Giemsa staining after Drosophila polytene chromosome in situ hybridization
mbthomas at ccvax.sinica.edu.tw
Mon Nov 18 14:20:19 EST 1996
In our lab we perform Giemsa staingin after polytene chromosome in situ
hybridization. Somehow Giemsa staining does not work at all.
We followed Todd Lavery's protocol of Rubin's Lab Manual. Some modification we
made, the labelling DNA we use DIG system instead of biotin system and we use
Alkaline Phosophatase conjugated anit-DIG anitbody from BM instead of Detek
After in situ hybridization we gain good sigal(s) but when weperformed Giemsa
staing we get nothing.
Does anybody have any openion, suggestion or possible reason for such kind of
result? Why we just don't have Giemsa banding pattern after in situ?
Reply me to mbthomas at ccvax.sinica.edu.tw
Institute of Molecular Biology
e-mail: mbthomas at ccvax.sinica.edu.tw
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