tryptic digestion of dead cells

lisa hunt at dcsun1.epfl.ch
Tue Aug 14 03:13:13 EST 2001


I have found several vague references in the literature* to tryptic
digestion of dead cells.  I would like to know how significant this is
and how long it takes - more specifically, when I routinely trypsinize
my CHO cells, are dead cells disappearing due to tryptic digestion?

*mostly old (Northrup 1926 in J Gen Physiol 9 p 497 or Hoskins 1956 in
Expt Cell Res 11 p 297)

Thanks,
Lisa

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