From waytz from uchicago.edu Wed Jun 4 09:11:06 2008 From: waytz from uchicago.edu (Adam Waytz) Date: Wed Jun 4 10:21:46 2008 Subject: [Drosophila] Survey on Animals, Nature, Technology for $20 gift to Barnes & Noble Message-ID: <52C374FA-1AA8-471B-9268-EC6065611A61@uchicago.edu> Hello, I am a psychology student at University of Chicago conducting a survey on animals, technology, and nature. It takes only 5-10 minutes to complete and every 20th participant will be emailed a $20 gift certificate to Barnes & Noble. This should be an interesting opportunity to learn things about yourself as well. To participate, click on the link below. You may only take the survey once and will be ineligible if you do so more than once. To begin, click here: http://chicagogsb.qualtrics.com/SE? SID=SV_8wv7XbQvvLw1cJm&SVID=Prod From heterochromatic from gmail.com Fri Jun 6 17:46:48 2008 From: heterochromatic from gmail.com (sandra schulze) Date: Sat Jun 7 08:16:53 2008 Subject: [Drosophila] halocarbon oil Message-ID: <5b26f17e0806061546t4aa74e12s201acaa3c5f3208d@mail.gmail.com> Hello all, I just got a quote for Halocarbon oil series 700 (HC-700) which I am used to using for covering injected embryos, and this oil appears to have gone up dramatically like all the other kinds of...oil. Also the company wants to sell me 2 kg (for near $600) of it which seems a bit excessive. Any alternatives? Oil, of course, but cheaper reliable suppliers? Many thank yous, Sandra From agould from nimr.mrc.ac.uk Thu Jun 12 06:50:24 2008 From: agould from nimr.mrc.ac.uk (Alex Gould) Date: Thu Jun 12 08:06:42 2008 Subject: [Drosophila] Postdoc position in London Message-ID: Role of nutrient sensing in Drosophila growth and development The successful applicant for this 3 year post doctoral position will use genetics, physiology, molecular biology and biochemistry to study the roles of nutrient sensing and metabolism during larval growth and fasting in Drosophila (E. Gutierrez et al. 2007. Nature: 445, 275-280). You should have a Ph.D with a background in developmental biology and previous experience with Drosophila genetics is desirable, although not essential. Informal enquiries can be made to: Dr Alex Gould email: agould@nimr.mrc.ac.uk lab web page: http://www.nimr.mrc.ac.uk/mamdev/gould/ Salary is from ?26,808 to ?32,488 per annum inclusive of Location Allowance. MRC final salary Pension Scheme is available. Applications for this position (Ref: NIMR08/320), including a CV & cover letter must be made online at http://jobs.mrc.ac.uk. If you do not have internet access or experience technical difficulties, please call 01793 301157. The closing date is 10 July 2008. Situated in Mill Hill, London, UK, The National Institute for Medical Research is the largest MRC institute, supporting some 70 research groups and 500 bench scientists. The Institute provides excellent training for researchers in a multi-disciplinary environment and is equipped with state of the art facilities. http://www.nimr.mrc.ac.uk -- ******************************************* Alex P. Gould, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA UK Phone:+44 (0)20 8816 2103 FAX:+44 (0)20 8816 2109 Eileen McCarthy, Secretary:+44 (0)20 8816 2102 http://www.nimr.mrc.ac.uk/mamdev/gould/ From heterochromatic from gmail.com Thu Jun 12 18:15:13 2008 From: heterochromatic from gmail.com (sandra schulze) Date: Thu Jun 12 19:12:01 2008 Subject: [Drosophila] picospritzer II Message-ID: <5b26f17e0806121615r69f62e4bi51b232dbedf06824@mail.gmail.com> Hello all again... Guess what I am doing. I have acquired a Picospritzer II (General Valve corporation) in pieces, and not being of a mechanical bent (not to mention my chromosomal constitution) I would LOVE to read a manual. Anyone out there got a pdf for this thing? I've emailed the company (they are out there under Parker Hannifin Corporation Pneutronics Division) but I need the manual pretty quick so I am trying my luck here as well. Hope you folks in the midwest are coping. What a mess. Mother nature seems rather ticked off these days. Sandra Schulze Western Washington University Bellingham WA From ivymcdaniel from gmail.com Fri Jun 13 09:19:20 2008 From: ivymcdaniel from gmail.com (Ivy McDaniel) Date: Fri Jun 13 10:55:34 2008 Subject: [Drosophila] Working with Fat Bodies Message-ID: Hello all, I am looking for tips for working with fat bodies. I've noticed that although I am processing everything the same way, I see very variable results with RNA extractions from fat bodies, in terms of yield especially. Occasionally I will get preps where I get no RNA. I am confident that the RNA isolation protocol is fine, and that I don't have RNAse contamination (except for in the sample itself). I do my dissections on ice, flash freeze my tubes immediately, and am doing my dissections as rapidly as possible. Has anyone worked with fat bodies before and had similar problems? I'm thinking that my problem is probably coming from dissections, either nicking the gut or being a little too rough with the fat bodies. Got any good tips for doing quick, clean dissections of fat bodies? Thank you all, Ivy McDaniel From Kristi_Wharton from brown.edu Mon Jun 23 18:59:56 2008 From: Kristi_Wharton from brown.edu (Kristi Wharton) Date: Mon Jun 23 20:43:29 2008 Subject: [Drosophila] pH of S2 cells Message-ID: Greetings, Does anyone know the cytoplasmic pH of S2 cells? Kristi -- Kristi A. Wharton, PhD Associate Professor Department of Molecular Biology, Cell Biology and Biochemistry Brown University Box G-L370 Sidney E Frank Hall for Life Sciences 185 Meeting Street Providence, R.I. 02912 Phone: 401 863 1951 FAX: 401 863 1348