject line. ********************************************************* 7-s --- From love at 163.net Sat Aug 5 00:07:46 2000 From: love at 163.net (ÐÂÀÅ) Date: Mon Mar 7 05:46:30 2005 Subject: ÐÂÀÅËÍ´óÀñÁË! Message-ID: <200008051307375.SM00363@xingming> Skipped content of type multipart/alternative From list at physlink.com Sat Aug 5 08:55:22 2000 From: list at physlink.com (Anton Skorucak) Date: Mon Mar 7 05:46:30 2005 Subject: Physics and Astronomy ... Message-ID: <258182000865135547590@physlink.com> "One thing I have learned in a long life: that all our science, measured against reality, is primitive and childlike - and yet it is the most precious thing we have." - Albert Einstein Are you interested in Physics, Astronomy and Science in general? Then check out Einstein's favorite web site: http://www.physlink.com Here are just some of the things you will find there: - General science reference: Glossary, Physical Constants, Unit Conversion, Astro Constants, Nuclear/Particle Data, Periodic Tables, Materials Safety, Exact Time, Equations, Educator Reference http://www.physlink.com/reference.cfm - FREE weekly physics and astronomy Newsletter http://www.physlink.com/newsletter/signup.cfm - Latest news stories updated daily http://www.physlink.com - Jobs Board: browse and post job ads for physicists, astronomers, mathematicians and engineers. http://www.physlink.com/jobs.cfm - FREE membership in the Physics Teachers Club: get free books, lesson plans, meet other teachers, etc. http://www.physlink.com/teacher_reg.cfm - Ask the Experts: you can send in your physics questions and experts around the world will answer! http://www.physlink.com/ask_experts.cfm - Astronomy links: the most comprehensive source of top astronomy web sites: http://www.physlink.com/astronomy.cfm -Physics and Astronomy Departments Worldwide: comprehensive listing http://www.physlink.com/departments.cfm - FREE science software trial versions, reviews, etc. http://www.physlink.com/software.cfm - Physics FUN: jokes, cartoons, virtual Einstein cards and more ... http://www.physlink.com/fun.cfm ... and much, much more at: http://www.physlink.com ----------------------------------------------------------- If you wish to remove your e-mail address from any future mailings, just click this link: http://www.physlink.com/newsletter/removeoptin.cfm?Email=cellbiol@net.bio.ne t (or copy and paste the above link into your browser and hit enter) You were referred to us as someone who is interested in science - if this is not true please accept our apologies for this e-mail. DO NOT REPLY to this e-mail - no human will process the replies - use the above instructions to remove your e-mail from future mailings. Best Regards! --- From mail at msn.com Mon Aug 7 01:15:20 2000 From: mail at msn.com (mail@msn.com) Date: Mon Mar 7 05:46:30 2005 Subject: Come here to win a new cadillac ! -siydphk Message-ID: -------------- next part -------------- An HTML attachment was scrubbed... URL: http://iubio.bio.indiana.edu/bionet/mm/cellbiol/attachments/20000807/4f0c33ca/attachment.html From autoride at chin.com Mon Aug 7 10:14:08 2000 From: autoride at chin.com (autoride@chin.com) Date: Mon Mar 7 05:46:30 2005 Subject: Best Prices On New Cars!! Message-ID: <200008071510.IAA15250@ecis.ecis.com> Get A Great Price On A New Car! Absolutely Free! Want to save time and money? Want to have quick access to car quotes? Want to have all makes and models available to you? If you answered yes to any of these, then take advanage of this free, no-hassle service. Simply click on the link below to get low prices on all makes and models of new and used cars, without having to negotiate with a dealer. It's painless and stress-free! GO TO http://www.huesandbubbles.com ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ If for any reason you do not want to recieve offers in the future click the link below to be removed mailto:naymas@china.com?subject=Remove --- From urbane45 at netscape.net Mon Aug 7 17:31:33 2000 From: urbane45 at netscape.net (urbane45) Date: Mon Mar 7 05:46:30 2005 Subject: Enzyme expression Message-ID: <20000807223129.28880.qmail@ww190.netaddress.usa.net> Hello, I am trying to locate enzymes that have difficulty in expressing in bacteria or yeast strains, but express well in mammalian cells. Any help would be most welcome. urbane ____________________________________________________________________ Get your own FREE, personal Netscape WebMail account today at http://webmail.netscape.com. --- From scientists_2001 at yahoo.com Mon Aug 7 21:49:39 2000 From: scientists_2001 at yahoo.com (Kevin Stazey) Date: Mon Mar 7 05:46:30 2005 Subject: International Membership Encouraged Message-ID: <20000808024930.25391.qmail@web6102.mail.yahoo.com> The Society for Molecular and Cell Biology is open for international membership. No geographical exclusivity. Everyone is invited as long as she/he has the minimum requirements. Applications are highly welcomed from new graduates with a Master's Degree in Molecular Biology/ and Cell Biology. For those whose master's are in the related field (Biology, Microbiology etc.) or those who have finished a medical course, they must either have a master's thesis in the area of molecular and cell biology or have undergone considerable training in mol. and cell bio. in a reputable laboratory or have participated in research(es) in the fields of molecular and cell biology from a reputable institution or have taken considerable advanced courses in molecular and cell biology and actively teaching such course(s) in higher education. Letters of recommendation are encouraged from superiors. If interested, kindly send a note to Scientists_2001@yahoo.com Thanks. __________________________________________________ Do You Yahoo!? Kick off your party with Yahoo! Invites. http://invites.yahoo.com/ --- From e.e.morrisonNOe.SPAM at leeds.ac.uk.invalid Tue Aug 8 02:33:08 2000 From: e.e.morrisonNOe.SPAM at leeds.ac.uk.invalid (Ewan) Date: Mon Mar 7 05:46:30 2005 Subject: Alexa Fluor fluorophores References: <0cafcffc.1f8cd3b8@usw-ex0106-046.remarq.com> Message-ID: <0762bc3a.deaf152b@usw-ex0104-032.remarq.com> Hey Nick. I can wholeheartedly recommend the Alexa reagents. They are indeed very photostable and brighter than FITC etc. For multicolor work the preabsorbed antibodies are superb; I've mixed mouse and rat primaries and got no cross-talk at all. They look a little expensive at first sight but you can use them at a much lower concentration so they actually represent good value. Spin them hard before use. We use 488, 568 and a little 594 and get good results with all. Good luck, Ewan Morrison. ----------------------------------------------------------- Got questions? Get answers over the phone at Keen.com. Up to 100 minutes free! http://www.keen.com From spfeiff at nimr.mrc.ac.uk Tue Aug 8 05:56:10 2000 From: spfeiff at nimr.mrc.ac.uk (Sven Pfeiffer) Date: Mon Mar 7 05:46:30 2005 Subject: Depolymerisation of MT and Actin Cytoskeleton Message-ID: <200008081057.LAA02209@ns4.local.nimr> Hi there, I am looking for membrane permeable reagents that depolymerise MT and/or actin cytoskeleton. Any suggestions are greatly appreciated. Cheers Sven --------------------------------------------------- Medical Research Council National Institute of Medical Research Division of Mammalian Development The Ridgeway Mill Hill London NW7 1AA UK Tel 44 (0)208 959-3666 x2017 Fax 44 (0)208 913-8543 e-mail: spfeiff@nimr.mrc.ac.uk --- From diagaid at vsnl.com Tue Aug 8 06:08:04 2000 From: diagaid at vsnl.com (DPSB) Date: Mon Mar 7 05:46:31 2005 Subject: www.DnaMap.com Message-ID: <398FEA14.BD91D710@vsnl.com> Hello, Dna Map of Man has been decoded. It is a great step for Mankind. Genome related discoveries are going to take place at a fast pace. It is very important to have your own website at a powerful but easy to remember address. You can have your own website at any of the following prestigious addresses :-- DnaMap.com Dna123.com GenesInc.com GeneticLab.com , .net and .org ViralMarkers.com GeneticEngineer.com BioMedEngineering.com Even if you already have a website, you can link your website from these names to increase traffic to your existing website. If you are interested in any of these domain names or need additional information, please contact me . Best of Regards, DPSB http://www.DnaMap.com -------------- next part -------------- An HTML attachment was scrubbed... URL: http://iubio.bio.indiana.edu/bionet/mm/cellbiol/attachments/20000808/3c8c2358/attachment.html From leo28 at duke.edu Tue Aug 8 07:14:42 2000 From: leo28 at duke.edu (Leonardo Serrano) Date: Mon Mar 7 05:46:31 2005 Subject: lovastatin.. Message-ID: Hi everybody... We did purchase Lovastatin (Cat No. 438185) from Calbiochem. According to the technical data sheet, this product requieres activation for use in cell culture. Could you please be more especific on this matter, since we require to use it for that purpose. How can we activate this compound? Thanks in advance for attending our request. Waiting for your fast response, Leonardo Serrano & Peter Stüven From rh at mblab.gla.ac.uk Tue Aug 8 08:24:30 2000 From: rh at mblab.gla.ac.uk (Robert Hartley) Date: Mon Mar 7 05:46:31 2005 Subject: Depolymerisation of MT and Actin Cytoskeleton References: <200008081057.LAA02209@ns4.local.nimr> Message-ID: In article <200008081057.LAA02209@ns4.local.nimr>, spfeiff@nimr.mrc.ac.uk (Sven Pfeiffer) wrote: > Hi there, > > I am looking for membrane permeable reagents that depolymerise MT and/or > actin cytoskeleton. Any suggestions are greatly appreciated. For MicT's Colchicine, nocodazole > Cheers > > Sven Cheers Bob; Cloudy Scotland From serotone at replies_to_newsgroup.com Tue Aug 8 12:02:08 2000 From: serotone at replies_to_newsgroup.com (Serotone) Date: Mon Mar 7 05:46:31 2005 Subject: Help! neuronal cell culture problem References: <39881d93.49487258@news.cc.umanitoba.ca> <3989c895.16802630@news.cc.umanitoba.ca> Message-ID: > I have been using 1.25 mg/ml trypsin. That might be it. > > Thanks for the prod to go back over the obvious. No prob. Been there, done that. ;-) -- serotonin1 at hushmail dot com ICQ# 6819163 PLUR From f.Vaillant at vet.gla.ac.uk Wed Aug 9 03:47:32 2000 From: f.Vaillant at vet.gla.ac.uk (Francois) Date: Mon Mar 7 05:46:31 2005 Subject: cd4 expression Message-ID: <39911AA4.AFE332BB@vet.gla.ac.uk> Hi, Would there be a treatment that could be administred to mice (by ip injection) that could induce premature expression of CD4 at the DP stage (or CD8 immature SP, just before DP stage). Such a treatment would repress the silencing operating at hat stage. Thanks for your help. Francois From g.coulton at ic.ac.uk Wed Aug 9 07:23:33 2000 From: g.coulton at ic.ac.uk (Gary Coulton) Date: Mon Mar 7 05:46:31 2005 Subject: ICHC 2000 Cell Biology and Imaging Message-ID: <39914D45.96EEAEDF@ic.ac.uk> Hi Everyone, If you are making a last minute decision as to which conferences to attend this summer/fall then take a look at ICHC 2000 "Cell Biology and Imaging Tools for the New Century". 3-8th Sept. 2000 York, UK Take look at the fantastic Final Programme at http://www.med.ic.ac.uk/external/ichc_2000 Over 100 leading speakers in 35 symposia York is a most beautiful medieval walled city, a great place for a vacation. You can register for the week or if close to, York then come for the day! Gary Coulton Organiser ICHC 2000 From elongelongswb at elong.com Wed Aug 9 08:04:02 2000 From: elongelongswb at elong.com (www.001.com.cn) Date: Mon Mar 7 05:46:31 2005 Subject: ÄãºÃ Message-ID: <20000809022125.SM00283@bao> Skipped content of type multipart/alternative-------------- next part -------------- An HTML attachment was scrubbed... URL: http://iubio.bio.indiana.edu/bionet/mm/cellbiol/attachments/20000809/13e11713/001.htm From rcjohnsen at aol.com Wed Aug 9 16:12:06 2000 From: rcjohnsen at aol.com (Rcjohnsen) Date: Mon Mar 7 05:46:31 2005 Subject: Crystal Structure of Rhodopsin: Sci Aug. 4, 2000 Message-ID: <20000809171206.25265.00000137@ng-fz1.aol.com> Sci Aug. 4, 2000 Biochemistry Crystal Structure of Rhodopsin: A G Protein-Coupled Receptor Krzysztof Palczewski,1,2,3* Takashi Kumasaka,7 Tetsuya Hori,7,8 Craig A. Behnke,4,6 Hiroyuki Motoshima,7 Brian A. Fox,4,6 Isolde Le Trong,5,6 David C. Teller,4,6 Tetsuji Okada,1 Ronald E. Stenkamp,5,6* Masaki Yamamoto,7 Masashi Miyano7* Heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors (GPCRs) respond to a variety of different external stimuli and activate G proteins. GPCRs share many structural features, including a bundle of seven transmembrane ?helices connected by six loops of varying lengths. We determined the structure of rhodopsin from diffraction data extending to 2.8?angstroms resolution. The highly organized structure in the extracellular region, including a conserved disulfide bridge, forms a basis for the arrangement of the seven-helix transmembrane motif. The ground-state chromophore, 11-cis-retinal, holds the transmembrane region of the protein in the inactive conformation. Interactions of the chromophore with a cluster of key residues determine the wavelength of the maximum absorption. Changes in these interactions among rhodopsins facilitate color discrimination. Identification of a set of residues that mediate interactions between the transmembrane helices and the cytoplasmic surface, where G-protein activation occurs, also suggests a possible structural change upon photoactivation. 1 Department of Ophthalmology, 2 Department of Pharmacology, 3 Department of Chemistry, 4 Department of Biochemistry, 5 Department of Biological Structure, and 6 Biomolecular Structure Center, University of Washington, Seattle, WA 98195,?USA. 7 Structural Biophysics Laboratory, RIKEN Harima Institute, 1-1-1?Kouto, Mikazuki-cho, Sayo-gun, Hyogo 679-5148,?Japan. 8 Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259?Nagatsuta, Midori-ku, Yokohama 226-8501,?Japan *?? To whom correspondence should be addressed. E-mail: miyano@spring8.or.jp (M.M.); palczews@u.washington.edu (K.P.); stenkamp@u.washington.edu (R.E.S.). ------------------------------------------------------------------------ From rcjohnsen at aol.com Wed Aug 9 16:27:13 2000 From: rcjohnsen at aol.com (Rcjohnsen) Date: Mon Mar 7 05:46:31 2005 Subject: Pol : A DNA Polymerase Required for Sister Chromatid Cohesion Message-ID: <20000809172713.25265.00000149@ng-fz1.aol.com> Molecular Biology Sci Aug 4, 00 Pol : A DNA Polymerase Required for Sister Chromatid Cohesion Zhenghe Wang, Irene B. Casta?o,* Alejandro De Las Pe?as,* Carrie Adams, Michael F. Christman Establishment of cohesion between sister chromatids is coupled to replication fork passage through an unknown mechanism. Here we report that TRF4, an evolutionarily conserved gene necessary for chromosome segregation, encodes a DNA polymerase with -polymerase-like properties. A double mutant in the redundant homologs, TRF4 and TRF5, is unable to complete S phase, whereas a trf4 single mutant completes a presumably defective S phase that results in a failure of cohesion between the replicated sister chromatids. This suggests that TRFs are a key link in the coordination between DNA replication and sister chromatid cohesion. Trf4 and Trf5 represent the fourth class of essential nuclear DNA polymerases (designated DNA polymerase kappa) in Saccharomyces cerevisiae and probably in all eukaryotes. Department of Microbiology, University of Virginia, Box 441,?Jordan Hall, 1300?Jefferson Park Avenue, Charlottesville, VA 22908,?USA. *?? Present address: Johns Hopkins University, Department of Molecular Biology and Genetics, 725?North Wolfe Street, 504?PCTB, Baltimore, MD 21205-2185, USA. ?? To whom correspondence should be addressed. E-mail: mfc3f@virginia.edu ------------------------------------------------------------------------ From rcjohnsen at aol.com Wed Aug 9 16:38:51 2000 From: rcjohnsen at aol.com (Rcjohnsen) Date: Mon Mar 7 05:46:31 2005 Subject: Cell-Cell Signaling and Movement by the Floral Transcription Factors LEAFY and APETALA1 Message-ID: <20000809173851.25265.00000157@ng-fz1.aol.com> Botany Sci Aug 4, 00 Cell-Cell Signaling and Movement by the Floral Transcription Factors LEAFY and APETALA1 Allen Sessions,1, 2* Martin F. Yanofsky,1 Detlef Weigel2 LEAFY (LFY) and APETALA1 (AP1) encode unrelated transcription factors that activate overlapping sets of homeotic genes in Arabidopsis flowers. Sector analysis and targeted expression in transgenic plants were used to study whether LFY and AP1 can participate in cell-cell signaling between and within different layers of the floral meristem. LFY signaled equally well from all layers and had substantial long-range action within layers. Nonautonomous action of LFY was accompanied by movement of the protein to adjacent cells, where it directly activated homeotic target genes. In contrast, AP1 had only limited nonautonomous effects, apparently mediated by downstream genes because activation of early target genes by AP1 was cell-autonomous. 1 Department of Biology, University of California, San Diego, La Jolla, CA 92093,?USA. 2 Plant Biology Laboratory, Salk Institute for Biological Studies, 10010?North Torrey Pines Road, La Jolla, CA 92037,?USA. *?? Present address: Novartis Agricultural Discovery Institute, 3115?Merryfield Row, San Diego, CA 92121,?USA. ?? To whom correspondence should be addressed. E-mail: marty@ucsd.edu, weigel@salk.edu ------------------------------------------------------------------------ From rcb5 at msn.com Wed Aug 9 18:25:52 2000 From: rcb5 at msn.com (Ron Blue) Date: Mon Mar 7 05:46:31 2005 Subject: Help! neuronal cell culture problem References: <39881d93.49487258@news.cc.umanitoba.ca> Message-ID: <00c901c00259$01c4e640$5a1d183f@pavilion> While there was no hint in your discussion, I believe your cells were exposed to fluctuating electromagnetic fields that is a harmonic of 10 hertz. Perhaps, a florescent light, a fan near by, a cellar phone, a person near by with a satellite dish. Ron Blue http://turn.to/ai ----- Original Message ----- From: "Don Cates" To: ; Sent: Wednesday, August 02, 2000 9:21 AM Subject: Help! neuronal cell culture problem > I've been doing cell culture of dissociated neurons from fetal rat > medulla for several years. Recently the wheels have fallen off. > I use 35 mm plastic dishes with a collagen (Vitrogen) coating. I then > plate with neuron free medullary background cells for a week or more, > then plate with my dissociated cells (mince with scissors, treat with > trypsin for 15-20 min, gently triturate). The last few times I have done > this, the cells seem to lift off most of the dish and form long, thick > 'ropes' of cells which include the collagen and background cells. I've > replaced my various media and components with fresh material with no > luck. It is not invariable, I get the odd good dish. > Can anyone suggest anything? > > Thanks > Don Cates > cates@cc.umanitoba.ca > > > --- From mlggibson at earthlink.net Thu Aug 10 01:22:22 2000 From: mlggibson at earthlink.net (Marylou Gibson) Date: Mon Mar 7 05:46:32 2005 Subject: NEW Adenovirus Purification Kit Message-ID: A company in San Diego has introduced and has been marketing an adenovirus purification KIT. This kit is used is place of the routine cesium chloride density gradient method, and cuts the time of purification from days to ONE HOUR. Once you use it you will never go back. The kit utilizes membrane technology to purify virus and produces amounts of virus equivilent if not superior to the gradient derived virus. High titered infectious virus is easily obtained. An AAV and a baculovirus purification kit are in the pipline for release soon. Check out their website at www.virapur.com From cuthbert4187 at my-deja.com Thu Aug 10 05:47:55 2000 From: cuthbert4187 at my-deja.com (cuthbert4187@my-deja.com) Date: Mon Mar 7 05:46:32 2005 Subject: Petition at www.letspetition.com Message-ID: <8mu18q$bdm$1@nnrp1.deja.com> There is an anonymous petition running at www.letspetition.com that may be of interest to the readers of this newsgroup. Sent via Deja.com http://www.deja.com/ Before you buy. From matth at NOSPAM.biols.sussex.ac.uk Thu Aug 10 06:19:08 2000 From: matth at NOSPAM.biols.sussex.ac.uk (Matt Hicks) Date: Mon Mar 7 05:46:32 2005 Subject: Protease cleavage Message-ID: <8mu367$fs1$1@ames.central.susx.ac.uk> Hi everyone, Does anyone know of a program for predicting protease cleavage sites (and size of fragments?)? Failing that is there a database of proteases with their recognition sequences so that I can write my own programme to do this? Many thanks, Matt From rgrant at netscape.net Thu Aug 10 07:47:48 2000 From: rgrant at netscape.net (Richard P. Grant) Date: Mon Mar 7 05:46:32 2005 Subject: Protease cleavage References: <8mu367$fs1$1@ames.central.susx.ac.uk> Message-ID: In article <8mu367$fs1$1@ames.central.susx.ac.uk>, "Matt Hicks" wrote: > Hi everyone, > Does anyone know of a program for predicting protease cleavage sites (and > size of fragments?)? > Failing that is there a database of proteases with their recognition > sequences so that I can write my own programme to do this? GCG has peptidemap (IIRC) which does this. R -- Richard P. Grant MAD Phil http://www2.mrc-lmb.cam.ac.uk/personal/rpg/ Structural Studies http://www.scienceboard.net/ MRC-LMB Please reply to rpg 'at' mrc-lmb.cam.ac.uk From Andreas.Schatzlein at beatson.gla.ac.uk Thu Aug 10 12:02:32 2000 From: Andreas.Schatzlein at beatson.gla.ac.uk (Andreas Schatzlein) Date: Mon Mar 7 05:46:32 2005 Subject: UK Postdoc Message-ID: <8mun7p$m1v$1@singer.cent.gla.ac.uk> University of Glasgow CRC Department of Medical Oncology /Department of Veterinary Pathology Post-doctoral research fellowship Salary £18,731-20,465 We are seeking a Postdoctoral Fellow to join a research team investigating non-viral gene delivery systems. The project funded through a Sir Henry Wellcome Commemorative Award will examine novel strategies to increase the efficiency of non-viral vectors in particular it will focus on aspects of nuclear DNA transport. Experience in molecular biology/cell biology would be an advantage. The most important requisites are enthusiasm and initiative. The Departments are well-equipped modern research laboratories housing more than thirty groups from various disciplines with a rich academic life. The appointment will be for 18 months in the first instance and is available from the 1st of October 2000. Informal enquiries may be made to Dr Andreas Schätzlein (e-mail: aschaetz@udcf.gla.ac.uk, (0)141 330 4354) or Dr Kin-Chow Chang (e-mail: k.chang@vet.gla.ac.uk, (0)141 330 4123). Applications, enclosing a current CV and the contact details of two professional or academic referees, should be sent to Mrs Margaret Jenkins, CRC Department of Medical Oncology, Garscube Estate, Glasgow G61 1BD, quoting ref. PDAS0700. Tel.: (0)141 330 4125, Fax: (0)141 330 4127, e-mail: M.Jenkins@beatson.gla.ac.uk From jbaluch at home.com Thu Aug 10 16:39:26 2000 From: jbaluch at home.com (Jeff Baluch) Date: Mon Mar 7 05:46:32 2005 Subject: confocal microscopy References: <2j2i5.1183$WM6.88326771@news1.mtl.metronet.ca> Message-ID: Hi Tina, We use the Leica Confocal microscope at ASU. The imaging center must train you on the equipment before you can use the microscope. There are additional training seminars for a more in depth understanding. I have included the link to the Keck Imaging Center at ASU as well as the Leica link. Maybe someone from the Keck lab can direct you further if this does not help. Page http://lsvl.la.asu.edu/Klab/index.html http://www.leica-microsystems.com/ "Tina" wrote in message news:2j2i5.1183$WM6.88326771@news1.mtl.metronet.ca... > Is there any course and/or formation on confocal microscopy, like intensive > theorical and practical course where I could go? > > Any info? > Thanks a lot > Tina > > From webmaster at bioinformatik.de Fri Aug 11 02:43:44 2000 From: webmaster at bioinformatik.de (Andrea Hansen) Date: Mon Mar 7 05:46:32 2005 Subject: Protease cleavage References: <8mu367$fs1$1@ames.central.susx.ac.uk> Message-ID: "Matt Hicks" writes: > Does anyone know of a program for predicting protease cleavage sites (and > size of fragments?)? > Failing that is there a database of proteases with their recognition > sequences so that I can write my own programme to do this? Hi Matt, just try these online tools: Cutter http://delphi.phys.univ-tours.fr/Prolysis/cutter.html or PeptideMass http://www.expasy.ch/tools/peptide-mass.html Andrea -- Institut fuer Botanik III Tel +49 211 81 12339 Heinrich-Heine-Universitaet Fax +49 211 81 13554 Universitaetsstr.1 email : webmaster@bioinformatik.de 40225 Duesseldorf http://www.bioinformatik.de From pd1 at mole.bio.cam.ac.uk Fri Aug 11 06:46:43 2000 From: pd1 at mole.bio.cam.ac.uk (Paul) Date: Mon Mar 7 05:46:32 2005 Subject: Depolymerisation of MT and Actin Cytoskeleton References: <200008081057.LAA02209@ns4.local.nimr> Message-ID: <3993E798.FA1855D7@mole.bio.cam.ac.uk> Sven Pfeiffer wrote: > Hi there, > > I am looking for membrane permeable reagents that depolymerise MT and/or > actin cytoskeleton. Any suggestions are greatly appreciated. > For actin, the various cytochalasins (CD is probably the best characterised/most used) or latrunculin. Paul Digard From kai-uwe.zanzinger at student.uni-tuebingen.de Thu Aug 3 08:25:24 2000 From: kai-uwe.zanzinger at student.uni-tuebingen.de (zxmqi28) Date: Mon Mar 7 05:46:32 2005 Subject: ribozyme Message-ID: <8n12pg$vvk$1@newsserv.zdv.uni-tuebingen.de> dear all, i?m still looking for some informations about ribozymes, because i will start a practical training next week and need some background-informations. thanks a lot kai -------------- next part -------------- An HTML attachment was scrubbed... URL: http://iubio.bio.indiana.edu/bionet/mm/cellbiol/attachments/20000803/772bad0b/attachment.html From rcjohnsen at aol.com Fri Aug 11 23:17:13 2000 From: rcjohnsen at aol.com (Rcjohnsen) Date: Mon Mar 7 05:46:32 2005 Subject: GATEKEEPER" PROTEIN IS KEY TO CELLULAR LIFE; LINKS BACTERIA, CHLOROPLASTS AND MITOCHONDRIA Message-ID: <20000812001713.00800.00000598@ng-bk1.aol.com> (Embargoed for release until 2 p.m. ET Wednesday, August 9, 2000 to coincide with publication in the journal Nature.) "GATEKEEPER" PROTEIN IS KEY TO CELLULAR LIFE; LINKS BACTERIA, CHLOROPLASTS AND MITOCHONDRIA COLUMBUS, Ohio -Researchers here have determined that a seemingly ordinary protein called YidC found within the membranes of bacteria serves as a gatekeeper of sorts, allowing into the membrane other proteins essential for the bacteria to live. When YidC isn't present, the bacteria die. The finding surprised scientists who long believed that certain "independent" proteins were able to pass into the membrane on their own. The new discovery, reported in this week's issue of the journal Nature, may suggest a completely new pathway for the translocation of proteins within basic biological units. Even more startling was the discovery that several other proteins that are remarkably similar to YidC may play similar roles inside mitochondria and in chloroplasts as well. The discovery suggests that bacteria, chloroplasts and mitochondria may all have evolved from a common ancestor. While YidC has been known to be present in cells for some time, researchers were unclear as to what role it might perform, explained Ross Dalbey, professor of chemistry at Ohio State University. Dalbey and Ph.D student James Samuelson were investigating the protein's role in Escherichia coli bacteria as part of a National Science Foundation-supported study. "Proteins are synthesized within the cytoplasm of the cell but they then have to be transported, or inserted, either across or into the membranes of organelles within the cell to do their work," Dalbey said. These membranes function as barriers, he said, blocking proteins and other compounds from areas where they don't belong. In their work, Dalbey and Samuelson developed a strain of the E. coli in which YidC can be depleted. They found that when the YidC was absent, proteins could not migrate into the membranes and the bacteria died. They also looked at another specific protein called Procoat that was considered "independent," that is, researchers believed it would insert itself into the membrane on its own. But in bacteria lacking YidC, even the Procoat was blocked from entering the membrane. "To everyone's surprise, the protein nearly everybody thought inserted spontaneously requires YidC to succeed in entering the membrane," he said. The finding of YidC's role in bacteria is even more important when linked to other research on two other basic organelles - chloroplasts in plants and mitochondria in eukaryotic cells. Other teams have found that in mitochondria, a protein called Oxa1 is known to be required for other proteins to migrate into the mitochondrial inner membrane. Without it, there is no migration and the mitochrondia cannot carry out vital cellular processes. A similar protein called Albino3 in the membranes of chloroplasts inside plant cells functions in apparently the same manner. Without it, the chloroplasts can't function. "The process of membrane insertion is needed for cellular respiration in bacteria and mitochondria, as well as for photosynthesis in chloroplasts," Dalbey said. When scientsts look at the genetic sequences of YidC, Oxa1 and Albino3, Dalbey said, "they are so remarkably similar that it makes you believe they're evolutionarily linked." The researchers hope that uncovering the function of YidC and related proteins may offer new ways of either enhancing cell function or in accelerating cell death - two mechanisms essential in fighting most diseases, Dalbey said. Along with Samuelson and Dalbey, Minyong Chen and Fenglei Jiang, both Ph.D students at Ohio State, Ines Moller and Martin Wiedman of Memorial Sloan-Kettering Cancer Center, Andreas Kuhn of the University of Hohenheim and Greg Phillips of Iowa State University all worked on the project. # Contact: Ross Dalbey, (614) 292-2384; Dalbey@chemistry.ohio-state.edu Written by Earle Holland, (6140 292-8384; Holland.8@osu.edu From rcjohnsen at aol.com Fri Aug 11 23:48:07 2000 From: rcjohnsen at aol.com (Rcjohnsen) Date: Mon Mar 7 05:46:32 2005 Subject: Telomere dysfunction promotes non-reciprocal translocations and epithelial cancers in mice Message-ID: <20000812004807.00800.00000605@ng-bk1.aol.com> Nature 406, 641 - 645 (2000) ? Macmillan Publishers Ltd. Telomere dysfunction promotes non-reciprocal translocations and epithelial cancers in mice STEVEN?E.?ARTANDI*, SANDY?CHANG*?, SHWU-LUAN?LEE*, SCOTT?ALSON*, GEOFFREY?J.?GOTTLIEB??, LYNDA?CHIN* & RONALD?A.?DEPINHO*? *?Department of Adult Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115 , USA ??Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts 02115 , USA ??CPI Ameripath Laboratory, Beachwood, Ohio 44122, USA ??Ackerman Academy of Dermatopathology , New York, New York 10016, USA ?Department of Dermatology, Harvard Medical School, Boston, Massachusetts 02115 , USA ??Departments of Genetics and Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA Correspondence and requests for materials should be addressed to R.A.D. (e-mail: ron_depinho@dfci.harvard.edu). Aged humans sustain a high rate of epithelial cancers such as carcinomas of the breast and colon, whereas mice carrying common tumour suppressor gene mutations typically develop soft tissue sarcomas and lymphomas. Among the many factors that may contribute to this species variance are differences in telomere length and regulation. Telomeres comprise the nucleoprotein complexes that cap the ends of eukaryotic chromosomes and are maintained by the reverse transcriptase, telomerase1. In human cells, insufficient levels of telomerase lead to telomere attrition with cell division in culture2 and possibly with ageing and tumorigenesis in vivo3-5. In contrast, critical reduction in telomere length is not observed in the mouse owing to promiscuous telomerase expression and long telomeres6-10. Here we provide evidence that telomere attrition in ageing telomerase-deficient p53 mutant mice promotes the development of epithelial cancers by a process of fusion-bridge breakage that leads to the formation of complex non-reciprocal translocations?a classical cytogenetic feature of human carcinomas. Our data suggest a model in which telomere dysfunction brought about by continual epithelial renewal during life generates the massive ploidy changes associated with the development of epithelial cancers. From rcjohnsen at aol.com Fri Aug 11 23:58:28 2000 From: rcjohnsen at aol.com (Rcjohnsen) Date: Mon Mar 7 05:46:32 2005 Subject: YidC mediates membrane protein insertion in bacteria Message-ID: <20000812005828.00854.00000596@ng-bk1.aol.com> Nature 406, 637 - 641 (2000) ? Macmillan Publishers Ltd. YidC mediates membrane protein insertion in bacteria JAMES?C.?SAMUELSON*, MINYONG?CHEN*, FENGLEI?JIANG*, INES?M?LLER?, MARTIN?WIEDMANN?, ANDREAS?KUHN?, GREGORY?J.?PHILLIPS? & ROSS?E.?DALBEY* *?Department of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus , Ohio 43210, USA ??Cellular Biochemistry and Biophysic Program, Memorial Sloan-Kettering Cancer Centre, 1275 York Avenue , New York, New York 10021, USA ??University of Hohenheim, Institute for Microbiology and Molecular Biology, Garbenstrasse 30, D-70599 Stuttgart, Germany ??Department of Microbiology, Iowa State University, 207 Science I, Osborn Drive, Ames, Iowa 50011, USA Correspondence and requests for materials should be addressed to R.E.D. (e-mail: dalbey@chemistry.ohio-state.edu). The basic machinery for the translocation of proteins into or across membranes is remarkably conserved from Escherichia coli to humans. In eukaryotes, proteins are inserted into the endoplasmic reticulum using the signal recognition particle (SRP) and the SRP receptor, as well as the integral membrane Sec61 trimeric complex (composed of alpha, beta and gamma subunits)1. In bacteria, most proteins are inserted by a related pathway that includes the SRP homologue Ffh2-5, the SRP receptor FtsY6, 7, and the SecYEG trimeric complex8, where Y and E are related to the Sec61 alpha and gamma subunits, respectively. Proteins in bacteria that exhibit no dependence on the Sec translocase were previously thought to insert into the membrane directly without the aid of a protein machinery9, 10. Here we show that membrane insertion of two Sec-independent proteins requires YidC. YidC is essential for E. coli viability and homologues are present in mitochondria and chloroplasts. Depletion of YidC also interferes with insertion of Sec-dependent membrane proteins, but it has only a minor effect on the export of secretory proteins. These results provide evidence for an additional component of the translocation machinery that is specialized for the integration of membrane proteins. From robnic1 at email.msn.com Sat Aug 12 11:05:28 2000 From: robnic1 at email.msn.com (ROBERT WILLIAMS) Date: Mon Mar 7 05:46:32 2005 Subject: e coli Message-ID: hi! recently i have been noticing that when culturing my e. coli cells there is a distinct order (not the normal smell of the cells). Does anyone know if it could posibly be contamination or are the cells overgrowing? thanks in advance!!! From hhand.91 at hotmail.com Sat Aug 12 17:30:44 2000 From: hhand.91 at hotmail.com (PianoMan) Date: Mon Mar 7 05:46:32 2005 Subject: Play your Piano using this patented instructional device. Message-ID: <20000812223035.BA724415D5@mercury.hgmp.mrc.ac.uk> Piano owners. We guarantee you will play a recognizable melody upon recieving this incredible technology for your piano. Click on the Email address below..and enter "More info" on the subject line and click the Send icon. We will send you complete information about this incredible device that will make you an instant Piano Player. It's truely amazing! Pianoman@iar.net If you have recieved this in error please click on the email address below, type "remove" on the subject line and send. hhand81@hotmail.com This ad is being sent in compliance with Senate bill 1618, Title 3, section 301, Paragraph (a)(2)(c) --- From rcjohnsen at aol.com Sat Aug 12 18:29:49 2000 From: rcjohnsen at aol.com (Rcjohnsen) Date: Mon Mar 7 05:46:32 2005 Subject: NO is necessary and sufficient for egg activation at fertilization Message-ID: <20000812192949.27826.00000389@ng-bh1.aol.com> Nature 406, 633 - 636 (2000) ? Macmillan Publishers Ltd. NO is necessary and sufficient for egg activation at fertilization RICHARD?C.?KUO*?, GREGORY?T.?BAXTER?, STUART?H.?THOMPSON*?, STEPHEN?A.?STRICKER?, CHRIS?PATTON?, JOSEPH?BONAVENTURA? & DAVID?EPEL? *?Neurosciences Program, Stanford University School of Medicine, Stanford University, Stanford, California 94305, USA ??Department of Biological Sciences, Hopkins Marine Station, Stanford University, Pacific Grove, California 93950, USA ??Cornell Nanofabrication Facility, Knight Laboratory, Cornell University, Ithaca, New York 14853, USA ??Department of Biology, University of New Mexico, Albuquerque, New Mexico 87131 , USA ?Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA ??Nicholas School of the Environment, Duke Marine Biomedical Center, Pivers Island, North Carolina 28516, USA Correspondence and requests for materials should be addressed to D.E. (email: depel@stanford.edu). The early steps that lead to the rise in calcium and egg activation at fertilization are unknown but of great interest?particularly with the advent of in vitro fertilization techniques for treating male infertility and whole-animal cloning by nuclear transfer. This calcium rise is required for egg activation and the subsequent events of development in eggs of all species1, 2. Injection of intact sperm or sperm extracts can activate eggs, suggesting that sperm-derived factors may be involved. Here we show that nitric oxide synthase is present at high concentration and active in sperm after activation by the acrosome reaction. An increase in nitrosation within eggs is evident seconds after insemination and precedes the calcium pulse of fertilization. Microinjection of nitric oxide donors or recombinant nitric oxide synthase recapitulates events of egg activation, whereas prior injection of oxyhaemoglobin, a physiological nitric oxide scavenger, prevents egg activation after fertilization. We conclude that nitric oxide synthase and nitric-oxide-related bioactivity satisfy the primary criteria of an egg activator: they are present in an appropriate place, active at an appropriate time, and are necessary and sufficient for successful fertilization. From goboto0258 at amjwuptsa.hotepmail.com Sun Aug 13 12:41:43 2000 From: goboto0258 at amjwuptsa.hotepmail.com (goboto0258@amjwuptsa.hotepmail.com) Date: Mon Mar 7 05:46:33 2005 Subject: You have done the work..NOW GAT PAID! -wsksya Message-ID: <0nmiys40h40r4ybr.gykx1g3@2gh2kg3.co.za> An HTML attachment was scrubbed... URL: http://iubio.bio.indiana.edu/bionet/mm/cellbiol/attachments/20000813/cca3fd81/attachment.html From clarosa at biocomp.unl.edu Sun Aug 13 12:49:16 2000 From: clarosa at biocomp.unl.edu (Chris LaRosa) Date: Mon Mar 7 05:46:33 2005 Subject: e coli References: Message-ID: <3996DF9C.5EE3F887@biocomp.unl.edu> ROBERT WILLIAMS wrote: > > hi! recently i have been noticing that when culturing my e. coli cells there > is a distinct order (not the normal smell of the cells). Does anyone know if > it could posibly be contamination or are the cells overgrowing? thanks in > advance!!! usually odd odor indicates contamination. Try streaking out the cells and examining the differences in colony morphology. You should always have a frozen stock to make a new starter, if you are in doubt, start from a new single colony in case the cells have mutated. From ilan at netfever.com Sun Aug 13 16:01:01 2000 From: ilan at netfever.com (Ilan) Date: Mon Mar 7 05:46:33 2005 Subject: cathepsin D Message-ID: <39970C8D.8336DDD1@netfever.com> Is there anybody who know how to purify cathepsin D ? Thanks From Ulriks at ruc.dk Mon Aug 14 04:03:25 2000 From: Ulriks at ruc.dk (Ulrik) Date: Mon Mar 7 05:46:33 2005 Subject: TX-100 soulubility? Message-ID: <8n8cjs$8bi$1@news.online.de> Hello everybody Im a second year student of molecular biology, and have just startet on a project about connexins and their assembly into connexons. In my readings I find that Triton X-100 soulubility is used to tell something about the proteins, but what? Can anyone in this newsgroup please tell me what the TX-100 soulubility is good for. Has it got something to do with phosphorylation of the proteins? Thanks Ulrik From BRONE at Lifetech.com Mon Aug 14 20:11:00 2000 From: BRONE at Lifetech.com (Rone-Clarke, Barbara) Date: Mon Mar 7 05:46:33 2005 Subject: Suitable fibroblast for Horse Serum Message-ID: <9FE70897842FD411A0B100E018C23E48184069@NZSERV1> I am trying to find a suitable fibroblast cell line, preferably lung derived, which will grow well in media supplemented with horse serum. I have tried MRC-5 and WI38 cells in RPMI with mixed success so far. Any help would be appreciated Thanks Ray Gilbert Senior Cell Culture Technician LTL Australasia --- From mdudek at home.com Mon Aug 14 20:48:56 2000 From: mdudek at home.com (Miro) Date: Mon Mar 7 05:46:33 2005 Subject: Help Message-ID: Does anyone know any web addresses to learn about protein purification, protein extraction, use of Hplc and protein purification Sylvia From mucineer at iname.com Tue Aug 15 06:36:42 2000 From: mucineer at iname.com (Student) Date: Mon Mar 7 05:46:33 2005 Subject: Methotrexate amplification Message-ID: <8nba05$cos$1@oyez.ccc.nottingham.ac.uk> Dear All I have a question which is puzzling me. After transfection and selection (with media lacking ribo/deoxyribonucleosides and G418) of a CHO cell line trasnfected with my plasmid of interest, it is possible to "amplify" the copy number of the gene in the host chromosome by adding increasing amounts of methotrexate. Exactly how do these pieces of DNA which is already incorporated into the host chromosome "increase in number"? Any insights will be appreciated. Thanks. From mailerror at yeah.net Tue Aug 15 13:19:46 2000 From: mailerror at yeah.net (debugemail) Date: Mon Mar 7 05:46:33 2005 Subject: debugemail Message-ID: <20000815181943.0E8D8415D5@mercury.hgmp.mrc.ac.uk> ?????????????????????????????????? ?????????????????????????????????????????????????? ?????????????????? debugemail.yeah.net ??????????????EMAIL????????350???????????? ??????????????????SMTP????????300?? ??????????????????????EMAIL?????????????????????????? ?????????????????????????????? -------------- next part -------------- A non-text attachment was scrubbed... Name: email.zip Type: application/octet-stream Size: 416 bytes Desc: email.zip Url : http://iubio.bio.indiana.edu/bionet/mm/cellbiol/attachments/20000815/b6ae6a7a/email.exe From martin.offterdinger at akh-wien.ac.at Wed Aug 16 07:50:06 2000 From: martin.offterdinger at akh-wien.ac.at (Martin Offterdinger) Date: Mon Mar 7 05:46:33 2005 Subject: Methotrexate amplification References: <8nba05$cos$1@oyez.ccc.nottingham.ac.uk> Message-ID: <8ne1l7$5sk0$1@www.univie.ac.at> Student schrieb in im Newsbeitrag: 8nba05$cos$1@oyez.ccc.nottingham.ac.uk... > Dear All > > I have a question which is puzzling me. After transfection and selection > (with media lacking ribo/deoxyribonucleosides and G418) of a CHO cell line > trasnfected with my plasmid of interest, it is possible to "amplify" the > copy number of the gene in the host chromosome by adding increasing amounts > of methotrexate. > > Exactly how do these pieces of DNA which is already incorporated into the > host chromosome "increase in number"? > > Any insights will be appreciated. Thanks. > This is a well documented system; These are the basics 1) Your parental CHO cell line is dihydrofolatereductase-deficient (DHFR-) and can therefore not make nucleosides 2) On your plasmid with you gene of interest you have the DHFR gene present 3)MTX is an DHFR-inhibitor (competitive!) 4) By using MTX the medium you force the cells to produce even more DHFR (and to coampflify your gene of interest) 5) Mechanism: This is due to gene amplification. (Selective DNA reduplication on the region of the chromosome where your plasmid has been integrated upon selection) You should also check out the literature on this; it is used for several years already!!! Martin From jyuh at mail.nsysu.edu.tw Wed Aug 16 20:41:59 2000 From: jyuh at mail.nsysu.edu.tw (Guh) Date: Mon Mar 7 05:46:33 2005 Subject: Type I Collagens are usually secreted as procollagen triple helices? Message-ID: <399B42E7.3F0BA2D8@mail.nsysu.edu.tw> Dear all: Type I Collagens are usually secreted as procollagen triple heliices (2 alpha1 chains and 1 alpha 2 chain). But I wonder whether or not it can be secreted as the isolated alpha 1 and alpha 2 chains? Does anyone has references? Thanks a lot. -- Sincerely Yours, Jinn-Yuh Guh, M.D. Dept. of Internal Medicine Kaohsiung Medical College 100 Shi-Chuan 1st Road Kaohsiung, Taiwan FAX: 886-7-312-2810 e-mail: jiyugu@cc.kmu.edu.tw From mailerror at yeah.net Thu Aug 17 07:44:51 2000 From: mailerror at yeah.net (MCSE2BE) Date: Mon Mar 7 05:46:33 2005 Subject: MCSE2BE Message-ID: <200008171215.IAA18776@capricorn.netsurf.net> ??????????????????????????????????????????????? ???????????????? mcse2be.myetang.com ******????????****** ???????????????????????????????????? ????IT???????????????????????????? -------------- next part -------------- A non-text attachment was scrubbed... Name: mcse.zip Type: application/octet-stream Size: 312 bytes Desc: mcse.zip Url : http://iubio.bio.indiana.edu/bionet/mm/cellbiol/attachments/20000817/67a52e88/mcse.exe From Leon at caresystems.com.au Thu Aug 17 18:57:21 2000 From: Leon at caresystems.com.au (Leon) Date: Mon Mar 7 05:46:33 2005 Subject: simple question Message-ID: hi i am rather a new to this field was just wondering why is it that some cells to no regenerate (eg if ones arm gets damaged it does not regrow) and yet some cells keep regrowing (eg hair , nails)? From Leon at caresystems.com.au Thu Aug 17 18:57:56 2000 From: Leon at caresystems.com.au (Leon) Date: Mon Mar 7 05:46:33 2005 Subject: cell id Message-ID: in terms of cells that do not continually regenerate - do they each have their own unique id tag? From alternativeframe at altavista.com.SXBW Fri Aug 18 10:20:24 2000 From: alternativeframe at altavista.com.SXBW (alternativeframe@altavista.com.SXBW) Date: Mon Mar 7 05:46:33 2005 Subject: Colorplak Framing Message-ID: <20000818151928.D7435415D4@mercury.hgmp.mrc.ac.uk> Colorplak? Framing AFFORDIBLE CUSTOM FRAMING ALTERNATIVE... Colorplak is so unique that even Kodak uses it! ALTERNATIVE FRAMING BY ICON is now extending this exclusive limited time offer to E-mail subscribers. You can enjoy all the benefits of beautiful Colorplak custom framing at a tremendously affordable sale price. IF IT'S MADE OF PAPER OR CANVAS ALTERNATIVE FRAMING CAN FRAME IT FOR YOU!!! Colorplak's are great for posters, certificates, desktop d?cor, family photos, wedding pictures, point-of-sale displays, exhibits, maps, gifts and more. Colorplak's? Unique Features: ? Durable Fiberboard Mount ? Easy to clean ? UV Coating Resists Fading ? Exhibit on wall or desk ? Professional Non-Glare Finish (No breakable glass) NOW OFFERING 34 EDGE COLORS! The Colorplak custom framing alternative is offered in three styles, 34 edge colors, and up to 4 x 8 feet, which means there's a perfect Colorplak frame to complement any image or d?cor. Compare the cost of Colorplak with Custom Picture Framing. Colorplak is an Excellent Value. Call for E-mail address info. Frame those prize possessions with Colorplak's?. It's SIMPLE! It's EASY! It's AFFORDABLE! CALL 1 (888) 918-4767 24 HOURS A DAY!!! We accept Master Card, Visa, and American Express This is a one time mailing!!! Please pardon the intrusion.... Under Bill s.1618 TITLE III passed by the 105th US Congress this letter cannot be considered Spam as long as the sender includes "contact information" & a method of "removal". To be removed, simply type: "Remove" in the subject box. --- From rcjohnsen at aol.com Sat Aug 19 14:24:50 2000 From: rcjohnsen at aol.com (Rcjohnsen) Date: Mon Mar 7 05:46:33 2005 Subject: Stem cells regenerate skin and hair Message-ID: <20000819152450.14659.00000150@ng-fo1.aol.com> Stem cells regenerate skin and hair EMBARGOED FOR RELEASE: 17 AUGUST 2000 AT 05:00 ET US Contact: Ellen O'Brien obriene@uphs.upenn.edu 215-349-5659 University of Pennsylvania Medical Center Skin and hair spring from the same stem cells, researchers find Finding advances understanding of skin wounds and skin cancer In a study that has implications for understanding the way skin wounds heal and some skin cancers develop, researchers at the University of Pennsylvania Medical Center and New York University School of Medicine have found compelling evidence that the hair follicle and the epidermis may originate from the same cache of cells. The researchers have traced the earliest daughters of the stem cells (primitive cells not yet committed to a specific developmental pattern) to the section of the upper follicle adjacent to the region where the stem cells reside. The finding shows clearly for the first time how cells from the hair follicle can directly influence the epidermis. "Our results suggest that in normal newborns, and in healing wounds throughout life, it is the daughter cells in the upper follicle that migrate upward to form and maintain the new epidermis. The daughter cells of the stem cells also migrate downward to form the hairshaft," said Robert M. Lavker, PhD., professor of dermatology at the University of Pennsylvania School of Medicine. Lavker has been investigating stem cell systems for nearly 20 years in collaboration with Tung-Tien Sun, PhD, of the NYU School of Medicine. Their current work identifies the upper follicle as the site of young transient amplyfying (TA) cells -- which are the early offspring of stem cells -- and follows the TA cells?migration into the epidermis and into the bulb, or root, of the hair where the TA cells differentiate into components of the hair shaft. The current study, which will be published in the August 18 issue of Cell, builds on the 1990 research of Lavker and his coworkers, in which they located stem cells at the bulge of the follicle (the point where the outer root sheath attaches to the arrector pili muscle). The new study furnishes evidence that these researchers were correct at the time when they also postulated that the bulge stem cells are bipotent ? capable of generating TA cells that develop along two distinct paths. Lavker and Sun had proposed that the stem cells were bipotent in order to explain a phenomenon that scientists had been aware of for years, but had never been able to understand: Healthy skin seems capable of self-regeneration. But when a severe burn destroys the skin, epidermal cells are found to emerge from any remaining hair follicles to re-establish the outer skin in circles of re-growth. The precise origin of those new epidermal cells had never been established. Lavker and Sun?s theory that bulge stem cells were the source of both epidermal cells and hair cells became the basis of their current research. Using young and adult mice, Lavker, Sun and their colleagues devised a double-labeling technique in which they could follow the division of the stem cells, and then observe the trafficking patterns of the early-offspring TA cells. Results from the study "strongly suggest that the bulge stem cell is bipotent, and that the daughter (TA) cells migrate up to the epidermis and down to the root," Lavker said. Added Sun: "There?s been a termendous controversy as to where the epidermal stem cells are. We are proposing that there is just one entity -- an ultimate epidermal stem cell -- located in the bulge area of the hair follicle, that is capable of forming skin or hair." A major "take home message" of the research, Lavker said, is the pivotal importance of the upper follicle in the healing process of the skin: "It places the upper follicle directly in the center, for wound repair." The work also paves the way for designing effective skin cancer treatment, by explaining why skin tumors that are produced for the purpose of research frequently originate in the upper follicle. "In addition to wanting to target stem cells in the bulge for skin cancer (stem cells are the prime source of abnormal growth and mutation), now you also have to consider targeting the upper follicle where the TA cells retain most characteristics of the stem cells," Lavker said. ### Participating with Lavker and Sun in the research were Michael Lehrer, MD., and Pamela Jensen, PhD., of Penn, and Gina Taylor, MD., of Presbyterian Hospital. The work was funded by the National Institutes of Health. From smallbiz at newz00.dynip.com Sat Aug 19 18:50:07 2000 From: smallbiz at newz00.dynip.com (Small Business Newsletter) Date: Mon Mar 7 05:46:34 2005 Subject: Congratulations...You Got Web Site! Message-ID: Got Web Site? For a limited time, we are offering a custom designed Web Site up to 3 pages for only $445! We will also include: - 1 year hosting fees (a $180 value) - Domain Name Registration Fee (1 year=$35) If you Sign up until: August 25/00 We will offer FREE Promotion: - Search Engines submission: All major search engines+Index sites - over 3000 ( a $145 value) - Your classified Ad posted to 500+ Classifieds web sites ($50 value)! - Your site promoted to 100.000 e-mail recipients (a $500 value) sign Up now at: http://www.onesmalldot.com/le/index.html or Call Toll Free: 1-877-599-3141 See You Online! www.onesmalldot.com ==================================================================== NOTICE: To unsubscribe from the Small Business Newsletter: Please, click the email link below and send. --- From smallbiz at newz00.dynip.com Sat Aug 19 19:38:05 2000 From: smallbiz at newz00.dynip.com (Small Business Newsletter) Date: Mon Mar 7 05:46:34 2005 Subject: Congratulations...You Got Web Site! Message-ID: Got Web Site? For a limited time, we are offering a custom designed Web Site up to 3 pages for only $445! We will also include: - 1 year hosting fees (a $180 value) - Domain Name Registration Fee (1 year=$35) If you Sign up until: August 25/00 We will offer FREE Promotion: - Search Engines submission: All major search engines+Index sites - over 3000 ( a $145 value) - Your classified Ad posted to 500+ Classifieds web sites ($50 value)! - Your site promoted to 100.000 e-mail recipients (a $500 value) sign Up now at: http://www.onesmalldot.com/le/index.html or Call Toll Free: 1-877-599-3141 See You Online! www.onesmalldot.com ==================================================================== NOTICE: To unsubscribe from the Small Business Newsletter: Please, click the email link below and send. --- From amichal at uia.ua.ac.be Sun Aug 20 18:31:08 2000 From: amichal at uia.ua.ac.be (Andrej.Michalik) Date: Mon Mar 7 05:46:34 2005 Subject: pTK-Neo or similar vector Message-ID: Dear netters, I am looking for a vector containing Neo selection marker under the control of TK promoter, which can be used for cotransfection with a marker-less construct and subsequent selection of stables. Anyone has this or has heard of anyone having it? Thanx a lot andrej ------------------------------------------------------------------------------ A andrej michalik A A department of biochemistry A A university of antwerp (uia) A A universiteitsplein 1 AAAAAAAAAAAAA B-2610 antwerpen A AAA belgium A A tel: +32-(0)3-8202642 amichal@uia.ac.be ------------------------------------------------------------------------------ From pd1 at mole.bio.cam.ac.uk Mon Aug 21 05:31:31 2000 From: pd1 at mole.bio.cam.ac.uk (Paul) Date: Mon Mar 7 05:46:34 2005 Subject: Suitable fibroblast for Horse Serum References: <9FE70897842FD411A0B100E018C23E48184069@NZSERV1> Message-ID: <39A10503.FA893009@mole.bio.cam.ac.uk> "Rone-Clarke, Barbara" wrote: > I am trying to find a suitable fibroblast cell line, preferably lung > derived, which will grow well in media supplemented with horse serum. > > I have tried MRC-5 and WI38 cells in RPMI with mixed success so far. > > Any help would be appreciated > > A colleague successfully grew COS cells in horse serum (to generate Q deficient tRNAs). He said they looked a bit granular after a while but the division time didn't change noticeably. Paul Digard From swr at crc.dk Mon Aug 21 09:19:57 2000 From: swr at crc.dk (Soeren W. Rasmussen) Date: Mon Mar 7 05:46:34 2005 Subject: DNATools revision 716 Message-ID: <39A13A8D.82C09519@crc.dk> Dear DNATools user, Please note that the new revision of the program (from revision 709 onwards) requires reinstalling the program. You can read more about this revision and download the new setup files at: http://www.dnatools.dk Regards Soeren -- Dr. scient. Soeren W. Rasmussen Carlsberg Laboratory, Department of Yeast Genetics 10 Gl. Carlsbergvej, DK-2500, Copenhagen, Denmark phone: 45 3327 5230, mail: swr@crc.dk http://www.crc.dk/phys/ DNATools sequencing software: http://www.dnatools.dk From hoevel at geocities.com Mon Aug 21 09:48:33 2000 From: hoevel at geocities.com (SciMedWeb) Date: Mon Mar 7 05:46:34 2005 Subject: molecular breast cancer Message-ID: <8nrnmi$nmb$4@news.planetinternet.be> Molecular breast cancer: http://www.geocities.com/m.lacroix/intro1.htm From haejin at netinfo.ubc.caX Mon Aug 21 15:44:31 2000 From: haejin at netinfo.ubc.caX (Austin P. So (Hae-Jin)) Date: Mon Mar 7 05:46:34 2005 Subject: TX-100 soulubility? References: <8n8cjs$8bi$1@news.online.de> Message-ID: <39A194AF.A902AF56@netinfo.ubc.caX> membrane-associated vs. cytosolic... Ulrik wrote: > Im a second year student of molecular biology, and have just startet on a > project about connexins and their assembly into connexons. In my readings I > find that Triton X-100 soulubility is used to tell something about the > proteins, but what? -- --- Austin P. So (Hae Jin) I.I.S.G.P. Biotechnology Laboratory University of British Columbia E-mail: haejin@netinfo.ubc.ca http://www.interchange.ubc.ca/haejin/index.html (under construction) From schultz at uleth.ca Mon Aug 21 17:47:11 2000 From: schultz at uleth.ca (Elizabeth Schultz) Date: Mon Mar 7 05:46:34 2005 Subject: tenure-track positions Message-ID: <39A1B372.814B5704@uleth.ca> The Department of Biological Sciences at the University of Lethbridge, Alberta, Canada, seeks applications for Two Assistant Professors, probationary (tenure-track) beginning July 2001, subject to budgetary approval. Preference will be given to individuals with demonstrated research strengths in the fields of: 1) plant molecular biology, plant fungal interactions, plant pathology, or agricultural biotechnology for one position; and 2) cell and molecular biology and/or genetics for the other. Individuals doing research relating to cell structure and function, cell physiology, virology, immunology, molecular genetics, or developmental biology are encouraged to apply. The successful applicants will be required to teach upper and lower level courses in areas such as molecular and cellular biology, genetics and biotechnology, including introductory courses in Cellular Basis of Life and/or Diversity of Life. Opportunities exist for the supervision of Graduate students and for research collaborations with scientists on campus as well as at Federally-supported research facilities in agriculture, human and animal health. Biological research at the University is supported by a number of excellent facilities including electron and confocal microscopy, a new phytotron, an ABI automated DNA sequencer, NMR and mass spectroscopy. The University aspires to hire individuals who have demonstrated considerable potential for excellence in teaching, research and scholarship, and especially those who have well-established research programs. The University is an equal opportunity employer and offers a non-smoking environment. New Faculty are eligible to apply for university funding in support of research and scholarly activities. Lethbridge is an attractive city of 70,000 situated in Southern Alberta, close to National Parks and Wilderness areas of the Rocky Mountains and Cypress Hills. Founded in 1967, the University focuses on excellence in undergraduate programs and has an enrollment of over 6,000 students. The University is in an expansion phase and, among other projects, is building a $37-million Library Information Network Centre (LINC). For more information about the University please visit our web site at www.uleth.ca. A Ph.D. is required. Post-doctoral and teaching experience are assets. In accordance with Canadian Immigration Regulations, this advertisement is directed to Canadian citizens and permanent residents of Canada. Applications should include a curriculum vitae, transcripts, outlines of courses previously taught, teaching evaluations and publication reprints or preprints, a statement of teaching philosophy, a statement of proposed research, and names of at least three referees who are scholars in the field. Send this material and arrange for the letters of reference to be mailed directly to: Dr. John Bain, Chair, Department of Biological Sciences, The University of Lethbridge, 4401 University Drive, Lethbridge, Alberta, T1K 3M4. Telephone: (403) 329-2245, Fax: (403) 329-2082, or E-mail: bain@uleth.ca. The closing date for applications is October 16, 2000. --- From gildasom at surfline.ne.jp Tue Aug 22 06:50:09 2000 From: gildasom at surfline.ne.jp (gildasom@surfline.ne.jp) Date: Mon Mar 7 05:46:34 2005 Subject: Complete E-commerce Solutions !!! Message-ID: <6251068374.sjt83517@rly-v74513.surfline.ne.jp> Complete E-COMMERCE solutions to accept VISA, MasterCard, American Express, Discover and Checks for your website, online store, traditional storefront or home based business. WE OFFER -Bank-approved merchant accounts -Real-time, online payment transactions -Shopping carts -Terminals & printers -95% approval rate -Fast 5 - 7 day setup A TURNKEY E-COMMERCE SOLUTION FOR -Internet Storefront Businesses -Mail Order and Phone Order -Start-up Businesses -Traditional Retail Stores -Home based Businesses Good Credit / Bad Credit / No Credit **** NO PROBLEM **** ------FREE SET-UP------FREE SET-UP------FREE SET-UP---------- While OTHERS Charge You From $200 TO $350 To Get Set Up WE CHARGE ZERO FOR SETUP FEES!!! Limited Time Offer So Take Advantage NOW!!! Secure transactions are authorized in true real-time immediately upon submitting orders right on your website. You can process transactions right over the Internet without the need for separate transaction terminal or processing software. Dedicated data line for fast, 3-5 second transactions. No installation required! Quick and easy account setup: 5 - 7 days. _____________________________________________ Full Service E-Commerce Provider who offers complete e-commerce solutions for thousands of businesses. FREE CONSULTATION WITH AN INDUSTRY EXPERT, NO OBLIGATION -LOWEST MONTHLY SERVICE FEES as low as 1.59% Banks can charge up to 5% or higher ------FREE SET-UP------FREE SET-UP------FREE SET-UP-------- The Time Is Right!!! Call Now!!! ------------------------------------------------------------- To have a CONSULTANT contact you, please call us toll free at: 1-888-248-6515 And leave the following info. Name_____________________________________ Phone_______________________ Best Time To Call_____Hawaii - Pacific - Mountain - Central - Eastern Company Name______________________________ Type of Product______________________________ A merchant account CONSULTANT will contact you soon. ============================================== 1-888-248-6515 Here's what our customers say... Testimonial # 1 - I knew having a merchant account would increase my sales, But never thought it would be so great. In addition in being able to take major credit cards, I can also do real time credit card processing on the internet and receive orders while I am Sleeping. It's awesome! I encourage every serious business owner to get one. Thanks. M.B./MI Testimonial # 2 - " Being a home based business owner, no one would approve me, until this came my way. I am more than grateful. Within 10 days I had my merchant account set up. I am more than pleased with the 24 hr. customer service. My business has sky rocketed because I now can accept credit card orders. " Oscar/FL -------------------------------------------------------- To be removed from any future mailings, send email to "lindalou@india.com" and type "Remove" in the subject line. We appologize for any inconvenience. ****************************************************** ************************************** --- From rogier666 at my-deja.com Tue Aug 22 10:04:41 2000 From: rogier666 at my-deja.com (Rogier) Date: Mon Mar 7 05:46:34 2005 Subject: confocal microscopy References: <2j2i5.1183$WM6.88326771@news1.mtl.metronet.ca> Message-ID: <8nu4pq$imn$1@nnrp1.deja.com> Hi Tina, confocal course anywhere on the planet, or not? would help if you tell us what country/city you're in. There's a pretty good confocal course here in Amsterdam, in the lab of the people who invented confocal microscopy. See you around here ? Cya, Rogier Stuger Dept MicFizz, Free U of A E rogier AT biogate DOT com (spammers will be killed) do NOT use the my-deja.com address. I never read that one Sent via Deja.com http://www.deja.com/ Before you buy. From lakenetshop at sinaman.com Wed Aug 23 04:27:07 2000 From: lakenetshop at sinaman.com (LakeNETshop) Date: Mon Mar 7 05:46:34 2005 Subject: PANTONE Special Offer Message-ID: <419.436761.72364525lakenetshop@sinaman.com> ¿Ë·Rªº«È¤á¡G ¥þ·s PANTONE Color Formula Guide (#2007) ¸g¤v±À¥X¡AÃC¦â¼W¦Ü1,114ºØ¡C PANTONE Color Formula Guide (#2007) ¹s°â»ùHK$690.00¡A ²{±À¼s»ùHK$620.00¡A¦A°e APLI Design Paper 12±i¸Ë¨â¥]¡A¦P®É ¦pªG§A¦³¥ô¦óªº Color Guide¡A¥»¤½¥q¥H³Ì°ªHK$100.00¦¬¦^¡C ±À¼s´Á¦Ü2000¦~9¤ë30¤é¡A³f«~¦³­­¡A°â§¹§Y¤î¡C ÁʶR¸Ô±¡½Ð°Ñ¾\¥H¤Uºô­¶ http://www.LakeNETshop.com ½ÐÀH®É¨ì http://www.lake.com.hk ÂsÄý³Ì·sªºÀu´f¤Î®ø®§¡C LakeNETshop Dear Customers, The New Edition PANTONE Color Formula Guide is available now, color increase to 1,114 The Retail price of PANTONE Color Formula Guide (#2007) is HK$690.00 Now Special Offer HK$620.00, and you can get 2 packs (12's) APLI Design Paper FREE, Also if you have any old Color Guide , it worth HK$100.00 Special Offer is until 30-Sep-2000. For Purchase detail please goto http://www.LakeNETshop.com and Please visit our web site at http://www.lake.com.hk for update news. LakeNETshop --- From icshin at bioneer.kaist.ac.kr Wed Aug 23 11:40:08 2000 From: icshin at bioneer.kaist.ac.kr (Incheol Shin) Date: Mon Mar 7 05:46:34 2005 Subject: Apo-Brdu for adherent cell apoptosis ? Message-ID: <8o0up8$9qn$1@green.kreonet.re.kr> Hi, all I am planning to purchase Pheonix Apo-Brdu apoptosis assay system (for flow cytometry) to access apoptosis in my breast cancer cultured cell system. Actually I tested my apoptotic cells with Annexin-V apoptosis assay kit, but the result was not good as expected since (in my opionion) Annexin-V system is basically for the suspension cells. So anyone out there ever did some apoptosis assay with Apo-Brdu assay kit for the adherent cells ? Please give me your opinions. Also, if you know the best apoptosis assay system for the adherent cells. I would like to avoid the system with microscopic observations since I need to include the floating (apoptotic) cells in my assay. Thanks in advance, Incheol -- o o It was a wedding ring, \ __\\___ o Destined to be found in a cheap hotel, \/ o \ o Lost in a kitchen sink, /\_<_____/ Or thrown in a wishing well. / - Warm Wet Circles - Fish From basjhj at nospam.nl Thu Aug 24 02:16:09 2000 From: basjhj at nospam.nl (Bas Jansen) Date: Mon Mar 7 05:46:34 2005 Subject: Apo-Brdu for adherent cell apoptosis ? References: <8o0up8$9qn$1@green.kreonet.re.kr> Message-ID: <8o2i3r$n78$1@odysseus.uci.kun.nl> Incheol Shin heeft geschreven in bericht <8o0up8$9qn$1@green.kreonet.re.kr>... >Also, if you know the best apoptosis assay system >for the adherent cells. I would like to avoid >the system with microscopic observations since >I need to include the floating (apoptotic) cells >in my assay. Can't you just collect the medium with floating cells, and then trypsinize your adherent cells and do your thing? Regards, Bas From hojoseph at neuron.neurosurgeryery.washington.edu Thu Aug 24 20:22:22 2000 From: hojoseph at neuron.neurosurgeryery.washington.edu (Joe) Date: Mon Mar 7 05:46:34 2005 Subject: cell cycle separation Message-ID: <8o4hoe$1q5o$1@nntp4.u.washington.edu> What's the best way to separate cells based on cell cycle phase? I read about something called "counterflow centrifugal elutriation", but have no idea what it is. Can you actually separate cells based on cell cycle phase using FACS or does it just detect. Basically, I want to have different pools of cell enriched for each phase of the cell cycle. Thanks for any info! From martin.offterdinger at akh-wien.ac.at Fri Aug 25 02:56:23 2000 From: martin.offterdinger at akh-wien.ac.at (Martin Offterdinger) Date: Mon Mar 7 05:46:34 2005 Subject: cell cycle separation References: <8o4hoe$1q5o$1@nntp4.u.washington.edu> Message-ID: <8o57pb$3rfi$1@www.univie.ac.at> Joe schrieb in im Newsbeitrag: 8o4hoe$1q5o$1@nntp4.u.washington.edu... FACScan can only detect, if you want to sort cells you have to have a cell sorter. Alternatively cells can be separated according to their denisity in a special centrifuge (G1 cells contain less DNA and are therefore less dense.... than G2/M cells) From rcjohnsen at aol.com Fri Aug 25 16:51:58 2000 From: rcjohnsen at aol.com (Rcjohnsen) Date: Mon Mar 7 05:46:34 2005 Subject: Skin and hair spring from the same stem cells Message-ID: <20000825175158.02432.00001707@ng-fo1.aol.com> EMBARGOED FOR RELEASE: 17 AUGUST 2000 AT 05:00 ET US Contact: Ellen O'Brien obriene@uphs.upenn.edu 215-349-5659 University of Pennsylvania Medical Center Skin and hair spring from the same stem cells, researchers find Finding advances understanding of skin wounds and skin cancer In a study that has implications for understanding the way skin wounds heal and some skin cancers develop, researchers at the University of Pennsylvania Medical Center and New York University School of Medicine have found compelling evidence that the hair follicle and the epidermis may originate from the same cache of cells. The researchers have traced the earliest daughters of the stem cells (primitive cells not yet committed to a specific developmental pattern) to the section of the upper follicle adjacent to the region where the stem cells reside. The finding shows clearly for the first time how cells from the hair follicle can directly influence the epidermis. "Our results suggest that in normal newborns, and in healing wounds throughout life, it is the daughter cells in the upper follicle that migrate upward to form and maintain the new epidermis. The daughter cells of the stem cells also migrate downward to form the hairshaft," said Robert M. Lavker, PhD., professor of dermatology at the University of Pennsylvania School of Medicine. Lavker has been investigating stem cell systems for nearly 20 years in collaboration with Tung-Tien Sun, PhD, of the NYU School of Medicine. Their current work identifies the upper follicle as the site of young transient amplyfying (TA) cells -- which are the early offspring of stem cells -- and follows the TA cells?migration into the epidermis and into the bulb, or root, of the hair where the TA cells differentiate into components of the hair shaft. The current study, which will be published in the August 18 issue of Cell, builds on the 1990 research of Lavker and his coworkers, in which they located stem cells at the bulge of the follicle (the point where the outer root sheath attaches to the arrector pili muscle). The new study furnishes evidence that these researchers were correct at the time when they also postulated that the bulge stem cells are bipotent ? capable of generating TA cells that develop along two distinct paths. Lavker and Sun had proposed that the stem cells were bipotent in order to explain a phenomenon that scientists had been aware of for years, but had never been able to understand: Healthy skin seems capable of self-regeneration. But when a severe burn destroys the skin, epidermal cells are found to emerge from any remaining hair follicles to re-establish the outer skin in circles of re-growth. The precise origin of those new epidermal cells had never been established. Lavker and Sun?s theory that bulge stem cells were the source of both epidermal cells and hair cells became the basis of their current research. Using young and adult mice, Lavker, Sun and their colleagues devised a double-labeling technique in which they could follow the division of the stem cells, and then observe the trafficking patterns of the early-offspring TA cells. Results from the study "strongly suggest that the bulge stem cell is bipotent, and that the daughter (TA) cells migrate up to the epidermis and down to the root," Lavker said. Added Sun: "There?s been a termendous controversy as to where the epidermal stem cells are. We are proposing that there is just one entity -- an ultimate epidermal stem cell -- located in the bulge area of the hair follicle, that is capable of forming skin or hair." A major "take home message" of the research, Lavker said, is the pivotal importance of the upper follicle in the healing process of the skin: "It places the upper follicle directly in the center, for wound repair." The work also paves the way for designing effective skin cancer treatment, by explaining why skin tumors that are produced for the purpose of research frequently originate in the upper follicle. "In addition to wanting to target stem cells in the bulge for skin cancer (stem cells are the prime source of abnormal growth and mutation), now you also have to consider targeting the upper follicle where the TA cells retain most characteristics of the stem cells," Lavker said. ### Participating with Lavker and Sun in the research were Michael Lehrer, MD., and Pamela Jensen, PhD., of Penn, and Gina Taylor, MD., of Presbyterian Hospital. The work was funded by the National Institutes of Health. Editor?s note: Dr. Lavker may also be reached directly at: 215-898-3232. He will be available for interviews Thursday and Friday, August 10 and 11. Next week he will available all day on Monday and Friday, Aug. 14 and 18, and on the afternoon of Tuesday, Aug. 15. ------------------------------------------------------------------------ Back to EurekAlert! From kottenhahn at icdd.com Fri Aug 25 22:33:34 2000 From: kottenhahn at icdd.com (kottenhahn@icdd.com) Date: Mon Mar 7 05:46:34 2005 Subject: ICDD Grant-in-Aid Increase - International Centre for Diffraction Data Message-ID: <8o66oi$ukh$1@nnrp1.deja.com> International Centre for Diffraction Data Grant-in-Aid > Grant-in-Aid Increase The International Centre for Diffraction Data has increased Grant-in- Aid funding for the 2000-2001 grant cycles in order to allow more grants to be supported. The ICDD® is interested in high quality experimental powder diffraction patterns to add to its internationally renowned database, the Powder Diffraction File (PDF®). The ICDD's Grant-in-Aid program is designed to give limited financial support of those institutions interested in supplying new patterns. A grant can be used most effectively as a supplement to existing funded projects involving the preparation and characterization of new materials, using powder XRD. There are two grant cycles with proposal deadline of: Cycle I - 31 January Cycle II - 31 July For more information, please review the guidelines found on the ICDD web site: http://www.icdd.com/grants/, or contact: Ms. Shelly Wolkov, Grant Coordinator International Centre for Diffraction Data 12 Campus Boulevard Newtown Square, PA 19073-3273 U.S.A. Phone: 610.325.9814 Fax: 610.325.9823 E-mail: wolkov@icdd.com -- Also check out the ICDD's new online shopping - www.icdd.com -- International Centre for Diffraction Data www.icdd.com, www.dxcicdd.com questions? - write webmaster@icdd.com Sent via Deja.com http://www.deja.com/ Before you buy. From wgallin at gpu.srv.ualberta.ca Mon Aug 28 00:12:39 2000 From: wgallin at gpu.srv.ualberta.ca (Warren Gallin) Date: Mon Mar 7 05:46:35 2005 Subject: TX-100 soulubility? References: <8n8cjs$8bi$1@news.online.de> <39A194AF.A902AF56@netinfo.ubc.caX> Message-ID: <39A9F4D7.568FBDC9@gpu.srv.ualberta.ca> Actually, all the connexins are membrane associated; none are cytosolic. The Triton-X-100 solubility assay tells you whether the connexins have been assembled into large gap junctional plaques, which are not TX100 soluble. The newly synthesized and unassembled connexins are soluble in TX100. There are a number of mutants in Cx-32 which are synthesized, do not progress beyond the Er or Golgi, and are better than 95% soluble in TX100 because they are not assembled into plaques in the plasma membrane. "Austin P. So (Hae-Jin)" wrote: > > membrane-associated vs. cytosolic... > > Ulrik wrote: > > > Im a second year student of molecular biology, and have just startet on a > > project about connexins and their assembly into connexons. In my readings I > > find that Triton X-100 soulubility is used to tell something about the > > proteins, but what? > > -- > --- > Austin P. So (Hae Jin) > > I.I.S.G.P. > Biotechnology Laboratory > University of British Columbia > > E-mail: haejin@netinfo.ubc.ca > > http://www.interchange.ubc.ca/haejin/index.html (under construction) -------------- next part -------------- A non-text attachment was scrubbed... Name: wgallin.vcf Type: text/x-vcard Size: 426 bytes Desc: Card for Warren Gallin Url : http://iubio.bio.indiana.edu/bionet/mm/cellbiol/attachments/20000827/a2a1e7eb/wgallin.bin From varunsingh at my-deja.com Mon Aug 28 09:35:48 2000 From: varunsingh at my-deja.com (varunsingh@my-deja.com) Date: Mon Mar 7 05:46:35 2005 Subject: Free Plasmid Drawing Program Message-ID: <8odtc3$8m3$1@nnrp1.deja.com> Have a look at this free plasmid drawing program. http://www.smu.edu/~rsingh Varun Singh Sent via Deja.com http://www.deja.com/ Before you buy. From mwagner at cellbio.emory.edu Mon Aug 28 13:45:36 2000 From: mwagner at cellbio.emory.edu (Mary Wagner) Date: Mon Mar 7 05:46:35 2005 Subject: Axon Instruments digidata 1200 board needed Message-ID: <39AAB350.19D2ED22@cellbio.emory.edu> Hi all... Sorry for the crosspost. We had a computer stolen this weekend that included the board to out Axon Instruments Digidata 1200A. We have custom software and need to replace this board - does anyone have any idea where I can get my hands on a digidata 1200A board? Axon no longer makes the 1200 and has shipped their last 1200AE board. Thanks for any info! Reply here or to my email: mwagner@cellbio.emory.edu Oh, and suggestions on where else to ask would be great! Mary ______________________________________________ Mary Wagner, PhD Department of Pediatrics Emory University, Atlanta GA From sanchezkay at wolfcollective.freeserve.co.uk Tue Aug 29 15:06:08 2000 From: sanchezkay at wolfcollective.freeserve.co.uk ( Adam K) Date: Mon Mar 7 05:46:35 2005 Subject: Biology Message-ID: <8oh5v3$pd3$6@news5.svr.pol.co.uk> I was wondering if you could help me with a project I am doing. I am trying to find out how chemical pollutants (specifically nitrates, ammonia and phosphates) effect pondweed and algae. I am interested in finding out about the different factors which may be effected by the pollutants; factors such as transpiration rate, growth and turgidity of the plants. Please can you tell me how these factors are affected by the pollutants.. or if you could reccommend any webpages I will extremely grateful. Thank you for your time, Sanchez Kay England . From matlas at sciquest.com Tue Aug 29 14:29:23 2000 From: matlas at sciquest.com (SciQuest Auctions) Date: Mon Mar 7 05:46:35 2005 Subject: 377 DNA sequencers availability Message-ID: Come visit SciQuest Auctions . http://www.auctions-sciquest.com We have model 3777 ABI sequencers with 96 well upgrades for less then 40K . All instruments come with new lasers and are still under service contract or call 650-934-0500 From service at communiage.com Tue Aug 29 19:56:56 2000 From: service at communiage.com (service@communiage.com) Date: Mon Mar 7 05:46:35 2005 Subject: =?gb2312?B?u7bTrb34yOvI2rrPzajRtrXE0MK+s73n?= Message-ID: <20000829150501.A14308@yizhi> INXmtcTC8KO/ztK1xEUtbWFpbNKyv8nS1Mq1yrG7r6OhDQrH4cvJ07XT0Mq1yrG1xEUtbWFp bA0KVElDNFBDv8nS1Mq5xOO1xEUtbWFpbMq1yrG7r6Os1rvSqsTj1NrP36OsRS1tYWlsvLS/ ycGivLTK1bW9o6zNrMqxu7nWp7PWzfjJz7y0yrG0q9G4tci24M/uuabE3KOsv+zK1MrUsMmj oQ0KxOPWu9Doz8LU2FRJQzRQQ7KivKS77sTjz9bT0LXERS1tYWlstdjWt7y0v8nP7crcuN/Q p7XEubXNqKGjDQrKtcqxtcTDzs/rwaK8tLPJ1eaho8/W1Nq+zdDQtq+joQ0KDQogICAgICAg ICAgICAgICAgyKXN+NW+v7S/tCBodHRwOi8vd3d3LmNvbW11bmlhZ2UuY29tLmNuDQoNCiAg ICAgICAgICAgICAgICDBory0z8LU2CAgIGh0dHA6Ly93d3cuY29tbXVuaWFnZS5jb20uY24v dGljNHBjMDlhLmV4ZQ0KDQrI57n7xPq21MnPyvbE2sjduNDQy8iko6zO0sPHz/LE+rHtyr6z z9a/tcTQu9Lio6yyor2rzqrE+szhuamzpMbatcS3/s7xoaMNCg0KRGVhciBmcmllbmQsDQoN CkRpZCB5b3Ugd2FudCB0byBlbmpveSBJbnN0YW50IEVtYWlsLCBJbnN0YW50IE1lc3NhZ2Us IEluc3RhbnQgUGFpbnRlci4uLj8gDQoNCkp1c3QgZW5qb3kgdGhlIHdob2xlIG5ldyBUSUM0 UEMgc2VydmljZSBmcm9tIENvbW11bmlBZ2UgTmV0d29yayBUZWNoLiBDby4sIEx0ZC4NCg0K DQogICAgICAgICAgICAgICAgICAgIGh0dHA6Ly93d3cuY29tbXVuaWFnZS5jb20NCiAgICAg ICAgICAgICAg --- From iayork at panix.com Wed Aug 30 14:59:23 2000 From: iayork at panix.com (Ian A. York) Date: Mon Mar 7 05:46:35 2005 Subject: Deoxyglucose and chaperone induction Message-ID: <8ojp2r$9ic$1@news.panix.com> I hypothesized that an effect on cells was caused by upregulation of ER chaperones. Deprivation of glucose and treatment with deoxyglucose (both of which upregulate ER chaperones) caused the effect; but treatement with DTT, which induces the chaperones to a similar extent, does not cause the effect. I see three possibilities: (1) I'm on the wrong track completely. (2) The glucose deprivation/deoxyglucose effect is direct (as, for example, through an effect on glycosylation), rather than via chaperone induction. (3) Glucose deprivation and unfolded proteins induce a slightly different, though overlapping, responses. I'm trying to sort out these possibilities, concentrating on (2) and (3), but I'm having a hard time finding anything useful on the deoxyglucose; while people have used it to induce the unfolded protein response, there doesn't seem to have been anything showing how it works, and whether the responses are in fact the same. On the other hand, there's a lot of literature out there that includes the various keywords, and I'm not kidding myself that I've exhausted it all. Anyone familiar with this stuff? Pointers appreciated. Ian -- Ian York (iayork@panix.com) "-but as he was a York, I am rather inclined to suppose him a very respectable Man." -Jane Austen, The History of England From awiemelt at mail.med.upenn.edu Thu Aug 31 09:58:19 2000 From: awiemelt at mail.med.upenn.edu (Anthony P. Wiemelt) Date: Mon Mar 7 05:46:35 2005 Subject: WANTED: Development Issue Message-ID: <3.0.2.32.20000831105737.007d3640@mail.med.upenn.edu> Would anyone happen to have a copy of Development, 1996, Vol 123 (pgs 1-481) that they wouldn't mind parting with? I study neural patterning in zebrafish, and this is a rather seminal volume to us fish folk. Drop me a line and we can discuss payment. Thanks! Tony ____________________________________ Anthony P. Wiemelt, Ph.D. University of Pennsylvania Department of Cell and Developmental Biology 1211 BRB II/III 421 Curie Blvd. Philadelphia, PA 19104-6058 Telephone: 215-898-9795 Fax: 215-898-9871 ---