From chlamy from duke.edu Fri Apr 4 13:25:16 2008 From: chlamy from duke.edu (Elizabeth Harris) Date: Fri Apr 4 13:49:16 2008 Subject: [Chlamydomonas] cover illustrations for the new Chlamydomonas Sourcebook Message-ID: As you're probably aware, a new edition of The Chlamydomonas Sourcebook is currently in production. We are looking for photographs or drawings suitable for the covers of each of the three volumes. These illustrations can be in full color. Our editors at Elsevier would like to have the final illustrations in hand by April 18th, so we need to work quickly. If you have images that you think might be suitable, please send low-resolution .jpg files for review to Elizabeth Harris, chlamy@duke.edu. The three volumes, with their working subtitles, are as follows: Volume 1. Introduction to Chlamydomonas and Its Laboratory Use (by Elizabeth Harris) Suitable illustrations might include light micrographs or EMs of individual cells or gametes pairing; we're open to all possibilities here Volume 2. Organellar and Metabolic Processes (edited by David Stern) For this one, perhaps something on the "green" side of Chlamy? Roughly half of this volume is concerned with various aspects of metabolism, and half with photosynthesis, respiration, and biogenesis of chloroplast and mitochondria. Volume 3. Cell Motility and Behavior (edited by George Witman) Here we'd like to see an immunofluorescence image of the cell, an EM of the flagellum/basal body, or perhaps a line drawing illustrating IFT or some other process or structure. Thanks in advance for your interest and help! -- Elizabeth H. Harris Chlamydomonas Center http://www.chlamy.org/ From jackie20008 from gmail.com Thu Apr 17 19:52:52 2008 From: jackie20008 from gmail.com (lanyu lan) Date: Thu Apr 17 20:38:00 2008 Subject: [Chlamydomonas] Information on culturing Chlorella Message-ID: <1d4c5a100804171752x494f6c6ch420b74d8409d5735@mail.gmail.com> Hello, this is a Chinese girl that need your help. would you please provide me some information about the Chlorella? the culture media, growing conditions, maintenance and protocols for pigment analysis (e.g. Carotenoids, e.g. xanthophyls). I see that on your web.I want to do some research about it, but could not find enough information. Thanks for you help. Regards Yours, Jackie From s.kiaulehn from web.de Mon Apr 28 10:33:21 2008 From: s.kiaulehn from web.de (s.kiaulehn@web.de) Date: Mon Apr 28 10:47:54 2008 Subject: [Chlamydomonas] Need help to find a working mithramycin staining protocol for Chlamydomonas Message-ID: <10e19030-e030-4327-9100-003a9afd80c5@w74g2000hsh.googlegroups.com> Dear Chlamydomonas community, I am PhD student working on Chlamydomonas reinhardtii and tried to stain cw15 (mt-) cells with mithramycin, a dye for the nucleus I found in the Chlamydomonas Source Book. I referred Bridget & Whatley (1975) to strain the cells by adding 20 ?g/ml mithramycin and 5 mM MgCl2 (final concentration). I incubated the cells 20 min at room temperature and in the absents of light before I watched them through the microscope (Zeiss Axiophot, 450-490 nm excitation, 510 nm beam splitter, 520 nm barrier filter). Unfortunately I was not successful staining the cells. I only could see strong red auto-fluorescent of chloroplasts but no yellow/green fluorescent nucleus. Is there anybody out there knowing a working mithramycin staining protocol for Chlamydomonas who is willing inaugurate me into the secret of staining cells with mithramycin? Many thanks in advance! Kind regards Stefanie