I am working on stools for RT-PCR of Norwalk viruses.
I have tried four different methods of RNA extraction :
1. Boom's method
3. Promega's RNA extraction kit and
4. Spin cartridge from Gibco.
However, the results are not satisfactory. I don't know if there
are any problems caused due to the extraction methods or because of
the quantity of the related virus was too low.
Can anyone give me advice on how to solve the above problems?