From jeedward from yahoo.com Wed Feb 6 10:35:42 2008 From: jeedward from yahoo.com (John Edward) Date: Wed Feb 6 10:55:34 2008 Subject: [Drosophila] Draft paper submission deadline is extended: BCBGC-08 Message-ID: <204134.63729.qm@web45901.mail.sp1.yahoo.com> Dear Professors, Colleagues and Friends Kindly share the announcement below with those who may be interested: thank you in advance. Sincerely John Edward BCBGC-08 Draft paper submission deadline is extended. The 2008 International Conference on Bioinformatics, Computational Biology, Genomics and Chemoinformatics (BCBGC-08) (website: www.PromoteResearch.org ) will be held during July 7-10 2008 in Orlando, FL, USA. The draft paper submission deadline is extended until February 19 2008 due to several requests from the authors. The conference will be held at the same time and location where several major events (see below) will be taking place. BCBGC brings together both academic and industrial scientists and developers from a diverse range of disciplines including bioinformatics, computer science, computational biology, genomics, proteomics and chemoinformatics. One of the main goals of the conference is to promote the dissemination of research to a multidisciplinary audience and to facilitate communication among researchers in different fields. Papers that demonstrate applications of existing techniques or developments of new methods are equally welcomed to the conference. Sincerely John Edward ? International Conference on Artificial Intelligence and Pattern Recognition (AIPR-08) ? International Conference on Automation, Robotics and Control Systems (ARCS-08) ? International Conference on Bioinformatics, Computational Biology, Genomics and Chemoinformatics (BCBGC-08) ? International Conference on Enterprise Information Systems and Web Technologies (EISWT-08) ? International Conference on High Performance Computing, Networking and Communication Systems (HPCNCS-08) ? International Conference on Software Engineering Theory and Practice (SETP-08) ? International Conference on Theoretical and Mathematical Foundations of Computer Science (TMFCS-08) --------------------------------- Never miss a thing. Make Yahoo your homepage. From hilde from flymine.org Fri Feb 8 06:13:59 2008 From: hilde from flymine.org (Hilde Janssens) Date: Fri Feb 8 08:28:49 2008 Subject: [Drosophila] FlyMine version 11.0 released Message-ID: <47AC3977.3080702@flymine.org> We have released version 11.0 of FlyMine. The D. melanogaster genome has been updated to release 5.5 from FlyBase, expression data related to Tiffin motifs have been added and many other data sources are updated. We have also added new widgets and improved menus on results and list analysis pages. We have changed the way we present orthologue data from InParanoid to make it simpler and more obvious. Orthologues and Paralogues now appear in a single Homologue class which has a type of 'orthologue' or 'inParalogue', we no longer use the terms 'main' and 'secondary'. We have also added an orthologue conversion section to analysis pages of gene lists. For a list of all changes see http://www.flymine.org/release-notes.shtml For a full list of data sources see www.flymine.org/release-11.0/dataCategories.do For all types of feedback email support@flymine.org The FlyMine Team - About FlyMine - FlyMine is an integrated database of genomic, expression and protein data from Drosophila, Anopheles and C. elegans. As an integrated resource it is possible to run data mining queries that span domains of biological knowledge. FlyMine is a freely available resource funded by The Wellcome Trust. ----------------- From hilde from flymine.org Fri Feb 8 06:18:09 2008 From: hilde from flymine.org (Hilde Janssens) Date: Fri Feb 8 08:29:10 2008 Subject: [Drosophila] FlyMine Grant Renewal - we need your input! Message-ID: <47AC3A71.6080907@flymine.org> FlyMine's grant renewal is soon due and therefore we ask for your help: We would appreciate your taking the time to complete a short survey on what you expect from FlyMine and how we could improve FlyMine to better suit the needs of our current and future users. The survey is accessible from the top menu bar of every FlyMine page and consists of 10 questions only. Thank you for your help! The FlyMine Team http://www.flymine.org support@flymine.org - About FlyMine - FlyMine is an integrated database of genomic, expression and protein data from Drosophila, Anopheles and C. elegans. As an integrated resource it is possible to run data mining queries that span domains of biological knowledge. FlyMine is a freely available resource funded by The Wellcome Trust. ----------------- From donito from woh.rr.com Thu Feb 7 19:31:48 2008 From: donito from woh.rr.com (Don Shuff) Date: Fri Feb 8 15:11:18 2008 Subject: [Drosophila] Caltech media recipe Message-ID: <20080207193148.085946e2.donito@woh.rr.com> Would someone please be so kind as to provide the Caltech media recipe/procedure? I am having a hard time finding the book by Ashburner that describes the recipe. It appears to be out of print. Thank you, Don From cpinzone from mail.ucf.edu Sun Feb 10 19:37:38 2008 From: cpinzone from mail.ucf.edu (Cheryl Pinzone) Date: Mon Feb 11 09:45:59 2008 Subject: [Drosophila] SE Ecology & Evolution Conference Message-ID: <47AF5200.E922.000A.0@mail.ucf.edu> 2008 SOUTHEASTERN ECOLOGY AND EVOLUTION CONFERENCE Florida State University Call For Abstracts Florida State University will host the 2008 Southeastern Ecology and Evolution Conference and is announcing a call for abstracts for presentations of research. This is a scientific symposium aimed at graduate, post-doctoral, and undergraduate researchers in the fields of ecology, systematics, and evolutionary biology. The conference will be held on the campus of Florida State University on March 28-30, 2008. Abstract Submission Information Researchers interested in presenting posters or 15-minute talks are invited to submit abstracts pertaining to: Animal Behavior Conservation Ecology Evolution Functional Morphology History and Philosophy of Science Systematics The deadline for the early registration and submission of abstracts is Friday, February 29, 2008. The final date to submit abstracts is Friday March 14, 2008 at 5:00 PM, EST. Please submit your abstract along with registration at the SEEC website, found at http://bio.fsu.edu/~eerdg/seec/ Abstracts should be limited to 250 words, and please include any primary and co-authors as well as session preference. Information about poster and talk requirements is available on the SEEC website. Early registration fees for the meeting are $15; late registration fees are $35. Please contact David McNutt dmcnutt@bio.fsu.edu for questions regarding registration or abstract submission, or general questions about SEEC to Amanda Buchanan at abuchanan@bio.fsu.edu or Eric Jones at ejones@bio.fsu.edu From szimmerm from andrew.cmu.edu Mon Feb 11 12:33:58 2008 From: szimmerm from andrew.cmu.edu (Sandra Zimmerman) Date: Mon Feb 11 13:33:27 2008 Subject: [Drosophila] Removing the peripodial epithelium Message-ID: <47B08706.4010809@andrew.cmu.edu> Does anyone know the best way to remove the peripodial epithelium from wing discs? Do you just tear it off with your forceps? Is it better to do it before or after fixing the tissue? Thanks. Sandra Zimmerman From dmerrill from k-state.edu Tue Feb 19 12:24:31 2008 From: dmerrill from k-state.edu (Arthropod Genomics) Date: Tue Feb 19 12:42:55 2008 Subject: [Drosophila] 2/29 Deadline-Poster Abstracts&Early Registration for April Arthropod Symposium Message-ID: <00a101c8731c$4a068940$78738281@ECOGENOFFICE> SYMPOSIUM: NEW INSIGHTS FROM ARTHROPOD GENOMES April 11-13, 2008, in Kansas City This is a friendly reminder that Friday, February 29, is an important deadline for submitting a poster abstract for presentation at the 2nd Annual Arthropod Genomics Symposium. Abstract Guidelines can be found at www.ksu.edu/agc/symposium.shtml . February 29 is also the deadline to receive discounted early registration fees. After February 29, we will continue to welcome your registration to attend the Symposium at a slightly higher cost. A brochure and complete information with links for registration and hotel reservations can be downloaded at www.ksu.edu/agc/symposium.shtml. SYMPOSIUM PROGRAM: The main symposium sessions will take place on Friday-Saturday, April 11-12. Speakers will present new insights from genomic approaches in arthropods and describe the development of tools for genomic analysis. Optional workshops are scheduled for Thursday and Friday evenings. An evening of jazz and KC barbeque is planned for Saturday night. On Sunday morning, participate in a roundtable discussion with the ArthropodBase Consortium. Activities will conclude by noon on Sunday. FEATURED SPEAKERS: John Kenneth Colbourne, Indiana University, Preservation, expansion and invention of crustacean genes with reference to insect genomes. Christine G. Elsik, Georgetown University, Unusual base composition of the honey bee genome. Sarjeet S. Gill, University of California, Riverside, Mosquito midgut interactions with bacterial toxins. Catherine A. Hill, Purdue University, Tick genome organization and evolution. Thomas Kaufman, Indiana University, The latest news from CNN: What the 12 sequenced Drosophila genomes have told us about rapidly evolving genes and positive selection. J. Robert Manak, University of Iowa, Empirical annotation of arthropod genomes using tiled genomic microarrays. Subbaratnam Muthukrishnan, Kansas State University, Functional genomics of insect chitin metabolism. Hugh M. Robertson, University of Illinois at Urbana-Champaign, What we've learned about the insect chemoreceptors from arthropod genome projects. Bruce R. Schatz, University of Illinois at Urbana-Champaign, BeeSpace: Interactive functional analysis of arthropod genomic data. Jeff Stuart, Purdue University, Avirulence, sex determination, and a physical map of the Hessian fly genome. Judy Willis, University of Georgia, Insect cuticular proteins: Annotation, proteomics, expression, evolution. Evgeny Zdobnov, University of Geneva, Medical School, Switzerland, A comparative perspective on insect genomes. POSTER SESSIONS: There will be two poster sessions, limited to first 150 abstracts received before Friday, February 29. A few platform presentations will be chosen from submitted poster abstracts. WORKSHOPS AND ROUNDTABLE DISCUSSION: On Thursday evening, a workshop on "Community Contributions to Genome Annotation" will feature a presentation on use of the Apollo Genome Annotation Curation Tool by Dr. Chris Elsik (BeeBase). On Friday evening, Dave Clements (NESCent) and Scott Cain (CSHL) will lead a workshop, "Chado Databases and Integration with GMOD Tools." Throughout the meeting, arthropod genome database and bioinformatics tool developers will be available for individual training. On Sunday morning, participate in a roundtable discussion led by members of the ArthropodBase Consortium regarding the generation of integrated arthropod genome databases and tools for genome analysis, and community curation. Symposium attendees are invited to participate in these additional events. VENUE: The symposium will take place at the historic Muehlebach Hotel (operated by Marriott) in downtown Kansas City. KANSAS CITY JAZZ AND BARBEQUE: Participants are encouraged to stay Saturday night for an optional evening of jazz and KC barbeque in the historic 18th and Vine district. REGISTRATION: The registration fee will be $275 ($150 for graduate and undergraduate students), and will include a welcome reception Thursday evening, breakfast and lunch on Friday and Saturday, and light refreshments at the Friday poster session. Additional fees apply for the Apollo Workshop Thursday evening and Saturday night dinner. INFORMATION: Contact Doris Merrill at dmerrill@k-state.edu or 785-532-3482. To receive future Symposium announcements, send your contact information to dmerrill@k-state.edu. SYMPOSIUM WEBSITE: www.k-state.edu/agc/symposium.shtml DEADLINES: February 29, 2008 - Poster abstracts (limited to first 150 received) February 29, 2008 - Early registration March 20, 2008 or until room block is filled - Hotel reservations Please share this announcement with colleagues and students! Susan J. Brown, Professor Director, Center for Genomic Studies on Arthropods Affecting Human, Animal and Plant Health by Doris Merrill, Program Coordinator K-State Arthropod Genomics Center Division of Biology, Kansas State University 116 Ackert Hall, Manhattan, KS 66506-4901 (785) 532-3482, dmerrill@k-state.edu www.k-state.edu/agc From heterochromatic from gmail.com Tue Feb 19 14:16:19 2008 From: heterochromatic from gmail.com (sandra schulze) Date: Tue Feb 19 15:45:16 2008 Subject: [Drosophila] picospritzer Message-ID: <5b26f17e0802191116ua82e14as48c5257546d76512@mail.gmail.com> Hello all in Fly land... I am writing a budget for a grant (bone crushing futility etc but make believe is fun) and need a picospritzer. Or some facsimile thereof. Ie., something that has been tried and tested for the delivery of small quantities of DNA into the butt ends of fly embryos. So in the course of looking for the company that makes the picospritzer (technically the Picospritzer III) I can only find a joint operating from the UK. Seems to me there should be a branch/company/etc more local? i.,e mainland US? Bottom line is i need a quote from a company, so here is my question: -does anyone know how to contact said company that makes these things? Please give me the email/address/contact? -does anyone have an alternative? I will welcome advice from those among you with very strong arms using syringes, and i will surely try, but would prefer a machine like a picospritzer - any suggestions? Together with company names/contacts etc. Grant is due 25 Feb. Many thanks, Sandra From Mario.wanischeck from web.de Wed Feb 20 00:45:03 2008 From: Mario.wanischeck from web.de (Mario Wanischeck) Date: Wed Feb 20 07:28:12 2008 Subject: [Drosophila] Re: picospritzer aka picopump In-Reply-To: References: Message-ID: <47bbbdf4$1@news.uni-ulm.de> Hi Sandra! I never tested them for DNA-injection, but the devices from WPI work well in our lab. Try a web-search for "picopump" with your favorite search engine. E.G. Google?s first hit leads to the WPI homepage. I hope this helps, Mario From kim from kimvdlinde.com Wed Feb 20 18:08:54 2008 From: kim from kimvdlinde.com (Kim van der Linde) Date: Wed Feb 20 18:37:57 2008 Subject: [Drosophila] San Diego roommate? Message-ID: <47BCB306.70408@kimvdlinde.com> Hi, Does anybody want to share a room at the Drosophila meeting at San Diego? If so, please contact meat kim@kimvdlinde.com Thanks! kim -- http://www.kimvdlinde.com From raquel.oliveira from bioch.ox.ac.uk Wed Feb 20 13:30:01 2008 From: raquel.oliveira from bioch.ox.ac.uk (Raquel Oliveira) Date: Wed Feb 20 18:38:17 2008 Subject: [Drosophila] GFP-primers Message-ID: <004301c873ee$9acfa7d0$10484381@bioch.ox.ac.uk> Dear fly community, Does any one know any pair of GFP primers that work nicely for fly genotyping by PCR (single fly genomic quick prep)? I have already tried several combinations that amplify nicely from plasmids but not from genomic DNA. :-( If anyone could give me those magic sequences I would be very grateful. Cheers, Raquel Raquel A. Oliveira, PhD Department of Biochemistry University of Oxford South Parks Road Oxford, OX1 3QU UK Tel: +44 1865 275 272 Fax: +44 1865 275 259 raquel.oliveira@bioch.ox.ac.uk From IMcDanie from ScrippsCollege.edu Mon Feb 25 01:11:09 2008 From: IMcDanie from ScrippsCollege.edu (Ivy McDaniel) Date: Mon Feb 25 09:12:50 2008 Subject: [Drosophila] Protein Extract from Larvae Message-ID: <47C1EB7F.5D69.009D.0@ScrippsCollege.edu> Hello Drosophila Community, I'm looking for a good protocol, or even just general tips for making protein extracts from third-instar larvae and adult flies. I'm dealing with a very large protein, and I'm having a lot of trouble avoiding degradation. Grinding adults/larvae/salivary glands directly in boiling SDS loading buffer hasn't worked, nor has one protocol where I included protease inhibitors at every step. Any advice at all would be greatly appreciated! Thanks, Ivy From andre_faustino from hotmail.com Tue Feb 26 07:23:49 2008 From: andre_faustino from hotmail.com (Andre Faustino) Date: Tue Feb 26 12:58:44 2008 Subject: [Drosophila] high/low calorie fly food Message-ID: Dear all, I am trying to conduct some tests and will need a recipe for high and low calorie fly food. Can anyone provide me with these recipes (or let me know were I can find them)? Thank you so much for your help. Best regards, Andr? _________________________________________________________________ Need to know the score, the latest news, or you need your Hotmail?-get your "fix". http://www.msnmobilefix.com/Default.aspx