From martinoneil95 from yahoo.no  Mon Oct 13 13:18:04 2008
From: martinoneil95 from yahoo.no (Martin Oneil)
Date: Mon Oct 13 19:23:44 2008
Subject: [Fluorescent-proteins] Advice on mammalian cells
Message-ID: <883116.82188.qm@web28316.mail.ukl.yahoo.com>

We would like to do YFP localization and BiFC assays in a mammalian cell line. Does anyone have any recommendations on with cell lines to buy that are easy to transfect and image using confocal microscopy?

Thank you in advance for your time


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From novalidaddress from nurfuerspam.de  Tue Oct 14 03:24:18 2008
From: novalidaddress from nurfuerspam.de (WS)
Date: Tue Oct 14 11:25:30 2008
Subject: [Fluorescent-proteins] Re: Advice on mammalian cells
References: <mailman.336.1223943848.29717.fluorpro@net.bio.net>
Message-ID: <7ddfd7c1-7bf3-4c60-a686-6be83c1433fc@k13g2000hse.googlegroups.com>

Hi,

in my experience, 3T3 mouse fibroblasts are easy to culture, easy to
transfect and give nice pictures. They should be easy to obtain, maybe
you even know a lab doing cell culture, they might have them.

Wo