I am a new subscriber in this newsgroup, and this is the first E-mail.My
study about the molecular characterization of the root-knot nematodes
(Meloidogyne).I have just started my study, but there is some problem
with me to extract the DNA from the nematodes so:
if any one have an easy method to this extraction please send
it to me.
, and if any one faced him some problem with extracting DNA and
He found the solutions. it may be useful to me if he can send it
also I have some questions:
Can I use Polyvenylpyrollydone(PVP M.W. 360 000) instead of
Polyvenylpyrollidone (PVPP) to prepare a buffer to extract DNA [it
consists of EDTA, Sarcosyl, PVPP(water insoluble)?
How could I sterilize the PVP, and Sarcosyl, could I autoclaved it?
SORRY for this too long E-mail, but all of these questions is very
important to me.Thanks for your cooperation.
Abd Elnasser Elashry
Agricultural genetic Engineering Research Institute(AGERI)
Agricultural Research Center(ARC)
9 Gamaa st., Giza, Egypt
E-mail: idl at ageri.sci.eg