Northern blot for low abundance mRNA
rh at mblab.gla.ac.uk
Tue Mar 7 11:50:34 EST 2000
In article <38C51F2D.70825171 at biocomp.unl.edu>, Chris LaRosa
<clarosa at biocomp.unl.edu> wrote:
> RIGGS wrote:
> > Dear readers,
> > Does anyone have a suggestion for Northern blots for low abundance
> If you cannot see a good signal using total mRNA, then you need to prepare
> fractions enriched for poly A+ mRNA using oligo dT cellulose or one of the
> new matrixes for capturing poly adenylated mrna. Lets assume that you
> have a poly denylated mrna. I have see at times that is is sometimes
> necessary to use up to 20 micrograms (this is a lot) of poly A mRNA in
> order to see low abundance signals. Sometimes it is nearly impossible to
> see the signal by northern blot. More sensitive than the northern blot is
> nuclease protection assays.
Would Slot-Blot work for a low abundance mRNA?
Bob (not offering advice. Just curious)
Centre for Cell Engineering,University of Glasgow,UK.
mail: rh at mblab.gla.ac.uk, Tel: ++44 (0)141 330 4756
Web : http://www.gla.ac.uk/Inter/CellEngineering
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