From owner-7tms_r@net.bio.net Tue Apr 02 23:00:00 1996 Path: biosci!bloom-beacon.mit.edu!panix!netaxs.com!news2.cais.net!news.cais.net!chi-news.cic.net!news.compuserve.com!news.production.compuserve.com!news From: Greg LaRosa <72541.220@CompuServe.COM> Newsgroups: bionet.molbio.proteins,bionet.molbio.proteins.7tms_r Subject: GPCR Expression and Membrane Preps Date: 4 Apr 1996 05:29:06 GMT Organization: LeukoSite, Inc. Lines: 15 Message-ID: <4jvmn3$3d4$1@mhafn.production.compuserve.com> Xref: biosci bionet.molbio.proteins:7507 bionet.molbio.proteins.7tms_r:688 Anyone have any experience with improving poor surface expression of cloned GPCR in transfected cells? Also any ideas for making membrane preps for radioligand binding assays. I have been working with a receptor for which I have not been able to membrane preps that retain the binding activity of the intact cells; eventhough the same membrane isolation procedure (hypotonic lysis in hte presence of protease inhibitors, followed by a low speed cent.to remove unlysed cells and large debris, and then membrane harvesting by high speed cent.) has worked well for a number of other GPCR in my hands. Any thoughts appreciated -- Greg LaRosa From owner-7tms_r@net.bio.net Sun Apr 07 23:00:00 1996 Path: biosci!MIRIS.MED.YALE.EDU!mike From: mike@MIRIS.MED.YALE.EDU ("Michael Singer") Newsgroups: bionet.molbio.proteins.7tms_r Subject: OLFACTORY RECEPTOR DATABASE Date: 8 Apr 1996 16:58:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 31 Sender: daemon@net.bio.net Distribution: world Message-ID: <9604081955.ZM19926@miris.med.yale.edu> NNTP-Posting-Host: net.bio.net The Olfactory Receptor DataBase (ORDB), is now available on the World-Wide-Web: http://senselab.med.yale.edu/ordb Public access is available to all. Laboratories which clone olfactory receptors should contact Michael Singer [mike@miris.med.yale.edu] for additional access. Michael S. Singer Section of Neurobiology Jason E. Smith Gordon M. Shepherd Matthew D. Healy Center for Medical Informatics Prakash M. Nadkarni Perry L. Miller Yale University School of Medicine 333 Cedar Street New Haven, CT 06510 USA The ORDB is supported by the Human Brain Project (NIMH, NASA, & NIDCD) Grant R01 DC02307 and by NIH Grant G08LM05583 from the National Library of Medicine's IAMS Program. From owner-7tms_r@net.bio.net Mon Apr 08 23:00:00 1996 Path: biosci!PICAN.PI.CSIRO.AU!masumi From: masumi@PICAN.PI.CSIRO.AU ("Masumi Robertson ", X) Newsgroups: bionet.molbio.proteins.7tms_r Subject: dose response Date: 9 Apr 1996 16:24:22 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear netters, Are there any examples where higher concentration of ligand required to elicit a given response is NOT due to mutation in the receptor (if the mutation is 'leaky' and 'sensitivity is altered')? Would a shift in a dose-response curve be due to defects in something (signal components?) other than the receptor? Thanks for your comments! Masumi Robertson phone: +61 (6) 246-5030 Division of Plant Industry, CSIRO fax: +61 (6) 246-5000 GPO Box 1600 Canberra, ACT 2601 Australia From owner-7tms_r@net.bio.net Tue Apr 09 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!newsfeed.sunet.se!news00.sunet.se!sunic!mn6.swip.net!seunet!news2.swip.net!inet-1.pharmacia.se!usenet From: Poul Sorensen Newsgroups: bionet.molbio.proteins.7tms_r Subject: human c-rel & p65 Date: 10 Apr 1996 14:21:21 GMT Organization: Pharmacia & Upjohn Oncology Immunology, Lund, Sweden. Lines: 4 Message-ID: <4kgg51$l46@inet-1.pharmacia.se> NNTP-Posting-Host: pcsopo.lun.se.pnu.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Does anyone know if human c-rel and p65 are commmercially available ? Poul Sorensen. From owner-7tms_r@net.bio.net Thu Apr 11 23:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.proteins.7tms_r Subject: IMPORTANT - BIOSCI Fundraising Update! Date: 12 Apr 1996 02:00:32 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 149 Sender: daemon@net.bio.net Distribution: world Message-ID: <199604120900.CAA01310@net.bio.net> NNTP-Posting-Host: net.bio.net I'm interrupting the usual monthly posting of the BIOSCI miniFAQ to bring you up to date on BIOSCI fundraising progress, a topic of concern to your future use of this resource. Thank you in advance for taking the time to read this message carefully. Last year we announced that BIOSCI was going to adopt the U.S. Public Broadcasting System model to fund its operations after our DOE/NSF grant runs out later this year. Unlike PBS, we are not soliciting contributions from users; we are only selling ads on our Web pages solely to cover our operating costs. Our goal is to seek sponsorships until we build up an operating reserve of about $100,000 and then cease further promotions until we need to build the reserve back up. (The accountants among our readership will be familiar with the problem of deferred revenue which we can not safely utilize until ads have been displayed for a period of time.) We have three sponsors to date with a couple more pending. The process is time-consuming, however, and we need your help as explained further below. Our operating costs consist of our network connection, phone lines, hardware maintenance (we hope to have new and faster hardware soon!), plus 0.7 FTE of salaries covering UNIX systems admin, technical support, quality assurance, i.e., testing, of our system, and administrative costs (such as the time it takes to actually find/write/call potential sponsors and raise money!). Although the BIOSCI staff does get compensated for a portion of the work that they do, this project has always received a lot of free after-hours and "vacation" time labor, so we hope that no one will begrudge the time that we do charge to the project to serve you. All of the three part-time staff members, Dave Mack, Julie Lawrence, and myself, have full time day jobs and families in addition to working hard to keep this service running for all of you. Julie and Dave Mack are subcontractors for BIOSCI; my time that is charged to the project defrays a portion of my regular salary instead of adding to my income. Besides having to relocate the project, we were very busy this last year building new infrastructure such as our WWW hypermail interface to the system. This was released last December along with scores of WAIS indices for the newsgroups. Virtually everything is complete, although we do continue to find and fix bugs (many through your helpful feedback!). We are still having some problems with our WAIS indexing. The archives continue to grow rapidly. We are running over 100 indexes now versus three previously and any systems crashes cause greater havoc with the indexing than before! We are still working to fix this as fast as our resources permit and appreciate your patience, but we have been able to automate a lot of the infrastructure to reduce labor as compared to past requirements. We have also implemented new software to make moderation of BIOSCI/bionet newsgroups much easier and combat the growing problem of Internet junk mail and USENET "spamming." About 20% of our groups are now moderated, many of them by the BIOSCI staff! This, for example, made a major difference last year in the quality of content in our EMPLOYMENT/bionet.jobs.offered newsgroup which many commercial concerns and recruiting firms are using **without charge** to recruit candidates for positions in the biological sciences. We are also now in a position to have sponsors for individual newsgroups as you will have noticed if you have visited http://www.bio.net/ and clicked on "Access the BIOSCI/bionet newsgroups" recently. So, how can you help?? ---------------------- As noted above it can take a lot of time to contact potential sponsors if I have to do it all myself. Our request is quite simple. You can do two important things which will take very little time for you individually. First, please use our WWW system at http://www.bio.net/ to access the archives. You can now post or reply to messages via your Web browser. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. Our hope is to quickly raise several large corporate/institutional sponsors on our heavily-used WWW locations (some stats appended below), and then end this sponsorship campaign so that our resources can continue to be used for service provision, not fundraising. Many of our specialty newsgroup WWW archives are still used by small communities of scientists (and they haven't been heavily promoted yet). While these may be valuable niche markets to some advertisers, it will generate more labor and overhead having to find these sponsors, fairly price the locations, and deal with lots of smaller sponsorships than fewer mid-to large sponsors. We are striving to keep our operation as lean and efficient as possible since we are not trying to make careers out of running BIOSCI. We are trying if at all possible to avoid the administrative overhead entailed with processing lots of small payments to reach our fundraising goals. I'd like to thank all of you for your help in advance. In helping us, you are also helping yourselves, not only in keeping this resource available for all of the both large and small research communities that we serve, but also by alleviating the need for us to go back and compete with researchers for tight grant dollars! We promised NSF when we were awarded the BIOSCI grant that we would carry out this mission to make the service self-supporting. With your help, we will succeed in continuing BIOSCI's work into its second decade. Thank you very much! Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net A list of our prime WWW sponsorship locations follow. Statistics are for the four week period from 22 Jan. - 18 Feb. 1996 and usage continues to grow. ---------------------------------------------------------------------- The overall BIOSCI WWW pages are currently visited by users from close to 5000 unique computer hosts per week. Web servers only log the Internet computer/host name and frequently more than one individual can connect to us from a particular host. Main home page, http://www.bio.net, visited recently by about 2100 unique hosts per week Main Newsgroups archives page, http://www.bio.net/archives.html, visited recently by about 1200 Unique hosts per week BIO-JOURNALS archive page, http://www.bio.net/BIO-JOURNALS.html, visited recently by about 1000 unique hosts per week. EMPLOYMENT archive pages: http://www.bio.net:80/hypermail/EMPLOYMENT/ and monthly header pages, visited recently by about 600 unique hosts per week. Address database search page, http://www.bio.net/addrsearch.html, visited recently by about 450 unique hosts per week. Methods newsgroup archive pages, http://www.bio.net:80/hypermail/METHDS- REAGNTS/ and monthly header pages, visited recently by about 350 unique hosts per week. ---------------------------------------------------------------------- From owner-7tms_r@net.bio.net Thu Apr 11 23:00:00 1996 Path: biosci!galaxy.ucr.edu!library.ucla.edu!csulb.edu!drivel.ics.uci.edu!news.service.uci.edu!unogate!news.intelenet.com!news.sprintlink.net!new-news.sprintlink.net!newsreader.sprintlink.net!imci3!imci4!newsfeed.internetmci.com!tank.news.pipex.net!pipex!oleane!in2p3.fr!swidir.switch.ch!scsing.switch.ch!news.belwue.de!news.uni-ulm.de!auvi-mac.informatik.uni-ulm.de!user From: wolf@informatik.uni-ulm.de (Heiner Wolf) Newsgroups: bionet.microbiology,bionet.molbio.proteins,bionet.molbio.proteins.7tms_r,bionet.xtallography,sci.bio.microbiology Subject: Replacement for E.col at pH 3 ? Date: Fri, 12 Apr 1996 17:11:24 +0200 Organization: Distributed Systems, University Ulm Lines: 12 Message-ID: NNTP-Posting-Host: power_mac.informatik.uni-ulm.de Xref: biosci bionet.microbiology:5695 bionet.molbio.proteins:7555 bionet.molbio.proteins.7tms_r:693 bionet.xtallography:2493 sci.bio.microbiology:3144 My protein is stable at pH strength 3. But E. coli is inactive there. Which organism can replace E. coli at pH 3 ? Please send email to wolf@informatik.uni-ulm.de -Heiner Wolf -- Distributed Systems Dept. voice: +49 731 502 4145 University of Ulm internet: wolf@informatik.uni-ulm.de 89069 Ulm ethernet: 08:00:20:12:2a:01 Germany WebVideo: http://rr-vs.informatik.uni-ulm.de/rr/ From owner-7tms_r@net.bio.net Thu Apr 11 23:00:00 1996 Path: biosci!agate!howland.reston.ans.net!swrinde!newsfeed.internetmci.com!uwm.edu!lepidopteran.cse.psu.edu!news.cc.swarthmore.edu!netnews.upenn.edu!cronkite.ocis.temple.edu!VM.TEMPLE.EDU!SHICKLEY From: SHICKLEY@VM.TEMPLE.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: CFA:Annual Mtg. Phila. Chapt. SFN 5/3/96 Date: Fri, 12 Apr 96 10:06:41 EDT Organization: Temple University Lines: 233 Message-ID: <177678E31S86.SHICKLEY@VM.TEMPLE.EDU> NNTP-Posting-Host: vm.temple.edu Electronic Version of Synapse, Volume XX, Number 3. April 8, 1996. Newsletter of the Philadelphia Chapter of the Society for Neuroscience Editor: Mary Barbe. (mbarbe@aol.com) In this Issue: CALL FOR ABSTRACTS: Chapter Annual Meeting Plans Chapter Booth at Neurochemistry Report Tom Rainbow Lecture Report Dues Notice Current Coucil Members. Upcoming Seminars & Courses Thanks to our Corporate Members Please attend the annual meeting May 3rd in Philadelphia! 1. Calendar for remaining year: Abstracts for Chapter Ann. Mtg. due April 22 or 29 depending on if you want to present via platform or poster format.(Request abstract form from Doug Baird, Chapter Secretary: baird@medcolpa.edu) email your abstract (350 words +/-) to Doug Baird at the same email address. Fax your abstract to Doug at (215)843-9082. Undergraduate, Graduate students and Postdoctoral fellows are eligible for the Eliot Stellar Awards for Neuroscience Research. The award is $100 for each catagory. Indicate on the abstract form that you wish to compete for the awards. 2. Annual Meeting Plans. Chapter Annual Meeting: May 3,1996 10:00AM - 6:00PM Benjamin Franklin Hall of the American Philosophical Society 427 Chestnut Street, Philadelphia, PA Sol Erulkar Memorial Lecture: Phillip G. Nelson, MD PhD Dir. Lab. of Developmental Neurobiology NIH-NICHD "Experiential Shaping of the Developing Brain: Mechanisms of Synapse Loss" Many additional poster boards are being arranged for use this year; we would also like to give students the opportunity to give platform talks. See abstract form on page 9 for deadlines and form. We are expanding the program to begin at 10 am and extend to 6 pm to allow for more time for interactions at the posters and exhibits. The day will also include platform talks by Chapter members, a seminar by Phillip Nelson, as well as a Wine and Cheese reception following all. 3. Chapter Booth at the Neurochemistry Society Meeting this March: The booth featured an excellent poster put together by Valentino "Butch" Piacentino III and Bill Saidel, from Rutgers, Camden. The poster included representative work by various Chapter members. January newsletters and abstract forms for the Annual meeting were available as handouts. 4. The Thomas Rainbow Memorial Lecture was held on March 22. This lecture is an annual event sponsored by the Philadelphia Chapter of SFN and the Department of Pharmacology of the University of Pennsylvania. This year's lecture, held at the University of Pennsylvania's Faculty Club, was presented by Dr. Donald Pfaff of the Rockefeller University. His talk, entitled "Genes and Behavior as Managed by Hormone-Dependent Hypothalamic Neurons" was introduced by Dr. Trevor Penning, Acting Chair of the Dept. of Pharmacology following welcoming remarks by Dr. Tim Shickley of Temple University, the Presiden of the Philadelphia Chapter of SFN. Dr. Pfaff's lecture was a tour de force presentation linking mating behavior with molecular events and endocrine function. All attending had an ample opportunity to visit with Dr. Pfaff and to mix with friends and colleagues at the wine and cheese social following the lecture. This was just one event which your dues go to sponsor. 5. Have You Paid Your Dues for This Year? Membership dues in the Philadelphia Area Chapter of Neuroscience are only $10 per year (students are free if their advisors are also a member). These dues help defray the cost of publication and postage on the newsletter, help pay honoraria for guest speakers, pay for A/V services and refreshments for our meetings. Your dues help, as we have no other sources of revenue. If you haven't done so, please send your check to the treasurer: Russ Buono Department of Pathology, Anatomy and Developmental Biology Thomas Jefferson University 1020 Locust Street Phil., PA 19107 (215) 955-7852 6. Chapter's Council Members : President: Tim Shickley, Temple University. Tel: 215/707-2922, email: shickley@vm.temple.edu Vice President: Mary Barbe, Temple University, Tel: 215/707-4896, email: mbarbe@aol.com Secretary: Doug Baird, Medical College of PA and Hahnemann, Tel: 215/842-4606, email: baird@medcolpa.edu Treasurer: Russ Buono, Thomas Jefferson University,Tel: 215/955-7852, email: rbuono@tjuvm.tju.edu Past President: Helen Pearson, Temple University,pearson@vm.temple.edu Councilors: Nancy Bonini of Univ. of Pennsylvania, email: bonini@sas.upenn.edu Don Faber of Medical College of PA and Hahnemann. faber@medcolpa.edu Amita Sehgal of U Penn. amita@mail.med.upenn.edu Bill Saidel of Rutgers University.Saidel@crab.rutgers.edu Steve Wieland of Medical College of PA and Hahnemann, wielands@hal.hahnemann.edu Rob Waldeck of Medical College of PA and Hahnemann, waldeck@medcolpa.edu 7. Current email addresses are needed for all Chapter members. Please send email addresses of any members you know or anyone you think might want to be a member to mbarbe@aol.com. 8. Seminar Notices : Some additional seminars have been added since the Jan. Synapse-- April 9 and April 10 at Univ. of Delaware. Please note here for additional information concerning seminars listed. DAVID MAHONEY INSTITUTE OF NEUROLOGICAL SCIENCES, UNIVERSITY OF PENNSYLVANIA. Neuroscience Conference Room/Library, 140 John Morgan Building / 36th & Hamilton Walk, Refreshments at 4:00 / Colloquia begin at 4:15. For additional information, call: 215/898-8754. MEDICAL COLLEGE OF PENNSYLVANIA AND HAHNEMANN UNIVERSITY, Dept of Pharmacology. Please contact David Snyder at 842-4765 for further information. MEDICAL COLLEGE OF PENNSYLVANIA AND HAHNEMANN UNIVERSITY,Center for Neurobiology, Dept of Neurobiology and Anatomy. Michael E. Goldberger Conference Room, EPPI Building, 9th floor, Room 960. Please contact Kathy Elliot (215 842-4471) for more information. RUTGERS UNIVERSITY at Newark, NJ, Center for Molecular and Behavioral Neuroscience. Thursdays 4:30 pm. Neurosci Seminar Room, Aidekman Research Center. 197 Univ Ave, Newark, NJ 17102. Call Ralph Seigal 201-648-1080 X 3294. TEMPLE UNIVERSITY, SCHOOL OF MEDICINE, Dept of Psychiatry Grand Rounds. Hospital Auditorium. 3401 N. Broad Street. Fridays, 12:00-1:15 pm. UNIVERSITY OF DELAWARE, Newark, DE. Please contact Jerome Siegel at 302/831-5492. UNIVERSITY OF PENNSYLVANIA, Dept of Pharmacology. Pharmacology Seminar Room M100-101, Mezzanine. John Morgan Building. Call 215/898-8416. March: March 29. Syed Zaidi. Suicide. Dept of Psychiatry. Temple Univ. Temple Univ, Dept of Psychiatry Grand Rounds. 12:00 -1:15 pm April: April 3. Martha Gillette. The Neurobiology of time; regulation of the brain's biological clock. Dept of Cell and Strutural Biology, U Illinois, MCP and Hahnemann, Dept of Neurobiology and Anatomy. 4 pm. April 3. Peter Strick. Basal ganglia and cerebellar loops with the cerebral cortex: new concepts about their structure and function. Depts of Neurosurgery and Physiology, State Univ of NY Health Science Center in Syracuse, David Mahoney Inst. U Penn, 4 pm. April 8. Hugh Hemmings. Dept of Anesthesiology, Cornell Univ Medical School. MCP and Hahnemann, Dept of Pharmacology. April 9. Rex Wang. The mode of acion of hallucinogens: The role of 5HT2A receptors in the medial prefrontal cortex. Dept of Psychiatry and Behav. Science, SUNY Stoney Brook. Dept. of Psychology , Univ. of Delaware. 4 pm. April 10. Barbara Jones. Cholinergic neurons in the brain: Their role in cortical activation and sleep-wake states. Montreal Neurological Institute, McGill Univ. Department of Psychology, Univ. of Delaware. 4 pm. April 10. Josef Rauschecker. Compensatory plasticity and sensory substitution in the cerebral cortex. Lab. of Neuropsychology, Section of Cognitive Neuroscience, NIMH. David Mahoney Inst, U Penn, 4 pm. April 12. William Cross. Racial identity formation. Dept of Psychiatry. Penn State Univ. Temple Univ, Dept of Psychiatry Grand Rounds. 12:00-1:15 pm April 17. Harry Orr. Univ of Minnesota, Institute of Human Genetics. MCP and Hahnemann, Dept of Neurobiology and Anatomy. 4 pm. April 18. Huda Akil.Endogenous opioid receptors and their relation to endogenous ligands. Mental Health Research Institute University of Michigan. RUTGERS UNIVERSITY at Newark, NJ, Center for Molecular and Behavioral Neuroscience. Thursdays 4:30 pm. April 19. Joan McCord. Crime prevention treatment effect: A cautionary tale. Professor of Criminal Justice. Temple Univ. Temple Univ, Dept of Psychiatry Grand Rounds. 12:00 pm -1:15 pm April 26. Steven King. What's new in pain management. Dept of Psychiatry. Temple Univ. Temple Univ, Dept of Psychiatry Grand Rounds. 12:00-1:15 pm April 29. Christopher Kramer. Dept of Medicine. Allegheny General Hospital. MCP and Hahnemann, Dept of Pharmacology. May: May 1. Virginia Brooks. Angiotensin II, the sympathetic nervous system and long-term control of arterial blood pressure. Dept of Physiology, Oregon Health Science Univ. David Mahoney Inst, U Penn, 4 pm. May 3. Raquel Gur. Studies of brain and behavior in schizophrenia. Dept of Psychiatry, U Penn. Temple Univ, Dept of Psychiatry Grand Rounds. 12:00-1:15 pm May 8. Cathy Wedeen. Expression of segment-polarity gene homologs in the leech. Dept of Cell Biol and Anatomy. New York Medical College. David Mahoney Inst, U Penn, 4 pm. May 17. Anthony Rothchild. The diagnosis, biology and treatment of psychiatric depression. Dept of Psychiatry, McLean Hospital. Temple Univ, Dept of Psychiatry Grand Rounds. 12:00-1:15 pm May 20. Maria Erecinska. Dept of Pharmacology, U Penn. MCP and Hahnemann, Dept of Pharmacology. May 30. V.S. Ramachandran. Illusions of body image: what they reveal about human nature. Dept of Psychology University of California, San Diego. RUTGERS UNIVERSITY at Newark, NJ, Center for Molecular and Behavioral Neuroscience. Thursdays 4:30 pm. May 31. John Siberski. Role of consultation in evaluation. Consultation/Liaison Director. Temple Univ, Dept of Psychiatry Grand Rounds. 12:00-1:15 pm Courses. Pathophysiology of secondary brain Injury and implications for contemporary Treatment. Course Director: Donald W. Marion, MD. May 17-18, 1996. Sheraton Hotel at Station Square Pittsburgh, PA. University of Pittsburgh Medical Center. The Brain Trauma Research Center. For more information call: Carol Wistzki at (412)647-9542 or fax her at (412) 647-8222. 9. CORPORATE MEMBERS: We would like to offer special thanks to our corporate members for their support during 1995-1996. Pharmacia Biotech VWR Scientific Oncor Imaging From owner-7tms_r@net.bio.net Sat Apr 13 23:00:00 1996 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!news.compuserve.com!news.production.compuserve.com!news From: ROBYN ONEAL <102170.125@CompuServe.COM> Newsgroups: bionet.molbio.proteins,bionet.molbio.proteins.7tms_r,sci.bio.misc,sci.bio.technology,sci.chem Subject: ELECTROPORESIS,PROTEIN GELS Date: 15 Apr 1996 01:19:05 GMT Organization: CompuServe, Inc. (1-800-689-0736) Lines: 8 Distribution: inet Message-ID: <4ks869$pk1$1@mhadg.production.compuserve.com> Xref: biosci bionet.molbio.proteins:7570 bionet.molbio.proteins.7tms_r:695 sci.bio.misc:2863 sci.bio.technology:5203 sci.chem:53931 IF YOU WORK IN A LAB THAT USES ACRYLAMIDE GELS. I HAVE FOUND A NEW PRODUCT THAT SAVED ME TIME IN THE PREPARATION TO RUN A GEL. I FOUND THE GEL EASY TO WORK WITH AND THE BANDS TO COME OUT WELL FORMED AND CLEAR. I FOUND INFORMATION ABOUT THIS NEW TYPE OF GEL ON THE WEB. URL ADDRESS HTTP://HOME.NAVISOFT.COM/PROFBUS/ESSEX1.HTM ROBYN O'NEAL From owner-7tms_r@net.bio.net Mon Apr 15 23:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!newsxfer2.itd.umich.edu!newsxfer.itd.umich.edu!news.join.ad.jp!news.imnet.ad.jp!lab!wsclark!nns!usenet From: kazuhsi urasawa Newsgroups: bionet.molbio.proteins.7tms_r Subject: GRK5 Date: Tue, 16 Apr 1996 19:57:37 +0900 Organization: School of Medicine, Department of Cardiovascular Medicine Lines: 3 Message-ID: <31737D21.3DA@med.hokudai.ac.jp> NNTP-Posting-Host: kurasawa.med.hokudai.ac.jp Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01Gold (Win95; I) Is there any pathological conditions where the expression of GRK5 is altered ? Please e-mail me if somebody has information on this issue. From owner-7tms_r@net.bio.net Wed Apr 17 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.swt.edu!newsmaster From: Ari Kahn Newsgroups: bionet.molbio.proteins.7tms_r Subject: GFAP immunofluoresence of c6 cell line Date: Fri, 19 Apr 1996 00:49:27 +0000 Organization: Southwest Texas State University Lines: 12 Message-ID: <3176E317.7F0C@swt.edu> NNTP-Posting-Host: port20.modem1.cc.swt.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; PPC) I am doing some immunochemistry on a C6 rat glioma cell line. I immunostained cells on a coverslip with GFAP primary antibody. Microscopy of these C6 cells showed fluoresence, a positive result, no. However, I did a Western Blot of these cells and the results for GFAP were negative. I have heard that C6 cells do not express GFAP. Is this true and if so can anyone recomend a paper discussing this? Also, since my Western was negative, what was fluoresing on my cells? Thank you for any input, Ari Kahn ak01706@swt.edu From owner-7tms_r@net.bio.net Wed Apr 17 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!howland.reston.ans.net!surfnet.nl!swsbe6.switch.ch!news.belnet.be!news.sri.ucl.ac.be!jussieu.fr!citi2.fr!camoin.cochin.inserm.fr!user From: brydon@icgm.cochin.inserm.fr (lena brydon) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Review GPCR-Antibody Production Date: Thu, 18 Apr 1996 17:29:09 +0200 Organization: icgm cnrs upr415 Lines: 17 Message-ID: NNTP-Posting-Host: camoin.cochin.inserm.fr X-Newsreader: Yet Another NewsWatcher F2.0 Dear Netters, I plan to compile a review of 1. existing methods or novel ideas for raising specific monoclonal and polyclonal antibodies against G-protein-coupled receptors and 2. the potential uses of such antibodies. I would be very grateful for any information/ideas you have regarding this subject area. The results of this inquiry will be sent to this news group later. If anyone has any papers recently published or in Press regarding this subject and are willing to send me re-prints, I will of course quote these authors in the review ( if so desired.) Lena Brydon e mail: brydon@icgm.cochin.inserm.fr From owner-7tms_r@net.bio.net Wed Apr 17 23:00:00 1996 Path: biosci!CUCCFA.CCC.COLUMBIA.EDU!javitch From: javitch@CUCCFA.CCC.COLUMBIA.EDU ("Jonathan A. Javitch") Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 18 Apr 1996 14:05:31 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: <2.2.32.19960418210245.00684120@cuccfa.ccc.columbia.edu> NNTP-Posting-Host: net.bio.net subscribe ----------------------------------------------- Jonathan A. Javitch, M.D., Ph.D. Center for Molecular Recognition Columbia University P&S 11-401 630 West 168th Street New York, NY 10032 phone 212-305-3973 or 3974 fax 212-305-5594 From owner-7tms_r@net.bio.net Wed Apr 17 23:00:00 1996 Path: biosci!MAIL.UTEXAS.EDU!wilcoxrich From: wilcoxrich@MAIL.UTEXAS.EDU (Rich E. Wilcox) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Postdoc position available Date: 18 Apr 1996 09:40:34 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 59 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Colleague: This is to let you know that we have an NIAAA Training Grant Postdoctoral position available in my lab starting immediately. Salary would be $19,608 for someone with zero years experience (new postdoc), $20,700 - 1 year of relevant experience, and $25,600 - 2 years of relevant experience. Insurance is paid for the postdoc only (no family members) at the rate of $200 per month. There are no other financial benefits accruing. One of the key benefits, of course, is recognition as a trainee on an NRSA training grant. Recent Ph.D.s in Pharmacology, Neuroscience, Biochemistry, or Molecular Biology are especially encouraged to apply. The work would be broadly related to developing an improved understanding of the bases for and possibloe methods of treating craving to ethanol and other drugs of abuse. Specifics studies would involve regulation of recombinant dopamine and serotonin receptors stably expressed in clonal cells, and endogenously expressed receptors of several types expressed in astrocyte primary cultures and astrocyte clones. There are two related aspects of the studies. One is to use membrane receptor binding methods and second messenger assays in intact cells to evaluate effects of agonist and partial agonist treatments on desensitization. The other is to use small molecule - based computational chemistry methods (such as pharmacophore mapping; CoMFA, Comparative Molecular Field Analysis; 3D-chemical database searching, etc.) to evaluate structure activity relationships responsible for differences in partial agonist abilities to induce desensitization. The postdoctoral student would have the opportunity to learn or enhance their knowledge of wet lab assay methods, including nonlinear analysis of families of logistic dose-response curves, CoMFA and related computational chemistry methods incorporated in Sybyl (Tripos Associates), and have some exposure to receptor protein modeling methods. Extensive collaborative opportunities exist both with other Training Grant scientists and with other collaborators of Dr. Wilcox, both here at UT and at other institutions. Interested candidates should send their resumes to me by regular or e-mail and three letters of recommendation to me by regular mail as soon as possible. Sincerely, Richard E. Wilcox, Ph.D. Professor & Doluisio Fellow in Pharmacology Neuropharmacology Program Head Institute for Neuroscience 512-471-5199 (phone); 512-475-6088 (FAX); wilcoxrich@mail.utexas.edu (Internet) Richard E. Wilcox, Ph.D. Professor & Doluisio Fellow of Pharmacology College of Pharmacy & Institute for Neuroscience University of Texas Wichita St. Austin, TX 78712-1074 512-471-5199 FAX 512-475-6088 or 512-471-5002 From owner-7tms_r@net.bio.net Wed Apr 17 23:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news2.cais.net!news.cais.net!nntp.uio.no!nntp.uib.no!nntp-bergen.UNINETT.no!nntp-trd.UNINETT.no!daresbury!not-for-mail From: luc.camoin@cochin.inserm.fr (Luc CAMOIN) (transmis par luc.camoin@icgm.cochin.inserm.fr (Luc CAMOIN)) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Endogenous G-protein subunits Date: 18 Apr 1996 17:59:31 +0100 Lines: 20 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4l5sdj$rju@mserv1.dl.ac.uk> X-Sender: camoin@icgm.cochin.inserm.fr Original-To: 7tms_r@dl.ac.uk Dear Netters, Does anyone know of an inventory of G-protein subunits (alpha-beta-gamma) in commonly used cell lines HEK293, CHO, COS cells, etc .... Thanks for any help. Luc CAMOIN Institut _/_/_/_/ _/_/_/_/ _/_/_/_/ _/_/ _/ Luc CAMOIN Cochin _/ _/ _/ _/ _/ _/_/ CNRS UPR 415 de _/ _/ _/ _/_/ _/ _/ _/ 22 rue Mechain Genetique _/ _/ _/ _/ _/ _/ 75014 Paris France Moleculaire _/_/_/_/ _/_/_/_/ _/_/_/_/ _/ _/ Tel:(33-1) 40 51 64 98 From owner-7tms_r@net.bio.net Wed Apr 17 23:00:00 1996 Path: biosci!LILLY.COM!JOHNSON_DWAYNE From: JOHNSON_DWAYNE@LILLY.COM (JOHNSON DWAYNE) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Promiscuous coupling Date: 18 Apr 1996 11:56:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <8635521318041996.A98144.MCVAX4.11A4936E3900*@MHS> NNTP-Posting-Host: net.bio.net I am trying to determine the extent to which receptors which couple to inhibition of cyclase will couple to other second messengers. Does anyone have any information on this (references would be great) or experiences they would like to share? Thank you much- Dwayne Johnson Eli Lilly and Co. johnson_dwayne@lilly.com From owner-7tms_r@net.bio.net Thu Apr 18 23:00:00 1996 Path: biosci!LILLY.COM!JOHNSON_DWAYNE From: JOHNSON_DWAYNE@LILLY.COM (JOHNSON DWAYNE) Newsgroups: bionet.molbio.proteins.7tms_r Subject: re: Promiscuous coupling Date: 19 Apr 1996 08:14:13 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 45 Sender: daemon@net.bio.net Distribution: world Message-ID: <2305091019041996.A10502.MCVAX2.11A49A843000*@MHS> NNTP-Posting-Host: net.bio.net I have been asked to forward replies to my original request for info on promiscuous coupling of 7TMR that normally couple to Gi. Here is the first: - Dwayne Johnson Eli Lilly and Co johnson_dwayne@lilly.com -------------------------------------------------------------------------------- Dwayne, The adenosine A1 receptor is rather promiscuous and can couple to different effector systems depending upon the system being studied. Check out the following two reviews by Gary Stiles and the references that he sites. Olah ME and Stiles GL, Adenosine receptor subtypes: characterization and therapeutic regulation. Annu. Rev. Pharmacol. Toxicol. 35: 581-606, 1995. Palmer TM and Stiles GL, Adenosine receptors. Neuropharmacology 34(7): 683-694, 1995. Kenakin's work on muscarinic receptors is another example of promiscuous receptors. Kenakin T, Are receptors promiscuous? Intrinsic efficacy as a transduction phenomenon. Life Sci. 43: 1095-1101, 1988. Kenakin TP and Boselli C, Promiscuous or heterogeneous muscarinic receptors in rat atria? I. Schild analysis with simple competitive antagonists. Eur. J. Pharmacol. 191(1): 39-48, 1990. Boselli C and Kenakin TP, Promiscuous or heterogeneous muscarinic receptors in rat atria? II. Antagonism of the responses to carbacol by pirenzepine. Eur. J. Pharmacol. 191: 49-57, 1990. Please post the answers to your question, since I'm sure it will be of interest to the group. Harvey L. Wiener CNS Drug Discovery Bristol-Myers Squibb Co. Wallingford, CT 06492 (203)284-6919 -------------------------------------------------------------------------------- From owner-7tms_r@net.bio.net Fri Apr 19 23:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!oleane!plug.news.pipex.net!pipex!tank.news.pipex.net!pipex!newsfeed.internetmci.com!newsxfer2.itd.umich.edu!news.itd.umich.edu!usenet From: Rick Neubig Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Promiscuous coupling Date: Sat, 20 Apr 1996 07:02:32 -0400 Organization: University of Michigan Lines: 35 Message-ID: <3178C448.5562@umich.edu> References: <8635521318041996.A98144.MCVAX4.11A4936E3900*@MHS> NNTP-Posting-Host: host-220.subnet-40.med.umich.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win95; I) Dwayne, Gi-linked receptors are notorious for having multiple mechanisms. This was probably first discussed in detail by Lee Limbird. Limbird, L.E. Receptors linked to inhibition of adenylate cyclase: additional signaling mechansims. FASEB J. 2:2686-2695, 1988. The question you ask has some interesting mechanistic implications in that the "promiscuity" could be due to coupling to multiple G alpha subunits in addition to alpha-i (as has been shown for thrombin receptors which activate alpha-q and alpha-12 also) or by effects of both the alpha-i (e.g. inhibition of adenylyl cyclase) and beta-gamma (e.g. cyclase inhibition, PLC-beta activation, GIRK activation and more recently Ca channel inhibition - last week's Nature). Rick JOHNSON DWAYNE wrote: > > I am trying to determine the extent to which receptors which couple to > inhibition of cyclase will couple to other second messengers. Does anyone have > any information on this (references would be great) or experiences they would > like to share? Thank you much- > > Dwayne Johnson > Eli Lilly and Co. > johnson_dwayne@lilly.com -- _________________________________________________________ Rick Neubig RNeubig@umich.edu Department of Pharmacology University of Michigan Phone (313) 763-3650 FAX (313) 763-4450 http://www-personal.umich.edu/~rneubig/ From owner-7tms_r@net.bio.net Mon Apr 22 23:00:00 1996 Path: biosci!PHARM.MED.UPENN.EDU!butkerai From: butkerai@PHARM.MED.UPENN.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Promiscuous coupling Date: 23 Apr 1996 07:31:00 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <199604231423.KAA21230@pharm.med.upenn.edu> NNTP-Posting-Host: net.bio.net Dwayne, In my experience with the 5-HT1A receptor (JBC 270:18691-99, 1995) I found that it can couple to all of the members of the Gi "family" when coexpressed in Sf9 cells but it does not couple to members of other G protein families (Gs, Gq). So in this reconstituted system I don't see promiscuity at the level of the alpha subunit. So what determines promiscuity is a good question. *************************** Paul Butkerait, Ph.D. Dept. of Pharmacology University of Pennsylvania butkerai@pharm.med.upenn.edu tel: 215-898-9769 fax: 215-573-2236 *************************** From owner-7tms_r@net.bio.net Thu Apr 25 23:00:00 1996 Path: biosci!HLSUN.RED-CROSS.ORG!hlatim From: hlatim@HLSUN.RED-CROSS.ORG ("Timothy Hla") Newsgroups: bionet.molbio.proteins.7tms_r Subject: test Date: 26 Apr 1996 19:59:17 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: <9604262020.AA01778@hlsun.red-cross.org> Reply-To: "Timothy Hla" NNTP-Posting-Host: net.bio.net From owner-7tms_r@net.bio.net Thu Apr 25 23:00:00 1996 Path: biosci!HLSUN.RED-CROSS.ORG!hlatim From: hlatim@HLSUN.RED-CROSS.ORG ("Timothy Hla") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Postdoctoral Position Available Immediately Date: 26 Apr 1996 20:14:54 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 35 Sender: daemon@net.bio.net Distribution: world Message-ID: <9604251848.AA24307@hlsun.red-cross.org> Reply-To: "Timothy Hla" NNTP-Posting-Host: net.bio.net Postdoctoral Training in Vascular Cell and Molecular Biology Postdoctoral positions are currently available in the laboratory of Dr. Timothy Hla to study the function of unique early response genes, edg-1 and Cox 2 from endothelial cells. The function of these gene products, an inducible G protein-coupled receptor and the inducible cyclooxygenase enzyme are being investigated at the cellular level using molecular methods and at the organism level using transgenic technology. Recent publications include: JBC 271, 11272- 11279 (1996); Cancer Res. 55, 3785-3789 (1995); JBC 269, 11769-11775 (1994); JCI 93, 1095-1101 (1994). Interested individuals with previous experience in cell and molecular biology/ biochemistry/ genetics should send CV, relevant publications and names of three referees to: Timothy Hla, Ph.D. Department of Molecular Biology Holland Laboratory, American Red Cross 15601 Crabbs Branch Way Rockville, MD 20855 ph: 301-738-0567 fx: 301-738-0465 e-mail: hlatim@hlsun.red-cross.org after August 1, 1996 please write to the following address: Department of Physiology University of Connecticut Health Center School of Medicine 263 Farmington Avenue Farmington, CT 06032 From owner-7tms_r@net.bio.net Sat Apr 27 23:00:00 1996 Path: biosci!bcm.tmc.edu!news.uth.tmc.edu!news.uh.edu!uuneo.neosoft.com!winnie-slip-i1 From: shark@sam.neosoft.com (John D. Valentich) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Biologists in the Matrix: List your lab in this new index! Date: 29 Apr 1996 03:38:10 GMT Organization: University of Texas Medical Branch Lines: 36 Message-ID: <4m1dj2$o4h@uuneo.neosoft.com> NNTP-Posting-Host: winnie-slip-i1.neosoft.com X-Newsreader: News Xpress Version 1.0 Beta #3 To foster worldwide collaborative interactions between biomedical researchers on the Internet, Biologists in the Matrix has been created. It is available on the Web at: http://www.neosoft.com/~shark/bim1.htm Biologists in the Matrix is an index of Web sites where investigators post their scientific interests and research programs. To help Biologists in the Matrix attain the critical mass necessary to become a useful resource, you are invited to provide the URL of your personal or lab's Web home page for inclusion in the index. A form to submit this information is found at: http://www.neosoft.com/~shark/bimform1.htm Additional information about Biologists in the Matrix is available at: http://www.neosoft.com/~shark/faq1.htm If you encounter any problems accessing or interacting with the above pages, email me at the address below. ************************************************************************** John D. Valentich, Ph.D. Email: shark@sam.neosoft.com Web Site: http://www.neosoft.com/~shark/reef.html Department of Internal Medicine Voice: (409)772-9887 University of Texas Medical Branch FAX: (409)772-3394 301 University Blvd. Galveston, TX 77555-0564 "Let's go exploring!".... Calvin's last words to Hobbes