[Drosophila] GFP-primers

[Raquel Oliveira]

Dear fly community,

 

Does any one know any pair of GFP primers that work nicely for fly
genotyping by PCR (single fly genomic quick prep)? I have already tried
several combinations that amplify nicely from plasmids but not from genomic
DNA. :-(  

 

If anyone could give me those magic sequences I would be very grateful.

 

Cheers,

Raquel 

 

 

 

 

Raquel A. Oliveira, PhD

Department of Biochemistry
University of Oxford
South Parks Road
Oxford, OX1 3QU
UK
Tel: +44 1865 275 272
Fax: +44 1865 275 259

raquel.oliveira from bioch.ox.ac.uk