Tell me how to prepare the 25kb plasmid DNA


Sat Nov 4 15:04:32 EST 1995


   Hello, 
   My name is Dongkook Park, graduate student of Seoul National
Student and I'm studying the molecular genetics of Drosphila
melanogaster.
   BTW, I have the obstacle to prepare the transposon plasmid for
transformation.
  I have subcloned 15kb DNA fragment at the pCaSpeR vector.
  So, I confirmed the clone through  mini prep.
 But, I does not  prepare the more amount of it by medium prep.

  Please, mail me how to prepare the large size DNA prep 
  whoever knows the tip.
  
  My address is followed;
     dkpark at power4.snu.ac.kr







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