[Drosophila] Protein biotinylation assay using Drosophila brain

Runa Hamid via dros%40net.bio.net (by runahamid from googlemail.com)
Thu Aug 20 03:52:53 EST 2015


Dear Drosophilist's,

I wish to do protein biotinylation assay for a membrane protein using
Drosophila
brain. I know its easy to do using cell lines. Is it possible if
I trypsinize the larval brain and disperse the cells and directly do the
assay after giving washes?

Any suggestions on this idea?

Awaiting response!

Regards,
Runa


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