From owner-7tms_r@net.bio.net Mon Feb 03 22:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!rill.news.pipex.net!pipex!oleane!pasteur.fr!jussieu.fr!univ-angers.fr!ciril.fr!not-for-mail From: Fabien Campagne Newsgroups: bionet.molbio.proteins.7tms_r Subject: Viseur 3.0 interacts with TinyGRAP Date: Tue, 04 Feb 1997 19:10:13 +0000 Organization: Laboratoire de Chimie The'orique de Nancy Lines: 28 Message-ID: <32F78995.52BF@incm.u-nancy.fr> NNTP-Posting-Host: yellow.incm.u-nancy.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0SC-SGI (X11; I; IRIX64 6.2 IP28) Viseur 3.00 is now released. This new version have the following new features: + TinyGRAP interaction: Viseur can now fetch mutations directly from TinyGRAP and display them on the usual views (sequence, snake-like, alignment, 3D model). + ModelView: A solid ribbon can be displayed. Thanks to PDB2VRML. + A reorganized and extended Painting module (used to display TinyGRAP mutant information). and contains some bug fixes. For more information about the program features, program distribution, and more, please visit: http://brown.incm.u-nancy.fr/viseur/viseur.html (Viseur Home page) http://brown.incm.u-nancy.fr/viseur/using/documentation/main.html (Documentation main page) http://brown.incm.u-nancy.fr/viseur/snake-like-view/OPRD_HUMAN.html (snake-like clickable HTML output illustration) http://brown.incm.u-nancy.fr/viseur/BACTER_RHODO.wrl.gz (3DModel VRML output illustration) http://brown.incm.u-nancy.fr/viseur/using/documentation/ViseurTinyGRAP.html (TinyGRAP mutants visualization) thanks for your attention, Fabien Campagne -- campagne@incm.u-nancy.fr | Lab. de Chimie Theorique phone: (033) 83 91 20 00 extension 3236 | Nancy, France. From owner-7tms_r@net.bio.net Tue Feb 04 22:00:00 1997 Path: biosci!cochin.inserm.fr!gros From: gros@cochin.inserm.fr (Jerome Gros) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Adenylyl Cyclase subtypes Date: 5 Feb 1997 00:40:57 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hello I was wonder if anyone has information (references etc) on the expression of adenylyl cyclase isoforms in CHO cells and HEK293 cells. Jerome ____________________________________________________________________________ Jerome GROS ICGM - UPR0415 CNRS email : gros@cochin.inserm.fr *********** 22, rue Mechain Tel (33) 01 40 51 64 22/21 * BioDocs * F-75014 PARIS fax (33) 01 40 51 72 10 *********** ____________________________________________________________________________ From owner-7tms_r@net.bio.net Sat Feb 08 22:00:00 1997 Path: biosci!biosci!not-for-mail From: Arne Elofsson Newsgroups: bionet.molec-model,bionet.xtallography,bionet.molbio.proteins.7tms_r Subject: Postdoctoral position in Bioinformatics / Computational Biology. Date: 9 Feb 1997 13:58:10 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 99 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Xref: biosci bionet.molec-model:1363 bionet.xtallography:3182 bionet.molbio.proteins.7tms_r:1030 Postdoctoral position in Bioinformatics / Computational Biology. Analysis and prediction of membrane protein structures. Applicant are invited for a 2-year postdoctoral fellowship at the University of Stockholm, Department of Biochemistry in the groups of Professor Gunnar von Heijne and Associate Professor Arne Elofsson (This applications can also be found at http://www.biokemi.su.se/~arne/postdoc_vt97/ ) The Project The number of membrane proteins with known 3D structure has increased recently and a number of hypotheses about membrane protein structure can now be tested. The goal of the project is to analyze known 3D structures and derive rules that can be used for structure prediction of membrane proteins. These studies will be performed in close contact with experimental studies performed in the group of Gunnar von Heijne. The applicant We are looking for a person with a Ph.D in life science / computer scienc= e / bioinformatics with experience of working in an UNIX environment. Programming experience and experience of sequence analysis / molecular modeling / structural biology is desired. The group The biocomputing group presently consists of five people: three graduate students, one assistant professor (Arne Elofsson), and one full professor (Gunnar von Heijne). The group of Gunnar von Heijne uses both experimenta= l and theoretical techniques to study membrane protein assembly and structure. The group of Arne Elofsson also works on ab initio protein folding studies, threading / fold-assignments, and homology modeling. The computing environment is based on local UNIX workstations and we also have access to the Swedish National Supercomputer Center in Stockholm. We have close collaborations with several groups both in Sweden and abroad. Recent Papers "Local moves, an efficient method for protein folding simulations" Arne Elofsson, Scott Le Grand & David Eisenberg, 1995 Proteins, Structure Function and Genetics 23:73-82 Wallin, E., and von Heijne, G. (1995) Properties of N-terminal tails in G-protein coupled receptors - A statistical study. Protein Engineer. 8, 693-698. Nielsen, H., Engelbrecht, J., von Heijne, G., and Brunak, S. (1996) Defining a Homology Threshold for a Functional Protein Sequence Pattern: The Signal Peptide Cleavage Site. PROTEINS: Struct. Funct. Genet. 24, 165-177. Nielsen, H., Engelbrecht, J., Brunak, S. and von Heijne, G. (1997) A neur= al network method for identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Prot.Engineer., 10, 1-6. Recent advances in how to test knowledge based energy functions for prote= in folding studies." Arne Elofsson, 1997 in press in "Recent Research Developments in Physical Chemistry" "Prediction of transmembrane alpha-helices: Application of the Dense Alignment Surface method" Miklos Cserz=F6, Erik Wallin, Istvan Simon, Gun= nar von Heijne, Arne Elofsson, 1996 submitted. "Architecture of helix bundle membrane proteins. An analysis of Cytochrom= e c oxidase from bovine mitochondria.", Erik Wallin, Tomotake Tsukihara, Shinya Yoshikawa, Gunnar von Heijne, Arne Elofsson, 1997 in press in Protein Science For more information, please contact: Arne Elofsson (arne@biokemi.su.se; http://www.biokemi.su.se/~arne/) or Gunnar von Heijne (gunnar@biokemi.su.se; http://www.biokemi.su.se/~gvh/) --=20 ----------------------------------------------------------------- Arne Elofsson arne@bimbo.biokemi.su.se http://www.biokemi.su.se/~arn= e/ Tel:+46(0)8-161553 Dep, of Biochemistry, Stockholm University Fax:+46(0)8-153679 10691 Stockholm, Sweden From owner-7tms_r@net.bio.net Sun Feb 09 22:00:00 1997 Path: biosci!VIOLET.INCM.U-NANCY.FR!poda From: poda@VIOLET.INCM.U-NANCY.FR (Gennady PODA) Newsgroups: bionet.molbio.proteins.7tms_r Subject: 7tms: Chemokine Receptors in AIDS problem: a possible collaboration?.. Date: 10 Feb 1997 08:40:38 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 53 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net If you do not work on GPCRs mutagenesis and are not interested in AIDS problem please just discard this message. Dear Netters, We are looking for a collaboration with an experimental group(s) doing site-directed mutagenesis of GPCRs. We work on GPCRs modeling and our current interest is two chemokine receptors (CXCR-4/fusin and CC-CKR-5) which have been very recently found to be two main co-receptors for HIV envelop/host cells fusion. Currently, we have built a 3D model of CXCR-4 receptor and of its ligand, SDF-1, as well as for the ligand/receptor interaction. A 3D model of CKR-5 and docking proposals for its ligands (MIP-1alpha, MIP-1beta and RANTES) are hoped to come soon. In order to proceed we have used numerous mutagenesis data for Interleukin-8 alpha receptor as well as for IL8 itself. To day, the mutation data neither for those two receptors nor for their ligands (except some for RANTES) are not available, at least for us. So in this case the models can not be evaluated directly. But, being evaluated, they can be used to propose which residues should be substituted first according to the model. So I would be extremely grateful if someone(s) of you let me know at your earliest convenience if some kind of collaboration can be initiated between us if you do have some interest in modeling. The so called feed-back could be established between our computational and your experimental group(s). If not, I would be very thankful if you kindly provide me with any references or information on it. I will summarize your quotations. Thank you very much beforehand for your participation. I am looking forward to our possible fruitful for both side collaboration. Sincerely yours, Gennady Poda ************************************************************ Dr. Gennady PODA Laboratoire de Chimie theorique (UA 510 du C.N.R.S.) Universite Henri Poincare, Nancy-I Faculte des Sciences - Domaine scientifique Victor Grignard B.P. 239 - 54506 Vandoeuvre-les-Nancy Cedex France Fax : + 33 (0)3.83.91.25.30 E-mail : poda@incm.u-nancy.fr ************************************************************ From owner-7tms_r@net.bio.net Sun Feb 09 22:00:00 1997 Path: biosci!NEWSSUN.MED.MIAMI.EDU!vslepak From: vslepak@NEWSSUN.MED.MIAMI.EDU ("Vladlen Z. Slepak") Newsgroups: bionet.molbio.proteins.7tms_r Subject: 7TMRs in retina Date: 10 Feb 1997 08:01:25 -0800 Organization: University of Miami Medical School Lines: 6 Sender: daemon@net.bio.net Distribution: world Message-ID: <32FEFFDD.3F4B@newssun.med.miami.edu> Reply-To: vslepak@newssun.med.miami.edu NNTP-Posting-Host: net.bio.net Question: What is known about 7TMRs on the plasma membrane of Rod Outer Segments (ROS)? - other than Rhodopsin, of course. Vlad From owner-7tms_r@net.bio.net Sun Feb 09 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!www.nntp.primenet.com!nntp.primenet.com!arclight.uoregon.edu!news.ibm.net.il!news.biu.ac.il!news.tau.ac.il!usenet From: Daniel Chamovitz Newsgroups: bionet.molbio.proteins.7tms_r Subject: inclusion bodies Date: Mon, 10 Feb 1997 10:51:15 +0200 Organization: Tel Aviv University Lines: 7 Message-ID: <32FEE17F.30CF@post.tau.ac.il> Reply-To: chamd@post.tau.ac.il NNTP-Posting-Host: britanya407.tau.ac.il Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Macintosh; I; PPC) Hello, I am having troubles in solubilization of pET vector E coli inclusion bodies. I have tried 1% Tween 20, 8M urea, and 1M NaCl (in different combinations). If you have a better procedure please contact me. Thanks. Tally Yahalom dannych@life.tau.ac.il From owner-7tms_r@net.bio.net Mon Feb 10 22:00:00 1997 Path: biosci!UNLINFO.UNL.EDU!jfrank From: jfrank@UNLINFO.UNL.EDU (julie frank) Newsgroups: bionet.molbio.proteins.7tms_r Subject: unsubscribe Date: 11 Feb 1997 08:33:07 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <9702111633.AA22065@unlinfo.unl.edu> NNTP-Posting-Host: net.bio.net unsubscribe From owner-7tms_r@net.bio.net Mon Feb 10 22:00:00 1997 Path: biosci!navix.net!jf83824 From: jf83824@navix.net (Julie Frank) Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe Date: 11 Feb 1997 10:28:06 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <3300BB2B.6082@navix.net> NNTP-Posting-Host: net.bio.net subscribe From owner-7tms_r@net.bio.net Mon Feb 10 22:00:00 1997 Path: biosci!novo.dk!byw From: byw@novo.dk (Robert Bywater) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 11 Feb 1997 23:49:05 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <199702120748.IAA29664@bach.novo.dk> NNTP-Posting-Host: net.bio.net subscribe From owner-7tms_r@net.bio.net Tue Feb 11 22:00:00 1997 Path: biosci!FHS.CSU.MCMASTER.CA!groverak From: groverak@FHS.CSU.MCMASTER.CA ("A.K. Grover") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Electronic Journal Club Date: 12 Feb 1997 16:56:23 -0800 Organization: McMaster University Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: <3302A6A3.974@fhs.mcmaster.ca> Reply-To: groverak@fhs.csu.McMaster.CA NNTP-Posting-Host: net.bio.net New Electronic Journal Club in Molecular Pharmacology http://www-fhs.mcmaster.ca/mcpharm/ From owner-7tms_r@net.bio.net Tue Feb 11 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!csn!nntp-xfer-1.csn.net!csn!nntp-xfer-2.csn.net!tali.UCHSC.edu!chris From: David Port Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe Date: Wed, 12 Feb 1997 13:30:14 -0700 Organization: University of Colorado HSC Lines: 1 Message-ID: <33022856.55EA@uchsc.edu> Reply-To: david.port@uchsc.edu NNTP-Posting-Host: wire.uchsc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (Win95; I) subscribe 7TMS_R From owner-7tms_r@net.bio.net Tue Feb 11 22:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.proteins.7tms_r Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 12 Feb 1997 02:00:22 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199702121000.CAA18818@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-7tms_r@net.bio.net Wed Feb 12 22:00:00 1997 Path: biosci!VIOLET.INCM.U-NANCY.FR!poda From: poda@VIOLET.INCM.U-NANCY.FR (Gennady PODA) Newsgroups: bionet.molbio.proteins.7tms_r Subject: 7tms: Chemokine receptor mutagenesis study quotation Date: 13 Feb 1997 03:18:47 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 144 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Netters, I am very thankful to all people who have answered my annoncement in 7tms_r concerning a possible collaboration between theoretical and experimental group(s) and references about mutagenesis study of coreceptors important for HIV enrtance into the host cells:=20 =09- Mark Goldsmith at University of California, San Francisco=20 =09 (I contacted him before); =09- Jeffrey Harrison at University of Floria; =09- Mark Parmentier at IRIBHN, Universite libre de Bruxelles; =09- Joseph Rucker at University of Pennsylvania; Thanks for their references. It seems that single-point mutations of those coreceptors have not been investigated yet.=20 - Molecular cloning and functional characterization of a new CC-chemokine receptor gene. Samson M, Labb=E9 O, Mollereau C, Vassart G and Parmentier M= .=20 Biochemistry 35 (1996) 3362-3367.=20 - A dual-tropic, primary HIV-1 isolate that uses both fusin and the b-chemokine receptor CKR-5 as entry cofactors. Doranz BJ, Rucker J, Yi Y, Smyth RJ, Samson M, Parmentier M, Collman RG, and Doms RW. Cell 85 (1996) 1149-1158.=20 - Resistance to HIV-1 infection of Caucasian individuals bearing mutant alleles of the CCR5 chemokine receptor gene. Samson M, Libert F, Doranz BJ, Rucker J, Liesnard C, Farber CM, Saragosti S, Lapoum=E9roulie C, Cogniaux J, Forceille C, Muyldermans G, Verhofstede C, Guy Burtonboy G, Georges M, Imai T, Rana S, Yi Y, Smyth RJ, Collman RG, Doms RW, Vassart G and Parmentier M. Nature 382 (1996) 722-725.=20 - The genes encoding the human CC-chemokine receptors CC-CKR1 to 5 are clustered in the p21.3-p24 region of chromosome 3. Samson M, Soularue P, Vassart G and Parmentier M. Genomics 36 (1996) 522-526.=20 - Regions in b-Chemokine Receptors CCR5 and CCR2b that Determine HIV-1 Cofactor Specificity. Rucker J, Samson M, Doranz BJ, Libert F, Berson JF, Yi Y, Collman RG, Broder CC, Vassart G, Doms RW and Parmentier M. Cell 87 (1996) 437-446.=20 - Molecular cloning and chromosomal mapping of a novel human gene, ChemR1, expressed in CD4+ and CD8+ T lymphocytes and encoding a receptor related to chemokine receptors. Samson M, Stordeur P, Labb=E9 O, Soularue P, Vassar= t G and Parmentier M. Eur. J. Immunol. 26 (1996) 3021-3028.=20 - Role of CCR5 in infection of primary macrophages and lymphocytes by M-tropic strains of HIV: resistance to patient-derived and prototype isolates resulting from the Dccr5 mutation. Rana S, Besson G, Cook DG, Rucker J, Smyth RJ, Yi Y, Turner J, Guo HH, Du JG, Peiper SC, Lavi E, Samson M, Libert F, Liesnard C, Vassart G, Doms RW, Parmentier M and Collman RG. J. Virol. (1997) in press - Molecular cloning and functional characterization of a novel human CC chemokine receptor (CCR5) for RANTES, MIP-1beta, and MIP-1alpha. Raport CJ, Gosling J, Schweickart VL, Gray PW, Charo IF. J. Biol. Chem. 271 (1996) 17161-17166.=20 - Identification of two rat genes orthologous to the human interleukin-8 receptors. Dunstan CAN, Salafranca MN, Adhikari S, Xia Y, Feng L, Harrison JK. J. Biol. Chem. 271 (1996) 32770-32776.=20 Thanks again for your participation. See you next time, Gennady From=20poda@violet.incm.u-nancy.frWed Feb 12 09:05:32 1997 Date: 10 Feb 1997 08:40:38 -0800 From: Gennady PODA To: 7tms_r@net.bio.net Subject: 7tms: Chemokine Receptors in AIDS problem: a possible collaboratio= n?.. If you do not work on GPCRs mutagenesis and are not interested in AIDS problem please just discard this message.=20 Dear Netters, We are looking for a collaboration with an experimental group(s) doing site-directed mutagenesis of GPCRs.=20 We work on GPCRs modeling and our current interest is two chemokine receptors (CXCR-4/fusin and CC-CKR-5) which have been very recently found to be two main co-receptors for HIV envelop/host cells fusion. Currently, we have built a 3D model of CXCR-4 receptor and of its ligand, SDF-1, as well as for the ligand/receptor interaction. A 3D model of CKR-5 and docking proposals for its ligands (MIP-1alpha, MIP-1beta and RANTES) are hoped to come soon. In order to proceed we have used numerous mutagenesis data for Interleukin-8 alpha receptor as well as for IL8 itself. To day, the mutation data neither for those two receptors nor for their ligands (except some for RANTES) are not available, at least for us. So in this case the models can not be evaluated directly. But, being evaluated, they can be used to propose which residues should be substituted first according to the model.=20 So I would be extremely grateful if someone(s) of you let me know at your earliest convenience if some kind of collaboration can be initiated between us if you do have some interest in modeling. The so called feed-back could be established between our computational and your experimental group(s). If not, I would be very thankful if you kindly provide me with any references or information on it. I will summarize your quotations.=20 Thank you very much beforehand for your participation. I am looking forward to our possible fruitful for both side collaboration.=20 Sincerely yours, Gennady Poda ************************************************************ Dr. Gennady PODA Laboratoire de Chimie theorique (UA 510 du C.N.R.S.) Universite Henri Poincare, Nancy-I Faculte des Sciences - Domaine scientifique Victor Grignard B.P. 239 - 54506 Vandoeuvre-les-Nancy Cedex France Fax : + 33 (0)3.83.91.25.30 E-mail : poda@incm.u-nancy.fr ************************************************************ From owner-7tms_r@net.bio.net Sun Feb 16 22:00:00 1997 Path: biosci!VIOLET.INCM.U-NANCY.FR!poda From: poda@VIOLET.INCM.U-NANCY.FR (Gennady PODA) Newsgroups: bionet.molbio.proteins.7tms_r Subject: 7tms: GPCRs are dimers?!. Date: 17 Feb 1997 11:48:35 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 35 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hi, everybody! It is a long story if GPCRs can form dimers or not. M.Bouvier's group has published a paper with a direct biochemical evidence that beta2-AR can form dimers and suggested a possible role of this event in the receptor activation: T.Hebbert, S.Moffett, J.P.Morello, T.P.Loisel, D.G.Bichet, C.Barret and M.Bouvier. A peptide derived from a B2-adrenergic receptor transmembrane domain inhibits both receptor dimerization and activation. J. Biol. Chem. 271: 16384-16392, 1996. Does anyone know any other references about GPCRs dimerization? I will summarize the answers. Thanks a lot for your participation. With best wishes, Gennady ************************************************************ Dr. Gennady PODA Laboratoire de Chimie theorique (UA 510 du C.N.R.S.) Universite Henri Poincare, Nancy-I Faculte des Sciences - Domaine scientifique Victor Grignard B.P. 239 - 54500 Vandoeuvre-les-Nancy Cedex France Fax : (33) 03.83.91.25.30 E-mail : poda@incm.u-nancy.fr ************************************************************ From owner-7tms_r@net.bio.net Mon Feb 17 22:00:00 1997 Newsgroups: bionet.molbio.proteins.7tms_r Path: biosci!rutgers.rutgers.edu!uwm.edu!newsfeeds.sol.net!news.maxwell.syr.edu!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!howland.erols.net!rill.news.pipex.net!pipex!warwick!bris.ac.uk!usenet From: Andrew Doherty Subject: Re: 7tms: GPCRs are dimers?!. X-Nntp-Posting-Host: anaajd.ana.bris.ac.uk Content-Type: text/plain; charset=us-ascii To: Gennady PODA Message-ID: <33096B62.27BE@Bris.ac.uk> Sender: usenet@fsa.bris.ac.uk (Usenet) Content-Transfer-Encoding: 7bit Organization: University of Bristol, UK References: Mime-Version: 1.0 Date: Tue, 18 Feb 1997 08:42:10 GMT X-Mailer: Mozilla 3.0 (Win95; I) Lines: 50 Gennady PODA wrote: > > Hi, everybody! > > It is a long story if GPCRs can form dimers or not. M.Bouvier's group has > published a paper with a direct biochemical evidence that beta2-AR can > form dimers and suggested a possible role of this event in the receptor > activation: > > T.Hebbert, S.Moffett, J.P.Morello, T.P.Loisel, D.G.Bichet, C.Barret and > M.Bouvier. A peptide derived from a B2-adrenergic receptor transmembrane > domain inhibits both receptor dimerization and activation. J. Biol. Chem. > 271: 16384-16392, 1996. > > Does anyone know any other references about GPCRs dimerization? I will > summarize the answers. > > Thanks a lot for your participation. > > With best wishes, > > Gennady > Hi Gennady A recent paper on the metabotropic glutamate receptor mGlu5 suggests that it is a dimer. The reference is: Romano C., Yang W-L. & O'Malley K.L. (1996) Metabotropic glutamate receptor 5 is a disulfide linked dimer J.B.C. 271; 28612-28616 The mGlu's are not be in the same overall family as most GPCR's, sharing no real sequence homology with,say, the adremergic receptors, but they *are* 7-TM receptors, linked to G-proteins. So dimerisation may well be a common phenomenon among all GPCRs. Hope this is useful Andy D -- ************************************************************* Dr Andrew Doherty email - a.doherty@bris.ac.uk Dept. Anatomy Tel (0117)9287421 School of Medical Sciences Fax (0117)9287402 University of Bristol University Walk Bristol UK BS8 1TD ************************************************************* From owner-7tms_r@net.bio.net Tue Feb 18 22:00:00 1997 Path: biosci!VIOLET.INCM.U-NANCY.FR!poda From: poda@VIOLET.INCM.U-NANCY.FR (Gennady PODA) Newsgroups: bionet.molbio.proteins.7tms_r Subject: 7tms: "GPCRs are dimers?!." summary Date: 19 Feb 1997 01:44:39 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 198 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net - GPCRs are dimers? It seems that dimerization could be a quite common rule for GPCRs. Currently, there are experimental evidences about that for beta2-adrenergic, dopamine D2, type 1 angiotensin II, muscarinic M3, metabotropic glutamate (mGluRs), gonadotropin releasing hormone (GnRH) and yeast alpha-factor receptors. - How do they do that? Hebert T.E. et al. (1996) suggested that transmembrane domain VI of beta2-AR may represent part of an interface for receptor dimerization. Maggio R. et al. (1996) suggest that intermolecular interaction between muscarinic receptors depends on the presence of a long I3 loop. Romano et al. (1996) demonstrated a disulfide-dependent dimerization of the receptor which occurs within a large N-terminal extracellular domain. - Why GPCRs are dimers? It seems to be the most interesting question as well as the most difficult. Several groups showed that agonist but not antagonist binding stabilizes the dimeric state fo the receptor. So, probably, receptor dimerization may play an important role in coupling to G proteins. Any other isedas? Thanks for your participation. Gennady T.Hebbert, S.Moffett, J.P.Morello, T.P.Loisel, D.G.Bichet, C.Barret and M.Bouvier. A peptide derived from a B2-adrenergic receptor transmembrane domain inhibits both receptor dimerization and activation. J. Biol. Chem. 271: 16384-16392, 1996. Janovich J.A., Conn P.M. Gonadotropin releasing hormone agonist provokes homologous receptor microaggregation: an early event in seven-transmembrane receptor mediated signaling. Endocrinology 137: 3602-3605 (1996). Ng G.Y., O'Dowd B.F., Lee S.P., Chung H.T., Brann M.R., Seeman P., George S.R. Dopamine D2 receptor dimers and receptor-blocking peptides. Biochem. Biophys. Res. Commun. 227: 200-204 (1996). Ng G.Y., O'Dowd B.F., Caron M., Dennis M., Brann M.R., George S.R. Phosphorylation and palmitoylation of the human D2L dopamine receptor in Sf9 cells. J. Neurochem. 63: 1589-1595 (1994). Maggio R., Barbier P., Fornai F., Corsini G.U. Functional role of the third cytoplasmic loop in muscarinic receptor dimerization. J. Biol. Chem. 271: 31055-31060 (1996). Monnot C., Bihoreau C., Conchon S., Curnow K.M., Corvol P., Clauser E. Polar residues in the transmembrane domains of the type I angiotensin II receptor are required for binding and coupling. Reconstitution of the binding site by co-expression of two deficient mutants. J. Biol. Chem. 271(3): 1507-1513 (1996) Here are your answers: -------------------------------------------------------------------- Date:Mon, 17 Feb 1997 13:12:42 -0800 From: Ned David To: Gennady PODA Subject: Re: 7tms: GPCRs are dimers?!. We have some stuff to report. We work on the alpha-Factor receptor from Baker's Yeast. The receptor forms dimers and trimers by SDS-PAGE. They are not disulfides, as removing the cysteines has no effect on the phenomenon. The receptor migrates as monomers, dimers, and trimers over a sizing column. Additionally, we have some phenotypic evidence that the receptor forms oligomers in vivo. But that is a longer story. Feel free to call or write if you want the details on any of this. Regards, Nathaniel David, UCB Chemistry Dept ---------------------------------------------------------------------- Date: Tue, 18 Feb 1997 07:41:05 +0900 (JST) From: Yoshihisa Inoue To: poda@violet.incm.u-nancy.fr Subject: 7tms: GPCRs are dimers?!. Dear Dr. Gennady PODA >Does anyone know any other references about GPCRs dimerization? I will >summarize the answers. How about following reports. Dopamine D2 receptor dimers and receptor-blocking peptides: Ng et. al. BBRC 227:200-204 (1996) Polar residues in the transmembrane domains of the type I angiotensin II receptor are required for binding and coupling. Reconstitution of the binding site by co-expression of two deficient mutants. C.Monnot et al. J.Biol.Chem., 271(3):1507-1513 (1996) ____/ ___/ ___/ Yoshihisa INOUE (^_^) the Green Cross Corp. / / / 2-25-1 Shodai-Ohtani,Hirakata,Osaka 573 JAPAN / _ / / / tel: +81-720-56-9328 / / / / fax: +81-720-68-9597 _____/ _____/_____/ E-mail: inoue@greencross.co.jp ------------------------------------------------------------------------ From stanisla@ohsu.EDUTue Feb 18 09:34:30 1997 Date: Mon, 17 Feb 1997 14:57:07 -0800 (PST) From: "Dinesh Stanislaus, Oregon Regional Primate Research Center" To: Gennady PODA Subject: Re: 7tms: GPCRs are dimers?!. Hi Gennady, P. Michael Conn's group have shown evidence for GPCR dimerization. They concentrated on the GnRH receptor. I don't have the references off the top of my head, but the papers were in Nature and in Endocrinology. Hope this is helpful to you. Good Luck D. Stanislaus ------------------------------------------------------------------------ From A.Doherty@Bris.ac.ukTue Feb 18 16:43:56 1997 Date: Tue, 18 Feb 1997 08:42:10 GMT From: Andrew Doherty To: 7tms_r@net.bio.net, Gennady PODA Subject: Re: 7tms: GPCRs are dimers?!. Hi Gennady A recent paper on the metabotropic glutamate receptor mGlu5 suggests that it is a dimer. The reference is: Romano C., Yang W-L. & O'Malley K.L. (1996) Metabotropic glutamate receptor 5 is a disulfide linked dimer J.B.C. 271; 28612-28616 The mGlu's are not be in the same overall family as most GPCR's, sharing no real sequence homology with,say, the adremergic receptors, but they *are* 7-TM receptors, linked to G-proteins. So dimerisation may well be a common phenomenon among all GPCRs. Hope this is useful Andy D -- ************************************************************* Dr Andrew Doherty email - a.doherty@bris.ac.uk Dept. Anatomy Tel (0117)9287421 School of Medical Sciences Fax (0117)9287402 University of Bristol University Walk Bristol UK BS8 1TD ************************************************************* ************************************************************ Dr. Gennady PODA Laboratoire de Chimie theorique (UA 510 du C.N.R.S.) Universite Henri Poincare, Nancy-I Faculte des Sciences - Domaine scientifique Victor Grignard B.P. 239 - 54500 Vandoeuvre-les-Nancy Cedex France Fax : (33) 03.83.91.25.30 E-mail : poda@incm.u-nancy.fr ************************************************************ From owner-7tms_r@net.bio.net Wed Feb 19 22:00:00 1997 Path: biosci!ITSA.UCSF.EDU!pchi From: pchi@ITSA.UCSF.EDU (Patty P Chi) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 20 Feb 1997 15:18:11 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 4 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Does anyone know if CHO or HEK293 cells have any endogenous Gq or Gi coupled receptors? From owner-7tms_r@net.bio.net Wed Feb 19 22:00:00 1997 Path: biosci!YALE.EDU!henrik.dohlman From: henrik.dohlman@YALE.EDU ("Henrik Dohlman") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: 7tms- "GPCRs are dimers Date: 20 Feb 1997 13:48:46 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 69 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Reply to: RE>>7tms: "GPCRs are dimers?!." summary I recently asked R. Henderson to shed some light on this very question. While his projection maps indicate that rhodopsin assembles in a dimer, I think it is safe to say he feels that the conditions for crystallization are sufficiently different from those normally found in cells that you can't draw a conclusion one way or another from these data. Henrik Dohlman henrik.dohlman@yale.edu -------------------------------------- Date: 2/20/97 4:09 PM To: Henrik Dohlman From: TJ Murphy Gennady PODA wrote: > > - GPCRs are dimers? > > It seems that dimerization could be a quite common rule for GPCRs. > Currently, there are experimental evidences about that for > beta2-adrenergic, dopamine D2, type 1 angiotensin II, muscarinic M3, > metabotropic glutamate (mGluRs), gonadotropin releasing hormone (GnRH) and > yeast alpha-factor receptors. etc I think it is important to consider this interesting discussion in light (no pun intended) of the electron density mapping of rhodopsin, which would be expected to provide the most direct possible evidence for this concept. In looking at its map, one is hard pressed to interpret the contour depiction of the data as supportive of GPCR multimerization. In contrast, a similar but cruder technique has shown clearly that nicotinic receptors are composed of a multimeric assembly of polypeptide subunits. Schertler, G.F.X., Villa, C. and R. Henderson. Projection structure of rhodopsin. Nature 363:770-772 (1993). -- T.J. Murphy 404-727-2467 Assistant Professor 404-727-0365 (fax) Emory University School of Medicine medtjm@bimcore.emory.edu Department of Pharmacology http://www.emory.edu/PHARMACOLOGY/MURPHY/ 1510 Clifton Road Atlanta, GA 30322 ------------------ RFC822 Header Follows ------------------ Received: by QuickMail.Yale.edu with SMTP;20 Feb 1997 16:06:21 -0500 Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id LAA25866; Thu, 20 Feb 1997 11:49:25 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id LAA25860; Thu, 20 Feb 1997 11:49:25 -0800 To: 7tms_r@net.bio.net From: TJ Murphy Subject: Re: 7tms: "GPCRs are dimers?!." summary Date: Thu, 20 Feb 1997 14:42:03 -0500 Message-ID: <330CA90B.9FC@bimcore.emory.edu> NNTP-Posting-Host: murphy1.pharm.emory.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (Win95; I) From owner-7tms_r@net.bio.net Wed Feb 19 22:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!cam-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!mindspring!cssun.mathcs.emory.edu!news.service.emory.edu!news From: TJ Murphy Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: 7tms: "GPCRs are dimers?!." summary Date: Thu, 20 Feb 1997 14:42:03 -0500 Organization: Emory University Lines: 32 Message-ID: <330CA90B.9FC@bimcore.emory.edu> References: NNTP-Posting-Host: murphy1.pharm.emory.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (Win95; I) Gennady PODA wrote: > > - GPCRs are dimers? > > It seems that dimerization could be a quite common rule for GPCRs. > Currently, there are experimental evidences about that for > beta2-adrenergic, dopamine D2, type 1 angiotensin II, muscarinic M3, > metabotropic glutamate (mGluRs), gonadotropin releasing hormone (GnRH) and > yeast alpha-factor receptors. etc I think it is important to consider this interesting discussion in light (no pun intended) of the electron density mapping of rhodopsin, which would be expected to provide the most direct possible evidence for this concept. In looking at its map, one is hard pressed to interpret the contour depiction of the data as supportive of GPCR multimerization. In contrast, a similar but cruder technique has shown clearly that nicotinic receptors are composed of a multimeric assembly of polypeptide subunits. Schertler, G.F.X., Villa, C. and R. Henderson. Projection structure of rhodopsin. Nature 363:770-772 (1993). -- T.J. Murphy 404-727-2467 Assistant Professor 404-727-0365 (fax) Emory University School of Medicine medtjm@bimcore.emory.edu Department of Pharmacology http://www.emory.edu/PHARMACOLOGY/MURPHY/ 1510 Clifton Road Atlanta, GA 30322 From owner-7tms_r@net.bio.net Thu Feb 20 22:00:00 1997 Path: biosci!daresbury!nntp-trd.UNINETT.no!news.uit.no!news From: edvard@fagmed.uit.no () Newsgroups: bionet.molbio.proteins.7tms_r Subject: Usage of GRAP mutant databases Date: 21 Feb 1997 13:50:04 GMT Organization: Univ. of Tromso, IMB Lines: 30 Message-ID: <5ek96c$346@news.uit.no> Reply-To: edvard@fagmed.uit.no NNTP-Posting-Host: fa13.imb.fm.uit.no As you all are aware of (I hope) we're running the GRAP/tinyGRAP mutant databases at http://www-grap.fagmed.uit.no/GRAP/homepage.html. We can see from our Web-server log-files that you retrieve data from our databases, but we would like to ask you the following questions: 1) In what way is the databases useful to YOU? 2) For what purpose(s) do YOU use the databases? Please do respond, but DO NOT POST THE RESPONSE TO THIS NEWSGROUP!!! Instead fill up MY MAILBOX edvard@fagmed.uit.no, and I'll put together a summary for the newsgroup. Thanks in advance for your cooperation! OEyvind. -o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o- ___ _ | /| _| |_ _| __ _ _| _ _ |/_| \/ \/ | |\| |_| |_ |_| \/ |_|_ | |_| _\ |_' |\| / _____________________________________________________________________________ School of Medicine | Dept. of Pharmacology, IMB | TelePhone: +47 77 64 53 42 University of Tromsoe | TeleFax: +47 77 64 53 10 MH, Breivika | Email: edvard@fagmed.uit.no N-9037 TROMSOE, NORWAY | URL: http://atf1.fagmed.uit.no/mgl.html ------------------------------------------------------------------------------ From owner-7tms_r@net.bio.net Sat Feb 22 22:00:00 1997 Path: biosci!ihnp4.ucsd.edu!news1.ucsd.edu!usenet From: Kevin Shreder Newsgroups: bionet.molbio.proteins.7tms_r Subject: The Antibody Resource Page Date: Sun, 23 Feb 1997 18:29:38 -0700 Organization: Antibody Resource Page Lines: 33 Message-ID: <3310EF02.7861@znet.com> NNTP-Posting-Host: chegood10.ucsd.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) The Antibody Resource Page (ARP) has recently been updated and given a new user-friendly look. The ARP is divided up into 7 sections: 1. Educational Resources - links to pages on antibodies that will interest the novice and expert alike 2. Online Databanks and Databases - links to scientific databases in the area of sequence analysis and hybridoma work 3. Online Journals 4. How to Find an Antibody - a section for ways to find commercial sources (online or otherwise) of antibodies. If you are a researcher who works with antibodies, you cannot afford to miss this section. 5. Online Companies - a large list of online companies (over 75) that sell antibodies or antibody related products. There is also a section for companies that are not online. 6. Miscellaneous - links to various immunological and biotechnology webpages 7. Antibody Gallery - a new addition to the ARP. This is a section where researchers can donate pictures of antibodies for educational purposes. If you have something to donate, please contact me. The ARP is designed for both beginners and experts who are looking for information about antibodies. I am always looking for new links, so if you know of something, please contact me. Or just contact me to let me know what you think of the page! The URL for the Antibody Resource Page is: http://www-chem.ucsd.edu/Faculty/goodman/antibody.html/abpage.html Kevin Shreder, Ph.D. kshreder@znet.com From owner-7tms_r@net.bio.net Mon Feb 24 22:00:00 1997 Path: biosci!MAIL.MED.CORNELL.EDU!mjcann From: mjcann@MAIL.MED.CORNELL.EDU (Martin Cann) Newsgroups: bionet.molbio.proteins.7tms_r Subject: 7tmr and yeast 2-hybrid Date: 25 Feb 1997 10:06:25 -0800 Organization: Cornell University Medical College Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: <33132A84.11A7@mail.med.cornell.edu> NNTP-Posting-Host: net.bio.net I am interested in looking for a potential ligand for a 7tmr using the yeast 2 hybrid. Obviously I cannot use the entire protein as a bite but it has an extracellular N-terminal extension that I am hoping to use. My question is, what is the likelihood that this is the ligand binding site? Is it more likely to be this region in conjunction with the tm domains? What are my chances? Has anyone tried this? Please reply to me directly. Martin Cann mjcann@med.cornell.edu From owner-7tms_r@net.bio.net Mon Feb 24 22:00:00 1997 Path: biosci!MAIL.MED.CORNELL.EDU!mjcann From: mjcann@MAIL.MED.CORNELL.EDU (Martin Cann) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Adenylyl cyclase subtypes Date: 25 Feb 1997 10:10:02 -0800 Organization: Cornell University Medical College Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <33132B5E.4E17@mail.med.cornell.edu> NNTP-Posting-Host: net.bio.net The unfortunate truth is that just about every cyclase reference involves expression in primarily 293's or baculo system. Methods in Enzymology vol. 238 has chapters on how to express in 293, CHO (not much used really), or baculo systems. If you need something more specific mail me. Martin Cann mjcann@med.cornell.edu From owner-7tms_r@net.bio.net Tue Feb 25 22:00:00 1997 Path: biosci!nch.go.jp!gtsujimoto From: gtsujimoto@nch.go.jp (Gozoh Tsujimoto) Newsgroups: bionet.molbio.proteins.7tms_r Subject: knockout mice of alpha1-adrenoceptor subtype Date: 26 Feb 1997 19:38:13 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <199702270339.MAA15594@nch.go.jp> NNTP-Posting-Host: net.bio.net We are now interested in making knockout mice of alpha1-adrenoceptor subtype. Has anyone tried or is in progress ? Also, collaboration would be welcomed. Please reply to me directly. ******************************************* Gozoh Tsujimoto, M.D. Ph.D. Department of Molecular, Cell Pharmacology National Children's Medical Research Center 3-35-31, Taishi-do, Setagaya-Ku, Tokyo 154, JAPAN TEL 81-3-3414-2476 FAX 81-3-3419-1252 e-mail:gtsujimoto@nch.go.jp ******************************************* From owner-7tms_r@net.bio.net Wed Feb 26 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!news.sgi.com!howland.erols.net!surfnet.nl!highway.leidenuniv.nl!RULY81 From: ferro@rullf2.leidenuniv.nl (Wouter Ferro) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Symposium on Homologous Recombination, Wageningen, The Netherlands, April 11, 1997 Date: Thu, 27 Feb 1997 14:15:16 GMT Organization: Leiden University, The Netherlands Lines: 62 Message-ID: <5f450e$3a3@highway.leidenuniv.nl> NNTP-Posting-Host: ruly81.medfac.leidenuniv.nl X-Newsreader: News Xpress 2.01 Symposium on Homologous Recombination, Wageningen, The Netherlands, April 11, 1997 The Dutch Genetical Society (Nederlandse Genetische Vereniging) Presents Symposium on HOMOLOGOUS RECOMBINATION April 11, 1997 Entrance is free for all interested persons! Location: WICC/IAC Lawickse Allee 11, Wageningen, The Netherlands Homologous recombination is an essential process for nearly all organisms. It plays an important role in the creation of genetic diversity and in the repair of DNA damage. The research into the process has advanced rapidly in the past few years. Therefor the Society is happy that several of the most important scientists in the field are willing to give presentations that will form an up to date picture of our knowledge on HOMOLOGOUS RECOMBINATION. In just one day you can catch up; an occasion that will not soon be repeated in the Netherlands! Programme: 9.30 - 10.15 Coffee 10.15 - 11.00 S. West, ICRF (Herts, UK): The proteins of genetic recombination: Functional conservation from bacteria to man 11.00 - 11.45 A. Nicolas, Inst. Curie (Paris, France): Meiotic recombination in the yeast Saccharomyces cerevisiae 11.45 - 12.30 N. Kleckner, Harvard University (Cambridge, MA, USA): Meiotic recombination: biology and biochemistry 12.30 - 13.30 Lunch 13.30 - 14.15 A. Pastink, Leiden University (Leiden, The Netherlands): Recombinational repair of double-strand breaks in lower and higher eukaryotes 14.15 - 15.00 R. Kanaar, Erasmus Univ. (Rotterdam, the Netherlands): Homology-dependent recombination repair in mammals 15.00 - 15.15 Tea 15.15 - 16.00 H. te Riele, Netherlands Cancer Inst. (Amsterdam, The Netherlands): Phenotypic consequences of mismatch repair deficiency in cells and mice 16.00 - 16.45 D. van Gent, Erasmus Univ. (Rotterdam, the Netherlands): The mechanism of V(D)J recombination Organization: J.H.J. Hoeijmakers and C. Heyting Lunch (fl 16,50) can be ordered by fax (0317-483146) or mail (Lab. voor Erfelijkheidsleer, Dreijenlaan 2, 6703 HA Wageningen). The WICC/IAC in Wageningen can be reached from NS-station Ede-Wageningen by ‘treintaxi’ or by bus 83 of 84 (towards Wageningen busstation). From NS-station Arnhem by bus 50, 80 of 81 (towards Wageningen busstation). From the busstation to the WICC/IAC is a few minutes walk. Further information: W. Ferro, secretary NGV, e-mail: ferro@RULLF2.LeidenUniv.nl C. Heyting, e-mail: Christa.Heyting@molcelgen.el.wau.nl From owner-7tms_r@net.bio.net Wed Feb 26 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!cs.utexas.edu!swrinde!howland.erols.net!ix.netcom.com!news From: Rich Cox Newsgroups: bionet.molbio.proteins.7tms_r Subject: Subscribe Date: Thu, 27 Feb 1997 23:09:57 -0500 Organization: Netcom Lines: 1 Message-ID: <33165A95.62AD@ix.netcom.com> NNTP-Posting-Host: rtp-nc4-15.ix.netcom.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-NETCOM-Date: Thu Feb 27 8:06:59 PM PST 1997 X-Mailer: Mozilla 3.01-C-AICK1-2 (Macintosh; I; 68K) Subscribe From owner-7tms_r@net.bio.net Thu Feb 27 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!news-peer.gsl.net!news.gsl.net!news.maxwell.syr.edu!cpk-news-hub1.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!howland.erols.net!news.sprintlink.net!news-peer.sprintlink.net!newsfeed.internetmci.com!news.dtinet.or.jp!ppp2!aisoai From: aisoai@agc.co.jp (ISOAI Atsushi) Newsgroups: bionet.molbio.proteins.7tms_r Subject: WWW for the Molecular Biologists and Biochemists Date: Fri, 28 Feb 1997 23:22:09 +0900 Organization: Research Center, Asahi Glass Co.LTD. Lines: 15 Message-ID: NNTP-Posting-Host: ppp2.yokohama2.dtinet.or.jp Mime-Version: 1.0 Content-Type: text/plain; charset=iso-2022-jp X-Newsreader: NewsWatcher-J 2.0J16 Hi all netters; I have uploaded Web site for the biochemists and molecular biologists. In my site, you can find very useful database, www, ftp, gopher and other useful sites on the net. Moreover, my sites present many sites which offer you software or tools for the research. Please visit and enjoy! [Sites for the Molecular Biology - LINKS] URL: http://www.yk.rim.or.jp/~aisoai/index.html I look forward to hearing your suggestions and comments from you. ================================================================= Atsushi Isoai, Ph.D. Senior Staff Researcher, Research Center, Asahi Glass Co.LTD. URL: http://www.yk.rim.or.jp/~aisoai/index.html ================================================================= From owner-7tms_r@net.bio.net Fri Feb 28 22:00:00 1997 Path: biosci!MAIL.MED.CORNELL.EDU!mjcann From: mjcann@MAIL.MED.CORNELL.EDU (Martin Cann) Newsgroups: bionet.molbio.proteins.7tms_r Subject: 7tmr and yeast 2 hybrid: reprise Date: 1 Mar 1997 06:04:44 -0800 Organization: Cornell University Medical College Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <331837E4.2C4B@mail.med.cornell.edu> NNTP-Posting-Host: net.bio.net Many thanks to everybody for there help. For those people out there who were also interested, I think the bottom line is that the chances of this working depend upon the class of receptor, the likely nature of your ligand and where related ligands are known to bind, the folding of the N terminus of the receptor in the 2 hybrid system, and a little bit of luck. Martin