Phosphorylation

[Leo Serrano]

Hi Everybody,
    I am looking for a protocol to label in vivo a protein, using
phosphate. I do understand this could be extremely nasty and lousy
experiment, but I am looking for details about this. I already establish
a IP protocol that works perfectly for my condictions, and I am just an
easy way to determine whether my protein is phosphorylated... any
suggestion are welcome..!!!
Thanks in advance

LEO