From owner-7tms_r@net.bio.net Tue Jul 02 23:00:00 1996 Path: biosci!NEWSSUN.MED.MIAMI.EDU!vslepak From: vslepak@NEWSSUN.MED.MIAMI.EDU ("Vladlen Z. Slepak") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Postdoctoral Position in University of Miami Date: 3 Jul 1996 15:04:03 -0700 Organization: University of Miami Medical School Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <31DAED7D.46BD@newssun.med.miami.edu> Reply-To: vslepak@newssun.med.miami.edu NNTP-Posting-Host: net.bio.net POSTDOCTORAL POSITION is available immideately at the UNIVERSITY OF MIAMI SCHOOL OF MEDICINE. Successful candidate will join a new, well-funded and equipped lab studying G protein-mediated signal transduction mechanisms. Projects involve protein isolation from native sources, mutagenesis and heterologous expression of proteins, kinetic analysis of protein-protein interactions using Surface Plasmon Resonance (BIAcore instrument) and other approaches. Background in the area of cellular signaling and expertise in protein-protein interactions and molecular biology is preferred, but is not as essential as strong commitment to career in science and ability to work independently. Future promotion to a more independent, research-track faculty position and obtaining technical support is achievable. Salary is negotiable and commensurate with experience. Send CV and three references to: Dr. V. Z. Slepak, Department of Pharmacology, University of Miami School of Medicine, 1600 N.W. 10th Avenue. R-189 Miami, Fl 33136. fax: (305) 243-4555, E.mail From owner-7tms_r@net.bio.net Tue Jul 02 23:00:00 1996 Path: biosci!MXA.MESHNET.OR.JP!yoshicad From: yoshicad@MXA.MESHNET.OR.JP Newsgroups: bionet.molbio.proteins.7tms_r Subject: mAChRs-pirenzepine Date: 3 Jul 1996 20:01:00 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: <31DB3336.5A98@mxa.meshnet.or.jp> NNTP-Posting-Host: net.bio.net Dear Netters: I'm interested in modeling mAChRs based on bacteriorhodopsin. With regard to docking antagonist "pirenzepine", Nichlas J.M.'s recent report ( Biooragnic & Medicinal Chemistry Letters, 6, 785,1996 ) was very helpful for me. They reported the diffrence in ligand specificity of mAChRs. They suggest the interaction between Thr32(in helix 1) and pirenzepine would be involved. Although I have no experimental data, I think that Glu401(in helix 7) rather than Thr32 would be involved in the pirenzepine recognition. Any suggentions, solutions would be appreciated. Thanks all Yoshitaka Fukumasu -- Yoshitaka Fukumasu( Yoshitomi Pharmaceutical Industries, LTD ) 955 Koiwai, Yoshitomi-cho, Chikujo-gun, Fukuoka, JAPAN 871 e-mail: yoshicad@mxa.meshnet.or.jp Fax: +81-979-24-3127 Phone: +81-979-23-8974(direct) From owner-7tms_r@net.bio.net Wed Jul 03 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!howland.reston.ans.net!newsfeed.internetmci.com!in1.uu.net!munnari.OZ.AU!news.unimelb.EDU.AU!tracy From: tracy@austin.unimelb.edu.au (Tracy Nero) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Australian Medicinal Chemistry Conference Dec 96 Date: 4 Jul 1996 05:54:40 GMT Organization: Melbourne University, Victoria, Australia Lines: 86 Sender: tracy@gjok@austin.unimelb.edu.au (Tracy Nero) Distribution: world Message-ID: <4rfmb0$8ar@news.unimelb.EDU.AU> NNTP-Posting-Host: tango.austin.unimelb.edu.au Please excuse multiple copies due to cross posting. ROYAL AUSTRALIAN CHEMICAL INSTITUTE MEDICINAL & AGRICULTURAL DIVISION 13TH NATIONAL CONFERENCE "UP AND COMING RESEARCH IN AUSTRALIA" 8-11 DECEMBER, 1996, Monash University, Clayton 3168, Victoria, Australia The conference will cover all aspects of medicinal and agricultural chemistry with a focus on: o students and new researchers o emerging research areas in medicinal and agricultural chemistry o the interaction between medicinal chemists, pharmacologists and industry. Papers are invited for both lecture and poster sessions. The meeting will be held in conjunction with the ASCEPT (Australian Society of Clinical and Experimental Pharmacologists and Toxicologists) and APPS (Australian Physiological and Pharmacological Society) conferences. There will be a joint social mixer and workshop on the initiation of research/industry interactions on sunday evening. Monday morning will feature a joint session on mass screening and combinatorial chemistry, followed in the evening by a joint poster session and wine tasting. Invited Speakers Include: Preliminary Session Topics Include: o Dr George Fleet - glycochemistry, o agrochemistry herbicides,tuberculosis o glycobiology and glycochemistry (Oxford University, UK) o toxins o Dr David Manallack - metalloproteins, o anti-infectives neural networks (Chiroscience, UK) o enzyme inhibitors o Dr Lia Addadi - biomineralization o QSAR methods (Weizman Institute, Israel) o automated methods of o Dr Shiela Unkles - fungal enzymes synthesis and screening (Monash University, Aus) o DNA and drugs o Dr Ron Quinn - mass screening o medicinal chemistry teaching (Griffith University, Aus) o Dr John Bremner - medicinal chemistry teaching (Uni. of Wollongong, Aus) Social Events: o Dr Mohan Venkataraman - DNA drugs o mixer sunday evening (ISIS Pharmaceuticals, USA) o wine tasting and mixer after o Dr Paul Rowland - drug poster session monday evening development (Medeval Ltd., UK) o conference dinner at Kenloch o Dr Damon Ridley - chemical restaurant in the Dandenong Ranges databases (Uni. of Sydney, Aus) (dinner cost for accompanying person $60) o Prof Colin Masters - prions, Alzheimers disease (Uni. of Melbourne, Aus) o Prof William Denny - cancer research (Auckland University, NZ) Student Scholarships: 10 scholarships of $250 will be available to interstate students presenting either a talk or poster Deadlines: Abstract 30th Sept., 1996 Registration 8th Nov., 1996 Registration Costs: On or before 8th Nov After 8th Nov RACI Member $300 $400 non-RACI Member $375 $475 Reciprocal Society Member $300 $400 RACI Student $100 $200 non-RACI Student $140 $240 NOTE 1: Above costs are all in AUSTRALIAN DOLLARS NOTE 2: All social mixers,the conference dinner, workshop, morning & afternoon teas and boxed lunches are included in the registration cost To receive the conference circular and registration form contact: o the conference organiser Dr Mick Gould, Conference Associates Pty Ltd, 13 Jeffrey St, Mt Waverly, Victoria, Australia 3149. Ph: (03) 9887-8003 Fax: (03) 9887-8773 email: ca@netwide.com.au o Dr. Margaret Wong, Department of Applied Chemistry,Swinburne University of Technology, Hawthorn,Victoria, Australia 3122. Ph: (03) 9214-8542 Fax: (03) 9819-0834 email: marg@chem1.chem.swin.edu.au o Web page http://www.chem.swin.edu.au/ma/mconf.html (registration forms will be available from the web site at a later date) From owner-7tms_r@net.bio.net Mon Jul 08 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!news.cic.net!news.itd.umich.edu!usenet From: Rick Neubig Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: AT1-receptors Date: Tue, 09 Jul 1996 18:21:32 -0400 Organization: University of Michigan Lines: 20 Message-ID: <31E2DB6C.4375@umich.edu> References: Reply-To: RNeubig@umich.edu NNTP-Posting-Host: warbler.med.umich.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0b4Gold (Win95; I) To: David Port David Port wrote: > > As a follow up question, does anyone know if any readily available cell lines > (not primary culture) express Ang II AT-1 receptors? > > Thanks, > > david.port@uchsc.edu David, Rat Clone 9 cells (I believe from liver) express AT1 receptors. I think this is cited in: 1. Dudley, D.T., Panek, R.L., Major, T.C., Lu, G.H., Bruns, R.F., Klinkefus, B.A., Hodges, J.C., and Weishaar, R.E. Subclasses of Angiotensin II binding sites and their functional significance. Mol.Pharmacol 38:370-377, 1990. Rick From owner-7tms_r@net.bio.net Mon Jul 08 23:00:00 1996 Path: biosci!UCHSC.EDU!David.Port From: David.Port@UCHSC.EDU ("David Port") Newsgroups: bionet.molbio.proteins.7tms_r Subject: AT1-receptors Date: 9 Jul 1996 14:42:48 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net As a follow up question, does anyone know if any readily available cell lines (not primary culture) express Ang II AT-1 receptors? Thanks, david.port@uchsc.edu From owner-7tms_r@net.bio.net Mon Jul 08 23:00:00 1996 Path: biosci!UCHSC.EDU!David.Port From: David.Port@UCHSC.EDU ("David Port") Newsgroups: bionet.molbio.proteins.7tms_r Subject: AT-1 receptors Date: 9 Jul 1996 14:12:45 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Does anyone out there know if hamster DDT1-MF2 cells express angiotensin II AT-1 receptors? Thanks, david.port@uchsc.edu From owner-7tms_r@net.bio.net Mon Jul 08 23:00:00 1996 Path: biosci!UCHSC.EDU!David.Port From: David.Port@UCHSC.EDU ("David Port") Newsgroups: bionet.molbio.proteins.7tms_r Subject: AT-1 receptors Date: 9 Jul 1996 14:12:43 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Does anyone out there know if hamster DDT1-MF2 cells express angiotensin II AT-1 receptors? Thanks, david.port@uchsc.edu From owner-7tms_r@net.bio.net Mon Jul 08 23:00:00 1996 Path: biosci!rutgers!uwm.edu!spool.mu.edu!usenet.eel.ufl.edu!news-feed-1.peachnet.edu!news.service.emory.edu!news From: Brett Burkholder Newsgroups: bionet.molbio.proteins,bionet.molbio.proteins.7tms_r,bionet.molbio.yeast Subject: Re: Yeast antibodies!! Date: Tue, 09 Jul 1996 16:06:02 +0000 Organization: Emory University Lines: 36 Message-ID: <31E2836A.645@gmm.gen.emory.edu> References: <4qpl38$kt3$1@mhadf.production.compuserve.com> <4qv8v7$ef9@mark.ucdavis.edu> NNTP-Posting-Host: djdgw486.gen.emory.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01 (Win16; I) Xref: biosci bionet.molbio.proteins:8289 bionet.molbio.proteins.7tms_r:770 bionet.molbio.yeast:5510 Jonathan Mazer wrote: > > DSF (76042.532@CompuServe.COM) wrote: > : I'm desperately looking for commercially available antibodies to > : ubiquitously expressed yeast proteins, for instance, anti-TATA > : Binding Protein. HELP!! > : Lynette > > I'm afraid you're out of luck. There are no known commercially > available antibodies to yeast proteins that I know about. Your best bet > is to check the literature for a lab that will have made it's own > antibody(ies) against something similar to what you are working on or > what you need. Write or email them and ask for it. Explain, of course, > that you are not trying to copy their work with their antibody. Most labs > will be willing to help. (It will take a little more time, but it should > work.) Good Luck. > > -jsmazer@ucdavis.edu > This is a good suggestion that may work well for you. There may also be a commercial alternative: StressGen (1-800-661-4978, Victoria, BC) carries antibodies for chaperone proteins. For two of these antibodies, one for HSP60 and one for HSP90, StressGen claims cross reactivity with yeast extracts. Since these proteins are ubiquitous and highly conserved, one or both of these may serve you well. These protiens are ubiquitously expressed, but expression is increased under stressful conditions (i.e. extreme temperatures and certain media). That may be the biggest drawback to using them. Brett Burkholder Emory University Genetics and Molecular Medicine From owner-7tms_r@net.bio.net Tue Jul 09 23:00:00 1996 Path: biosci!GREENCROSS.CO.JP!inoue From: inoue@GREENCROSS.CO.JP (Yoshihisa Inoue) Newsgroups: bionet.molbio.proteins.7tms_r Subject: model list part.5 Date: 10 Jul 1996 03:37:09 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 252 Sender: daemon@net.bio.net Distribution: world Message-ID: <199607101037.TAA00934@greencross.co.jp> NNTP-Posting-Host: net.bio.net AU W.L.DeLano and A.Brunger TI Helix packing in Proteins: Prediction and energetic analysis of dimeric, trimeric, and tetrameric GCN4 coiled coil structures AU N.Unwin TI Acetylcholine receptor channel imaged in the open state SO Nature,373,37-43 (1995) AU S.-K. Choi, A.G.Kalivretenos, P.N.R.Usherwood, and K.Nakanishi TI Labeling studies of photolabile philanthotoxins with nicotinic acetylcholine receptors: mode of interaction between toxin and receptor SO Chemistry and Biology, 2,23-32 (1995) **************************************************************** AU M.Williams, D.C.Deecher and J.P.Sullivan TI Drug Receptors SO Burger's Medicinal Chemistry and Drug Discovery, 5th Eds. vol.1 Principles and Practice, Edited by M.E.Wolff. John Wiley and Sons, Inc. (1995). COM generic model built by Dr.C.Hutchins, Abbott Lab. AU Y.Liu, H.Yu, N.Mohell, G.Nordvall, T.Lewander, and U.Hacksell TI Derivatives of cis-2-amino-8-hydroxy-1-methyltetralin: Mixed 5-HT1a-receptor agonists and Dopamine D2-receptor antagonists SO J.Med.Chem.,38,150-160 (1995) AU B.M.Nilsson, S.Sundquist, G.Johansson, G.Nordvall, G.Glas, L.Nilvebrant, and U.Hacksell TI 3-Heteroaryl-Substituted Quinuclidin-3-ol and Quinuclidin-2-ene Derivatives as Muscarinic Antagonists. Synthesis and Structure- Activity Relationship SO J.Med.Chem.,38,473-487 (1995) AU M.H.Hedberg, A.M.Johansson, G.Nordvall, A.Yliniemela, H.B.Li, A.R.Martin, S.Hjorth, L.Unelius, S.Sundell, and U.Hacksell TI (R)-11-Hydroxy- and (R)-11-Hydroxy-10-methylaporphine: Synthesis, Pharmacology, and Modeling of D2A and 5-HT1A receptor interactions SO J.Med.Chem.,38,647-658 (1995) AU W.Kuipers, I.van Wijngaarden, C.G.Kruse, M.ter Horst-van Amstel, M.T.M.Tulp, and A.P.IJzerman TI N4-unsubstituted N1-Arylpiperazines as High-Affinity 5-HT1A Receptor Ligands SO J.Med.Chem.,38,1942-1954 (1995). AU F.Fanelli, M.C.Menziani, M.Cocchi, and P.G.De Benedetti TI Comparative Molecular Dynamics Study of the Seven-helix Bundle Arrangement of G-protein Coupled Receptors. SO J.Mol.Struct.(Theochem),333,49-69 (1995) Com alpha.1B, alpha.2, beta2, Dopamine-D2, 5-HT1A, muscarine-M1 AU M.C.Menziani, M.Cocchi, F.Fanelli, and P.G.De Benedetti TI Quantitative Structure-affinity/selectivity Relationship Analysis on Three-Dimensional Models of the Complexes between the ETa and ETb Receptors and C-terminal Endothelin Hexapeptide Antagonists. SO J.Mol.Struct.(Theochem),333,243-248 (1995) AU P.Prusis, P.-A.Fraendberg, R.Muceniece, I.Kalvinsh, and J.E.S.Wikberg TI A Three Dimensional Model for the Interaction of MSH with the Melanocortin-1 Receptor SO Biochem.Biophys.Res.Commun.,210,205-210 (1995) AU R.J.Knapp, E.Malatynska, N.Collins, L.Fang, J.Y.Wang, V.J.Hruby W.R.Roeske, and H.I.Yamamura TI Molecular Biology and Pharmacology of Cloned Opioid Receptors SO FASEB J.,9,516-525 (1995) AU A.Vedani, P.Zbinden, J.P.Snyder, and P.A.Greenridge TI Pseudoreceptor Modeling: The Construction of Three-Dimensional Receptor Surrogates SO J.Am.Chem.Soc.,117,4987-4994 (1995) COM beta2 receptor and dopaminergic receptor AU I.Logunov, W.Humphrey, K.Schulten, and M.Sheves TI Molecular Dynamics Study of the 13-Cis Form (bR548) of Bacteriorhodopsin and its Photocycle SO Biophys.J.,68,1270-1282 (1995) AU M.S.P.Sansom, H.S.Son, R.Sankararamakrishnan, I.D.Kerr, and J.Breed TI Seven-Helix Bundles: Molecular Modeling via Restrained Dynamics. SO Biophys.J.,68,1295-1310 (1995) AU Diane Hope Peapus TI Crystallographic Characterization of Human Endothelin and Theoretical Studies of Membrane Protein Structure SO Diss. Abstr. Int. B.,55(8), 3295 (1995). or Chem.Abstr.,122(21),258964h Com 248pp(1994). Location: Rensselaer Polytech. Inst., Troy, NY, USA. available from University Microfilms Int., Order No. DA9434587. AU J.Kim, J.Wess, A.M.Rhee, T.Schoneberg, and K.A.Jacobson TI Site-directed mutagenesis identifies residues involved in ligand recognition in the human A2a adenosine receptor SO J.Biol.Chem., 270, 13987-1399x (1995) AU Y.Yamano, K.Ohyama, M.Kikyo, T.Sano, Y.Nakagomi, Y.Inoue, N.Nakamura, I.Morishima, D.F.Guo, T.Hamakubo, and T.Inagami TI Mutagenesis and the molecular modeling of the rat angiotensin II receptor (AT1) SO J.Biol.Chem., 270, 14024-14029 (1995) AU B.Rost, R.Casadio, P.Fariselli, and C.Sander TI Transmembrane Helices Predicted at 95% Accuracy SO Protein Sci.,4,521-533 (1995) Com Theory and prediction of the transmembrane spanning sequence AU G.Offer, and R.Sessions TI Computer Modelling of the alpha.-Helical Coiled Coil: Packing of Side-chains in the Inner Core SO J.Mol.Biol.,249,967-987 (1995). Com coiled-coil with the sequence (LAALAAA)5 AU R.Sankararamakrishnan, and M.S.P.Sansom TI Structural Features of Isolated M2 Helixes of Nicotinic Receptors. Simulated Annealing via Molecular Dynamics Studies SO Biophys.Chem.,55(3),215-230 (1995). Com M2 AU F.Milpetz, P.Argos, and B.Persson TI TMAP: A New Email and WWW service for Membrane-Protein Structural Predictions SO Trends Biochem.Sci.,20(5),204-205 (1995). AU C.E.Peishoff, J.S.Brinkmann, J.D.Elliot, M.A.Lago, E.D.Longton, and J.A.Lee TI Molecular Modeling and Protein Engineering: Identification and Characterization of Binding Sites within the G-Protein Coupled Endothelin Receptors. SO 210th Amer.Chem.Soc.Natl.Meeting,COMP-013,Chicago, 1995 AU T.Mirzadegan TI Factors That Affect the Relative Stability of Amino Acid Tautomers in Homology Modeling of Drug Targets. SO 210th Amer.Chem.Soc.Natl.Meeting,COMP-014,Chicago, 1995 AU N.J.Murgolo, J.E.Brown, M.A.B.Tice, J.Koslowski, F.Hollinger, R.D.McQuade, and M.Snow TI Docking of the Selective Antagonist Pirenzepine to a Human m1 Muscarinic Receptor Homology Model SO 210th Amer.Chem.Soc.Natl.Meeting,COMP-015,Chicago, 1995 Com m1 AU T.G.Metzger, D.M.Ferguson TI Structural and Agonist Binding Site Model of the k-opioid Receptor SO 210th Amer.Chem.Soc.Natl.Meeting,COMP-016,Chicago, 1995 AU Y.-D.Gao, R.J.Loncharich, M.O.Chancy, M.P.Johnson, and D.L.Nelson TI Homology Modeling of the 5-HT2A Receptor: Origin of Ligand Binding SO 210th Amer.Chem.Soc.Natl.Meeting,COMP-017,Chicago, 1995 AU T.R.Stouch, and S.R.Krystek,Jr. TI Three Dimensional Quantitative Structure-Activity Relationships of Sulfonamide Endothelin Inhibitors. SO 210th Amer.Chem.Soc.Natl.Meeting,COMP-024,Chicago, 1995 Com Tyr129 in the ETa receptor as a key residue AU W.R.Moyle, R.K.Campbell, S.N.V.Rao, N.G.Ayad, M.P.Bernard, Y.Han, and Y.Wang TI Model of Human Chorionic Gonadotropin and Lutropin Receptor Interaction that Explains Signal Transduction of the Glycoprotein Hormones SO J.Biol.Chem.,270.20020-20031 (1995). AU A.M.ter Laak, H.Timmerman, R.Leurs, P.H.J.Nederkoorn, M.J.Smit and G.M.D. den Kelder TI Modelling and Mutation Studies on the Histamine H1-Receptor Agonist Binding Site Reveal Different Binding Modes for H1-Agonists: Asp116(TM3) has a Constitutive Role in Receptor Stimulation SO J.Comp.-Aided Molec.Design, 9,319-330 (1995). AU J.B.Summers, S.K.Davidsen, and G.S.Sheppard TI Platelet Activating Factor Antagonists SO Current Pharmaceutical Design, 1,161-190 (1995). AU D.J.Kyle TI Structure-Based Drug Design: Progress Toward the Discovery of the Elusive Bradykinin Receptor Antagonists SO Current Pharmaceutical Design, 1,233-254 (1995). AU T.Hirokawa, M.Suwa, and S.Mitaku TI Theoretical prediction of positioning, orientation and tilt angles of helixes of a small membrane protein without structural template SO Genome Inf. Ser., 6(Genome Informatics Workshop 1995),128-9 (1995). com I did not read this article yet. AU S.Dove, R.Kuhne and W.Schunack TI H1 Agonistic 2-Heteroaryl and 2-Phenylhistamines: CoMFA and Possible Receptor Binding Sites SO QSAR and Mol.Modelling, pp427-432 (1995), Prous Sci.Publ. AU H.Weinstein and D.Zhang TI Receptor Models and Ligand-Induced Responses: New Insights for Structure-Activity Relations SO QSAR and Mol.Modelling, pp497-507 (1995), Prous Sci.Publ. Com 5-HT2 model AU M.F.Hibert, J.Hoflack, S.Trumpp-Kallmeyer, J.-L.Paquet and R.Leppik TI 3D Models of Hormone Receptors: Experimental Validation SO QSAR and Mol.Modelling, pp508-513 (1995), Prous Sci.Publ. Com Schematic representation of Dopamine,Adrenaline,Acetylcholine, and Serotonin Receptors. AU H.Bourdon, S.Trumpp-Kallmeyer, J.Hoflack, M.Hibert, and C.G.Wermuth TI Modelling of Muscarinic M1 Agonists. Study of Their Interaction with the M1 Receptor SO QSAR and Mol.Modelling, pp514-518 (1995), Prous Sci.Publ. AU M.C.Menziani, M.Cocchi, F.Fanelli, and P.G.De Benedetti TI Theoretical QSAR Analysis on Three Dimensional Models of the Complexes between Peptides and Non-peptide Antagonists with the ETa and ETb Receptors SO QSAR and Mol.Modelling, pp519-525 (1995), Prous Sci.Publ. AU P.G.De Benedetti, M.C.Menziani, F.Fanelli, and M.Cocchi TI The Heuristic-Direct Approach to QSAR Analysis of Ligand- G-Protein Coupled Receptor Complexes SO QSAR and Mol.Modelling, pp526-527 (1995), Prous Sci.Publ. Com Rhodopsin model AU T.L.Nero, D.Iakovidis, and W.J.Louis TI Molecular Modelling of the Human .beta1.-Adrenoceptor SO QSAR and Mol.Modelling, pp528-530 (1995), Prous Sci.Publ. AU G.Krause, R.Kuehne, and S.Huebel TI G-Protein Coupled Receptors, Glucagon Type: How to Overcome the Alignment/Fit Dilemma to the Bacteriorhodopsin Template SO QSAR and Mol.Modelling, pp531-533 (1995), Prous Sci.Publ. AU M.Batlle, M.Campillo, J.Giraldo, and L.Pardo TI Computer-Aided Drug Design of Selective 5-Hydroxytryptamine-1a Receptor Ligands Using a Three-dimensional Model SO QSAR and Mol.Modelling, pp541-544 (1995), Prous Sci.Publ. AU M.N.Iskander, R.De Gori, and M.G.Wong TI A Proposed Active Site for GABAa Receptor Antagonist SO QSAR and Mol.Modelling, pp556-561 (1995), Prous Sci.Publ. Com Only active sites were shown AU M.Laguerre, M.Saux, and A.Carpy TI Molecular Lipophilicity Potential and Proteins Structure SO QSAR and Mol.Modelling, pp588-591 (1995), Prous Sci.Publ. Com Bacteriorhodopsin ____/ ___/ ___/ Yoshihisa INOUE (^_^) the Green Cross Corp. / / / 2-25-1 Shodai-Ohtani,Hirakata,Osaka 573 JAPAN / _ / / / tel: +81-720-56-9328 / / / / fax: +81-720-68-9597 _____/ _____/_____/ E-mail: inoue@greencross.co.jp From owner-7tms_r@net.bio.net Tue Jul 09 23:00:00 1996 Path: biosci!GREENCROSS.CO.JP!inoue From: inoue@GREENCROSS.CO.JP (Yoshihisa Inoue) Newsgroups: bionet.molbio.proteins.7tms_r Subject: model list part.6 Date: 10 Jul 1996 03:38:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 71 Sender: daemon@net.bio.net Distribution: world Message-ID: <199607101039.TAA00941@greencross.co.jp> NNTP-Posting-Host: net.bio.net AU A.Cappelli, M.Anzini, S.Vomero, M.C.Menziani, P.G.de Benedetti, M.Sbacchi, G.D.Clarke, and L.Mennuni TI Synthesis, Biological Evaluation, and Quantitative Receptor Docking Simulations of 2-[(Acylamino)ethyl]-1,4-benzodiazepines as Novel Tifluadom-like Ligands with High Affinity and Selectivity for kappa-opioid Receptors SO J.Med.Chem.,39,860-872 (1996). Com kappa opioid receptor AU T.W.von Geldern, C.Hutchins, J.A.Kester, J.R.Wu-Wong, W.Chiou, D.B.Dixon, and T.J.Opgenorth TI Azole Endothelin Antagonists.1. A Receptor Model Explains an Unusual Structure-Activity Profile SO J.Med.Chem.,39,957-967 (1996). AU H.S.Son, and M.S.P.Sansom TI Simulation of the packing of Transmembrane .alpha.-helixes SO Biochem.Soc.Trans., 24(1),140S (1996). AU R.Casadio, R.Fariselli, C.Taroni, and M.Compiani TI A Predictor of Transmembrane .alpha.-helix Domain of Proteins based on Neural Networks SO Eur.Biophys.J., 24(3), 165-178 (1996). AU A.V.Kajava TI Modeling of a Five-Stranded Coiled Coil Structure for the Assembly Domain of the Cartilage Oligomeric Matrix Protein SO Proteins: Structure, Function, and Genetics, 24,218-226(1996). Com 5 helices model AU M.O.Ortells and G.G.Lunt TI A Mixed Helix-beta-sheet Model of the Transmembrane Region of the Nicotinic Acetylcholine Receptor SO Protein Eng., 9(1),51-59 (1996). Com not 7-transmembrane receptor AU I.Sylte, O.Edvardsen and S.G.Dahl TI Molecular Modelling of UH-301 and 5-HT1a Receptor Interactions SO Protein Eng., 9(2),149-160 (1996). AU I.D.Kerr, D.G.Doak, R.Sankararamakrishnan, J.Breed and M.S.P.Sansom TI Molecular Modelling of Staphylococcal .delta.-toxin Ion Channels by Restrained Molecular Dynamics SO Protein Eng., 9(2),161-171 (1996). Com 6-helices model = ion channel AU R.G.Efremov and G.Bergoten TI Recognition of Transmembrane .alpha.-Helical Segments with Environmental Profiles SO Protein Eng., 9(3),253-263 (1996). Com not showing model, but theoretically (3D-1D) AU O.D.Monera, F.D.Sonnichsen, L.Hicks, C.M.Kay and R.S.Hodges TI The Relative Positions of Alanine Residues in the Hydrophobic Core Control the Formation of Two-stranded or four-stranded .alpha.-Helical Coiled-coils SO Protein Eng., 9(4),353-363 (1996). Com 4-helices model AU N.Grigorieff, T.A.Ceska, K.H.Downing, L.M.Baldwin, and R.Henderson TI Electron-crystallographic Refinement of the Structure of Bacteriorhodopsin SO J.Mol.Biol.,259, 393-421 (1996). ____/ ___/ ___/ Yoshihisa INOUE (^_^) the Green Cross Corp. / / / 2-25-1 Shodai-Ohtani,Hirakata,Osaka 573 JAPAN / _ / / / tel: +81-720-56-9328 / / / / fax: +81-720-68-9597 _____/ _____/_____/ E-mail: inoue@greencross.co.jp From owner-7tms_r@net.bio.net Tue Jul 09 23:00:00 1996 Path: biosci!GREENCROSS.CO.JP!inoue From: inoue@GREENCROSS.CO.JP (Yoshihisa Inoue) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: mAChRs-pirenzepine Date: 10 Jul 1996 03:33:10 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 58 Sender: daemon@net.bio.net Distribution: world Message-ID: <960710193408.M0100901@gccss2.greencross.co.jp> References: <31DB3336.5A98@mxa.meshnet.or.jp> NNTP-Posting-Host: net.bio.net Hello Dr.Fukumasu, Please refer the following reports. AU G.Shapiro, P.Floersheim, J.Boelsterli, R.Amstutz, G.Bolliger, H.Gammenthaler, G.Gmelin, P.Supavilai, and M.Walkinshaw TI Muscarinic activity of the thiolactone, lactam, Lactol, and thiolactol analogues of pilocarpine and a hypothetical model for the binding of agonists to the m1 receptor SO J.Med.Chem.,35,15-27 (1992) COM m1 receptor AU J.S.Ward, L.Merritt, V.J.Klimkowski, M.L.Lamb, C.H.Mitch, F.P.Bymaster, B.Sawyer, H.E.Shannon, P.H.Olessen, T.Honore, M.J.Sheardown, and P.Sauerberg TI Novel Functional M1 selective muscarinic agonists.2. Synthesis and Structure-activity relationships of 3-pyrazinyl-1,2,5,6-tetrahydro-1- methylpyridines. Construction of a molecular model for the M1 Pharmacophore SO J.Med.Chem.,35,4011-4019 (1992) COM m1 receptor AU G.Nordvall, and U.Hacksell TI Binding-site modeling of the muscarinic m1 receptor: a combination of homology-based and indirect approaches SO J.Med.Chem.,36,967-976 (1993) COM precise methodology, and superposition of the kinked helices containing proline. supplementary material available AU F.Fanelli, M.C.Menziani, M.Cocchi, and P.G.De Benedetti TI Comparative Molecular Dynamics Study of the Seven-helix Bundle Arrangement of G-protein Coupled Receptors. SO J.Mol.Struct.(Theochem),333,49-69 (1995) Com alpha.1B, alpha.2, beta2, Dopamine-D2, 5-HT1A, muscarine-M1 AU H.Bourdon, S.Trumpp-Kallmeyer, J.Hoflack, M.Hibert, and C.G.Wermuth TI Modelling of Muscarinic M1 Agonists. Study of Their Interaction with the M1 Receptor SO QSAR and Mol.Modelling, pp514-518 (1995), Prous Sci.Publ. AU B.M.Nilsson, S.Sundquist, G.Johansson, G.Nordvall, G.Glas, L.Nilvebrant, and U.Hacksell TI 3-Heteroaryl-Substituted Quinuclidin-3-ol and Quinuclidin-2-ene Derivatives as Muscarinic Antagonists. Synthesis and Structure- Activity Relationship SO J.Med.Chem.,38,473-487 (1995) I did not check the 3-dimensional model, but it seems that Glu401 would be involved in the pirenzepine recognition. Hope this help. ____/ ___/ ___/ Yoshihisa INOUE (^_^) the Green Cross Corp. / / / 2-25-1 Shodai-Ohtani,Hirakata,Osaka 573 JAPAN / _ / / / tel: +81-720-56-9328 / / / / fax: +81-720-68-9597 _____/ _____/_____/ E-mail: inoue@greencross.co.jp From owner-7tms_r@net.bio.net Tue Jul 09 23:00:00 1996 Path: biosci!GREENCROSS.CO.JP!inoue From: inoue@GREENCROSS.CO.JP (Yoshihisa Inoue) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Model list Part.4 Date: 10 Jul 1996 03:35:38 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 64 Sender: daemon@net.bio.net Distribution: world Message-ID: <199607101036.TAA00916@greencross.co.jp> NNTP-Posting-Host: net.bio.net Dear netters, Hello again. I forgot to post the 3D model lists since Jan. in 1995. Followings are 1994, 1995, and 1996 lists. If you know other reports, please let me know. Thank you in advance. Part.4 is the 1994 version. **************************************************************** AU S.Watson and S.Arkinstall TI The G-Protein Linked Receptor -Facts Book- So Facts Book Series Com published by Academic Press, 427 page. (1994). ************************************************************************** AU C.Hutchins TI Three-dimensional Models of the D1 and D2 Dopamine Receptors SO Endocrine J., 2,7-23 (1994). AU R.Sankararamakrishnan, and M.S.P.Sansom TI Kinked Structures of Isolated Nicotinic Receptor M2 Helices: A Molecular Dynamics Study SO Biopolymers, 34, 1647-1657 (1994) Com each of the M2 helix is shown AU F.Jaehnig, O.Edholm, and J.Pleiss TI Structure and Dynamics of Membrane Proteins SO "Statistical Mechanics, Protein Structure, and Protein Substrate Interactions", Ed.by S.Doniach, pp229-237, Plenum Press, NY, 1994 Com bacteriorhodopsin dynamics AU M.Rodbell TI G-Proteins and Membrane Transduction: A New Model SO Int.Symp.Vasoact.Intest.Pept., Pituitary Adenylate Cyclase Act. Polypept. Relat.... , 101-112 (1994). Com No TM models, but G-protein mechanisms. AU I.Fujii, H.Nakamura, T.Sagara, and K.Kanematsu TI Molecular Model Building of Multiple Opioid Receptor Subtypes SO Med.Chem.Res.,4,424-431 (1994) AU P.Baldi, and Y.Chauvin TI Statistical Models of Proteins: An Application to the G-Protein-coupled Receptor Family SO Adv.Mol.Bioinf., Ed. by S.Schulze-kremer,pp53-102 (1994). IOS Press, Amsterdam, Neth. Com Chem.Abstr.,123,50118y AU X.Luo, D.Zhang, and H.Weinstein TI Ligand-induced domain motion in the activation mechanism of a G-protein-coupled receptor SO Protein Eng.,7,1441-1448 (1994) ____/ ___/ ___/ Yoshihisa INOUE (^_^) the Green Cross Corp. / / / 2-25-1 Shodai-Ohtani,Hirakata,Osaka 573 JAPAN / _ / / / tel: +81-720-56-9328 / / / / fax: +81-720-68-9597 _____/ _____/_____/ E-mail: inoue@greencross.co.jp From owner-7tms_r@net.bio.net Wed Jul 10 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!swrinde!sdd.hp.com!nntp.coast.net!news-res.gsl.net!news.gsl.net!hunter.premier.net!uunet!in2.uu.net!world!coopnews.coop.net!boulder.earthnet.net!usenet From: "Phillip E. Schwartz" Newsgroups: bionet.molbio.proteins.7tms_r Subject: New FDA Regs/Antibodies Date: Thu, 11 Jul 1996 12:46:37 -0700 Organization: Affinity BioReagents, Inc. Lines: 42 Message-ID: <31E55A1D.36C5@bioreagents.com> NNTP-Posting-Host: slip12.earthnet.net Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 2.0 (Win16; I) Please help! Will the individual at your organization who is in charge of regulatory affairs or who is concerned about the negative effects new FDA regulations may have on basic scientific and medical research in addition to health care in general please contact me regarding this matter. I am trying to get as many people to comment on and become involved in a grass-roots effort to keep the FDA from including all antibody research reagents as ‘in vitro diagnostic devices’. Granted, some are but most are not. Please review the information at: http:/www.earthnet.net/~affinity/fda/ It would be optimal if this website could be hotlinked to yours to gain the maximum amount of exposure prior to August 30, 1996 public comment deadline. I would appreciate any comments, questions, referred contacts, etc. that may be useful in changing the language of this pending FDA regulation. FDA Regulation: The Immunohistochemistry Reagents and Kits Regulation: 21/CFR 864 Docket Number: 94P-0342 Sincerely, Phillip E. Schwartz Antibody Partnership Project Manager Affinity BioReagents, Inc. 14818 W. 6th Ave., Suite 13A Golden, CO 80401 TEL: 800-527-4535 FAX: 303-278-2424 email: schwartz@bioreagents.com website: http://www.bioreagents.com/affinity From owner-7tms_r@net.bio.net Thu Jul 11 23:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.proteins.7tms_r Subject: IMPORTANT - BIOSCI Fundraising Update! Date: 12 Jul 1996 02:00:07 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 154 Sender: daemon@net.bio.net Distribution: world Message-ID: <199607120900.CAA09002@net.bio.net> NNTP-Posting-Host: net.bio.net BIOSCI is about halfway to its funding goal!! I'm interrupting the usual monthly posting of the BIOSCI miniFAQ to bring you up to date on BIOSCI fundraising progress, a topic of concern to your future use of this resource. Thank you in advance for taking the time to read this message carefully. Last year we announced that BIOSCI was going to adopt the U.S. Public Broadcasting System model to fund its operations after our DOE/NSF grant runs out later this year. Unlike PBS, we are not soliciting contributions from users; we are only selling ads on our Web pages solely to cover our operating costs. Our goal is to seek sponsorships until we build up an operating reserve of about $100,000 and then cease further promotions until we need to build the reserve back up. (The accountants among our readership will be familiar with the problem of deferred revenue which we can not safely utilize until ads have been displayed for a period of time.) We are only about halfway to our funding goal and need to raise further funds to avoid having to curtail services at net.bio.net. Fundraising is time-consuming, however, and we need your help as explained further below. Our operating costs consist of our network connection, phone lines, hardware maintenance (we will be getting newer and faster hardware soon!), plus 0.7 FTE of salaries covering UNIX systems admin, technical support, quality assurance, i.e., testing, of our system, and administrative costs (such as the time it takes to actually find/write/call potential sponsors and raise money!). Although the BIOSCI staff does get compensated for a portion of the work that they do, this project has always received a lot of free after-hours and "vacation" time labor, so we hope that no one will begrudge the time that we do charge to the project to serve you. All of the three part-time staff members, Dave Mack, Julie Lawrence, and myself, have full time day jobs and families in addition to working hard to keep this service running for all of you. Julie and Dave Mack are subcontractors for BIOSCI; my time that is charged to the project defrays a portion of my regular salary instead of adding to my income. Besides having to relocate the project, we were very busy this last year building new infrastructure such as our WWW hypermail interface to the system. This was released last December along with scores of WAIS indices for the newsgroups. Virtually everything is complete, although we do continue to find and fix bugs (many through your helpful feedback!). We are still having some problems with our WAIS indexing. The archives continue to grow rapidly. We are running over 100 indexes now versus three previously and any systems crashes cause greater havoc with the indexing than before! We are still working to fix this as fast as our resources permit and appreciate your patience, but we have been able to automate a lot of the infrastructure to reduce labor as compared to past requirements. We have also implemented new software to make moderation of BIOSCI/bionet newsgroups much easier and combat the growing problem of Internet junk mail and USENET "spamming." About 20% of our groups are now moderated, many of them by the BIOSCI staff! This, for example, made a major difference last year in the quality of content in our EMPLOYMENT/bionet.jobs.offered newsgroup which many commercial concerns and recruiting firms are using **without charge** to recruit candidates for positions in the biological sciences. We are also now in a position to have sponsors for individual newsgroups as you will have noticed if you have visited http://www.bio.net/ and clicked on "Access the BIOSCI/bionet newsgroups" recently. So, how can you help?? ---------------------- As noted above it can take a lot of time to contact potential sponsors if I have to do it all myself. Our request is quite simple. You can do two important things which will take very little time for you individually. First, please use our WWW system at http://www.bio.net/ to access the archives. You can now post or reply to messages via your Web browser. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. Our hope is to quickly raise several large corporate/institutional sponsors on our heavily-used WWW locations (some stats appended below), and then end this sponsorship campaign so that our resources can continue to be used for service provision, not fundraising. Many of our specialty newsgroup WWW archives are still used by small communities of scientists (and they haven't been heavily promoted yet). While these may be valuable niche markets to some advertisers, it will generate more labor and overhead having to find these sponsors, fairly price the locations, and deal with lots of smaller sponsorships than fewer mid-to large sponsors. We are striving to keep our operation as lean and efficient as possible since we are not trying to make careers out of running BIOSCI. We are trying if at all possible to avoid the administrative overhead entailed with processing lots of small payments to reach our fundraising goals. I'd like to thank all of you for your help in advance. In helping us, you are also helping yourselves, not only in keeping this resource available for all of the both large and small research communities that we serve, but also by alleviating the need for us to go back and compete with researchers for tight grant dollars! We promised NSF when we were awarded the BIOSCI grant that we would carry out this mission to make the service self-supporting. With your help, we will succeed in continuing BIOSCI's work into its second decade. Thank you very much! Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net A list of our prime WWW sponsorship locations follow. Please contact us for further details. ---------------------------------------------------------------------- The overall BIOSCI WWW pages are currently visited by users from close to 5500 unique computer hosts per week. Web servers only log the Internet computer/host name and frequently more than one individual can connect to us from a particular host. Main home page, http://www.bio.net, visited recently by about 2100 unique hosts per week Main Newsgroups archives page, http://www.bio.net/archives.html, visited recently by about 1200 Unique hosts per week BIO-JOURNALS archive page, http://www.bio.net/BIO-JOURNALS.html, visited recently by about 1000 unique hosts per week. EMPLOYMENT archive pages: http://www.bio.net:80/hypermail/EMPLOYMENT/ and monthly header pages, visited recently by about 800 unique hosts per week. Address database search page, http://www.bio.net/addrsearch.html, visited recently by about 450 unique hosts per week. Methods newsgroup archive pages, http://www.bio.net:80/hypermail/METHDS- REAGNTS/ and monthly header pages, visited recently by about 350 unique hosts per week. Ads can also be displayed on various combinations of other BIOSCI/bionet newsgroups. Please contact us at biosci-help@net.bio.net for details. ---------------------------------------------------------------------- From owner-7tms_r@net.bio.net Thu Jul 11 23:00:00 1996 Path: biosci!GREENCROSS.CO.JP!inoue From: inoue@GREENCROSS.CO.JP (Yoshihisa Inoue) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: model list part.5 Date: 12 Jul 1996 04:49:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 30 Sender: daemon@net.bio.net Distribution: world Message-ID: <960712205009.M0113250@gccss2.greencross.co.jp> References: <199607101037.TAA00934@greencross.co.jp> NNTP-Posting-Host: net.bio.net Dear netters, I am very much sorry. There are two parts of the lists I sent. The first part is the useful informations for helix-model. The latter is the model lists. >AU W.L.DeLano and A.Brunger >TI Helix packing in Proteins: Prediction and energetic analysis of > dimeric, trimeric, and tetrameric GCN4 coiled coil structures The Source reference is missing. But this is not the model list. I think the following references are more useful. AU J.W.Bryson, S.F.Betz, H.S.Lu, D.J.SUich, H.X.Zhou, K.T.O'Neil, W.F.DeGrado TI Protein Design: A Hierarchic Approach SO Science, 270, 935-941 (1995) AU R.Sankararamakrishnan, M.S.P.Sansom TI Modelling packing interactions in parallel helix bundles: Pentameric bundles of nicotinic receptor M2 Helices. SO Biochimica Biophysica Acta, 1239, 122-132 (1995). ____/ ___/ ___/ Yoshihisa INOUE (^_^) the Green Cross Corp. / / / 2-25-1 Shodai-Ohtani,Hirakata,Osaka 573 JAPAN / _ / / / tel: +81-720-56-9328 / / / / fax: +81-720-68-9597 _____/ _____/_____/ E-mail: inoue@greencross.co.jp From owner-7tms_r@net.bio.net Thu Jul 11 23:00:00 1996 Path: biosci!HLSUN.RED-CROSS.ORG!hlatim From: hlatim@HLSUN.RED-CROSS.ORG ("Timothy Hla") Newsgroups: bionet.molbio.proteins.7tms_r Subject: seeking BMP-2 Date: 12 Jul 1996 10:37:59 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: <9607121730.AA06753@hlsun.red-cross.org> Reply-To: "Timothy Hla" NNTP-Posting-Host: net.bio.net Dear Netters: I am urgently looking for a source of recombinant Bone Morphogenetic Protein (BMP)-2. Does anyone have any leads ? Thanks in Advance Timothy Hla, PhD Department of Molecular Biology Holland Laboratory, American Red Cross 15601 Crabbs Branch Way Rockville, MD 20855 ph: 301-738-0567 e-mail : hlatim@hlsun.red-cross.org From owner-7tms_r@net.bio.net Sun Jul 14 23:00:00 1996 Path: biosci!mail.nrgn.com!rbrodbeck From: rbrodbeck@mail.nrgn.com ("Robbin Brodbeck") Newsgroups: bionet.molbio.proteins.7tms_r Subject: G-Protein Localization. Date: 15 Jul 1996 08:42:55 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: <199607151542.IAA27038@net.bio.net> NNTP-Posting-Host: net.bio.net G-Protein Localization. Dear GPCR lovers: In the interest of better understanding the artificial expression systems we all use to express our various GPCR's, i.e., CHO, Sf9, etc., I am wondering about G-protein expression. There has been numerous postings in the last month or so dealing with the endogenous receptors expressed in most of the commen cell lines used in research, but nothing with respect to the endogenous GPs found! This also turns out to be a very important issue to us all! I've seen some older information, but I'm wondering if there is any recent data on the characterization of GP content for not only commen research cell lines, but also characterization of various tissues in the body with particular attention to regions of the brain. Thanks in advance, Robb From owner-7tms_r@net.bio.net Mon Jul 15 23:00:00 1996 Path: biosci!CITRUS.UCR.EDU!huangz From: huangz@CITRUS.UCR.EDU (John Huang) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Help needed about western Date: 16 Jul 1996 10:32:10 -0700 Organization: University of California Riverside Lines: 47 Sender: daemon@net.bio.net Distribution: world Message-ID: <199607161732.KAA17132@net.bio.net> NNTP-Posting-Host: net.bio.net Hi, Thanks for looking at my message. I got a problem about my western blot: My protein(called N300) is small(10 kDa calculated, 13 kDa and 20 kDa on 15% SDS-PAGE) and I expressed it as a GST fusion protein. After harvesting the GST-N300 fusion protein, I cleave it with thrombin and run 15% SDS-PAGE to separate GST protein, N300 protein and uncleaved GST-N300 fusion protein. Then I transfer the whole lane from SDS-PAGE gel onto membrane( nitrocellulose or PVDF). After transfer, I block the membrane with 10% milk in TTBS-> wash with TTBS -> incubate with first antibody(anti-N300) in TTBS -> wash with TTBS -> incubate with second antibody ->wash with TTBS -> ECL reaction -> expose X-ray film. After the whole western procedure, I stained the membrane and found N300 band disappeared totally. No band showed on film corresponding to N300 protein. I did some trouble shooting: 1. Transfer is successful(After transfer, I stained membrane and saw very good bands for N300 protein). 2. Use of Tween-20 in Western blot wash off N300 from membrane strongly(both bands were washed off in 0.1% Tween-20 TBS in 15 minutes). But shaking in TBS overnight did not make N300 bands fall off from membrane. 3. UV corsslinked NC membrane cannot keep N300 on membrane for Western. 4. Both 0.2 um and 0.45 um pore size nitrocellulose and PVDF membrane do notwork well to keep N300 on membrane for Western. 5. Not use Tween-20 and shorten antibody incubation time to 30 minutes for each antibody can keep up to 10% N300 band left on membrane, but the Western film showed strong background and make the true N300 band on film very hard to see. 6. I use the recombinant protein, therefore the amount of protein N300 is not a problem. Now I am going to try using gelatin or BSA to block membrane. If you happen to know or get some experience about Western blot of very small protein(such as 10 kDa), would you give me some suggestion? My email address: huangz@citrus.ucr.edu I shall very much appreciate your assistance. John Huang on 7/16/96 From owner-7tms_r@net.bio.net Tue Jul 16 23:00:00 1996 Newsgroups: bionet.molbio.proteins.7tms_r Path: biosci!rutgers!uwm.edu!news-res.gsl.net!news.gsl.net!usenet.eel.ufl.edu!warwick!bsmail!usenet From: "Dr Andrew J. Doherty" Subject: Re: Help needed about western Content-Type: text/plain; charset=us-ascii To: John Huang Message-ID: <31EC9AA5.65A3@bsa.bristol.ac.uk> Sender: usenet@uns.bris.ac.uk (Usenet news owner) Nntp-Posting-Host: anaajd.ana.bris.ac.uk Content-Transfer-Encoding: 7bit Organization: University of Bristol, UK References: <199607161732.KAA17132@net.bio.net> Mime-Version: 1.0 Date: Wed, 17 Jul 1996 07:47:49 GMT X-Mailer: Mozilla 3.0b5a (Win16; I) Lines: 79 John Huang wrote: > > Hi, Thanks for looking at my message. > > I got a problem about my western blot: My protein(called N300) is > small(10 kDa calculated, 13 kDa and 20 kDa on 15% SDS-PAGE) and I > expressed it as a GST fusion protein. After harvesting the GST-N300 > fusion protein, I cleave it with thrombin and run 15% SDS-PAGE to > separate GST protein, N300 protein and uncleaved GST-N300 fusion protein. > Then I transfer the whole lane from SDS-PAGE gel onto membrane( > nitrocellulose or PVDF). After transfer, I block the membrane with 10% > milk in TTBS-> wash with TTBS -> incubate with first antibody(anti-N300) > in TTBS -> wash with TTBS -> incubate with second antibody ->wash with > TTBS -> ECL reaction -> expose X-ray film. > > After the whole western procedure, I stained the membrane and found N300 > band disappeared totally. No band showed on film corresponding to N300 > protein. > > I did some trouble shooting: > 1. Transfer is successful(After transfer, I stained membrane and saw very > good bands for N300 protein). > 2. Use of Tween-20 in Western blot wash off N300 from membrane > strongly(both bands were washed off in 0.1% Tween-20 TBS in 15 minutes). > But shaking in TBS overnight did not make N300 bands fall off from > membrane. > 3. UV corsslinked NC membrane cannot keep N300 on membrane for Western. > 4. Both 0.2 um and 0.45 um pore size nitrocellulose and PVDF membrane do > notwork well to keep N300 on membrane for Western. > 5. Not use Tween-20 and shorten antibody incubation time to 30 minutes > for each antibody can keep up to 10% N300 band left on membrane, but the > Western film showed strong background and make the true N300 band on film > very hard to see. > 6. I use the recombinant protein, therefore the amount of protein N300 is > not a problem. > > Now I am going to try using gelatin or BSA to block membrane. > > If you happen to know or get some experience about Western blot of very > small protein(such as 10 kDa), would you give me some suggestion? > > My email address: huangz@citrus.ucr.edu > > I shall very much appreciate your assistance. > > John Huang on 7/16/96 One of the first questions is, what primary antibody are you using? Is it one which has already been characterised as working well in westerns? Is it a polyclonal serum, affinity-purified antibody or monoclonal? If you are getting very high background with ECL, I would imagine that it is a polyclonal serum, in which case you will need to purify the antibody. ECL is a very sensitive detection technique and normal sera are too dirty to use with it (in my experience at least). I must admit that I've never worked with proteins that are as small as 10K, but there shouldn't be a problem with keeping the protein actually on the NC/PVDF once it's been transferred. Do you dry the PVDF after transfer? This may help in locking the protein into the membrane although the reactivity of the antibody may then be impaired. You could also try and transfer the GST-N300 fusion and use a commercial anti-GST antibody.This would at least let you determine conditions under which the whole procedure could work and then to try it with the N300 antibody. Maybe you could get away with not cleaving the fusion product at all. Hope this helps, and good luck -- *********************************************** Dr Andrew Doherty Department of Anatomy School of Medical Sciences University Walk Bristol UK BS8 1TD e-mail Doherty@bsa.bristol.ac.uk ************************************************ From owner-7tms_r@net.bio.net Tue Jul 16 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!harbinger.cc.monash.edu.au!nntp.coast.net!news-res.gsl.net!news.gsl.net!usenet.eel.ufl.edu!news.ultranet.com!usenet From: "John P. McGrath" Newsgroups: bionet.molbio.proteins.7tms_r Subject: BKR antibodies Date: Wed, 17 Jul 1996 10:11:31 -0400 Organization: UltraNet Communications, Inc. Lines: 13 Message-ID: <31ECF493.530@ma.ultranet.com> Reply-To: Cambridge, MA NNTP-Posting-Host: d104.wal.ma.ultra.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) I am in search of good antibodies, polyclonal or monoclonal, that recognize the rat bradykinin B2 receptor. They will be used primarily for immunocytochemistry and Western blots. Any info would be greatly appreciated. Thanks, J.P. McGrath Alkermes, Inc. 64 Sidney Street Cambridge, MA 02139 T: (617) 494-0171 F: (617) 494-9263 E: bigred@ma.ultranet.com From owner-7tms_r@net.bio.net Wed Jul 17 23:00:00 1996 Path: biosci!NEOCORTEX.HEALTH.UFL.EDU!shaw From: shaw@NEOCORTEX.HEALTH.UFL.EDU ("Gerry Shaw") Newsgroups: bionet.molbio.proteins.7tms_r Subject: 7Tms receptor sequence alignment Date: 18 Jul 1996 14:00:33 -0700 Organization: UF Department of Neurosurgery Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: <21C93E76BC4@NEOCORTEX.HEALTH.UFL.EDU> NNTP-Posting-Host: net.bio.net Dear 7tmers, Is a downloadable sequence alignment for a variety of different 7tmr available anywhere? If so where? Thanx, Yours, Gerry Shaw University of Florida College of Medicine Department of Neuroscience JHMHC Box J-100244 Gainesville Florida 32610 Tel (904) 392 9446 Fax (904) 392 8347 http://www.ufbi.ufl.edu/people/shaw/shawhp.htm "Life is an experiment without a control" From owner-7tms_r@net.bio.net Thu Jul 18 23:00:00 1996 Path: biosci!EMBL-HEIDELBERG.DE!Gert.Vriend From: Gert.Vriend@EMBL-HEIDELBERG.DE Newsgroups: bionet.molbio.proteins.7tms_r Subject: addition Date: 19 Jul 1996 12:37:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <199607191936.VAA01636@nu> NNTP-Posting-Host: net.bio.net Please neglect the message about the multali directory in the GPCRDB database. This database got updated about a week ago. We did not want to announce this yet because we are still testing, but the main pointer now should be : http://swift.embl-heidelberg.de/7tm/ and not directly to any of the subpages such as multali. The multali directory no longer exists. Alignments have been combined with the sequences. There now also are several 'overall' alignments (for what they are worth...). Gert. From owner-7tms_r@net.bio.net Thu Jul 18 23:00:00 1996 Path: biosci!agate!spool.mu.edu!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!fu-berlin.de!news.belwue.de!news.uni-hohenheim.de!wpxx02!not-for-mail From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: 7Tms receptor sequence alignment Date: 19 Jul 1996 14:36:48 GMT Organization: CC University of Hohenheim (not responsible for contents) Lines: 15 Distribution: world Message-ID: <4so6i0$1i3q@power5.rz.uni-hohenheim.de> References: <21C93E76BC4@NEOCORTEX.HEALTH.UFL.EDU> NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Gerry Shaw (shaw@NEOCORTEX.HEALTH.UFL.EDU) wrote: : Is a downloadable sequence alignment for a variety of different 7tmr : available anywhere? If so where? http://www.sander.embl-heidelberg.de/7tm/multali/multali.html contains a couple of multiple alignments for various families but not a single alignment for all receptors. --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP3 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-7tms_r@net.bio.net Thu Jul 18 23:00:00 1996 Path: biosci!bms.com!watson_j From: watson_j@bms.com (John Watson) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: 7Tms receptor sequence alignment Date: 19 Jul 1996 06:52:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 41 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net >Is a downloadable sequence alignment for a variety of different 7tmr >available anywhere? If so where? Thanx, > >Yours, > >Gerry Shaw >University of Florida College of Medicine >Department of Neuroscience >JHMHC Box J-100244 >Gainesville >Florida 32610 >Tel (904) 392 9446 >Fax (904) 392 8347 >http://www.ufbi.ufl.edu/people/shaw/shawhp.htm Try http://swift.embl-heidelberg.de/7tm/ http://receptor.mgh.harvard.edu/GCRDBHOME.html http://mgddk1.niddk.nih.gov:8000/MRS.html http://www-grap.fagmed.uit.no/GRAP/homepage.html/ All the best, AJW ------------------------------------------------------------------------ __ __ __ A. John Watson, Ph.D. /__/__/__/\ Bristol-Myers Squibb Company /__/__/__/\/\ Pharmaceutical Reseach Institute /__/__/__/\/\/\ CNS Molecular Biology, Dept. 405 \__\__\__\/\/\/ 5 Research Parkway \__\__\__\/\/ Wallingford, CT 06492 \__\__\__\/ watson_j@bms.com | 203.284.6745 voice | Standard Disclaimers Apply 203.284.7569 fax | ------------------------------------------------------------------------ From owner-7tms_r@net.bio.net Sun Jul 21 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!spool.mu.edu!uwm.edu!newsfeed.internetmci.com!newsxfer2.itd.umich.edu!portc01.blue.aol.com!news-res.gsl.net!news.gsl.net!usenet.eel.ufl.edu!warwick!news.coventry.ac.uk!leofric!psandhu From: PERDEEP SANDHU Newsgroups: bionet.molbio.proteins.7tms_r Subject: PROLYL-4-HYDROXYLASE Date: Mon, 22 Jul 1996 10:20:25 +0100 Organization: Coventry University Lines: 9 Message-ID: NNTP-Posting-Host: leofric-fddi.coventry.ac.uk Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Sender: psandhu@leofric Hi, I was wondering if anyone could help me. I am undergoing a training placement for a year before I start my third year of a biology degree course. My project is based upon the enzyme prolyl-4-hydroxylase which is compsed of an alpha and bets subunit and is responsible for the hydroxylation of peptidyl proline residues. I would be greatful for any information sent o me at the above address. Thanks. From owner-7tms_r@net.bio.net Mon Jul 22 23:00:00 1996 Path: biosci!RUBENS.ITS.UNIMELB.EDU.AU!pms From: pms@RUBENS.ITS.UNIMELB.EDU.AU (Patrick Sexton) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Functional responses in 5-HT2c/293 Date: 23 Jul 1996 16:28:55 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 47 Sender: daemon@net.bio.net Distribution: world Message-ID: <01I7G2QM1NPE8WZK0Y@rubens.its.unimelb.edu.au> NNTP-Posting-Host: net.bio.net At 01:31 PM 23-07-96 +0000, Dave Thomas wrote: > >Hi, > I have noted a gradual rise in basal PI turnover combined with reduced agonist stimulation in cloned human 5-HT2C/293 cells. This seems to be related to cell passage number. The cells are grown in serum containing medium but are switched to 1% dialsyed serum medium (around 10nM 5-HT) during 3H-inositol labelling and then serum-free medium for 24 hours prior to assay. Therefore endogenous 5-HT should not be a problem (?). The effect seen might therefore be due to an increase in constitutive coupling. I'd be grateful for comments! >Cheers, > Dave :) We have seen a similar phenomenon in HEK-293 cells stably transfected with the rat C1b calcitonin receptor. In our hands there is a rightward shift in the dose-response curve to calcitonin with increasing passage number - the shift being up to 2 orders of magnitude going from passage 30 to 90. These cells express a high level of receptors (~6,000,000 receptors/cell) and exhibit constitutive activity with respect to cAMP generation (which is the parameter we are measuring). These cells also show increased basal cAMP levels with respect to time in passage and the fold-increase in response to agonists is also greater with increased passage number. The basal activity of the system is reversed by "inverse agonists" but not by a "neutral antagonist" for calcitonin receptors, indicating, that at least for our system, that the increase in basal activity is due to constitutively active receptors. It is reasonably well documented that constitutively active receptors can exhibit constitutive desensitisation, presumably associated with the action of GRKs, and we believe that this is what occurs in our system. The increase in basal response (and the greater fold-increase) in later passages we believe is due to up regulation of down stream (of the receptor) components of the signalling cascade, potentially the G-proteins. I hope these comments are of some use. Regards, Patrick ---------------------------------------------------------------------------- ------------- Patrick M. Sexton, PhD St. Vincent's Institute of Medical Research 41 Victoria Parade, Fitzroy 3065, Victoria, AUSTRALIA Phone: 61-3-9288-2480 Fax: 61-3-9416-2676 E-mail: pms@rubens.its.unimelb.edu.au From owner-7tms_r@net.bio.net Mon Jul 22 23:00:00 1996 Path: biosci!agate!spool.mu.edu!uwm.edu!newsfeed.internetmci.com!newsserver.jvnc.net!netnews.sbphrd.com!ham180.ha.uk.sbphrd.com!user From: Dave_Thomas-1@sbphrd.com (Dave Thomas) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Functional responses in 5-HT2c/293 Date: 23 Jul 1996 13:31:23 GMT Organization: SmithKline Beecham Lines: 8 Message-ID: Reply-To: Dave_Thomas-1@sbphrd.com NNTP-Posting-Host: ham180.ha.uk.sbphrd.com X-Newsreader: Value-Added NewsWatcher 2.0b22.0+ Hi, I have noted a gradual rise in basal PI turnover combined with reduced agonist stimulation in cloned human 5-HT2C/293 cells. This seems to be related to cell passage number. The cells are grown in serum containing medium but are switched to 1% dialsyed serum medium (around 10nM 5-HT) during 3H-inositol labelling and then serum-free medium for 24 hours prior to assay. Therefore endogenous 5-HT should not be a problem (?). The effect seen might therefore be due to an increase in constitutive coupling. I'd be grateful for comments! Cheers, Dave :) -- The opinions expressed in this communication are my own, and do not necessarily reflect those of my employer. From owner-7tms_r@net.bio.net Mon Jul 22 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Alexy Eroshkin Newsgroups: bionet.molbio.proteins.7tms_r Subject: ANNOUNCEMENT: Protein Multiple Sequences Editor for Win 3.x/95 Date: 23 Jul 1996 12:02:27 +0100 Organization: State Research Center of Virology & Biotechnology VECTOR Lines: 46 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4t2bg3$qne@mserv1.dl.ac.uk> Original-To: 7tms_r@dl.ac.uk To: 7tms_r@dl.ac.uk From: eroshkin@vector.nsk.su Subject: ANNOUNCEMENT: Protein Multiple Sequences Editor for Win 3.x/95 Dear All, With the increasing of protein sequence data, multiple alignment is one of the important techniques to understand protein structure-function organization and evolution. New software package ProMSED, Protein Multiple Sequences EDitor for Windows 3.x/95, is available from EBI software library: ftp://ftp.ebi.ac.uk/pub/software/dos/promsed/ (as self-extracted archive). ProMSED is an easy-to-use application for Windows 3.x/95 that performs automatic and manual multiple protein sequences alignment, alignment analysis and editing. The program reads NBRF/PIR, Pearson (Fasta), EMBL/SwissProt, Intelligenetics and CLUSTAL data formats and has interface and the main functions similar to popular text editor Word. ProMSED loads up to eight protein families in time, adds sequences to existing alignment, combines sequences from different files, outputs the alignment in two formats, prints the alignment. Automatic alignment is based on ClustalV algorithm. Manual alignment and sequence analysis are facilitated by using group operations and amino acid color-coding reflecting amino acid similarity in physico-chemical and mutational properties, secondary structure propensities, etc. ProMSED can align complete set of sequences, its subset and any selected block, providing thus flexible tool for sequences analysis, visualization, edition and illustrations preparation. A HELP is included. Demo version has limits on length and number of sequences. Special thanks to Dr. Desmond Higgins for source code of ClustalV. Comments, bug reports and suggestions for new features are welcome and should be sent by email to eroshkin@vector.nsk.su. Inquiries can be addressed to: ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Dr. Alexey Eroshkin Institute of Molecular Biology E.mail: eroshkin@vector.nsk.su State Research Center of Virology and Tel: +7 (3832) - 647774 Biotechnology "Vector" Fax: +7 (3832) - 328831 Koltsovo, Novosibirsk Region 633159 Russia ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ From owner-7tms_r@net.bio.net Wed Jul 24 23:00:00 1996 Path: biosci!WH.BAYER.COM!huy From: huy@WH.BAYER.COM (Yinghe Hu) Newsgroups: bionet.molbio.proteins.7tms_r Subject: postdoc. position Date: 25 Jul 1996 07:17:19 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <199607251410.AA28703@miwr03il_e.wh.bayer.com> NNTP-Posting-Host: net.bio.net A postdoctoral position is available to study the function of a G-protein coupled neuropeptide Y receptor in the hypothalamus. Research will involve the functional studies of the receptor on the feeding behavior and obesity. Research background in molecular neurobiology is required; some expreience with in situ hybridization and immunohistochemistry is desirable. Please contact: Dr. Yinghe Hu Bayer Corp. 400 Morgan Lane, B24 West Haven, CT 06516 Email: huy@wh.bayer.com Telephone: 203-931-5062 FAX: 203-937-2686 From owner-7tms_r@net.bio.net Wed Jul 31 23:00:00 1996 Path: biosci!MSMAIL.BMS.COM!Carlson_Kenneth_E.PRILVMS3 From: Carlson_Kenneth_E.PRILVMS3@MSMAIL.BMS.COM (Carlson Kenneth E) Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe Date: 1 Aug 1996 15:40:11 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net From owner-7tms_r@net.bio.net Wed Jul 31 23:00:00 1996 Path: biosci!MSMAIL.BMS.COM!Carlson_Kenneth_E.PRILVMS3 From: Carlson_Kenneth_E.PRILVMS3@MSMAIL.BMS.COM (Carlson Kenneth E) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 1 Aug 1996 15:40:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net subscribe 7tms_r