At a glance, this seems to be pretty interesting,but the end-point quantification
of PCR products cannot always bring to the estimation of a starting template
number, principally, because the PCR kinetics is not on the exponential
amplification schema, which is usually believed.
If you would be interested in this area, I am happy to show you my article,
ANALYTICAL BIOCHEMISTRY 229,207-213,1995.
Takahiko ISHIGURO, Ph.D.
Tokyo Research Laboratories
E-mail:isiguro at tosoh.co.jp