> I'm presently doing some comparisons between different buffers to
> dilute my labelled antibody in, for use in ELISA. So far I have established
> sofar is that the standard conjugate buffer that we use here (2% PVP,
> 0.2% Ovalbumin in PBS-T) can be improved upon with the addition of
> various other proteins such as BSA and Casein.
>What I am after is are buffers that will increase the signal : noise ratio
>in the actual ELISA. My actual Alkaline Phosphatase conjugates Pabs or mabs are
>quite stable enough. I don't really have any problems, I'm just trying
>to increase the sensitivity of the ELISA.
My comment is: Have you tried the NAD/H amplification system for AP-conjugates.
My own experience with it isn't the best - the system was too sensitive when
it came to background noice but I know about people in Wageningen who has
had good experience with it for detection of plant pathogens.
Dynal Microbiology R&D
E.mail: vigfrid.ness at veths.no