From Sylwester.Chyb from csiro.au Thu Jun 4 20:38:33 2009 From: Sylwester.Chyb from csiro.au (Sylwester.Chyb@csiro.au) Date: Fri Jun 5 06:39:33 2009 Subject: [Drosophila] common Drosophila markers - help needed Message-ID: <4929677685F1E6458DDF5A3445976A8A33E43603@exvic-mbx05.nexus.csiro.au> Hi all, I was wondering what would be the most common Drosophila markers used in your research - the obvious choices would be: w, B, Cy, D, Tb, Sb, Ser, sn, y, e, Sco, Sp, f, Ubx, ey, Hw Please add to the above list, preferably along with a short comment about the phenotype. Your help will be much appreciated. Cheers, Sylwek From mitch4chicago from gmail.com Sat Jun 6 07:32:32 2009 From: mitch4chicago from gmail.com (Mitch) Date: Sat Jun 6 07:38:31 2009 Subject: [Drosophila] spinsect insect trap wanted Message-ID: <50ec4fa40906060532g3df640f0m793b68aac3231628@mail.gmail.com> Does anyone have a functioning Spinsect insect trap from Spinsect Corp in Ohio gathering dust? I'd like to put one of these oldtimers in my laboratory. Please email me either here at mitch4chicago@gmail.com or mdushay@iit.eduThank you! Mitch From mckittrickr from yahoo.com Sat Jun 6 08:42:56 2009 From: mckittrickr from yahoo.com (mckittrickr@yahoo.com) Date: Sat Jun 6 10:13:51 2009 Subject: [Drosophila] Re: half pint glass bottles References: Message-ID: On Apr 26, 10:40?am, Mitch Dushay wrote: > If anyone wants half pint glass bottles of the old milk-type, I have > about 210 available for the cost of shipping. > -- > Mitch Dushay > Department of Biology > Life Sciences Bldg, IIT > 3101 S Dearborn St > Chicago, IL ?60616 ? ? ? Office telephone: +1-312-567-8849 Do you still have the bottles? From widdisetiawan from yahoo.com Sat Jun 6 08:54:41 2009 From: widdisetiawan from yahoo.com (Widdi Setiawan) Date: Sat Jun 6 18:03:15 2009 Subject: [Drosophila] can help me Message-ID: <87905.49894.qm@web111607.mail.gq1.yahoo.com> do you can help me......iam from indonesia university of jendral soedirman....i need all picture mutan of drosophila...can you give me its....thanks From vlloyd from mta.ca Thu Jun 11 11:44:35 2009 From: vlloyd from mta.ca (Vett Lloyd) Date: Thu Jun 11 14:17:22 2009 Subject: [Drosophila] commercial inverse PCR service Message-ID: Hello, Does anyone know of a commercial provider of inverse PCR to map P-element insert sites? Thanks, Vett -- Dr. Vett Lloyd Dept. Biology Mt. Allison University 63B York St. Sackville NB E4L 1G7 Canada fax 506 364 2505 phone 506 364 2500 www.Drosophiloid.ca From sa08383 from wotan.mdacc.tmc.edu Thu Jun 11 18:44:08 2009 From: sa08383 from wotan.mdacc.tmc.edu (Kathleen Gajewski) Date: Thu Jun 11 20:45:01 2009 Subject: [Drosophila] Looking for balancers Message-ID: <1F615504-4CC3-426C-8CAF-4678DE283EE0@wotan.mdacc.tmc.edu> I;m looking for these balancers: 1) a CyO or SM6 with a UAS-GFP (and no GAL4) 2) Any 2nd chromosome balancer with an RFP marker 3) Any 3rd chromosome balancer with an RFP marker Does anyone out in flyland have any of these? Thanks KG From rachel from flymine.org Mon Jun 15 09:18:36 2009 From: rachel from flymine.org (Rachel Lyne) Date: Mon Jun 15 10:38:11 2009 Subject: [Drosophila] FlyMine version 17.0 released Message-ID: <4A36583C.8080504@flymine.org> We have released version 17.0 of FlyMine. In this release data from FlyBase has been updated to release FB2009_05 and the D. melanogaster natural transposable elements have been added. Many other sources have been updated to the latest versions including seven new tissues for the FlyAtlas expression data. Interface improvements include autocompletion of GO terms in the query builder and links from list analysis pages and report pages to modMine. For a list of all changes see www.flymine.org/release-notes. For a full list of data sources see www.flymine.org/release-17.0/dataCategories.do For all types of feedback email support@flymine.org The FlyMine Team - About FlyMine - FlyMine is an integrated database of genomic, expression and protein data from Drosophila, Anopheles and C. elegans. As an integrated resource it is possible to run data mining queries that span domains of biological knowledge. FlyMine is a freely available resource. ----------------- From herogene from gmail.com Thu Jun 18 07:36:31 2009 From: herogene from gmail.com (Herojeet S) Date: Thu Jun 18 08:46:54 2009 Subject: [Drosophila] Re: Looking for balancers References: Message-ID: On Jun 12, 4:44?am, Kathleen Gajewski wrote: > I;m looking for these balancers: > ? 1) a CyO or SM6 with a UAS-GFP (and no GAL4) > ? 2) Any 2nd chromosome balancer with an RFP marker > ? 3) Any 3rd chromosome balancer with an RFP marker > > Does anyone out in flyland have any of these? > > Thanks > > KG Hi Kathleen, CyO with UAS-lacZ insertion has been reported by Yoshihara and Ito, 2000, D. I. S. 83: 199--202. But, balancers with UAS-GFP has not so far been reported (to my knowledge). Wishes, Herojeet From anna.hitrik from weizmann.ac.il Fri Jun 19 04:14:04 2009 From: anna.hitrik from weizmann.ac.il (Anna Hitrik) Date: Fri Jun 19 09:30:06 2009 Subject: [Drosophila] Ovary Cryosections Message-ID: <21015243.1245402844544.JavaMail.oracle@colibri.weizmann.ac.il> Dear community, ? I am trying to perform cryosections of Drosophila ovary. I am doing paraformaldehyde fixation before freezing the ovaries in O.C.T. and then cut the frozen?tissue to 7 micron sections. The aim is to extract RNA from the single cells (Laser Capture Microscopy). The problem is that I can not obtain the sutisfying morphology of the sections and can not distinguish between cells. Does anyone have any suggestions for improving the morphology? ? Thank you in advance, ? Anna Hitrik ? From cristiancorio from gmail.com Mon Jun 22 08:25:42 2009 From: cristiancorio from gmail.com (cris corio) Date: Mon Jun 22 09:01:17 2009 Subject: [Drosophila] Re: Dros Digest, Vol 50, Issue 7 In-Reply-To: <200906191703.n5JH3Wp18690@net.bio.net> References: <200906191703.n5JH3Wp18690@net.bio.net> Message-ID: <74da48020906220625n5950a148ma08639b08b66ea9@mail.gmail.com> Dear Anna, I am using AFA for diferent micoscopy tecnics (In toto inmuno-histochemistry and resin inclusion for conventional staining) and in both cases i recomend this fixation mixture where hight preservation morphology is needed. Besides im not sure about the AFA cross-linking effects in RNA, but i think is not larger than paraformaldehyde. I hope to be useful 2009/6/19 > Send Dros mailing list submissions to > dros@net.bio.net > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/dros > or, via email, send a message with subject or body 'help' to > dros-request@net.bio.net > > You can reach the person managing the list at > dros-owner@net.bio.net > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Dros digest..." > > > Today's Topics: > > 1. Ovary Cryosections (Anna Hitrik) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 19 Jun 2009 12:14:04 +0300 (IDT) > From: Anna Hitrik > Subject: [Drosophila] Ovary Cryosections > To: Dros@magpie.bio.indiana.edu > Message-ID: > <21015243.1245402844544.JavaMail.oracle@colibri.weizmann.ac.il> > Content-Type: text/plain;charset=ISO-8859-1 > > Dear community, > > I am trying to perform cryosections of Drosophila ovary. I am doing > paraformaldehyde fixation before freezing the ovaries in O.C.T. and then cut > the frozen tissue to 7 micron sections. The aim is to extract RNA from the > single cells (Laser Capture Microscopy). The problem is that I can not > obtain the sutisfying morphology of the sections and can not distinguish > between cells. Does anyone have any suggestions for improving the > morphology? > > Thank you in advance, > > Anna Hitrik > > > > ------------------------------ > > _______________________________________________ > Dros mailing list > Dros@net.bio.net > http://www.bio.net/biomail/listinfo/dros > > End of Dros Digest, Vol 50, Issue 7 > *********************************** > From ktokuyasu from ucsd.edu Mon Jun 22 16:51:53 2009 From: ktokuyasu from ucsd.edu (Kiyoteru Tokuyasu) Date: Tue Jun 23 07:11:18 2009 Subject: [Drosophila] cryosections for laser capture microscopy Message-ID: <30177ABB-2320-4005-8384-DBF94632B224@ucsd.edu> Dear Dr. Anna Hitrik, Dan forwarded your mail to me. Let me describe my opinion on your problem. First, I would like to point out that components of O.C.T. are polymers, which means that it may fill in intercellular spaces but not penetrate into the cell interiors through intact cell membrane. Hence, when the ovary is frozen, ice crystals will be formed inside the cells, damaging the cellular structures, and as the sections are thawed, artificial spaces will be formed in the cytoplasm. Secondly, as you know, cell structures are more difficult to recognize in thicker sections than in thin ones, mainly due to super-positions. This will be particularly true in unstained sections. If cryosections thinner than 7 micron thickness are OK or preferable, there is a way to obtain 0.1-2 micron thick cryosections rather readily. The cutting quality of thin cryosections is much improved by infusing a sucrose solution into cells (Tokuyasu, K.T. 1973. A technique for ultracryotomy of cell suspensions and tissues. J. Cell Biol. 57:551-565). A formaldehyde-fixed Drosophila ovary may be infused with 1.5-2.3M sucrose in an appropriate buffer, frozen by plunging the specimen into liquid nitrogen and sectioned at -70~ -90oC. If your cryo-microtome is not suitable for cutting such thin cryosections, you may try to find a nearby EM lab equipped with an ultra-cryomicrotome and carrying out immunostaining of ultrathin cryosections. Such lab will know how to retrieve cryosections onto glass slides and remove sucrose from the sections. You may then have a fair chance to see cell boundaries in thin, thawed cryosections, with phase-contrast LM or other means. If your project requires to see much finer structural details, I would propose a new approach; all operations involving the laser capture microscopy are done first to obtain a data “map” and then the cryosection is stained to obtain a morphological “map”. Using an appropriate coordinate, one may combine the two “maps” to obtain the whole “map”. Such an approach could also be used for your 7 micron thick section, if necessary. I would hope that these suggestions were useful to you. Sincerely, K. Tokuyasu From sara.silva from ibmc.up.pt Tue Jun 23 10:36:13 2009 From: sara.silva from ibmc.up.pt (Sara Silva) Date: Tue Jun 23 11:25:31 2009 Subject: [Drosophila] FRT proximal to 42A Message-ID: <12D41178-C970-406D-BDD4-9E9BB828E05C@ibmc.up.pt> Dear all, Does anyone have a Drosophila stock with FRT sites which are centromere proximal to 42A? Could you please contact me on sara.silva@ibmc.up.pt if you have it? Thank you very much, Sara From crichards from mdanderson.org Tue Jun 23 15:35:53 2009 From: crichards from mdanderson.org (Richards,Clare B) Date: Tue Jun 23 15:39:10 2009 Subject: [Drosophila] volume injected in germline transformation Message-ID: <5E2A85A9673FB74896125C8640DDC9741FAE89B9A1@DCPWVMBXC0VS1.mdanderson.edu> Can anyone tell me the volume of liquid usually injected into the fly in a germline transformation? From francisco.useche from gmail.com Tue Jun 23 15:08:26 2009 From: francisco.useche from gmail.com (Francisco Useche) Date: Tue Jun 23 15:39:43 2009 Subject: [Drosophila] CG ids Message-ID: Hi, Can anybody let me know the origin of the CG ids for drosophila? Do they come from another drosophila db or sequencing project? Thanks, Francisco From heterochromatic from gmail.com Thu Jun 25 13:29:12 2009 From: heterochromatic from gmail.com (sandra schulze) Date: Thu Jun 25 13:33:02 2009 Subject: [Drosophila] safety issues Message-ID: <5b26f17e0906251129t175607b6n1b6f45300d46851c@mail.gmail.com> OK (deep breath) I am facing down bureaucrats at my school that need language that calms them down about working with transgenic fruit flies. I have already read the 132 (!!!$&^%*$^%&*) page NIH Guidelines and have confirmed that I am BL1 (biolevel safety 1) working with an insect model that is largely exempt from lots of things (no toxins, no infectious agents, no poisons, no horizontally transmissable anything etc). However the mere fact that they have transgenes means I have to report to a safety committee here. But I need language to convince the safety committee that fruitflies are safe, that disposal by freezing is JUST FINE, that they are crippled and if released accidentally pose no threat etc etc etc. Can anyone give me some language, especially concerning routine stock maintenance (disposal especially) that will make bureaucrats happy??? I am totally responsible for whatever I write, I just would love some language that will help the bureaucrats understand. I have a headache. Sandra Schulze