On 16 Feb 96 at 1:23, Larry Winger wrote:
> In our most recent HRP conjugation, however, we had terrible coupling,
> compared to a previous Genosys kit with the same antibody -- we didn't know
> whether or not that was the fault of the kit, or our own problem, but it
> does look worrisome -- we'll see how the second coupling (each kit comes
> with nominal reagents for two coupling procedures) goes.
>We use the kit to conjugate our monoclonal lines, reducing one
potential source of variation. We are certain that
the kit is at the root of the problem.
> It would be interesting to hear from a Genosys representative in this
> regard, who can reassure us on relevant QC questions!
>
I agree. If anybody can supply an email address I'll invite them to
comment.
> Larry 'wearing three hats in these cold times' Winger
>Not THE Larry Winger, the author?
Kevin
Dr Kevin O'Donnell "Work as if you were in the early days
Diagnostics and Molecular Biology of a better nation"
SASA - Alasdair Gray
Edinburgh
