From zezhu from UTMB.EDU Fri Apr 10 14:57:09 2009 From: zezhu from UTMB.EDU (Zhu, Zengrong) Date: Fri Apr 10 19:19:39 2009 Subject: [Drosophila] Help! Need antibody against plasma membrane marker Message-ID: Hi, I need an antibody against plasma membrane marker. Many papers suggest Na+ K+ ATPase. Could anyone plz give me suggestion where I could buy good antibody against Na+ K+ ATPase? or Have any other ideas? Thanks -------------------------------------------------- Zengrong Zhu Room 4.508, Research Building 17 Department of Neuroscience and Cell Biology University of Texas Medical Branch at Galveston 301 University Boulevard Galveston, TX 77555 From zhuzengrong from hotmail.com Fri Apr 10 19:25:18 2009 From: zhuzengrong from hotmail.com (Zengrong Zhu) Date: Sat Apr 11 09:03:50 2009 Subject: [Drosophila] Help! Need antibody against plasma membrane marker Message-ID: Hi, I need an antibody against plasma membrane marker. Many papers suggest Na+ K+ ATPase. Could anyone plz give me suggestion where I could buy good antibody against Na+ K+ ATPase? or Have any other ideas? Thanks -------------------------------------------------- Zengrong Zhu Room 4.508, Research Building 17 Department of Neuroscience and Cell Biology University of Texas Medical Branch at Galveston 301 University Boulevard Galveston, TX 77555 From sa08383 from wotan.mdacc.tmc.edu Mon Apr 20 11:21:26 2009 From: sa08383 from wotan.mdacc.tmc.edu (Kathleen Gajewski) Date: Mon Apr 20 12:38:45 2009 Subject: [Drosophila] fly enhancer site Message-ID: <5AF5F2E0-6342-4FE6-AA9B-C383795307FF@wotan.mdacc.tmc.edu> I just noticed that my old link to the fly enhancer site ((http://flyenhancer.org/Main ) isn't working anymore. Is the site still up? If so, does anyone have the new address?? Thanks KG From pacmanfly from flypush.imgen.bcm.tmc.edu Tue Apr 21 14:05:49 2009 From: pacmanfly from flypush.imgen.bcm.tmc.edu (P[acman] Resources) Date: Thu Apr 23 19:01:26 2009 Subject: [Drosophila] P[acman] BAC libraries Message-ID: <20090421190550.058F84CCBC@flypush.imgen.bcm.tmc.edu> Dear Drosophilist, At the recent fly meeting in Chicago, we reported on two BAC libraries (a 20 kb and an 80 kb library) cloned in the P[acman] recombineering and transgenesis vector system. This new resource makes recombineering and transgenesis easier, and we believe that it will be a useful addition to the Drosophila toolkit. A manuscript describing the libraries and applications is now in press at Nature Methods and should appear online in May: "Versatile P(acman) BAC Libraries for Transgenesis Studies in Drosophila melanogaster" by Koen J. T. Venken, Joseph W. Carlson, Karen L. Schulze, Hongling Pan, Yuchun He, Rebecca Spokony, Kenneth H. Wan, Maxim Koriabine, Pieter J. de Jong, Kevin P. White, Hugo J. Bellen* and Roger A. Hoskins. To learn more about the project, go to our home page at http://www.pacmanfly.org. In addition to information on P[acman] reagents and protocols, you will find a link to a genome browser for identifying P[acman] clones containing genes and genomic regions of interest. Provide the CG number or gene symbol for your gene (wildcard * allowed), and you will find genomic clones from the two libraries that encompass the gene. Additional search options are described on the browser page. If clone coverage is limited in your region of interest, then toggle on the partially mapped clones, which are anchored to the genome by alignment of one end sequence only, for more choices. Clicking on a clone in the browser will take you to a page with the deduced sequence of the genomic insert and a link to the BACPAC Resources website, where clones can be purchased on a cost-recovery basis. *Questions about this resource should be directed to Hugo Bellen . Best, Roger Hoskins Koen Venken Hugo Bellen From littlenitnoy from yahoo.co.uk Wed Apr 22 05:01:59 2009 From: littlenitnoy from yahoo.co.uk (Indrayani Ghangrekar) Date: Thu Apr 23 19:02:10 2009 Subject: [Drosophila] Re: Fly Enhancer website address Message-ID: <582172.11739.qm@web25902.mail.ukl.yahoo.com> Hi Kathleen, I used the site recently and it has moved to http://opengenomics.org/index.php and you have to select the fly enhancer program for download (from all the other genomes supported). Hope that helps! Indi From make103080 from yahoo.com.mx Thu Apr 23 16:13:36 2009 From: make103080 from yahoo.com.mx (Miguel Angel Mendoza Ortiz) Date: Sun Apr 26 14:40:02 2009 Subject: [Drosophila] Maternal Contibution Message-ID: <515398.11178.qm@web34305.mail.mud.yahoo.com> Hello all, I am a student from Mexico working with fruit flies too. I have read the message posted in your website on November 2007 about ?getting rid of maternal contribution?? I have the same problem with getting rid of maternal contribution; I was wandering if you can help me with some ideas to reduce the maternal contribution. how can I be member? ?Obt?n la mejor experiencia en la web! Descarga gratis el nuevo Internet Explorer 8. http://downloads.yahoo.com/ieak8/?l=mx From mdushay from iit.edu Sun Apr 26 10:40:02 2009 From: mdushay from iit.edu (Mitch Dushay) Date: Sun Apr 26 14:40:33 2009 Subject: [Drosophila] half pint glass bottles Message-ID: <49F48052.9000708@iit.edu> If anyone wants half pint glass bottles of the old milk-type, I have about 210 available for the cost of shipping. -- Mitch Dushay Department of Biology Life Sciences Bldg, IIT 3101 S Dearborn St Chicago, IL 60616 Office telephone: +1-312-567-8849 From David_Rand from brown.edu Thu Apr 30 19:52:00 2009 From: David_Rand from brown.edu (David Rand) Date: Fri May 1 08:44:03 2009 Subject: [Drosophila] Postdoc: mitochondrial genetics of Drosophila Message-ID: Postdoc: Drosophila mitochondrial fitness interactions at Brown University A postdoc position is available to work on nuclear-mitochondrial fitness interactions in the laboratory of David Rand and Brown University. The general theme of this NIH funded project is to study the epistatic interactions among nuclear and mitochondrial genotypes that modulate fitness, physiological and biochemical traits in Drosophila. Mitochondrial genotypes from different strains and species of the melanogaster subgroup are placed on various nuclear chromosomal genetic backgrounds to dissect the genetic basis of life history traits. The project involves molecular and quantitative genetic assays of Drosophila strains, genetic mapping experiments, microarray and biochemical assays of specific genotypes, and inferences from molecular population genetics and evolution. Applicants must have completed their PhD before starting the position, and have a strong record of accomplishment from their dissertation work. Applicants should have some combination of experience with molecular genetic techniques, Drosophila genetics, quantitative genetics, biochemical assays, molecular evolution, computational analysis, and an interest in evolution or genetics. The appointment is open for up to 3 years, and successful candidates will receive guidance in the preparation of an NIH NRSA Fellowship to pursue related projects. The start date is spring or summer 2009. Applications will be reviewed until the position is filled. To apply please send 1) a CV, 2) a short statement of research interests and experience, including a paragraph on how your previous experience makes you well qualified for this position, and 3) contact information for three references to: David Rand email: David_Rand@brown.edu Department of Ecology and Evolutionary Biology Box G-W, 80 Waterman Street Brown University Providence, RI 02912 Voice: (401) 863-2890 (Office) (401) 863-1063 (Lab) Fax: (401) 863-2166 web pages: http://research.brown.edu/research/profile.php?id=1100924991&r=1 http://www.brown.edu/Departments/EEB/rand/index.htm The Department of Ecology and Evolutionary Biology at Brown is an interactive group with strengths that span genomics, evolutionary biology, functional morphology, ecology, and environmental science. There are close ties to the Department of Molecular and Cellular Biology, and the Center for Computational Molecular Biology, as well as the Marine Biological Laboratory in Woods Hole http://www.brown.edu/Departments/EEB/index.html http://www.brown.edu/Departments/Molecular_Biology/ http://www.brown.edu/Research/CCMB/ http://www.mbl.edu http://jbpc.mbl.edu/ http://ecosystems.mbl.edu/ Information on postdoctoral studies at Brown can be found here: http://biomed.brown.edu/grad-postdoc/ Providence is widely recognized (by Rhode Islanders) as the Venice of New England and is wonderful place to live. http://www.providenceri.com/ ---------------------------------------------------------------------------------------------- David M. Rand Professor of Biology Department of Ecology and Evolutionary Biology Box G-W, 80 Waterman Street Brown University Providence, RI 02912 Voice: (401) 863-2890 (Office - Walter Hall 202) (401) 863-1063 (Lab - BioMed Center 516-518) Fax: (401) 863-2166 email: David_Rand@brown.edu web pages: http://www.brown.edu/Departments/EEB/rand/index.htm http://research.brown.edu/research/profile.php?id=1100924991&r=1