From owner-7tms_r@net.bio.net Sun Apr 02 23:00:00 1995 Newsgroups: bionet.molbio.proteins.7tms_r Path: biosci!bloom-beacon.mit.edu!spool.mu.edu!howland.reston.ans.net!pipex!oleane!jussieu.fr!univ-lyon1.fr!swidir.switch.ch!scsing.switch.ch!rzusuntk.unizh.ch!NewsWatcher!user From: maga@vetbio.unizh.ch (Giovanni Maga) Subject: D2O exchange for proteins Message-ID: Followup-To: bionet.molbio.proteins.7tms_r Sender: newsadm@rzu-news.unizh.ch (CNEWS ADMINISTRATION) Organization: University of Zurich Irchel- Biochemistry Date: Mon, 3 Apr 1995 21:15:53 GMT Lines: 19 Dear bionetters, we want to begin to study a small protein (30kD, pI around 5) by neutron scattering. We can purify such protein in mg amounts at homogenity, However, we need to provide it in D2O rather than that in H2O for neutron scattering analysis. We tried once by liophylizing our protein and then resuspending it in phosphate buffer at pH 6 in D2O, but we had serious troubles in getting it soluble again. Does anybody know a good method to exchange H2O buffers with D2O buffers? Is the pH important in this reaction (well, the pD in this case)? Any buffering system will work or some is preferred? I tried to get advice by reading a couple of issues of Methods in Enzymology which were dealing with nmr studies, but I didn't find an answer to my (basic, I know) questions. Any help will be greatly appreciated. Answer here or e-mail to: maga@vetbio.unizh.ch G.Maga,PhD Institute of Veterinary Biochemistry University of Zuerich-CH From owner-7tms_r@net.bio.net Mon Apr 03 23:00:00 1995 Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins.7tms_r Path: biosci!bcm!cs.utexas.edu!swrinde!pipex!sunic!sunic.sunet.se!news.uni-c.dk!novo.dk!news From: ces@novo.dk (Carsten E. Stidsen) Subject: HEK293 expression of receptors Message-ID: <1995Apr3.124021.9781@novo.dk> Sender: news@novo.dk (USENET News System) Nntp-Posting-Host: pcces.novo.dk Organization: Novo Nordisk A/S, Denmark X-Newsreader: WinVN 0.99.2 Mime-Version: 1.0 Date: Mon, 3 Apr 1995 12:40:21 GMT Lines: 24 Xref: biosci bionet.molbio.methds-reagnts:26790 bionet.molbio.proteins.7tms_r:218 Hi netters: Recently, I realized that the HEK 293 cell line (ATCC CRL-1573) is hypotriploid with approx. 30% triploid cells and 4.2% of higher ploidity. When using this cell line for permanent expression of receptors what would the likelihood be that you over time will get a cell line with another phenotype than the clone you initially picked? Would it be fair to compare ligand binding and functional properties of mutant or chimeric receptors at different passage # in this cell line? Please post your ideas, feelings or experiences on this matter to me. -- Carsten E. Stidsen, Ph.D. Department of Molecular Pharmacology Health Care Pharmaceutical Biotechnology Novo Nordisk A/S Novo Alle 1CS.23 DK-2880 Bagsvaerd Phone: +45 4442 2578 Fax: +45 4442 6920 E-mail: ces@novo.dk From owner-7tms_r@net.bio.net Tue Apr 04 23:00:00 1995 Newsgroups: bionet.molbio.proteins.7tms_r,bionet.molbio.methds-reagnts Path: biosci!daresbury!trane.uninett.no!sunic!sunic.sunet.se!news.uni-c.dk!novo.dk!news From: ces@novo.dk (Carsten E. Stidsen) Subject: HEK 293 expression of receptors Message-ID: <1995Apr4.064402.21997@novo.dk> Sender: news@novo.dk (USENET News System) Nntp-Posting-Host: pcces.novo.dk Organization: Novo Nordisk A/S, Denmark X-Newsreader: WinVN 0.99.2 Mime-Version: 1.0 Date: Tue, 4 Apr 1995 06:44:02 GMT Lines: 19 Xref: biosci bionet.molbio.proteins.7tms_r:219 bionet.molbio.methds-reagnts:26850 Hi netters: Recently, I realized that the HEK 293 cell line (ATCC CRL-1573) is hypotriploid with approx. 30% triploid cells and 4.2% of higher ploidity. When using this cell line for permanent expression of receptors what would the likelihood be that you over time will get a cell line with another phenotype than the clone you initially picked? Would it be fair to compare ligand binding and functional properties of mutant or chimeric receptors at different passage # in this cell line? Please post your ideas, feelings or experiences on this matter to me. -- Carsten E. Stidsen, Ph.D. Novo Nordisk A/S Novo Alle 1CS.36 DK-2880 Bagsvaerd Phone: +45 4442 2578 Fax: +45 4498 5007 From owner-7tms_r@net.bio.net Sun Apr 09 23:00:00 1995 Path: biosci!WELCHLINK.WELCH.JHU.EDU!mlevine From: mlevine@WELCHLINK.WELCH.JHU.EDU (MICHAEL A LEVINE) Newsgroups: bionet.molbio.proteins.7tms_r Subject: basal adenylyl cyclase Date: 10 Apr 1995 13:20:42 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I am trying to do some experiments using a non-hydrolyzable GTP analog and various receptor agonists to examine G protein GTP/GDP exchange, but I am troubled by a high basal level of hormone-independent GTP exchange. I have tried low magnesium and low temperature, but I don't seem to be able to improve my signal-to-noise ratio appreciably. Any suggestions on how to decrease hormone-independent GN exchange? Michael Levine Johns Hopkins/Division of Endocrinology mlevine@welchlink.welch.jhu.edu From owner-7tms_r@net.bio.net Tue Apr 11 23:00:00 1995 Path: biosci!rutgers!gatech!howland.reston.ans.net!newsserver.jvnc.net!synapse.bms.com!riversend.bms.com!user From: Roberts_Daniel_g.PRILVMS3@msmail.bms.com (Dan Roberts) Newsgroups: bionet.molbio.proteins.7tms_r,bionet.general Subject: Help needed w/ SF9 and Baculovirus cell Proteins Date: Wed, 12 Apr 1995 15:29:01 -0500 Organization: BMS Lines: 12 Message-ID: NNTP-Posting-Host: riversend.bms.com Xref: biosci bionet.molbio.proteins.7tms_r:221 bionet.general:14615 -- Netters.. I was trying to put together a list of know expressed proteins and their MW from SF9 and Baculovirus cells. I tried locating a source on Internet that would help me out but I am having no luck. Anyone have any ideas?? thanks..Dan -- Dan Roberts BRISTOL-MYERS SQUIBB PHARM. RES. CENTER "Nature is last at Bats" PRINCETON, NEW JERSEY U.S.A. PLANET EARTH, MILKY-WAY GALAXY <<<>>>Roberts_Daniel_G.PriLVMS3@MSMAIL.BMS.COM From owner-7tms_r@net.bio.net Wed Apr 12 23:00:00 1995 Path: biosci!WELCHLINK.WELCH.JHU.EDU!jbathon From: jbathon@WELCHLINK.WELCH.JHU.EDU ("JOAN M. BATHON") Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe Date: 13 Apr 1995 09:44:48 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I would like to subscribe to this service. Thanks. Joan Bathon, M.D., Associate Professor of Medicine, Johns Hopkins Univ. From owner-7tms_r@net.bio.net Thu Apr 13 23:00:00 1995 Path: biosci!rutgers!uwm.edu!cs.utexas.edu!howland.reston.ans.net!newsjunkie.ans.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: ama777@aol.com (AMA777) Newsgroups: bionet.molbio.proteins.7tms_r Subject: GTPase-activating protein Date: 15 Apr 1995 01:04:15 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 8 Sender: root@newsbf02.news.aol.com Message-ID: <3mnk4f$84m@newsbf02.news.aol.com> Reply-To: ama777@aol.com (AMA777) NNTP-Posting-Host: newsbf02.mail.aol.com I'm interested in obtaining a clone of the GTPase-activating protein gene (GAP)- mouse or human- in order to perform signal transduction experiments in tissue culture. I've attempted to purify the 3 kb gene using PCR but had no success. I would be very grateful if someone could provide me with a clone or tell me where I can obtain one. Thanks, Amer From owner-7tms_r@net.bio.net Mon Apr 17 23:00:00 1995 Path: biosci!rutgers!uwm.edu!psuvax1!news.cc.swarthmore.edu!netnews.upenn.edu!cronkite.ocis.temple.edu!VM.TEMPLE.EDU!SHICKLEY From: SHICKLEY@VM.TEMPLE.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: CFA: Phila. Chapt. Soc. for Neuroscience Date: Tue, 18 Apr 95 14:19:39 EDT Organization: Temple University Lines: 81 Message-ID: <17384C97BS86.SHICKLEY@VM.TEMPLE.EDU> NNTP-Posting-Host: vm.temple.edu <<<<<<<<<<<< MEETING ANNOUNCEMENT AND CALL FOR ABSTRACTS >>>>>>>> ANNUAL MEETING The Philadelphia Chapter of the Society for Neuroscience Friday May 12, 1995 Noon -- 6:00PM Benjamin Franklin Hall American Philosophical Society 427 Chestnut Street Philadelphia, Pennsylvania Preliminary Program (times are approximate): 12:00 - Registration ($10 in advance or at meeting) Exhibits by Corporate Sponsors: Leica Oncor Imaging VWR Scientific Pharmacia Biotech Poster Setup Begin Judging for Eliot Stellar Awards for Neuroscience: Best Undergraduate Research: $100 Best Graduate Research: $100 Best Post-Doctoral Research: $100 3:00 - 2nd Annual Eliot Stellar Awards for Neuroscience 3:30 - Business Meeting, Philadelphia Chapter, Society for Neuroscience. Election of Officers for 1995-1996 3:45 - The Annual Solomon D. Erulkar Memorial Lecture [Sponsored by the Grass Traveling Scientist Program] Introduction: Dr. John O'neil Professor Emeritus of Pharmacology Temple University School of Medicine Personal Comments: Mathew Erulkar Lecture: Dr. Eric Kandel Columbia University Title: Genes, Synapses and Long-Term Memory 5:00-6:00 Informal Reception --------------------------------------------------------------------- Abstract Information: In order to encourage interaction of Philadelphia area neuroscientists, we invite participation in the poster session. You may present an abstract from a previous meeting as many from the Philadelphia area did not get to see it at the larger national meeting. We especially encourage students, undergraduate and graduate, and post-doctoral fellows to participate. As a further inducement, we encourage participation in the Eliot Stellar Awards for Neuroscience Research which are $100 prizes in each of the following catagories: Undergraduate research; Graduate research;and Post-doctoral research. Registration for the meeting is $10 payable in advance or at the meeting. Checks should be made out to The Philadelphia Chapter of the Soc. for Neuroscience and sent to the contact person below. Abstracts may be submitted by mail, FAX or by e-mail to the address below. NOTE: Registration for the meeting is required for attending the lecture by Dr. Kandel. Contact Person: Dr. Tim Shickley Dept. of Anatomy and Cell Biology Temple University School of Medicine 3400 North Broad Street Philadelphia, PA 19140 Voice: (215) 707-2920 FAX: (215) 707-2966 shickley@vm.temple.edu _______________________________________________________________________ From owner-7tms_r@net.bio.net Thu Apr 20 23:00:00 1995 Path: biosci!RECEPTOR.MGH.HARVARD.EDU!lfk From: lfk@RECEPTOR.MGH.HARVARD.EDU (Lee F. (Frank) Kolakowski) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Receptor Family Nomenclature Date: 21 Apr 1995 11:39:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 35 Sender: daemon@net.bio.net Distribution: world Message-ID: <9504211832.AA05069@receptor> References: NNTP-Posting-Host: net.bio.net This is an interesting discussion point as there is no consensus on receptor family nomenclature. Kevin Lynch and I have a draft of a manuscript which utilizes the following nomenclature: Family A: The Rhodopsin/beta 2 Adrenergic Receptor Family Family B: The CT/PTH/Secretin Receptor Family Family C: The Metabotropic Glutamate/Calcium Sensor Family Family D: STE2 fungal pheromone receptor family Family E: STE3 fungal pheromone receptor family Family F: cAMP Receptor Family GCRDb also uses this nomenclature. Publication-wise the Secretin receptor was published first in Family B. Followed simultaneously by the CT and PTH/PTHrP receptor papers. The term the VIP family, I think is way off base despite what the Watson/Arkinstall book suggests. This is really something that should be discussed especially as the relationships among these G-protein--coupled receptor families are unclear at best, and non-existent at worst. Frank Kolakowski Email: lfk@receptor.mgh.harvard.edu 617-355-7515 (LAB) GCRDb-WWW From owner-7tms_r@net.bio.net Thu Apr 20 23:00:00 1995 Path: biosci!ITSA.UCSF.EDU!sredhar From: sredhar@ITSA.UCSF.EDU (Sunil Sreedharan) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: (none) Date: 21 Apr 1995 13:29:47 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 37 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net Sunil P. Sreedharan, Ph.D. Assistant Professor, UCSF Div. of Allergy & Immunology Dept. of Medicine, UB8B San Francisco, CA 94143-0711 Tel. (415)476-9895 FAX: (415)476-6915 email: sredhar@itsa.ucsf.edu On 21 Apr 1995, Ted Usdin wrote: > Is there a consensus on how to refer to the subfamily of receptors which > includes the receptors for secretin, VIP, PACAP, glucagon, GLP-I, PTH, > calcitonin, CRF...? > This is an area of discussion long overdue. I believe that Frank Kolakowski's nomenclature is quite reasonable for the broad division of these GPR's. My own "beef" is with the subfamily of receptors that include PACAP, VIP and secretin. Since one common feature for all 4 receptors (2 subtypes for VIP) is that they all recognize VIP, albeit with differing affinities, my bias is to use have VIP at the center - eg., type I, type II, type III, type IV VIPR's. THis also takes to account the longer heritage of VIP which was discovered in the early '70s. Ofcourse, if age were the key factor then secretin would have precedence being the first hormone ever to be reported. I don't recall at this instant whether PACAP receptors bind to secretin, and I haven't personally looked into secretin receptors binding PACAP. This will be something to be checked up on. Cheers, Sunil From owner-7tms_r@net.bio.net Thu Apr 20 23:00:00 1995 Path: biosci!CODON.NIH.GOV!usdin From: usdin@CODON.NIH.GOV (Ted Usdin) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 21 Apr 1995 05:26:17 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Is there a consensus on how to refer to the subfamily of receptors which includes the receptors for secretin, VIP, PACAP, glucagon, GLP-I, PTH, calcitonin, CRF...? ****************************** Ted B. Usdin, M.D., Ph.D. Laboratory of Cell Biology, NIMH Bldg 36/Rm3A17 {36 Convent Dr MSC 4090 (U.S. mail)} BETHESDA MD 20892-4090 e-mail usdin@codon.nih.gov tel 301-402-4161 fax 301-402-1748 From owner-7tms_r@net.bio.net Thu Apr 20 23:00:00 1995 Path: biosci!novo.dk!ces From: ces@novo.dk (Carsten E. Stidsen) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: (none) Date: 21 Apr 1995 06:25:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 36 Sender: daemon@net.bio.net Distribution: world Message-ID: <9504211326.AA27387@nngate.novo.dk> NNTP-Posting-Host: net.bio.net >Is there a consensus on how to refer to the subfamily of receptors which >includes the receptors for secretin, VIP, PACAP, glucagon, GLP-I, PTH, >calcitonin, CRF...? > > >****************************** >Ted B. Usdin, M.D., Ph.D. >Laboratory of Cell Biology, NIMH >Bldg 36/Rm3A17 >{36 Convent Dr MSC 4090 (U.S. mail)} >BETHESDA MD 20892-4090 >e-mail usdin@codon.nih.gov >tel 301-402-4161 >fax 301-402-1748 > > > Ted. In "The G-protein linked receptor Facts Book" by Steve Watson and Steve Arkinstall this family is referred to as the "VIP receptor family". The official classification, however, refer to Family B of GPCR=B4s consisting= of three subgroups. Carsten E. Stidsen, Ph.D. Dept. of Molecular Pharmacology Novo Nordisk A/S Novo Alle 1CS.23 DK-2880 Bagsvaerd Phone: +45 4442 2578 Fax: +45 4442 6920 e-mail: ces@novo.dk From owner-7tms_r@net.bio.net Thu Apr 20 23:00:00 1995 Path: biosci!HELIX.NIH.GOV!tibonner From: tibonner@HELIX.NIH.GOV Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Receptor Family Nomenclature Date: 21 Apr 1995 14:16:09 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 58 Sender: daemon@net.bio.net Distribution: world Message-ID: <9504212115.AA22224@helix.nih.gov> NNTP-Posting-Host: net.bio.net >This is an interesting discussion point as there is no consensus on >receptor family nomenclature. > >Kevin Lynch and I have a draft of a manuscript which utilizes the >following nomenclature: > >Family A: The Rhodopsin/beta 2 Adrenergic Receptor Family >Family B: The CT/PTH/Secretin Receptor Family >Family C: The Metabotropic Glutamate/Calcium Sensor Family > >Family D: STE2 fungal pheromone receptor family >Family E: STE3 fungal pheromone receptor family >Family F: cAMP Receptor Family > >GCRDb also uses this nomenclature. > >Publication-wise the Secretin receptor was published first in Family B. >Followed simultaneously by the CT and PTH/PTHrP receptor papers. > >The term the VIP family, I think is way off base despite what the >Watson/Arkinstall book suggests. > > >This is really something that should be discussed especially as the >relationships among these G-protein--coupled receptor families are >unclear at best, and non-existent at worst. > >Frank Kolakowski > Gee, Frank, funny coincidence that GCRDb should use the same nomenclature. Without getting into families C-E which don't have any mammalian members and are thus, I suspect, of less general interest and less in need of a clear family name, I think families A, B, and C or 1,2, and 3 are too cryptic to be useful except among real affectionados. I tend to call them the rhodopsin, secretin and and metabotropic glutamate receptor families naming them after the founding, i.e. first published, member of the family. I think that a set of family name is quite useful in emphasizing that there is little structural similarity between the three families other than the existence of 7 TM domains. Just look at any of the highly conserved amino acids. For example the conserved prolines within the TM domains are in different places in the three families. About the only highly conserved feature in common between families is the the pair of cysteines at the extracellular end of TM3 and between TM4 and TM5, which occurs in both the rhodopsin and secretin receptor families. Tom I. Bonner, Lab of Cell Biology, National Institute of Mental Health Bldg. 36 Room 3A-07, Bethesda MD 20892-4090 Telephone: 301-496-8907, FAX 301-402-1748 tibonner@helix.nih.gov From owner-7tms_r@net.bio.net Thu Apr 20 23:00:00 1995 Path: biosci!ITSA.UCSF.EDU!sredhar From: sredhar@ITSA.UCSF.EDU (Sunil Sreedharan) Newsgroups: bionet.molbio.proteins.7tms_r Subject: secretin receptor and PACAP Date: 21 Apr 1995 16:58:51 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Sunil P. Sreedharan, Ph.D. Assistant Professor, UCSF Div. of Allergy & Immunology Dept. of Medicine, UB8B San Francisco, CA 94143-0711 Tel. (415)476-9895 FAX: (415)476-6915 email: sredhar@itsa.ucsf.edu A correction to my earlier posting, the secretin receptor does bind to PACAP with an affinity similar to that for VIP, i.e., 1000-fold less potent than secretin. Perhaps this subfamily should be called type I - IV secretin receptors after all. Sunil From owner-7tms_r@net.bio.net Sun Apr 23 23:00:00 1995 Path: biosci!ocugw.cc.osaka-cu.ac.jp!h1687 From: h1687@ocugw.cc.osaka-cu.ac.jp (Shimoda) Newsgroups: bionet.molbio.proteins.7tms_r Subject: request Date: 24 Apr 1995 18:32:09 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <9504250133.AA15804@ocugw.cc.osaka-cu.ac.jp> NNTP-Posting-Host: net.bio.net help From owner-7tms_r@net.bio.net Mon Apr 24 23:00:00 1995 Path: biosci!ic.ac.uk!k.hadjantona From: k.hadjantona@ic.ac.uk ("Dr A.K. Hadjantonakis") Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 25 Apr 1995 07:02:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <1767.9504251359@bccsc.bc.ic.ac.uk> NNTP-Posting-Host: net.bio.net Following on from last week's discussion on receptor family nomenclature. I'd like to raise the question of the rather weak homology observed between Family B (secretin R et al) and family F (Dicty cAMP Rs) receptors. The GCRDb paper puts this down to being a fortuitous occurence, is this the general consensus? Is it believed that the observed homologies between these 2 families arose by convergent as opposed to divergent evolution? Kat Hadjantonakis Imperial College of Science, Technology & Medicine, London, U.K. Phone 0171 595 5285 Fax 0171 225 0960 Email kat@ic.ac.uk From owner-7tms_r@net.bio.net Mon Apr 24 23:00:00 1995 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!news.cac.psu.edu!news.pop.psu.edu!psuvax1!news.cc.swarthmore.edu!netnews.upenn.edu!cronkite.ocis.temple.edu!VM.TEMPLE.EDU!SHICKLEY From: SHICKLEY@VM.TEMPLE.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: [2nd Call] Phila. SFN Mtg. May 12 Date: Tue, 25 Apr 95 15:08:46 EDT Organization: Temple University Lines: 81 Message-ID: <1738BD4FES86.SHICKLEY@VM.TEMPLE.EDU> NNTP-Posting-Host: vm.temple.edu <<<<<<<<<<<< MEETING ANNOUNCEMENT AND CALL FOR ABSTRACTS >>>>>>>> ANNUAL MEETING The Philadelphia Chapter of the Society for Neuroscience Friday May 12, 1995 Noon -- 6:00PM Benjamin Franklin Hall American Philosophical Society 427 Chestnut Street Philadelphia, Pennsylvania Preliminary Program (times are approximate): 12:00 - Registration ($10 in advance or at meeting) Exhibits by Corporate Sponsors: Leica Oncor Imaging VWR Scientific Pharmacia Biotech Poster Setup Begin Judging for Eliot Stellar Awards for Neuroscience: Best Undergraduate Research: $100 Best Graduate Research: $100 Best Post-Doctoral Research: $100 3:00 - 2nd Annual Eliot Stellar Awards for Neuroscience 3:30 - Business Meeting, Philadelphia Chapter, Society for Neuroscience. Election of Officers for 1995-1996 3:45 - The Annual Solomon D. Erulkar Memorial Lecture [Sponsored by the Grass Traveling Scientist Program] INTRODUCTION: DR. JOHN O'NEILL Professor Emeritus of Pharmacology Temple University School of Medicine Personal Comments: Mathew Erulkar Lecture: Dr. Eric Kandel Columbia University Title: Genes, Synapses and Long-Term Memory 5:00-6:00 Informal Reception --------------------------------------------------------------------- Abstract Information: In order to encourage interaction of Philadelphia area neuroscientists, we invite participation in the poster session. You may present an abstract from a previous meeting as many from the Philadelphia area did not get to see it at the larger national meeting. We especially encourage students, undergraduate and graduate, and post-doctoral fellows to participate. As a further inducement, we encourage participation in the Eliot Stellar Awards for Neuroscience Research which are $100 prizes in each of the following catagories: Undergraduate research; Graduate research;and Post-doctoral research. Registration for the meeting is $10 payable in advance or at the meeting. Checks should be made out to The Philadelphia Chapter of the Soc. for Neuroscience and sent to the contact person below. Abstracts may be submitted by mail, FAX or by e-mail to the address below. NOTE: Registration for the meeting is required for attending the lecture by Dr. Kandel. Contact Person: Dr. Tim Shickley Dept. of Anatomy and Cell Biology Temple University School of Medicine 3400 North Broad Street Philadelphia, PA 19140 Voice: (215) 707-2920 FAX: (215) 707-2966 shickley@vm.temple.edu _______________________________________________________________________ From owner-7tms_r@net.bio.net Tue Apr 25 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!uhog.mit.edu!rutgers!uwm.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!newsjunkie.ans.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: steved1957@aol.com (SteveD1957) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Rertinoic Acid receptors Date: 25 Apr 1995 23:18:51 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 36 Sender: root@newsbf02.news.aol.com Message-ID: <3nke2r$jqn@newsbf02.news.aol.com> Reply-To: steved1957@aol.com (SteveD1957) NNTP-Posting-Host: newsbf02.mail.aol.com I'm sorry my questions will be vague.... In treating melanoma cells with RA I assume that the goal is to slow/stop CA growth. I'm assuming RA receptors exist on normal cells. I'm not well versed in mol-bio but is the idea here to have RA trigger some sort of cascade response that slows the cancerous growth of the cell? Are G proteins involved in any way? It appears that In Vivo evidence suggests that RA prevents or diminishes the incidence of carcinogen induced mammary tumors. I am interested in the effects of RA on HTB126 mammary fibroblast-like cell line MCF7 mammary epithelial cell line CV-1 green monkey cell :) I'm just starting to dig into books now and my back is against the wall. If you could help me or direct me into the most relevant concept RA would have in relation to these cells I would appreciate it. I'm taking a course where I worked on the above cell lines at Temple Research Center but I have an obstacle in finding relevant information and can't unite my thoughts so as to make a halfway decent paper on the matter. So far most of what I have found has been on the cis-trans aspects, retinol etc. I assume the IRBP (Interstitial Retinol Binding Protein) is involved in the uptake of the RA into the CA cells? Or is this concept totally unrelated? In books I found that there were two RA receptor types???yet isn't there RAR alpha,beta and gamma? Is the trans form of RA the active form that fights CA?? I know I'm asking a lot of you and I'm sorry for that. Again, I would appreciate anything you can get to me or direct me to find Please respond via E-mail Running out of everything fast.... SteveD1957@aol.com From owner-7tms_r@net.bio.net Tue Apr 25 23:00:00 1995 Path: biosci!EMBL-HEIDELBERG.DE!Gert.Vriend From: Gert.Vriend@EMBL-HEIDELBERG.DE Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe Date: 26 Apr 1995 13:25:53 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HPT77NLFO2V7QC2Z@EMBL-Heidelberg.DE> NNTP-Posting-Host: net.bio.net subscribe From owner-7tms_r@net.bio.net Wed Apr 26 23:00:00 1995 Path: biosci!adam.cc.sunysb.edu!news.sprintlink.net!pipex!uunet!noc.near.net!news3.near.net!yale!yale!oitnews.harvard.edu!bloch.nmr.mgh.harvard.edu!nntp.mgh.harvard.edu!lfk From: lfk@receptor.mgh.harvard.edu (Lee F. (Frank) Kolakowski) Newsgroups: bionet.molbio.proteins.7tms_r Subject: 7tms_r Related TOCs for April Date: 27 Apr 1995 11:37:01 GMT Organization: Mass. General Hospital, Renal Unit Lines: 128 Distribution: world Message-ID: NNTP-Posting-Host: receptor.mgh.harvard.edu AU Pfeifer-A. Nurnberg-B. Kamm-S. Uhde-M. Schultz-G. Ruth-P. Hofmann-F. TI Cyclic GMP-dependent protein kinase blocks pertussis toxin-sensitive hormone receptor signaling pathways in Chinese hamster ovary cells SO J-Biol-Chem. 1995 April 21. 270(16). p 9052. AU Ben-Baruch-A. Bengali-K-M. Biragyn-A. Johnston-J-J. Wang-J-M. Kim-J. Chuntharapai-A. Michiel-D-F. Oppenheim-J-J. Kelvin-D-J. TI Interleukin-8 receptor {beta}. The role of the carboxyl terminus in signal transduction. SO J-Biol-Chem. 1995 April 21. 270(16). p 9121. AU Watson-A. Latchman-D. TI The cyclic AMP response element in the calcitonin/calcitonin gene-related peptide gene promoter is necessary but not sufficient for its activation by nerve growth factor SO J-Biol-Chem. 1995 April 21. 270(16). p 9655. AU Wilson-P-T. Bourne-H-R. TI Fatty acylation of {alpha}z. Effects of palmitoylation and myristoylation on {alpha}z signaling. SO J-Biol-Chem. 1995 April 21. 270(16). p 9667. AU Parker-E-M. Swigart-P. Nunnally-M-H. Perkins-J-P. Ross-E-M. TI Carboxyl-terminal domains in the avian {beta}1-adrenergic receptor that regulate agonist-promoted endocytosis. Vol. 270 (1995) 6482-6487. SO J-Biol-Chem. 1995 April 28. 270(17). p 10358. AU Hunyady-L. Bor-M. Balla-T. Catt-K-J. TI Critical role of a conserved intramembrane tyrosine residue in angiotensin II receptor activation SO J-Biol-Chem. 1995 April 28. 270(17). p 9702. AU Wu-D. Jiang-H. Simon-M-I. TI Different {alpha}1-adrenergic receptor sequences required for activating different G{alpha} subunits of Gq class of G proteins SO J-Biol-Chem. 1995 April 28. 270(17). p 9828. AU Takagi-Y. Ninomiya-H. Sakamoto-A. Miwa-S. Masaki-T. TI Structural basis of G protein specificity of human endothelin receptors. A study with endothelinA/B chimeras. SO J-Biol-Chem. 1995 April 28. 270(17). p 10072. AU Valgeirsdottir-S. Eriksson-A. Nister-M. Heldin-C-H. Westermark-B. Claesson-Welsh-L. TI Compartmentalization of autocrine signal transduction pathways in Sis-transformed NIH 3T3 cells SO J-Biol-Chem. 1995 April 28. 270(17). p 10161. AU Goodemote-K-A. Mattie-M-E. Berger-A. Spiegel-S. TI Involvement of a pertussis toxin-sensitive G protein in the mitogenic signaling pathways of sphingosine 1-phosphate SO J-Biol-Chem. 1995 April 28. 270(17). p 10272. AU Schraufstatter-I-U. Ma-M. Oades-Z-G. Barritt-D-S. Cochrane-C-G. TI The role of Tyr13 and Lys15 of interleukin-8 in the high affinity interaction with the interleukin-8 receptor type A SO J-Biol-Chem. 1995 May 5. 270(18). p 10428. AU Ernst-O-P. Hofmann-K-P. Sakmar-T-P. TI Characterization of rhodopsin mutants that bind transducin but fail to induce GTP nucleotide uptake. Classification of mutant pigments by fluorescence, nucleotide release, and flash-induced light-scattering assays. SO J-Biol-Chem. 1995 May 5. 270(18). p 10580. AU Prossnitz-E-R. Schreiber-R-E. Bokoch-G-M. Ye-R-D. TI Binding of low affinity N-formyl peptide receptors to G protein. Characterization of a novel inactive receptor intermediate. SO J-Biol-Chem. 1995 May 5. 270(18). p 10686. AU Rohlfs-E-M. Daniel-K-W. Premont-R-T. Kozak-L-P. Collins-S. TI Regulation of the uncoupling protein gene (Ucp) by {beta}1, {beta}2, and {beta}3-adrenergic receptor subtypes in immortalized brown adipose cell lines SO J-Biol-Chem. 1995 May 5. 270(18). p 10723. AU Endo-T. Ohta-K. Haraguchi-K. Onaya-T. TI Cloning and functional expression of a thyrotropin receptor cDNA from rat fat cells SO J-Biol-Chem. 1995 May 5. 270(18). p 10833. AU Chun-M. Lin-H-Y. Henis-Y-I. Lodish-H-F. TI Endothelin-induced endocytosis of cell surface ETA receptors. Endothelin remains intact and bound to the ETA receptor. SO J-Biol-Chem. 1995 May 5. 270(18). p 10855. AU Feve-B. Pietri-Rouxel-F. Hadri-K-E. Drumare-M-F. Strosberg-A-D. TI Long term phorbol ester treatment down-regulates the {beta}3-adrenergic receptor in 3T3-F442A adipocytes SO J-Biol-Chem. 1995 May 5. 270(18). p 10952. AU Mhaouty-S. Cohen-Tannoudji-J. Bouet-Alard-R. Limon-Boulez-I. Maltier-J-P. Legrand-C. TI Characteristics of the {alpha}2/{beta}2-adrenergic receptor-coupled adenylyl cyclase system in rat myometrium during pregnancy SO J-Biol-Chem. 1995 May 5. 270(18). p 11012. AU Rivard-N. McKenzie-F-R. Brondello-J-M. Pouyssegur-J. TI The phosphotyrosine phosphatase PTP1D, but not PTP1C, is an essential mediator of fibroblast proliferation induced by tyrosine kinase and G protein-coupled receptors SO J-Biol-Chem. 1995 May 5. 270(18). p 11017. AU R-De Cristofaro. E-De Candia. M-Picozzi. R-Landolfi TI Conformational Transitions Linked to Active Site Ligation in Human Thrombin: Effect on the Interaction with Fibrinogen and the Cleavable Platelet Receptor SO Journal of Molecular Biology, 1995 January. 245 (4) pp. 447-458 AU Milligan-G. Parenti-M. Magee-A-I. TI The dynamic role of palmitoylation in signal transduction. DE palmitoylation DE receptor DE tyrosine kinase DE G protein DE acylation SO Trends-Biochem-Sci. 1995 May. 20(5). P. 181-186. CC Review -- Frank Kolakowski Email: lfk@receptor.mgh.harvard.edu 617-355-7515 (LAB) GCRDb-WWW From owner-7tms_r@net.bio.net Thu Apr 27 23:00:00 1995 Path: biosci!SAPMED.AC.JP!sohma From: sohma@SAPMED.AC.JP (Hitoshi Sohma) Newsgroups: bionet.molbio.proteins.7tms_r Subject: help Date: 28 Apr 1995 01:38:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <9504280840.AA15507@serpent.cc.sapmed.ac.jp> NNTP-Posting-Host: net.bio.net help From owner-7tms_r@net.bio.net Sun Apr 30 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!news.starnet.net!wupost!uwm.edu!cs.utexas.edu!howland.reston.ans.net!pipex!lyra.csx.cam.ac.uk!mole.bio.cam.ac.uk!flh From: flh@mole.bio.cam.ac.uk (Frances Hannan (Zoology)) Newsgroups: bionet.molbio.proteins.7tms_r Subject: pM ligands (summary of replies) Date: 1 May 1995 15:35:16 GMT Organization: University of Cambridge, England Lines: 54 Message-ID: <3o2v3k$6li@lyra.csx.cam.ac.uk> NNTP-Posting-Host: mole.bio.cam.ac.uk To: bionet.molbio.proteins.7tms_r Subject: pM ligands (summary) Thanks to all those people who replied to my request for information on ligands which activate their receptor in the pM range. Here is a summary of the replies I received: From: thatsu There is significant activation of the thrombin receptor by thrombin in Xenopus oocytes overexpressing mammalian receptors. From: Patrick M Sexton CT can act in the pM range at the osteoclast, both in terms of cAMP production and functionally in inhibiting osteoclast function. However, in other systems eg. cells expressing endogenous CT receptor such as T47D, UMR 106-06 or cells transfected with cloned receptors we tend to see less sensitive responses eg. thresholds around 10-11 M. From: PETER N MONK We have shown that human C5a (from complement component C5) can activate a weak calcium influx in differentiated U937 cells at 50pM (see BJ 295:679, 1993). We also have some evidence that these cells can be primed for a later response by pretreatment with C5a; maximal priming occurred at 5pM (unpublished). The Kd of the receptor on these cells is approx. 1nM, and C5a has a range of effects on these cells up to 20nM. Only one form of C5a receptor mRNA can be found in these cells, so the mechanism for this large concentration range is unclear. From: "T. J. Murphy" This may not be what you're looking for, but I once got some pretty potent responses for compounds activating the opossum 5HT1b receptor (JPET 249:535-543, 1989). An ergotamine derivative had potency approaching pM. Since its not the "natural" agonist, this might not be what you're after. You also might want to search Judy Cole in the late 1980's early 1990's for effects of PTH on phosphate transport in renal proximal tubular cells. I recall she was getting some responses in the (sub?) picomolar range. -- Frances Hannan BILMS, Zoology, Downing St, Cambridge, CB2 3EJ, UK Phone (0223)336663, FAX (0223)461954 flh@mole.bio.cam.ac.uk From owner-7tms_r@net.bio.net Sun Apr 30 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!news.starnet.net!wupost!howland.reston.ans.net!swrinde!news.uh.edu!uuneo.neosoft.com!scooter-slip-b5 From: shark@sam.neosoft.com (John D. Valentich) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Apical membrane receptor regulation of epithelial cells Date: 2 May 1995 02:44:47 GMT Organization: University of Texas Medical Branch Lines: 31 Message-ID: <3o46av$sb1@uuneo.neosoft.com> NNTP-Posting-Host: scooter-slip-b5.neosoft.com X-Newsreader: News Xpress Version 1.0 Beta #3 Traditionally, epithelia are thought to be controlled by endocrine and neurocrine mediators interacting with BASOLATERAL plasma membrane receptors. Clearly, there is another side to this story. We are writing a review article with the tentative title, "Epithelial Functions Regulated by Apical Membrane Receptors". Our working table lists over 30 published examples of physiological responses in epithelia following addition of mediators to the apical plasma membrane domain. Major organ systems, such as the airways, intestines, excretory systems, and male and female reproductive systems are represented. In spite of this emerging literature, a wide appreciation of this phenomenon is lacking. The key concept is that apical receptors participate in the local control of epithelial function. We want to compile a complete list of examples in which this regulatory scheme has been described or suggested. If you have observed this phenomenon or have thoughts about its physiological meaning we would welcome your comments and any information about your publications, in print or in press. Please write either: Karl Karnaky (KKARNAKY@LIFE.JSC.NASA.GOV) or John Valentich (SHARK@SAM.NEOSOFT.COM) Thanks