From luojun237 from gmail.com Thu Oct 16 07:58:21 2008 From: luojun237 from gmail.com (Jun Luo) Date: Thu Oct 16 08:11:50 2008 Subject: [Drosophila] Request the attB-P(acman)-ApR plasmid Message-ID: <200810162058079599917@gmail.com> Hi all, Does anyone has the attB-P(acman) plasmid? We have trouble to order it from DGRC because of the inspection of Chinese Customhouse. If the plasmid is dropped in filter paper and mailed by regular mail in an envelop, the customhouse will not check it. I would really appreciate if anyone can send this plasmid to me by that way. Best regards, Jun Luo Model Animal Research Center Nanjing University 12 Xue-fu Road, Pu-kou district Nanjing, P.R. China 210061 Tel: 86-25-58641509 From guichet from ijm.jussieu.fr Wed Oct 22 11:37:37 2008 From: guichet from ijm.jussieu.fr (Antoine Guichet) Date: Wed Oct 22 12:07:36 2008 Subject: [Drosophila] Injectmatic tas Message-ID: Dear all, does anyone has any experience with the Sleeper TAS system from the Geneva based company INJECTMATIC or with longterm effects of nitric oxide exposure of Drosophila? The TAS device extracts and concentrates nitrogen from the air which is then used to anesthetize Drosophila. For more details see http://www.injectmatic.com/produits.php?lang=fr&idProduit=16 I thank you very much for your help Antoine Guichet -- **************************************** Dr. Antoine Guichet Equipe Polarit? et Morphogen?se Institut Jacques Monod CNRS, Universit?s Paris 6 et 7 2, place Jussieu 75251 Paris Cedex 05 France tel + 33 1 44 27 40 94 fax +33 1 44 27 52 65 e-mail guichet@ijm.jussieu.fr **************************************** From kcook from bio.indiana.edu Wed Oct 22 12:35:34 2008 From: kcook from bio.indiana.edu (Kevin Cook) Date: Wed Oct 22 12:36:07 2008 Subject: [Drosophila] Injectmatic tas Message-ID: <200810221735.m9MHZVJW013603@mail-relay.iu.edu> Antoine-- I purchased an Injectamatic Sleeper TAS a few years ago from Genesee Scientific. It works exactly as described. My impression is that flies wake up slightly more slowly than when they are anesthetized with carbon dioxide (though much more quickly than ether anesthesia), but I never found it to be a problem. I never had any reason to think that nitric oxide was any more deleterious than carbon dioxide, but I did not do any kind of careful study. My only complaint about using the Sleeper unit is that the directions indicate that it must be used intermittently for no more than one minute at a time. I'm not quite sure why this is, or exactly how the inventors expected users to apply this rule. The Sleeper comes with a foot pedal to control a pump in the unit. I simply pressed the foot pedal for awhile, stopped for a bit, pressed it some more, etc. In this way, I could keep flies asleep for several minutes. I don't think I hurt the gas generator doing this (though I burned out a solenoid valve that I had to replace for ~$30). It took awhile to get used to pressing the pedal rhythmically. Maybe the inventors could provide some guidance on this issue. I never asked and maybe I was doing things wrong...... The pump makes it more noisy than using CO2 and your labmates might not like listening to it, but it's a reasonable alternative. Kevin __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@bio.indiana.edu From heterochromatic from gmail.com Thu Oct 23 14:42:18 2008 From: heterochromatic from gmail.com (sandra schulze) Date: Thu Oct 23 16:31:11 2008 Subject: [Drosophila] fusion cassettes Message-ID: <5b26f17e0810231242u5a4469bal52e7db8399403392@mail.gmail.com> Hello people in flyland... Does anyone out there have a pCaSpeR-hs-ACT vector with a GFP cassette into which i can clone things in frame to make GFP-fusion proteins (N or C term)? Ideally I am also very interested in finding a similar set up with FLAG, HA or myc. I got excited with the pelicans and the stingers but they don't seem to allow for fusions, rather they look like reporters for putative regulatory elements. Please contact me if you can point me in the right direction. Many thanks, Sandra From lcourtneysmith from gmail.com Tue Oct 28 13:23:14 2008 From: lcourtneysmith from gmail.com (L. Courtney Smith) Date: Tue Oct 28 13:41:27 2008 Subject: [Drosophila] Re: tenture track faculty position Message-ID: <660183310810281123k57002400ue3a4e27b6550479e@mail.gmail.com> Please post the advertisement. Thanks. ________________________________________________ TENURE TRACK FACULTY POSITION in MOLECULAR BIOLOGY The Department of Biological Sciences invites applications for a tenure track Assistant Professor for September, 2009. We are seeking a broadly trained scientist interested in the *molecular biology of organismal interactions*. Research could include, but is not limited to, immunology, parasitology, plant-microbe interactions or symbiosis. Research conducted in an evolutionary context will be preferred. We seek a scientist who will strengthen and expand the research and teaching within the department (see www.gwu.edu/~biology). The successful candidate will develop or maintain an externally funded research program that involves graduate and undergraduate students. Teaching will include an undergraduate lab course in molecular biology and second course within the candidate's area of expertise. A completed PhD and postdoctoral experience is required. Review of applications will begin November 16, 2008 and will be ongoing until the position is filled. Application procedure: interested candidates should submit electronically 1) a letter of application, 2) a CV, 3) a brief description of research interests including past accomplishments and future plans, 4) a statement of teaching plans including a description of courses that could be taught, and 5) up to three publications. Candidates should arrange for three letters of recommendation to be sent to: Molecular Biology Search Chair Department of Biological Sciences 340 Lisner Hall 2023 G Street NW The George Washington University Washington DC 20052 e-mail: mbsearch@gwu.edu Phone: 202 994-6090 Only complete applications will be considered. GWU is an equal opportunity/affirmative action employer. The search committee seeks to attract an active, culturally and academically diverse faculty of the highest caliber. _____________________________ -- L. Courtney Smith, PhD Professor 340 Lisner Hall (mailbox) 414 Lisner Hall (office) Department of Biological Sciences George Washington University 2023 G St NW Washington DC 20052 202-994-9211; office 202-994-6100; fax www.gwu.edu/~clade/faculty/smith/ From info from noster-it.com Thu Oct 30 09:18:14 2008 From: info from noster-it.com (Dokorek) Date: Thu Oct 30 11:40:52 2008 Subject: [Drosophila] help in inserting content, build contact network Message-ID: Join the Fastest Growing Bio Community - you know Facebook, Xing or MySpace, LinkedIn. In biology it exists as well: Visit academic portal http://biospace.ethz.ch register, confirm registration in email - join group, institute (if no exist - create your institution), create own group of interesst and share data, contacts, micorscope images, build network. You can be first and can create a future scientific group of interests. ETH Zuerich Team