From joan.sim1 from gmail.com Sat Oct 3 03:54:01 2009 From: joan.sim1 from gmail.com (Joan Sim) Date: Sat Oct 3 07:05:56 2009 Subject: [Drosophila] please help, problems with immunostaining Dros larva brain Message-ID: <25726895.post@talk.nabble.com> Dear all, I am facing problems with homogenous immunostaining of the whole Dosophila larva (3rd instar) brain, as I found after confocal microscopy that the staining / antibody penetration into the interior of the brain was poor. May I know what are some of the solutions to overcome this problem and which holder device should I perform this immunostaining experiment in so as to obtain good mixing during the immunostaining process and do not have to worry about the loss of the very small-sized larvae brains? I would greatly appreciate any suggestions given. Thank you very much for your kind help. Best regards, Joan Sim -- View this message in context: http://www.nabble.com/please-help%2C-problems-with-immunostaining-Dros-larva-brain-tp25726895p25726895.html Sent from the Bio.net - Dros mailing list archive at Nabble.com. From David_Rand from brown.edu Wed Oct 7 13:03:31 2009 From: David_Rand from brown.edu (David Rand) Date: Wed Oct 7 13:22:31 2009 Subject: [Drosophila] Postdoc: Mitochondrial genetics of aging at Brown University Message-ID: <4E8BB058-DBEC-48F9-9B09-455FED6CF566@brown.edu> Postdoc at Brown University: Mitochondrial genetics of aging in Drosophila A postdoc position is available in the laboratory of David Rand and Brown University to work on nuclear-mitochondrial genetic interactions affecting longevity. The general theme of this NIH funded project is to study the epistatic interactions among nuclear and mitochondrial genotypes that diet-dependent alterations in longevity and related, physiological and biochemical traits in Drosophila. Mitochondrial genotypes from different strains and species of the melanogaster subgroup are placed on various nuclear genetic backgrounds to dissect the genetic basis of longevity and the extension of longevity by diet restriction. The project involves demography experiments, molecular and quantitative genetic assays of Drosophila strains, genetic mapping experiments, gene expression and biochemical assays of specific genotypes, and inferences from molecular population genetics and evolution. Applicants must have completed their PhD before starting the position, and have a strong record of accomplishment from their dissertation work. Applicants should have some combination of experience with molecular genetic techniques, Drosophila genetics, longevity of life history analyses, quantitative genetics, biochemical assays, molecular evolution, computational analysis, and an interest in evolution or genetics. The appointment is open for up to 3 years, and successful candidates will receive guidance in the preparation of an NIH NRSA Fellowship to pursue related projects. The position is available now and applications will be reviewed until the position is filled. To apply please send 1) a CV, 2) a short statement of research interests and experience, including a paragraph on how your previous experience makes you well qualified for this position, and 3) contact information for three references to: David Rand email: David_Rand@brown.edu Department of Ecology and Evolutionary Biology Box G-W, 80 Waterman Street Brown University Providence, RI 02912 Voice: (401) 863-2890 (Office) (401) 863-1063 (Lab) Fax: (401) 863-2166 web pages: http://research.brown.edu/research/profile.php?id=1100924991&r=1 http://www.brown.edu/Departments/EEB/rand/index.htm The Department of Ecology and Evolutionary Biology at Brown is an interactive group with strengths that span genomics, evolutionary biology, functional morphology, ecology, and environmental science. There are close ties to the Department of Molecular and Cellular Biology, and the Center for Computational Molecular Biology, as well as the Marine Biological Laboratory in Woods Hole http://www.brown.edu/Departments/EEB/index.html http://www.brown.edu/Departments/Molecular_Biology/ http://www.brown.edu/Research/CCMB/ http://www.mbl.edu http://jbpc.mbl.edu/ http://ecosystems.mbl.edu/ Information on postdoctoral studies at Brown can be found here: http://biomed.brown.edu/grad-postdoc/ Providence is widely recognized as the Venice of New England and is wonderful place to live. http://www.providenceri.com/ ---------------------------------------------------------------------------------------------- David M. Rand Professor of Biology Department of Ecology and Evolutionary Biology Box G-W, 80 Waterman Street Brown University Providence, RI 02912 Voice: (401) 863-2890 (Office - Walter Hall 202) (401) 863-1063 (Lab - BioMed Center 516-518) Fax: (401) 863-2166 email: David_Rand@brown.edu web pages: http://www.brown.edu/Departments/EEB/rand/index.htm http://research.brown.edu/research/profile.php?id=1100924991&r=1 From samarezzat0 from yahoo.com Wed Oct 7 08:57:53 2009 From: samarezzat0 from yahoo.com (Samar Ezzat) Date: Wed Oct 7 13:23:01 2009 Subject: [Drosophila] study of Drosophila behavior Message-ID: <870575.51884.qm@web112008.mail.gq1.yahoo.com> Dear all, ? I want to study the?walking behavior? of some trangenic lines of Drosophila . i will use videocamera , but from where can i get? the photo optical data analyzer? or? the? Frame -by -Frame? film analysis software ? ? ? I will apperciate any suggestions given . ? ? with regards, behavior From bazinetc from stjohns.edu Fri Oct 9 16:33:47 2009 From: bazinetc from stjohns.edu (Chris Bazinet) Date: Fri Oct 9 17:01:10 2009 Subject: [Drosophila] Seeking "Exploded view" diagram of Drosophila Message-ID: <25828219.post@talk.nabble.com> I'm looking for some nice diagrams of the Drosophila adult,ideally one that includes an exploded view showing some major internal organs (e.g. gonads, brain, gut, etc.) CAn anyone point me to a nice one? Thanks Chris Bazinet -- View this message in context: http://www.nabble.com/Seeking-%22Exploded-view%22-diagram-of-Drosophila-tp25828219p25828219.html Sent from the Bio.net - Dros mailing list archive at Nabble.com. From kaufman from indiana.edu Sun Oct 11 12:17:56 2009 From: kaufman from indiana.edu (Thom Kaufman) Date: Sun Oct 11 22:55:53 2009 Subject: [Drosophila] Seeking "Exploded view" diagram of Drosophila In-Reply-To: <25828219.post@talk.nabble.com> References: <25828219.post@talk.nabble.com> Message-ID: <3DA3780A-866D-41FA-87CD-C85E9F99F2ED@indiana.edu> You can find several of these on FlyBase. From the home page go to Image Browse and click on Adult. You will then see a list of available images. If you use any of these we would appreciate your citing their source and FlyBase. --tk kaufman@indiana.edu On Oct 9, 2009, at 5:33 PM, Chris Bazinet wrote: > > I'm looking for some nice diagrams of the Drosophila adult,ideally > one that > includes an exploded view showing some major internal organs (e.g. > gonads, > brain, gut, etc.) CAn anyone point me to a nice one? > > Thanks > > Chris Bazinet > > -- > View this message in context: http://www.nabble.com/Seeking-%22Exploded-view%22-diagram-of-Drosophila-tp25828219p25828219.html > Sent from the Bio.net - Dros mailing list archive at Nabble.com. > > _______________________________________________ > Dros mailing list > Dros@net.bio.net > http://www.bio.net/biomail/listinfo/dros From cje2 from york.ac.uk Mon Oct 12 11:37:57 2009 From: cje2 from york.ac.uk (chris elliott) Date: Mon Oct 12 11:44:40 2009 Subject: [Drosophila] Re: study of Drosophila behavior In-Reply-To: References: Message-ID: Samar Ezzat wrote: > Dear all, > > I want to study the walking behavior of some trangenic lines of Drosophila . i will use videocamera , but from where can i get the photo optical data analyzer or the Frame -by -Frame film analysis software ? > > > I will apperciate any suggestions given . > > > > with regards, > behavior > > > ImageJ is very good, and will allow you to do frame/frame analysis, even removing any constant background chris From matthewk from indiana.edu Mon Oct 12 17:07:11 2009 From: matthewk from indiana.edu (Kathy Matthews) Date: Mon Oct 12 17:09:29 2009 Subject: [Drosophila] Stocks to be removed from the BDSC collection Message-ID: <4AD3A88F.7000901@indiana.edu> Selected obsolete, redundant, or low-use stocks are periodically removed from the Bloomington Drosophila Stock Center collection to make room for new additions. The "pruning page" at http://flystocks.bio.indiana.edu/Cull/pruning.htm provides links to lists of stocks that have been identified for discard and states the last order date for a given set of stocks. Please review these lists for stocks you might want to order before they are discarded. If you think a listed stock warrants continued maintenance at the BDSC please contact us. -- Kathy Matthews Bloomington Drosophila Stock Center Dept. of Biology, Indiana University 1001 E. 3rd St. Bloomington, IN 47405-7005 email: matthewk@indiana.edu voice: 812-855-5782 fax: 812-855-2577 From aribasu from mail.med.upenn.edu Tue Oct 13 14:01:56 2009 From: aribasu from mail.med.upenn.edu (Dr. Arindam Basu) Date: Tue Oct 13 14:14:21 2009 Subject: [Drosophila] SL2 or Kc transfection Message-ID: <680036753.3685941255460516705.JavaMail.root@zm-mbx-levy.zimbra.upenn.edu> Dear All, I am trying to test some putative fly promoter regions using luciferase assays. My transcription factors are in pcDNA3.1. I saw some regulation of the promoters by the factors initially but when I tried to repeat them, its not working. The initial results were quite convincing. Now there seems to be no or wacky effect of the factors on the same promoters. Is there any specific reason which might be the cause for this negative result. Or its impossible to get expression from pCDNA3.1 vectors in fly cell lines. Any input in this matter will be really appreciated. Thank you very much Arindam -- From kafeikatie from yahoo.com Tue Oct 20 04:43:48 2009 From: kafeikatie from yahoo.com (ko) Date: Tue Oct 20 07:11:12 2009 Subject: [Drosophila] location of GAL4 CD8GFP third chromosome insertion Message-ID: <25972373.post@talk.nabble.com> Hi. Does anyone know the insertion site of the P{UAS-mCD8::GFP.L}LL6 p-element found in Bloomington stock # 5130 (y[1] w[*]; Pin[Yt]/CyO; P{w[+mC]=UAS-mCD8::GFP.L}LL6). It was generated by Liqun Luo's lab and found in : Lee, T., Luo, L. (1999). Mosaic analysis with a repressible neurotechnique cell marker for studies of gene function in neuronal morphogenesis. Neuron 22(3): 451--461. Any mapping data would be most welcome. Thanks. Kate. -- View this message in context: http://www.nabble.com/location-of-GAL4-CD8GFP-third-chromosome-insertion-tp25972373p25972373.html Sent from the Bio.net - Dros mailing list archive at Nabble.com. From jaiganesha from gmail.com Tue Oct 20 12:58:04 2009 From: jaiganesha from gmail.com (Gurudatta Baraka) Date: Tue Oct 20 14:16:55 2009 Subject: [Drosophila] Re: Dros Digest, Vol 54, Issue 7 In-Reply-To: <200910201703.n9KH3WM11792@net.bio.net> References: <200910201703.n9KH3WM11792@net.bio.net> Message-ID: <84b2a75a0910201058r34416f39y9a9bf80f783b4369@mail.gmail.com> why it is not avialable from bloomington centre ? On Tue, Oct 20, 2009 at 1:03 PM, wrote: > Send Dros mailing list submissions to > dros@net.bio.net > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/dros > or, via email, send a message with subject or body 'help' to > dros-request@net.bio.net > > You can reach the person managing the list at > dros-owner@net.bio.net > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Dros digest..." > > > Today's Topics: > > 1. location of GAL4 CD8GFP third chromosome insertion (ko) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 20 Oct 2009 02:43:48 -0700 (PDT) > From: ko > Subject: [Drosophila] location of GAL4 CD8GFP third chromosome > insertion > To: dros@magpie.bio.indiana.edu > Message-ID: <25972373.post@talk.nabble.com> > Content-Type: text/plain; charset=us-ascii > > > Hi. Does anyone know the insertion site of the P{UAS-mCD8::GFP.L}LL6 > p-element found in Bloomington stock # 5130 (y[1] w[*]; Pin[Yt]/CyO; > P{w[+mC]=UAS-mCD8::GFP.L}LL6). It was generated by Liqun Luo's lab and > found in : Lee, T., Luo, L. (1999). Mosaic analysis with a repressible > neurotechnique cell marker for studies of gene function in neuronal > morphogenesis. Neuron 22(3): 451--461. Any mapping data would be most > welcome. Thanks. Kate. > -- > View this message in context: > http://www.nabble.com/location-of-GAL4-CD8GFP-third-chromosome-insertion-tp25972373p25972373.html > Sent from the Bio.net - Dros mailing list archive at Nabble.com. > > > > ------------------------------ > > _______________________________________________ > Dros mailing list > Dros@net.bio.net > http://www.bio.net/biomail/listinfo/dros > > End of Dros Digest, Vol 54, Issue 7 > *********************************** > -- Dr Gurudatta B.V., Ph.D. Post Doctoral Fellow, 1071B, Rollins Research Centre Biology Department, MS:1940-001-1AC Emory university, Atlanta, GA, USA 30322 phone Lab:+1 404-727-4250 Cell Phone:+1 678-643-6243 jaiganesha@gmail.com From gvogler from burnham.org Thu Oct 22 18:50:06 2009 From: gvogler from burnham.org (Georg Vogler) Date: Fri Oct 23 08:01:09 2009 Subject: [Drosophila] problems with paper lids for stock bottles Message-ID: <009201ca5372$61c380a0$254a81e0$@org> Hi! We've been using paper lids from Genesee Scientific for our stock bottles (6 oz. square bottles). But now Genesee has changed these paper lids (or their supplier) and those are not great at all (they do not sit tight, bend easily and the little lever to lift the lid tears is easily tearing). Does anyone know of other suppliers for paper lids (42.75mm in diameter) that are reliable? Any help or suggestion on this topic would be greatly appreciated! Best, Geo From rkraft from email.arizona.edu Mon Oct 26 12:44:47 2009 From: rkraft from email.arizona.edu (Robert Kraft) Date: Mon Oct 26 14:56:27 2009 Subject: [Drosophila] searching for EP stock Message-ID: <20091026104447.1oo2kgg84sgc8scs@www.email.arizona.edu> Now that the Szeged Centre has closed, does anyone know whether the Rorth EP collection is intact anywhere else? I am looking for EP(3)0809, does anyone happen to have this stock? From kercook from indiana.edu Mon Oct 26 15:04:36 2009 From: kercook from indiana.edu (Kevin Cook) Date: Mon Oct 26 15:05:14 2009 Subject: [Drosophila] searching for EP stock Message-ID: <4AE600D4.2000806@indiana.edu> Check with the Kyoto Stock Center. They were taking a bunch of the EP insertions. Kevin -- Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology Indiana University 1001 E. Third St. Bloomington, IN 47405-7005 kercook@indiana.edu 812-856-1213 (office), 812-855-2577 (fax) http://flystocks.bio.indiana.edu