From Robert.Maeda from zoo.unige.ch Thu May 1 02:52:48 2008 From: Robert.Maeda from zoo.unige.ch (Rob Maeda) Date: Thu May 1 07:37:58 2008 Subject: [Drosophila] looking for Mariner transposase lines Message-ID: <9848ec8f99956de5b3113ab4f617e366@zoo.unige.ch> Hello, I am looking for a mariner transposase source that expresses in the germline. Dan Hartl's group made some hsp26:mos1 lines a few years ago, but he no longer has the stocks. Does anybody have these stocks or anything similar? Thanks, Robert Maeda University of Geneva Dept of Zoology and Animal Biology 30 Quai E. Ansermet 1211 Gen?ve 4 CH Robert.Maeda@zoo.unige.ch From cameron.nowell from petermac.org Fri May 2 02:28:14 2008 From: cameron.nowell from petermac.org (Nowell, Cameron) Date: Fri May 2 07:18:49 2008 Subject: [Drosophila] Database for Lab Stocks Message-ID: <6D3E01C097DB5C479B25F4063F6385CB022290D6@PMC-EMAIL.petermac.org.au> Hi Everybody, I am curious to know what other labs use to catalogue the various stocks they have in their labs. I am in the process ofdeveloping a stock database for the lab i am in and wanted to make sure i am not re-inventing the wheel. I have searched around a bit and could not find any commercial solution to do what i want. The database i have built up (in access as that is what all the computers here have on them) contains a range of information such is; original source, stock number, genotype, location in the lab (box number and coordinates). When stocks are entered they are classified by a number of paramaters allowign a user to recall stocks in great detail. For example a user could serach for all GAL4 stocks on the second chromosome, or get really complicated and search for say all stocks that have a GFP on the second chromosome, a FRT on the 3rd and a LacZ on the X. Also it is possible to search just by gene name as well. Cheers Cam Cameron Nowell Lab Manager Cell Cycle and Development Peter MacCallum Cancer Centre 7 St Andrews Place East Melbourne, 3002 Victoria AUSTRALIA Phone: +61396563759 Fax: +61396561411 Mobile: +61422882700 This email (including any attachments) may contain confidential and/or legally privileged information and is intended only to be read or used by the addressee. If you are not the intended addressee, any use, distribution, disclosure or copying of this email is strictly prohibited. Confidentiality and legal privilege attached to this email (including any attachments) are not waived or lost by reason of its mistaken delivery to you. If you have received this email in error, please delete it and notify us immediately by telephone or email. Peter MacCallum Cancer Centre provides no guarantee that this transmission is free of virus or that it has not been intercepted or altered and will not be liable for any delay in its receipt. From mottola from ibdm.univ-mrs.fr Tue May 6 09:46:49 2008 From: mottola from ibdm.univ-mrs.fr (Mottola Giovanna) Date: Tue May 6 12:52:30 2008 Subject: [Drosophila] (no subject) Message-ID: <813cbd220c919cc665360424baa7bbb5@ibdml.univ-mrs.fr> Hi everybody, I am looking for a fly stock having a marker to visualize mitotic clones in adult wings (like forked or multiple hairs) together with FRT40A. Could you help me, please? Cheers Giovanna Mottola Institut de Biologie du D?veloppement de Marseille-Luminy (IBDML) UMR 6216 Case 907, Parc Scientifique de Luminy 13288 Marseille Cedex 9 France Mail: mottola@ibdml.univ-mrs.fr Tel: 33-(0)4 9126 9744 Fax: 33-(0)4 9126 9748 http://www.ibdm.univ-mrs.fr/ From narelle.tunstall from anu.edu.au Wed May 7 00:31:47 2008 From: narelle.tunstall from anu.edu.au (Narelle Tunstall) Date: Wed May 7 08:02:05 2008 Subject: [Drosophila] Position available - Australian Drosophila Facility Manager Message-ID: <48213EC3.30003@anu.edu.au> Job Posting: Australian Drosophila Biomedical Research Facility Manager Australian National University Postdoctoral Fellow – Academic Level A Reference: RSBS 4810 The Molecular Genetics and Evolution Group at the Research School of Biological Sciences wishes to appoint a Manager to oversee the Australian Drosophila Biomedical Research Support Facility (OzDros), an NH&MRC Enabling Facility that supports Drosophila biomedical research throughout Australia. The facility houses many thousands of Drosophila stocks, and supplies them to Australian laboratories. It also carries out stock importation in accordance with AQIS regulations and is in the process of establishing Drosophila transformation technologies. The Manager is expected to have a PhD in genetics or a combination of a B.Sc. or equivalent degree that included genetics and extensive experience in laboratory management. The manager is responsible for the maintenance of Drosophila stocks and stock records, liaising with participating scientists throughout Australia, receiving, recording and responding to requests for stocks and advising Australian researchers on the use of Drosophila as a model organism. The Manager supervises a small number of technical staff who work in the facility. The successful candidate must have a research-level knowledge of and experience in genetics, must be meticulous in the maintenance of genetically defined laboratory stocks and have outstanding organizational and management skills. Selection Criteria: http://info.anu.edu.au/hr/jobs/ or from Virginia Riddle, HR Manager, RSBS, T: 02 6125 4752, E: Virginia.Riddle@anu.edu.au Enquiries: Professor Robert Saint, T: 02 6125 2383, E: Robert.Saint@anu.edu.au Closing Date: 6 June 2008 -- Narelle Tunstall, PhD Manager Australian Drosophila Biomedical Research Facility www.ozdros.com Ph: +612 6125 1089 Research School of Biological Sciences The Australian National University GPO Box 475 Canberra ACT 2601 From hilde from flymine.org Thu May 8 05:35:06 2008 From: hilde from flymine.org (Hilde Janssens) Date: Thu May 8 07:29:22 2008 Subject: [Drosophila] FlyBase / FlyMine course - 12th June Message-ID: <4822D75A.7020008@flymine.org> Dear researchers, The next joint FlyBase/FlyMine workshop will be held on Thursday June 12th in the Department of Genetics. FlyBase and FlyMine are databases which mainly focus on Drosophila data. This workshop is targeted primarily at new users, though all are welcome. We will start by providing an overview of the data and tools present on each website. The differences between FlyBase and FlyMine, and why you might use one rather than the other for a particular query, will also be discussed. We will then explore how to perform advanced and complex queries using the various tools available on our websites. Teaching will be through a combination of short presentations, live demonstrations and hands-on practice. Course details: - Date: 12th June 2008 - Time: 9.30-12.45 and 1.45-5.30 - Venue: Genetics Room G12 - Code: ET27 - Credits: 2 Please register for this workshop at: http://www.bio.cam.ac.uk/gradschool/current/courses/bioinformatics.html The FlyBase and FlyMine teams. www.flybase.org www.flymine.org From kmbhat from utmb.edu Wed May 7 18:17:00 2008 From: kmbhat from utmb.edu (Krishna Bhat) Date: Thu May 8 07:29:50 2008 Subject: [Drosophila] Fly stock center Message-ID: There is an excellent stock center facility that I helped set up and this center maintains stocks for a price (www.modelbiosystems.com). It is located in Bangalore India and some of us have been keeping our stocks there for now ~2 years. They have done an excellent job in maintaining the stocks in great condition. You can contact them at: malli.varanasi@gmail.com From sa08383 from wotan.mdacc.tmc.edu Wed May 21 10:22:06 2008 From: sa08383 from wotan.mdacc.tmc.edu (Kathleen Gajewski) Date: Wed May 21 11:38:18 2008 Subject: [Drosophila] looking for lysosome stain Message-ID: Hi to all out in FlyLand, I am looking for a suitable stain for lysosomes in FIXED blood cell smears. All the stains I've been referred to so far are for use in living cells. Does anyone know of an appropriate stain & protocol for fixed material?? Many Thanks KG From andre_faustino from hotmail.com Thu May 29 07:24:58 2008 From: andre_faustino from hotmail.com (Andre Faustino) Date: Thu May 29 08:48:43 2008 Subject: [Drosophila] Protocols and methods to homogenize flies In-Reply-To: <6D3E01C097DB5C479B25F4063F6385CB022290D6@PMC-EMAIL.petermac.org.au> References: <6D3E01C097DB5C479B25F4063F6385CB022290D6@PMC-EMAIL.petermac.org.au> Message-ID: Dear all, =20 I am trying to quantify proteins, sugars and lipids in flies but having tro= uble with the homogenization step. So far I am homogenizing flies with a motor pestle in PBS. Then I perform a= series of spins to remove tissue. I use the supernatant to conduct the tests, but I am having troubles in get= ting sufficient amount of material, especially to conduct the tests to eval= uate lipid content of my samples. This leads to problems in sample comparis= on since different levels of homogenization lead to different results obtai= ned. Can someone please provide me with protocols and tips for homogenization of= whole flies? =20 Thanks, =20 Andr=E9 _________________________________________________________________ E-mail for the greater good. Join the i=92m Initiative from Microsoft. http://im.live.com/Messenger/IM/Join/Default.aspx?source=3DEML_WL_ GreaterG= ood=