From sian from morgan.harvard.edu Thu Nov 5 16:16:04 2009 From: sian from morgan.harvard.edu (L. Sian Gramates) Date: Thu Nov 5 16:19:02 2009 Subject: [Drosophila] FlyBase Genome Database Curator/Annotator Position at Harvard University Message-ID: <4AF34094.8090102@morgan.harvard.edu> Genome Database Curator/Annotator, Biological Laboratories, Harvard University FlyBase seeks a full-time Ph.D.-level scientific curator/annotator to join the FlyBase team in the Dept. of Molecular & Cellular Biology at Harvard University, Cambridge, under the direction of Prof. William Gelbart. This is a full-time staff position. Job description: Curational responsibilities at the Harvard site include the scanning and abstracting of analysis of current literature on the molecular biology of Drosophila, annotation and analysis of the Drosophila melanogaster genome, coordination of the inclusion of high- throughput molecular datasets into FlyBase, and design of improved interfaces for providing molecular data to the user scientific research community. Curators work as a part of a team, interacting with biologists at other FlyBase sites, and coordinating with software engineers to develop and improve software. Curators also interface with the scientific community, assisting users on-line and at research conference workshops. FlyBase is constantly evolving, seeking both to improve and to expand its role within the Drosophila and wider scientific communities. The ideal applicant will be enthusiastic about participating in this process, bringing to the FlyBase group expertise and ideas concerning emerging directions in Drosophila biology and genomic/proteomic analysis. Required Education, Experience, Skills: Ph.D. in molecular biology or genetics. Experience with molecular genetics and Drosophila biology are essential. To apply: Please contact William Gelbart (gelbart@morgan.harvard.edu), and include a CV, a description of research experience and names of three references. Please cc your communication to the senior curator, Dr. Beverley Matthews (bmatthew@morgan.harvard.edu). From events from embl-events.de Fri Nov 13 10:49:22 2009 From: events from embl-events.de (EMBL Courses & Conferences) Date: Fri Nov 13 11:57:43 2009 Subject: [Drosophila] Visualizing Biological Data, 3-5 March 2010: Travel fellowships avaliable Message-ID: <0M944P-1MwYB03odB-00CBVj@mrelayeu.kundenserver.de> Dear colleagues, We would like to remind you that November 16 is the deadline for registration and abstract submission for the first EMBO Workshop on Visualizing Biological Data (VizBi, http://www.vizbi.org), 3-5 March 2010, at EMBL Heidelberg, Germany. The goal of the workshop is to bring together, for the first time, researchers developing and using visualization systems across all areas of biology, including genomics, sequence analysis, macromolecular structures, systems biology, and imaging (including microscopy and magnetic resonance imaging). The workshop program includes high-profile speakers from each of these areas, as well as keynote talks by Ben Fry (Seed Visualization), Bang Wong (Broad Institute) and Chris North (Virginia Tech) - for details, see http://tinyurl.com/vizbi10Speakers. We are pleased to announce that we have a limited number of student travel fellowships provided by the European Science Foundation - see http://www.vizbi.org for details on how to apply. Furthermore, a 15% discount on the registration fee is available to members of the International Society for Computational Biology (ISCB). To apply to join the workshop, please go to http://www.vizbi.org and submit an abstract and image related to your work. In addition to the program of speakers, participants are normally required to present a poster and to give a 'fast-forward' presentation (limited to 30 seconds and 1 slide). If several people are working on the same project they may use the same abstract to apply. However, we encourage registrants to submit work that reflects their own interests - and not necessarily new or unpublished work. But, since places are limited, we will use submissions to select participants whose work is most relevant to the goals of the workshop. Notifications of acceptance will be sent within three weeks after the close of submissions. You may also submit your image for consideration for the VizBi Scientific Art prize. This prize will be awarded to the submitted image that best conveys a strong scientific message in a visually compelling manner. Please forward this announcement to anyone who may be interested. We hope to see you in Heidelberg next spring! Seán O'Donoghue, EMBL Jim Procter, University of Dundee Nils Gehlenborg, European Bioinformatics Institute Reinhard Schneider, EMBL If you have any questions about the registration process please contact: Adela Valceanu Conference Officer European Molecular Biology Laboratory Meyerhofstr. 1 D-69117 Heidelberg Tel: +49-6221-387 8625 Fax: +49-6221-387 8158 Email: valceanu@embl.de For full event listings please visit our website: www.embl.org/events or sign up for our newsletterwww.embl.de/events/newsletter From cscmw from yahoo.com.cn Sat Nov 14 02:46:23 2009 From: cscmw from yahoo.com.cn (chao) Date: Sat Nov 14 14:38:34 2009 Subject: [Drosophila] Can anyone know how to prove my shibire[ts] by behavioral test Message-ID: <419136.27365.qm@web15101.mail.cnb.yahoo.com> Dear all, ? can anyone know how to test if my shibire-ts works or not by by obserbing behavior of flies on heatshock? ? Thanks in advance. ? Chao ___________________________________________________________ ????????????????? http://card.mail.cn.yahoo.com/ From Robert.Maeda from unige.ch Mon Nov 16 05:42:53 2009 From: Robert.Maeda from unige.ch (Rob Maeda) Date: Mon Nov 16 09:49:41 2009 Subject: [Drosophila] finding BACs from other Drosophila species Message-ID: <382DEA12-EF1A-4182-BB1A-8C13A3D6E31F@unige.ch> I am trying obtain BACs from Drosophila pseudoobscura. I know we can order them from the BAC center (CHORI), but what I can't figure out is how one translates a genomic region to a BAC number. For melanogaster, one can just look at the gene region and the BACs covering it are listed or shown on screen. This does not seem to be the case for the other species. Can anyone tell me if there is an easy way to do this for the other sequenced species? Thanks a lot, Rob Maeda University of Geneva Dept of Zoology and Animal Biology robert.maeda@unige.ch From stephanie_mohr from hms.harvard.edu Mon Nov 16 15:23:44 2009 From: stephanie_mohr from hms.harvard.edu (Stephanie Mohr) Date: Mon Nov 16 17:20:49 2009 Subject: [Drosophila] finding BACs from other Drosophila species In-Reply-To: <382DEA12-EF1A-4182-BB1A-8C13A3D6E31F@unige.ch> References: <382DEA12-EF1A-4182-BB1A-8C13A3D6E31F@unige.ch> Message-ID: <1ADCDB32-3A9C-4AE8-8462-65FABB1964DB@hms.harvard.edu> Matt Booker at the Drosophila RNAi Screening Center (DRSC) wrote a tool for looking up genomic information for non-mel species, including pseudoobscura. I think this can help you to identify the BACs of interest. http://www.flyrnai.org/cgi-bin/RNAi_find_rescue_compl.pl Please feel free to contact Matt if you have any questions about the on-line tool or suggestions for improvement. - Stephanie Mohr. On Nov 16, 2009, at 5:42 AM, Rob Maeda wrote: > I am trying obtain BACs from Drosophila pseudoobscura. I know we can > order them from the BAC center (CHORI), but what I can't figure out is > how one translates a genomic region to a BAC number. For > melanogaster, one can just look at the gene region and the BACs > covering it are listed or shown on screen. This does not seem to be > the case for the other species. Can anyone tell me if there is an > easy way to do this for the other sequenced species? > > Thanks a lot, > > Rob Maeda > University of Geneva > Dept of Zoology and Animal Biology > robert.maeda@unige.ch > > _______________________________________________ > Dros mailing list > Dros@net.bio.net > http://www.bio.net/biomail/listinfo/dros From amiramenfis from gmail.com Tue Nov 17 11:25:52 2009 From: amiramenfis from gmail.com (amira menfis) Date: Tue Nov 17 11:51:59 2009 Subject: [Drosophila] yeast two-hybrid Message-ID: <3210a0970911170825h6581b2a0h7df0839fa3edf835@mail.gmail.com> Hi all, Does anybody know how to get a library of drosophila testis or male drosophila for yeast two-hybrid? Thanks a lot! From bilder from berkeley.edu Fri Nov 20 11:24:47 2009 From: bilder from berkeley.edu (David Bilder) Date: Fri Nov 20 12:57:50 2009 Subject: [Drosophila] Tech position: UC-Berkeley Message-ID: A research technician position is available in the Bilder lab (http://mcb.berkeley.edu/labs/bilder/) at the University of California-Berkeley, studying epithelial morphogenesis and tumor suppression. The successful applicant will carry out genetic, molecular biological and microscopy-based experiments in research studying fundamental questions of cell biology and development. The following qualifications are absolutely required: -Bachelor's Degree in Molecular Biology, Genetics, Biochemistry or related discipline. -One or more years of laboratory experience involving techniques listed above. -Previous experience with Drosophila or other genetic organism. -Demonstrated attention to detail; strong organizational and problem-solving skills; the ability to communicate effectively. The position has a two-year term, and is ideal for applicants interested in gaining research experience prior to entering a graduate degree program. To apply, send a cover letter describing previous experience, CV, and the names of three references to jschoenfeld@berkeley.edu. From Robin_Brese from brown.edu Mon Nov 23 10:18:27 2009 From: Robin_Brese from brown.edu (Brese, Robin) Date: Tue Nov 24 09:29:19 2009 Subject: [Drosophila] cleaning glass vials - help! Message-ID: Hello, I am having quite a bit of trouble cleaning glass fly vials. My current procedure is as follows: autoclave, pull plugs, rinse, wash. The trouble is that our washer does not remove the pupal cases. I have tried changing the cycle numerous times and am going to try using a different soap. Nothing seems to work though. If anyone could please let me know how they wash glass vials at their facility and what kind of washer they use, I would really appreciate it. THANKS! Best regards, Robin Robin L. Brese Research Assistant Department of Molecular Biology, Cell Biology, and Biochemistry Brown University Box G-L3 Providence, RI 02912 (401) 863-7365 Robin_Brese@brown.edu From kercook from indiana.edu Tue Nov 24 09:51:44 2009 From: kercook from indiana.edu (Kevin Cook) Date: Wed Nov 25 09:54:14 2009 Subject: [Drosophila] cleaning glass vials - help! Message-ID: <4B0BF300.409@indiana.edu> Hi Robin-- We use a high pH cleaning solution. It's mostly Potassium hydroxide. Our local manufacturer is Madison Chemical and the cleaner is called Challenge. There is a lot of info on their website (http://www.madchem.com). A lot of companies make similar cleaners, because they're used extensively for "Clean In Place" (CIP) applications, such as cleaning dairy equipment without disassembly. You shouldn't have too much trouble identifying something similar if you want to buy locally. With most dishwashers, I think you'll need a fairly long wash time to get vials clean. Good luck, Kevin -- Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology Indiana University 1001 E. Third St. Bloomington, IN 47405-7005 kercook@indiana.edu 812-856-1213 (office), 812-855-2577 (fax) http://flystocks.bio.indiana.edu