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dead flies

John Roote j.roote at gen.cam.ac.uk
Thu Feb 3 12:13:15 EST 2000

A number of labs in the UK (Cambridge and London) have reported incidents of
presumed toxic media.  The common symptoms are:
     1)    some crosses or stocks die suddenly and completely, flies die
before egg-laying
     2)    normal egg-laying, but embryos die (go brown)
     3)    some embryos die, then all seems fine until 1st/2nd instar larvae

Many stocks recover - eggs laid later develop OK, but the culture is
consequently delayed.  Flies transferred from sickly cultures often recover.
These symptoms are very inconsistent, adjacent vials showing completely
different levels of 'toxicity'.
One lab has reported that these symptoms occur on media made with cornmeal,
but not wheat flour - all other ingredients being identical.

I would be very interested to hear of similar experiences elsewhere in the fly

John Roote

Michael Ashburner's lab                                   Tel: 44 1223 333982
Department of Genetics                                     Fax: 44 1223 333992

University of Cambridge                                   email:
jr32 at mole.bio.cam.ac.uk
Downing Street

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