protein determination is nuclei?

Martin Offterdinger martin.offterdinger at akh-wien.ac.at
Mon Sep 11 09:17:14 EST 2000


John Copier <copier at icrf.icnet.uk> schrieb in im Newsbeitrag:
39B65BBF.19C1CD10 at icrf.icnet.uk...

I suppose that this treatment would probably not destroy the nuclei as they
are pretty stable to hypotonic treatment.
Why not use a stronger dergent that dissassembles the nuclear lamina!
(Disadvantage: release of DNA --> high viscosity)
Or do fractionation into cytoplasm and nucleus by biochemical means.
It largely depends on your desired application!
martin







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