From gilbertd from net.bio.net Sun Sep 7 10:37:51 2008 From: gilbertd from net.bio.net (Don Gilbert) Date: Mon Sep 8 08:08:15 2008 Subject: [Drosophila] Arthropod gene orthologs at insects.eugenes.org/arthropods/ Message-ID: <200809071537.m87Fbpl25229@net.bio.net> Drosophila genomicists, You may find useful material in this new orthology comparison of Arthropod genes at http://insects.eugenes.org/arthropods/ euGenes/Arthropods contains computed protein orthologs among 13 species: 3 mosquitoes, 3 drosophila, 2 hymenoptera, 3 other insects, a crustacean and an arachnid. These can be searched by gene names, descriptions and IDs. You can also select gene groups by presence or absence in a species. Find genes also by BLAST, including PSI-BLAST, of the ortholog groups. You can for instance find the 7 cases in 27497 groups where the other insects, including Dros. pse. and Dros. moj., have a gene that Dros. mel. is apparently missing (I think I've found most of these in Dmel's genome :) You can also find these Arthropod gene pages via Google searches, Google: daphnia drosophila ixodes gene homeobox Google: drosophila aedes aphid gene ribosomal protein L13a especially if you add these newer genome organisms others do not yet report. Google searches are not as certain to pick up all the arthropod gene groups as the search at euGenes/arthropods. This arthropod gene service is preliminary; your comments and suggestions are welcome. Please also comment if you are interested in having a public discussion list focused on Arthropod genomics. Many of these new genomics studies in insects and relatives have similar needs and themes, where a common discussion list could serve. The Bionet news group (http://www.bio.net/) is an easy option for this; it now hosts several organism-genome groups, including Drosophila, Arabidopsis, Medicago, yeast. -- Don Gilbert From sandra.mueller from embl.de Mon Sep 8 04:05:00 2008 From: sandra.mueller from embl.de (Sandra Mueller) Date: Mon Sep 8 08:08:44 2008 Subject: [Drosophila] pre-pulled injection capillaries Message-ID: Hello to all Drosophila injecting people, All of you may know how essential it is to have a good needle! I'm currently using pre pulled capillaries from Eppendorf (Femtotip I). But they have a big variance in length, diameter and they are not open (as Eppendorf claim in their description) Is anyone of you using the Femtotips? Or what capillaries do you use? It would be great if you can share your experience with me. Maybe there is an other company with better pre pulled capillaries where I can order from Germany? Many thanks, Sandra From priit.joers from uta.fi Wed Sep 10 09:23:30 2008 From: priit.joers from uta.fi (Priit Joers) Date: Wed Sep 10 10:08:34 2008 Subject: [Drosophila] Integration vectors with two promoters? Message-ID: <48C7D862.4050809@uta.fi> Does anybody know if there are Drosophila integration vectors available with two promoters that can be used to clone and express two different proteins from the same construct after single integration event? Thanks in advance, Priit Joers From harry.bergeron from yahoo.com Wed Sep 17 11:13:33 2008 From: harry.bergeron from yahoo.com (som3d00d) Date: Wed Sep 17 11:22:31 2008 Subject: [Drosophila] Integration vectors with two promoters? In-Reply-To: <48C7D862.4050809@uta.fi> References: <48C7D862.4050809@uta.fi> Message-ID: <19535736.post@talk.nabble.com> http://www.pnas.org/content/103/47/17813 will be useful to modify the construct described in the above. sd Priit Joers wrote: > > Does anybody know if there are Drosophila integration vectors available > with two promoters that can be used to clone and express two different > proteins from the same construct after single integration event? > > > Thanks in advance, > > Priit Joers > > _______________________________________________ > Dros mailing list > Dros@net.bio.net > http://www.bio.net/biomail/listinfo/dros > > -- View this message in context: http://www.nabble.com/Integration-vectors-with-two-promoters--tp19415860p19535736.html Sent from the Bio.net - Dros mailing list archive at Nabble.com. From kaedwar from ilstu.edu Sun Sep 21 20:06:36 2008 From: kaedwar from ilstu.edu (Kevin Edwards) Date: Mon Sep 22 06:22:43 2008 Subject: [Drosophila] Tenure Track Position Available Message-ID: Developmental Biology Tenure Track Position Available Illinois State University The job ad is posted here: http://www.cop.ilstu.edu/eo/facVac.taf? _function=detail&facDept_uid1=1&pos=805 -Kevin Edwards From ogrady from nature.berkeley.edu Tue Sep 23 15:25:24 2008 From: ogrady from nature.berkeley.edu (Patrick O'Grady) Date: Tue Sep 23 18:09:51 2008 Subject: [Drosophila] Meeting: Drosophila Taxonomy, Evolution and Genetics Message-ID: The Drosophila Species Stock Center in San Diego will host a special symposium on Drosophila Taxonomy, Evolution and Genetics. This symposium will be held on Sunday 23 November 2008 and will address proposed taxonomic changes in the genus Drosophila and their implications to the fields of genetics, evolution, development and neurobiology. Participants include Michael Ashburner, Gerhard Baechli, Rob DeSalle, Kenneth Kaneshiro, Thom Kaufmann, William McGinnis, and Masanori Toda. Registration for this event is $150 and includes a Saturday night banquet at the Birch Aquarium, buffet lunch on Sunday and a contributed poster session. Details can be found at: https:// stockcenter.ucsd.edu/info/workshopschedule2008.php#Symposium Please contact Dr. Therese Markow (tmarkow@ucsd.edu) or Dr. Patrick O'Grady (ogrady@nature.berkeley.edu) for additional information. -- *************************************** Patrick M. O'Grady, Ph.D. Assistant Professor University of California, Berkeley Department of Environmental Science, Policy & Management Division of Organisms and the Environment 137 Mulford Hall Berkeley, CA 94720 ogrady@nature.berkeley.edu tele: (510) 642-0662 fax: (510) 643-5098 http://nature.berkeley.edu/ogradylab/index.html *************************************** From ogrady from nature.berkeley.edu Tue Sep 23 15:26:13 2008 From: ogrady from nature.berkeley.edu (Patrick O'Grady) Date: Tue Sep 23 18:09:55 2008 Subject: [Drosophila] Workshop: Drosophila Species Identification and Culture Message-ID: The 8th Annual Drosophila Species Workshop will be held at UCSD November 20-23, 2008. For the complete announcement and details see https://stockcenter.ucsd.edu/info/workshopschedule2008.php#Workshop To apply contact: Therese Markow tmarkow@ucsd or Patrick O'Grady ogrady@nature.berkeley.edu -- *************************************** Patrick M. O'Grady, Ph.D. Assistant Professor University of California, Berkeley Department of Environmental Science, Policy & Management Division of Organisms and the Environment 137 Mulford Hall Berkeley, CA 94720 ogrady@nature.berkeley.edu tele: (510) 642-0662 fax: (510) 643-5098 http://nature.berkeley.edu/ogradylab/index.html *************************************** From wangning212 from gmail.com Tue Sep 30 12:56:08 2008 From: wangning212 from gmail.com (wangning212@gmail.com) Date: Tue Sep 30 13:22:05 2008 Subject: [Drosophila] Help for rucuca mapping Message-ID: Hi, there, I'm mapping a mutant at 3nd chromsome with rucuca. However, I was confused between the sr and ca since I can find normal red, scarlet, claret and yellow from progeny. Anybody who know how to distinguish sr, ca and sr/ca? Thanks alot! Ning