Brdu proliferation assay

[dror_seliktar at my-deja.com]

Hi:

I am trying to stain a monolayer of endothelial cells on a collagen
lattice with anti-Brdu monoclonal antibody.  The assay requires a 20
minute incubation with HCL (or microwave) to partially denature the
DNA.  The denaturing step results in a complete destruction of the
collagen substrate and subsequent loose of Endothelial cells.  I was
wondering if there are methods for partial DNA denaturing which are not
destructive to reconstituted collagen.

Thank you,

Dror Seliktar
Georgia Institute of Technology



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