[Chlamydomonas] Frameshift mutagens

Marilyn Kobayashi via chlamy%40net.bio.net (by kobayash from nature.berkeley.edu)
Thu May 8 18:46:44 EST 2008


We are developing a reverse genetics strategy for finding mutations in 
specific genes. Presently we are using uv mutagenesis but would like to 
try other mutagens to get a broader spectrum of mutants. We have tried a 
frameshift mutagen, ICR-191. Does anyone have any experience to maximize 
the mutation rate using ICR-191? Is there other frameshift mutagens we 
could try with chlamy?

Thanks,

Marilyn

-- 
<>-<>-<>-<>-<>-<>-<>-<>-<>-
Marilyn Kobayashi
Niyogi Lab
441 Koshland Hall
Plant and Microbial Biology
University of California
Berkeley, CA 94720
510-643-6604



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