IUBio

DNA isolation from fungal spores

Douglas Rhoads drhoads at comp.uark.edu
Tue Sep 15 09:11:38 EST 1998


We had pretty good luck just boiling spore suspensions in TE.  We got 
good sensitivity down to somewhere around 4-5 spores/PCR for 
Magneporthe grisea.


On 15 Sep 98 at 12:09, Kevin O'Donnell wrote:

> Can anyone suggest a good (i.e. rapid, simple) method for isolating 
> DNA from fungal spores? The quality of DNA has only  to be good enough for 
> PCR or LCR ampliciation, rather than cloning, orsequencing.
> 
> Replies to the list please, rather than private email.
> 
> Thanks,
> 
> Kevin
> Dr Kevin O'Donnell                          
> Diagnostics and Molecular Biology    
> Scottish Agricultural Science Agency   Tel 0131 244 8924
> Edinburgh                                              Fax  0131 244 8926
> EH12 8NJ   UK
> 
> 

PLEASE NOTE THAT MY EMAIL HAS CHANGED TO DRHOADS at COMP.UARK.EDU

++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
==========================================================    
  "Strong and Bitter Words Indicate a Weak Cause."      
                From a Chinese Fortune Cookie         
==========================================================
Douglas Rhoads,  Assc. Professor & Chair                
Dept. of Biological Sciences, University of Arkansas
drhoads at comp.uark.edu, http://comp.uark.edu/~drhoads    
501-575-3251




More information about the Diagnost mailing list

Send comments to us at biosci-help [At] net.bio.net