[Drosophila] Re: Dros Digest, Vol 50, Issue 7

cris corio via dros%40net.bio.net (by cristiancorio from gmail.com)
Mon Jun 22 08:25:42 EST 2009


Dear Anna, I am using AFA for diferent micoscopy tecnics (In toto
inmuno-histochemistry and resin inclusion for conventional staining) and in
both cases i recomend this fixation mixture where hight preservation
morphology is needed. Besides im not sure about the AFA cross-linking
effects in RNA, but i think is not larger than paraformaldehyde.
I hope to be useful


2009/6/19 <dros-request from oat.bio.indiana.edu>

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>   1. Ovary Cryosections (Anna Hitrik)
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> Message: 1
> Date: Fri, 19 Jun 2009 12:14:04 +0300 (IDT)
> From: Anna Hitrik <anna.hitrik from weizmann.ac.il>
> Subject: [Drosophila] Ovary Cryosections
> To: Dros from magpie.bio.indiana.edu
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> Dear community,
>
> I am trying to perform cryosections of Drosophila ovary. I am doing
> paraformaldehyde fixation before freezing the ovaries in O.C.T. and then cut
> the frozen tissue to 7 micron sections. The aim is to extract RNA from the
> single cells (Laser Capture Microscopy). The problem is that I can not
> obtain the sutisfying morphology of the sections and can not distinguish
> between cells. Does anyone have any suggestions for improving the
> morphology?
>
> Thank you in advance,
>
> Anna Hitrik
>
>
>
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