From linhnt13 from gmail.com Mon Nov 3 10:08:30 2008 From: linhnt13 from gmail.com (Linh Ngo) Date: Mon Nov 3 12:35:07 2008 Subject: [Cell-biology] heteromerization Message-ID: <6d7a7a100811030708v50b6920ck9a773e0cb6349158@mail.gmail.com> Hello, I am an undergraduate student studying Plant Physiology. I read in my textbook about aquaporin and it says aquaporins are regulated by heteromerization. I have searched on the web but there is no info about this. Could anyone here explain me what heteromerization means? Thank you very much -- Sincerely LinhNT From info from noster-it.com Wed Nov 5 05:10:51 2008 From: info from noster-it.com (Dokorek) Date: Wed Nov 5 10:44:57 2008 Subject: [Cell-biology] who would like maintain "cell group" in biospace Message-ID: and help our students. It is simply http://biospace.ethz.ch From alice508 from u.washington.edu Thu Nov 20 17:21:44 2008 From: alice508 from u.washington.edu (alice508@u.washington.edu) Date: Thu Nov 20 22:43:48 2008 Subject: [Cell-biology] pH adjustments Message-ID: I would appreciate any suggestions for help with adjusting the pH of my media or working solution. I am adding BSA to study the effects on my conditionally immortalized cells grown in RPMI media with 2% FBS and have had difficulties with pH adjustment. I initially tried adding the BSA to media to make a working solution then adding it to my plates, but the amount of working solution is quite significant and causes the media to immediately turn yellow. I then tried to adjust the pH of the working solution with sodium bicarbonate, but the amount needed was about 1:1 and diluted the media too much. I then tried making the working solution with sterile water, then using 1N NaOH to adjust the pH to about 7.6 (which is the pH of the media) and the amount needed was acceptable. However, when I placed this media into 37 degrees incubator with 5% C02, at 24 hours, the pH of this changed to orange/yellow and the pH dropped to 6.8. Any suggestions on how to get the pH within physiological range accounting for the C02 and temperature changes as well as amount needed to get the pH right without diluting the media? Thank you! From info from noster-it.com Sun Nov 30 03:42:28 2008 From: info from noster-it.com (Dokorek) Date: Sun Nov 30 13:35:17 2008 Subject: [Cell-biology] Mitosis Gets Harder Thanks To New Gene Discovery Message-ID: <962a50a2-f8b8-46fd-9922-e7125007e19d@o2g2000yqd.googlegroups.com> A biological process taught to every student studying biology has just become a little more complicated thanks to a new discovery. Scientists from the University of Bath have found that a protein called RASSF7 is essential for mitosis, the process by which a cell divides in two. In research published in the journal Molecular Biology of the Cell, the scientists have shown that the protein is essential for building the microtubules that allow the two halves of the cell to slide apart. =93What makes mitosis so interesting is that it is one of the biological processes that everyone remembers from their days at school,=94 said Dr Andrew Chalmers from the University=92s Department of Biology & Biochemistry. Dokorek -------------- Portal to share biological information-data between people http://biospace.ethz.ch