From Alan.Wheeler at gov.ky Tue Aug 20 04:19:49 2002 From: Alan.Wheeler at gov.ky (Wheeler, Alan) Date: Mon Mar 7 06:30:59 2005 Subject: Question about agarose gel electrophoresis Message-ID: <4F3534137955D3119C2400508B0EC60A07353FEE@SVREXCH> I am just starting to monitor for West Nile Virus using PCR. I expect to be getting a large number of negative samples. I am wondering if it is possible to re-use a gel if all the samples run in it come up negative. What I was thinking of doing would be to prepare 24 samples and run them three times in the same gel. Please send any replies directly to me because I have not yet signed up to this group. regards Alan Disclaimer The contents of this e-mail are confidential to the addressee and are intended solely for their exclusive use. If you are not the addressee, you have received this e-mail in error and any disclosure, copying, distribution or action taken in reliance on it is prohibited and may be unlawful. Any opinions expressed in this e-mail are those of the author and do not necessarily reflect those of the Cayman Islands Government or anyone associated thereof. Visit the official website of the Cayman Islands Government at http://www.gov.ky -------------- next part -------------- An HTML attachment was scrubbed... URL: http://iubio.bio.indiana.edu/bionet/mm/diagnost/attachments/20020820/85d33077/attachment.html From padmaja at uic.edu Tue Aug 20 04:20:23 2002 From: padmaja at uic.edu (Padmaja Vellanki) Date: Mon Mar 7 06:30:59 2005 Subject: mycoplasma contamination - protein expresssion Message-ID: Can anyone explain how mycoplasma contamination can affect protein expression in Eukaryotic system?? Also, suggest me the best Eukaryotic system for recombinant protein production in large scale. thaks padmaja