Could the epitopes be overlapping?
<daemon at hgmp.mrc.ac.uk [mailto:daemon at hgmp.mrc.ac.uk] On Behalf Of>
message news:88gdui$ipg$1 at mercury.hgmp.mrc.ac.uk...
>>>> -----Original Message-----
> From: Robert Burns [mailto:burns at sasa.gov.uk]
> Sent: 16 February 2000 11:36
> To: diagnost at net.bio.net> Subject: Antibody interactions
>>> We have come across a curious phenomenon when making cocktails of
> monoclonals. Two of our mab/Alk.phos conjugates which detect Potato
> Virus A
> pick up an isolate of this virus individually in ELISA. However when a
> mixture of the two conjugates is used we cannot detect this isolate.
> ideas of what is going on?
>>> Robert Burns
>>>> Antibody Unit
> Craigs Road
> EH12 8NJ
>>burns at sasa.gov.uk>>> ---