From lxbking from gmail.com Mon Jun 1 14:17:21 2009 From: lxbking from gmail.com (Xiaobo Li) Date: Mon Jun 1 17:45:52 2009 Subject: [Chlamydomonas] about the fosmid Message-ID: <5508ee9e0906011217x419abff4ga6a2d179f80ef8c9@mail.gmail.com> Hi All, I am a new person to the Chlamy community. Recently I want to clone a genomic fragment of about 7kb. Considering the size, PCR maybe a challenge. So I want to order the fosmid covering it and hopefully can obtain the fragment by digestion. Anyone knows where to order the fosmid? Thank you very much. Xiaobo -- ************************************* Xiaobo Li Graduate Assistant DOE Plant Research Laboratory Department of Plant Biology Michigan State University East Lansing, MI 48824 ************************************* From william.snell from utsouthwestern.edu Mon Jun 1 18:31:57 2009 From: william.snell from utsouthwestern.edu (William J. Snell) Date: Mon Jun 1 19:28:50 2009 Subject: [Chlamydomonas] about the fosmid In-Reply-To: <5508ee9e0906011217x419abff4ga6a2d179f80ef8c9@mail.gmail.com> References: <5508ee9e0906011217x419abff4ga6a2d179f80ef8c9@mail.gmail.com> Message-ID: Hi, Xiaobo: Once you get the BAC, if you are interested in a learning about a BAC Retrieval method that we learned about from a colleague in the fly community, please let us know. The method uses recombineering in a cell that harbors a BAC to retrieve any fragment of DNA up to 12-20kb independently of location of restriction sites. ~Bill Snell On Jun 1, 2009, at 2:17 PM, Xiaobo Li wrote: > Hi All, > > I am a new person to the Chlamy community. Recently I want to clone a > genomic fragment of about 7kb. Considering the size, PCR maybe a > challenge. > So I want to order the fosmid covering it and hopefully can obtain the > fragment by digestion. Anyone knows where to order the fosmid? Thank > you > very much. > > Xiaobo > > -- > ************************************* > Xiaobo Li > Graduate Assistant > DOE Plant Research Laboratory > Department of Plant Biology > Michigan State University > East Lansing, MI 48824 > ************************************* > _______________________________________________ > Chlamy mailing list > Chlamy@net.bio.net > http://www.bio.net/biomail/listinfo/chlamy William J. Snell, Ph.D. Department of Cell Biology, University of Texas Southwestern Medical School Rm. K2-226 5323 Harry Hines Blvd. Dallas, TX 75390-9039 T-214-648-2332 FAX-214-648-8694 Email:william.snell@utsouthwestern.edu From prikrylova from orbit.umbr.cas.cz Tue Jun 30 08:20:11 2009 From: prikrylova from orbit.umbr.cas.cz (Pavla =?iso-8859-2?Q?P=F8ikrylov=E1?=) Date: Tue Jun 30 09:00:45 2009 Subject: [Chlamydomonas] is autolysin comercially available? Message-ID: <74c6cd79297288118cff3cdcb23ee42a.squirrel@wmail.umbr.cas.cz> Dear all, could anyone please tell me if there is a possibility to buy g-autolysin? With many thanks, Pavla Prikrylova