From jbdundas from gmail.com Mon Sep 14 04:43:27 2009 From: jbdundas from gmail.com (jitesh dundas) Date: Mon Sep 14 10:25:16 2009 Subject: [Cell-biology] Request For Help In-Reply-To: <326ea8620909140243v1b0711d7m7ec29c5bd96fa855@mail.gmail.com> References: <326ea8620909131936t4415537el137f7df1c2c257a@mail.gmail.com> <326ea8620909140243v1b0711d7m7ec29c5bd96fa855@mail.gmail.com> Message-ID: <326ea8620909140243m19d810fbt102c8dfe33dace87@mail.gmail.com> Dear Sir/Madam, I am studying the cell organelles in uni-cellular & multi-cellular organisms for their thinking capabilities.I have some tests to perform & I would appreciate if some expert in (cell-biology) could help me in providing experimental data for these tests. The tests are very basic & may have been already done. I open to some suggestions on any new experiments for my research.. Please let me know if you need anything else from my side. Regards, Jitesh Dundas http://jiteshbdundas.blogspot.com From R.Jayakumar from roswellpark.org Mon Sep 14 12:42:24 2009 From: R.Jayakumar from roswellpark.org (Jayakumar, R) Date: Mon Sep 14 15:35:53 2009 Subject: [Cell-biology] Request For Help In-Reply-To: <326ea8620909140243m19d810fbt102c8dfe33dace87@mail.gmail.com> References: <326ea8620909131936t4415537el137f7df1c2c257a@mail.gmail.com> <326ea8620909140243v1b0711d7m7ec29c5bd96fa855@mail.gmail.com>, <326ea8620909140243m19d810fbt102c8dfe33dace87@mail.gmail.com> Message-ID: <5D223E11-DDCA-41A9-86B1-0B8259654B79@mimectl> Dear Jitesh Your question is very vague. you need to be more specific. What is "thinking capabilities" of cell organelles and what kind of organisms are you planning to study? And what are the "tests" or experiments you have in mind? Please elaborate. Jay ________________________________ From: cellbiol-bounces@oat.bio.indiana.edu [cellbiol-bounces@oat.bio.indiana.edu] On Behalf Of jitesh dundas [jbdundas@gmail.com] Sent: Monday, September 14, 2009 5:43 AM To: cellbiol@magpie.bio.indiana.edu Subject: [Cell-biology] Request For Help Dear Sir/Madam, I am studying the cell organelles in uni-cellular & multi-cellular organisms for their thinking capabilities.I have some tests to perform & I would appreciate if some expert in (cell-biology) could help me in providing experimental data for these tests. The tests are very basic & may have been already done. I open to some suggestions on any new experiments for my research.. Please let me know if you need anything else from my side. Regards, Jitesh Dundas http://jiteshbdundas.blogspot.com _______________________________________________ Cellbiol mailing list Cellbiol@net.bio.net http://www.bio.net/biomail/listinfo/cellbiol This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. From R.Jayakumar from roswellpark.org Mon Sep 14 16:26:25 2009 From: R.Jayakumar from roswellpark.org (Jayakumar, R) Date: Mon Sep 14 16:50:14 2009 Subject: [Cell-biology] Request For Help In-Reply-To: <326ea8620909141154i77f2580dr14ac3aa54096f7cf@mail.gmail.com> References: <326ea8620909131936t4415537el137f7df1c2c257a@mail.gmail.com> <326ea8620909140243v1b0711d7m7ec29c5bd96fa855@mail.gmail.com> <326ea8620909140243m19d810fbt102c8dfe33dace87@mail.gmail.com> <5D223E11-DDCA-41A9-86B1-0B8259654B79@mimectl> <326ea8620909141154i77f2580dr14ac3aa54096f7cf@mail.gmail.com> Message-ID: The paper from bell Labs IS very interesting. But from your answer (from the organelles you are interested in), it is clear that you intend to work on eukaryotic cells, but it is still not clear what kind of cells you have in mind, since each eukaryotic cell is a highly specialized "piece of equipment", nor is it clear to what extend you want to take your experiments forward and the expected observation you intend to make. Do you have a hypothesis which you intend to test?? That would help. As you might know, the immune system is replete with similar memory responses, especially related to plasma cells and long term immunity against diseases. I would suggest researching papers on plasma cell longevity, long term immunity versus short lived immunity and memory T-cells in literature. It has been an enigma how plasma cells in the bone marrow recognize and maintain immunity by producing antibodies against certain pathogenic antigens like that of small pox virus, when they don't do the same for other antigens like the cold virus. A clue might be that the small pox virus is dangerous while the cold virus is not. You should proceed in that direction with your experiments.. Two variables in your experiments should be TIME (intervals between exposures) and INTENSITY (concentration and number) of the stimulus. An experiment to study such memory responses in cells should consist of repeated exposure of the cell you have in mind to a stressful stimulus(protein, heat, nutrient deprivation or some other factor towards which the particular cell type or types you are interested in are specialized to respond to), varying the time periods of exposure and the dosage of the stimulant. Studying chromosomal translocations. Please correct me, if I am barking up the wrong tree :-)). Again a hypothetical model which you aim to test, with aims would be useful for people to help you with experiments. Bye Best ofluck Jay dddddddddddddddddddddddddddddddddddddddddddddddddddd Jayakumar R. Nair, Ph.D., HRI Scientist, Dept. of Immunology, Roswell Park Cancer Institute, Elm and Carlton St. Buffalo, NY 14263 Tel: 716-845-8231 email: r.jayakumar@roswellpark.org aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa "Nil magnum nisi bonum" -----Original Message----- From: jitesh dundas [mailto:jbdundas@gmail.com] Sent: Monday, September 14, 2009 2:54 PM To: Jayakumar, R Subject: Re: [Cell-biology] Request For Help Dear Jay, Thank you for your reply. Your question is very vague. Sorry I don't agree with you. I was trying to keep it brief. In fact, you are the first expert who didn't get my question. I guess every person thinks differently. you need to be more specific. Yes, I am provided I get a positive response from an expert like you. Morever, I am cautious of not providing my research paper details to anyone without authentication. ?What is "thinking capabilities" of cell organelles and what kind of organisms are you planning to study? WELL, as humans have brain for thinking & control of major operations.I want to verify/investigate if cells can do the same. I maybe looking at the cell/brain analogy besides studying cell behavior.To what level do cells reflect brain-like behavior.. Few papers (one froma bell laba@pubmed titled -can cells think?) And what are the "tests" or experiments you have in mind? ?Please elaborate. First I just want to study cellular behavior in different conditions like extreme temperature, pathogen infiltration,etc.. Nucleus is my primary organelle of interest besides mitochondria, edoplasmic reticulum,etc. I am looking at all possible ways to find if cells can reproduce creative thinking capabilities. I hope this helps. Please suggest computational tools and wet experiments suitable for this hypothesis. Regards, Jitesh Dundas On 9/14/09, Jayakumar, R wrote: > Dear Jitesh > Your question is very vague. you need to be more specific. What > is "thinking capabilities" of cell organelles and what kind of > organisms are you planning to study? And what are the "tests" or > experiments you have in mind? Please elaborate. > > Jay > > > ________________________________ > From: cellbiol-bounces@oat.bio.indiana.edu > [cellbiol-bounces@oat.bio.indiana.edu] On Behalf Of jitesh dundas > [jbdundas@gmail.com] > Sent: Monday, September 14, 2009 5:43 AM > To: cellbiol@magpie.bio.indiana.edu > Subject: [Cell-biology] Request For Help > > Dear Sir/Madam, > > I am studying the cell organelles in uni-cellular & multi-cellular > organisms for their thinking capabilities.I have some tests to perform > & I would appreciate if some expert in (cell-biology) could help me in > providing experimental data for these tests. > > The tests are very basic & may have been already done. I open to some > suggestions on any new experiments for my research.. > > Please let me know if you need anything else from my side. > > Regards, > Jitesh Dundas > http://jiteshbdundas.blogspot.com > _______________________________________________ > Cellbiol mailing list > Cellbiol@net.bio.net > http://www.bio.net/biomail/listinfo/cellbiol > > > This email message may contain legally privileged and/or confidential > information. If you are not the intended recipient(s), or the > employee or agent responsible for the delivery of this message to the > intended recipient(s), you are hereby notified that any disclosure, > copying, distribution, or use of this email message is prohibited. If > you have received this message in error, please notify the sender > immediately by e-mail and delete this email message from your computer. Thank you. > This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. From zamtul from uwo.ca Mon Sep 21 13:01:42 2009 From: zamtul from uwo.ca (Zareen Amtul) Date: Mon Sep 21 13:02:35 2009 Subject: [Cell-biology] WS1 Message-ID: Hi Marie, Do you still have the problem with these cells. Did you buy it from ATCC. Is it CRL-2029? Thanks. Zareen [Cell-biology] WS1 cells Kirchner, Marieluise Marieluise_Kirchner at hms.harvard.edu Tue May 16 18:03:16 EST 2006 Previous message: [Cell-biology] PC12 Next message: [Cell-biology] Re: Research inquiry Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] I received recently WS1 human fibroblast cells and I am trying to culture them. But it seems that they are not growing very well (close to "not at all") in my hands. Does maybe somebody has some experiences with this cell line and can give me some suggestions how to culture them? That would be very helpful. Thanks Marie -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/cellbiol/attachments/20060516/cab17cda/attachment.html Previous message: [Cell-biology] PC12 Next message: [Cell-biology] Re: Research inquiry Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] More information about the Cellbiol mailing list From Heather.Vincent from manchester.ac.uk Tue Sep 22 01:47:15 2009 From: Heather.Vincent from manchester.ac.uk (Heather Vincent) Date: Tue Sep 22 08:04:25 2009 Subject: [Cell-biology] Distance courses in bioinformatics and systems biology Message-ID: <4AB872F3.6060803@manchester.ac.uk> At the University of Manchester, UK, we have over 10 years experience in providing distance courses, at Masters level, to participants from a range of backgrounds, including Biology, Healthcare, Medicine and Computer Science. These courses are designed to enable biologists and computer scientists to update their skills while remaining in the workplace. Our distance courses are also very suitable for home study, helping you to keep in touch with a research environment while taking a career break. Participants continuing to Masters level will complete a research project. The courses beginning on 5 October 2009 include: - Introduction to Bioinformatics (http://octette.cs.man.ac.uk/bioinformatics/modules/bs61061.html) - Bioinformatics for Systems Biology (http://octette.cs.man.ac.uk/bioinformatics/modules/BIOL61820.html) You will find further information, including fees and a link to the online application form, here: http://octette.cs.man.ac.uk/bioinformatics/index.html If you have any questions, or need advice on the course options, please contact Heather.Vincent@manchester.ac.uk From Robin.Haw from oicr.on.ca Tue Sep 22 16:04:43 2009 From: Robin.Haw from oicr.on.ca (Robin Haw) Date: Wed Sep 23 10:00:22 2009 Subject: [Cell-biology] Reactome Pathway Database User Survey Message-ID: Reactome is committed to providing access to high-quality pathway information and helpful data analysis tools. With this in mind, we are actively soliciting comments from the research community in order to assess community needs. We are interested to hear about your experience with Reactome, and would like to know a bit about your background and research interests so that we can continue to improve the Reactome site and tools. You can access the survey at: http://tinyurl.com/l48zzq Thank you for taking part. Robin Haw Manager of Reactome Outreach Outreach [at] reactome.org http://www.reactome.org From vbaranet from hawaii.edu Wed Sep 23 17:04:33 2009 From: vbaranet from hawaii.edu (Valeria Baranets) Date: Wed Sep 23 21:54:56 2009 Subject: [Cell-biology] sent from wrong email address- UV sterilization??? Message-ID: <9fe48d230909231504o711ea194q8494bf882ee5bf25@mail.gmail.com> Hi Jens, I saw your post about the CL-1000 crosslinker. I have one myself in the lab and am trying to figure out how long my nylon membrane needs to sit inside the crosslinker for the DNA to hybridize. You said the setting is 120,000 microjoules per cm2 but for how long? Thanks, Valery