From sushama from ncbs.res.in Mon Jul 7 00:49:25 2008 From: sushama from ncbs.res.in (sushama@ncbs.res.in) Date: Mon Jul 7 08:31:30 2008 Subject: [Drosophila] intervein Gal4? Message-ID: <36510.192.168.1.1.1215409765.squirrel@mail.ncbs.res.in> Dear All, I am looking for a Gal4 driver that is active in the intervein region of fly wings throughout pupal development. Would be grateful for suggestions. Regards, Sushama From harveje from kellyservices.co.uk Wed Jul 9 04:24:32 2008 From: harveje from kellyservices.co.uk (James Harvey) Date: Wed Jul 9 07:06:34 2008 Subject: [Drosophila] New posting Message-ID: Hello I have a position to advertise, details as below: _____________________________________________________________________________________________________________________________ Syngenta / Kelly Scientific Resources Research Scientist (Two-Year Position) Understanding the mode of action and resistance risk associated with novel insecticidal compounds is a key step in the development of new crop-protection products. This position involves both laboratory-based and computational studies within a team which use genetic, physiological and biochemical approaches to answer critical questions about the way small molecules act on invertebrate pests. The work will involve the handling of chemicals, model organisms and pest insects. Full training will be given on all aspects of safe procedure. The job will involve working both independently and as part of a team and using organisational skills to work flexibly across a number of projects simultaneously. Projects will involve the application of genetic approaches in model or pest organisms to the study of pathophysiological response to insecticide treatment or to the understanding of the basis of insecticide resistance. The job holder will work within a team and provide bioinformatics and molecular biology support for the team?s laboratory-based research program. This may involve cloning work, construction of cDNA libraries, targeted sequencing, or the building of constructs for generation of transgenic invertebrates or for in-vitro expression. Computer based research will involve using bioinformatics tools to query genomic databases, identify gene homologs, construct sequence alignments, and develop strategies for mapping, cloning or building of constructs for transformation work. In addition the job holder may be required to assist with the design and running of behavioural or electrophysiological assays aimed at determining or monitoring mode of action or establishing the intrinsic potencies of new lead chemicals. The job holder will be able to deliver high quality data to tight deadlines, to rapidly learn new tasks and have a creative and scientific approach to problem solving. The position will be filled by someone with postgraduate qualifications and/or practical experience in biological or biochemical research. They will have practical experience of molecular biology techniques and model organism genetics. They will be computer literate with experience of using bioinformatics and database tools. Laboratory experience in any of the following areas would also be of value but is not essential; microinjection, microscopy (esp. confocal or live-tissue calcium imaging), cell or tissue culture, electrophysiology, Drosophila or C.elegans behavioural assays. Good organisation skills and attention to detail are required. He or she will have excellent written and oral communication skills. This role will be based at Syngenta, Jealott's Hill International Research Centre, Bracknell, Berkshire. For further information on this position please contact Jim Goodchild (jim.goodchild@syngenta.com) 01344 416 451 To apply for this position please send a CV and covering letter to Kelly Scientific Resources (ksr_london@kellyservices.co.uk) 020 7278 9809, Job ref. KS1 Closing date for applications 22nd July 2008. _____________________________________________________________________________________________________________________________ Many thanks ! James Harvey Consultant Kelly Scientific Resources 1st Floor, New Premier House 150 Southampton Row London WC1B 5AL Tel 020 7278 9809 Fax 020 7278 9867 harveje@kellyservices.co.uk www.kellyscientific.co.uk Kelly Services... we see work from a People perspective We are currently recruiting for experienced Consultants and Managers to work across our branch network, if you know of someone who you feel would be suited to our business, contact our Internal Recruitment Team on 0870 609 1698. Find out more at www.kellyservices.com About Kelly Services Kelly Services, Inc. (NASDAQ: KELYA, KELYB) is a Fortune 500 company headquartered in Troy, Mich., offering staffing solutions that include temporary staffing services, outsourcing, vendor on-site and full-time placement. Kelly operates in 32 countries and territories. Kelly provides employment to more than 750,000 employees annually, with skills including office services, accounting, engineering, information technology, law, science, marketing, creative services, light industrial, education, and health care. Revenue in 2006 was $5.6 billion. CONFIDENTIAL The information contained in this email and any attachment is confidential. It is intended only for the named addressee(s). If you are not the named addressee(s) please notify the sender immediately and do not disclose, copy or distribute the contents to any other person other than the intended addressee(s) Kelly Services (UK) Ltd. Registered Office: Apple Market House 17 Union Street Kingston upon Thames Surrey KT1 1RR Registered in England and Wales No: 2749906 Please consider the environment before printing From amm from stowers-institute.org Wed Jul 9 13:26:02 2008 From: amm from stowers-institute.org (amst) Date: Wed Jul 9 13:31:11 2008 Subject: [Drosophila] Drosophila eye pseudopupil analysis Message-ID: <18367824.post@talk.nabble.com> Hi, Can any one provide some nice protocol for the pseudopupil analysis to check the adult fly eye. Can one image these structures using confocal or a regular light microscope? Thanks Amit -- View this message in context: http://www.nabble.com/Drosophila-eye-pseudopupil-analysis-tp18367824p18367824.html Sent from the Bio.net - Dros mailing list archive at Nabble.com. From sandra.mueller from embl.de Thu Jul 10 07:33:42 2008 From: sandra.mueller from embl.de (Sandra Mueller) Date: Thu Jul 10 12:18:25 2008 Subject: [Drosophila] FlyC31 injections Message-ID: Dear all, has anyone of you experience with injections into the combined integrase lines with site specific landing site from J. Bischof? I tested 3 lines, but after injection I got only very view larvae out. I'm injecting now since 2 years into W1118 and it still works very good, but with this lines I really have trouble. I tried different DNA concentrations, injection buffer, water, spacing, oil, but nothing helped to increase the survival rate. Can someone help me and share his/her experience with me? My best Sandra From mshalfon from buffalo.edu Mon Jul 14 11:21:41 2008 From: mshalfon from buffalo.edu (Marc S. Halfon) Date: Mon Jul 14 12:33:54 2008 Subject: [Drosophila] looking for a reliable incubator Message-ID: <55A3D6AC-6E90-44D1-AB2A-61F27E476503@buffalo.edu> Hi all- We've been having a lot of problems recently with our fly incubators going down due to corrosion of the coils. Presumably this is due to acids leaching from the food media or something along those lines. We use a pretty standard food recipe. Does anyone have recommendations for fly incubators that hold up well for more than 2-4 years without need replacement of the coils? Thanks, Marc ----------------------------------- Marc S. Halfon, Ph.D. Assistant Professor Dept. of Biochemistry Dept. of Biological Sciences SUNY at Buffalo NY State Center of Excellence in Bioinformatics & Life Science Adjunct Assistant Professor, Molecular and Cellular Biology Roswell Park Cancer Institute B3-307 COEBLS ph: (716) 829-3126 fax: (716) 849-6655 mshalfon@buffalo.edu URL: http://www.ccr.buffalo.edu/halfon mailing address: SUNY at Buffalo-COEBLS 701 Ellicott St. Buffalo, NY 14203 From parkern6 from gmail.com Tue Jul 15 18:38:25 2008 From: parkern6 from gmail.com (Nathan Parker) Date: Wed Jul 16 07:54:36 2008 Subject: [Drosophila] 4 winged fly genotype Message-ID: <547d25100807151638r78308755r99b78be180746c47@mail.gmail.com> Does anyone know the genotypes needed to generate progeny with 2 pairs of wings (Ultrabithorax homeotic transformation). Or even better, does anyone know if this transformation causes any changes in developmental time (specifically L3 to pupariation). Thanks Nathan Parker Dept. of Zoology Michigan State University From scottatg from aol.com Tue Jul 15 15:27:30 2008 From: scottatg from aol.com (scottatg@aol.com) Date: Wed Jul 16 12:48:55 2008 Subject: [Drosophila] Re: Dros Digest, Vol 39, Issue 5 In-Reply-To: <200807151707.m6FH7gO26821@net.bio.net> References: <200807151707.m6FH7gO26821@net.bio.net> Message-ID: <8CAB4D05720C508-21C-A55@mblk-d18.sysops.aol.com> percival scientific, www.percival-scientific.com It is mandatory to have phenolic coated coils with dros. research, otherwise you will have corrosion of the coils. ?Ask me about Miele Glassware Washers. Best Regards, Scott Saveleski Atlantic Technology Group, Inc. 1906 North Hamilton Street, Suite G Richmond, Va. 23230 1-800-359-5153 x2 410-371-8740 cell -----Original Message----- From: dros-request@oat.bio.indiana.edu To: dros@magpie.bio.indiana.edu Sent: Tue, 15 Jul 2008 1:07 pm Subject: Dros Digest, Vol 39, Issue 5 Send Dros mailing list submissions to dros@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/dros or, via email, send a message with subject or body 'help' to dros-request@net.bio.net You can reach the person managing the list at dros-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Dros digest..." Today's Topics: 1. looking for a reliable incubator (Marc S. Halfon) ---------------------------------------------------------------------- Message: 1 Date: Mon, 14 Jul 2008 12:21:41 -0400 From: "Marc S. Halfon" Subject: [Drosophila] looking for a reliable incubator To: dros@magpie.bio.indiana.edu Message-ID: <55A3D6AC-6E90-44D1-AB2A-61F27E476503@buffalo.edu> Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes Hi all- We've been having a lot of problems recently with our fly incubators going down due to corrosion of the coils. Presumably this is due to acids leaching from the food media or something along those lines. We use a pretty standard food recipe. Does anyone have recommendations for fly incubators that hold up well for more than 2-4 years without need replacement of the coils? Thanks, Marc ----------------------------------- Marc S. Halfon, Ph.D. Assistant Professor Dept. of Biochemistry Dept. of Biological Sciences SUNY at Buffalo NY State Center of Excellence in Bioinformatics & Life Science Adjunct Assistant Professor, Molecular and Cellular Biology Roswell Park Cancer Institute B3-307 COEBLS ph: (716) 829-3126 fax: (716) 849-6655 mshalfon@buffalo.edu URL: http://www.ccr.buffalo.edu/halfon mailing address: SUNY at Buffalo-COEBLS 701 Ellicott St. Buffalo, NY 14203 ------------------------------ _______________________________________________ Dros mailing list Dros@net.bio.net http://www.bio.net/biomail/listinfo/dros End of Dros Digest, Vol 39, Issue 5 *********************************** From dmerrill from k-state.edu Thu Jul 17 11:18:26 2008 From: dmerrill from k-state.edu (Doris Merrill) Date: Thu Jul 17 11:25:24 2008 Subject: [Drosophila] Arthropod Genomics Symposium, 6/11-14/09 in Kansas City Message-ID: <005801c8e828$be640160$78738281@ECOGENOFFICE> Save the dates of June 11 to 14, 2009, and plan to attend the.. 3rd ANNUAL ARTHROPOD GENOMICS SYMPOSIUM As more arthropod genomes are sequenced, we are faced with the ever-growing need for databases and bioinformatics tools based on common platforms to support comparative genomics. The Arthropod Genomics Consortium was formed to address these issues. The first projects focus on databases and tools for literature annotation. You are invited to join the fun as we share our progress with the arthropod research community, by providing feedback on these projects and input new possibilities. DATE: June 11 to 14, 2009, in Kansas City at the Marriott Downtown SPONSOR: K-State Arthropod Genomics Center, Kansas State University TENTATIVE SCHEDULE: Thursday evening, June 11 - Keynote presentation and welcome reception Friday & Saturday, June 12 and 13 - Platform and Poster sessions Sunday am, June 14 - Roundtable discussion with the ArthropodBase Consortium. Noon, Sunday, June 14 - Activities will conclude. Speakers who are experts in arthropod genomics and bioinformatics with applications in genomics will be announced soon! Additional speakers will be selected from contributed posters. Demonstrations: Database and bioinformatics tools developers will be available at the meeting to provide hands-on demonstrations. Visit our website, www.k-state.edu/agc, for updates as details are finalized. Share this announcement with colleagues and students! Add your name to the Symposium mailing list, by sending your contact information to dmerrill@k-state.edu. Doris Merrill, Program Coordinator K-State Arthropod Genomics Center Division of Biology, Kansas State University 116 Ackert Hall, Manhattan, KS 66506-4901 (785) 532-3482, dmerrill@k-state.edu www.k-state.edu/agc We apologize if you receive this message multiple times due to cross-listings! From heterochromatic from gmail.com Mon Jul 21 14:57:18 2008 From: heterochromatic from gmail.com (sandra schulze) Date: Mon Jul 21 16:43:37 2008 Subject: [Drosophila] green, blue ANY color TM3's? Message-ID: <5b26f17e0807211257u191fa345jd06db5809b784d46@mail.gmail.com> Hello people of fly land. I am looking for a nice selectable TM3. I mean something that selects homozygous NON balanced larvae. So a GFP balancer or a y+ balancer for the third. Keep pulling up all these lovely descriptions on flybase but 0 stocks. I'll take TM3-GFP or TM3-y+, and if anyone has a confirmed embryonic TM3-GFP that is distinguishable from autofl. I would love that too. Many thank yous, Sandra From marcolnh from hotmail.com Fri Jul 25 08:31:04 2008 From: marcolnh from hotmail.com (Marco Antunes) Date: Fri Jul 25 12:21:05 2008 Subject: [Drosophila] Membrane marker Message-ID: Dear all I'm looking for a transgenic line that expresses any fluorescent protein (GFP, cherry or other) both in the Apical but also Basolateral membrane of Drosophila's embryonic epithelium so that I can visualize clearly all cell contours (all membrane). Do you have any suggestion? Thanks in advance _________________________________________________________________ Confira v?deos com not?cias do NY Times, gols direto do Lance, videocassetadas e muito mais no MSN Video! http://video.msn.com/?mkt=pt-br From r-allada from northwestern.edu Mon Jul 28 12:05:18 2008 From: r-allada from northwestern.edu (Ravi Allada) Date: Mon Jul 28 12:26:00 2008 Subject: [Drosophila] Postdoctoral Position available In-Reply-To: <200807261706.m6QH6TO05776@net.bio.net> References: <200807261706.m6QH6TO05776@net.bio.net> Message-ID: Electrophysiology Postdoctoral Position Available Northwestern University The Allada laboratory is seeking highly motivated individuals with prior experience with electrophysiology for a postdoctoral position. This position is available immediately. Our research is focused on the regulation of circadian and sleep behavior using the fruit fly Drosophila and incorporates patch clamp electrophysiology along with a variety of other approaches including biochemistry, molecular biology, genetics, cell culture, anatomy, and behavior (see Lear et al., Neuron, 2005, Pitman et al., Nature, 2006). This position offers the opportunity to integrate electrophysiological, with molecular genetic and behavioral approaches. See also http://www.northwestern.edu/neurobiology/faculty/allada.html Previous published experience using electrophysiology is essential. Interested individuals should send a brief statement of research interests, CV and the names and contacts for three references by e-mail to r-allada@northwestern.edu or mail to: Ravi Allada, Department of Neurobiology and Physiology, Northwestern University, 2205 Tech Dr., #2-160, Evanston, IL 60208, Tel.: (847) 491-2809, Fax.: (847)491-5211 -- >Ravi Allada >Associate Director, Center for Sleep and Circadian Biology >Associate Professor, Department of Neurobiology and Physiology >Northwestern University >2205 Tech Drive, #2-160 >Evanston, IL 60208 >Office: (847) 491-2809 >Lab: (847) 467-7785 >Fax: (847) 491-5211