From owner-7tms_r@net.bio.net Thu May 01 23:00:00 1997 Path: biosci!daresbury!uninett.no!stdio!news-feed.inet.tele.dk!news.maxwell.syr.edu!news-peer.sprintlink.net!news.sprintlink.net!Sprint!newsjunkie.ans.net!newsfeeds.ans.net!lantana.singnet.com.sg!newsfeed.singnet.com.sg!id4.nus.edu.sg!nuscc.nus.sg!sbsdjl From: sbsdjl@leonis.nus.sg (ding jeak ling) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Protein modelling job vacancies Date: 3 May 1997 02:03:42 GMT Organization: National University of Singapore Lines: 29 Message-ID: <5ke6du$pf8@nuscc.nus.sg> NNTP-Posting-Host: sbsdjl@leonis.nus.sg X-Newsreader: TIN [version 1.2 PL2] ......................................................................... "Positions available - for a period of 2 years" (1) Research Fellow with PhD. Prerequisites include experience in computer aided molecular modelling of macroproteins, ligand-binding and conformational studies. Creative flair and ability to solve problems, as exemplified by a strong publication track record are helpful. Salary: commensurate with experience, up to a maximum of US$47,000 per annum (inclusive of 13th month payment and variable bonuses). (2) Research Assistant with BSc Hons. in Cell and Molecular Biology. To carry out genetic engineering experiments and research and development in gene expression. Knowledge in computational molecular biology will be an advantage. Salary: commensurate with qualification and experience, up to US25,000 per annum. (Current Exchange rate is US$1 = S$1.41) Interested applicants should write/enquire and send CV to: JL Ding (PhD) Marine Biotechnology Laboratory School of Biological Sciences National University of Singapore Kent Ridge, Singapore 119260 Tel: 65-7722776 Fax: 65-7792486 E.mail:sbsdjl@leonis.nus.sg .............................................................................. From owner-7tms_r@net.bio.net Sun May 04 23:00:00 1997 Path: biosci!nunet.neu.edu!r.deth From: r.deth@nunet.neu.edu ("Richard Deth") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Solubilization and HPLC Date: 5 May 1997 12:07:17 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net 1. Does anyone know of the successful application of HPLC for the separation and isolation of a G protein-coupled receptor? e.g. a paper in the literature or a personal knowledge? 2. Awhile ago there was a discussion about the selection of detergents for solubilization of GPCRs, however, I don't recall the consensus recommendation if there was one. Is there something better than NP-40?? Thanks in advance for the help! r.deth@nunet.neu.edu From owner-7tms_r@net.bio.net Tue May 06 23:00:00 1997 Path: biosci!BIO.VU.NL!hind From: hind@BIO.VU.NL (Hind van Tol-Steye) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Arachidonic acid Date: 7 May 1997 01:06:20 -0700 Organization: Vrije Universiteit Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <3370382D.3302@bio.vu.nl> Reply-To: hind@bio.vu.nl NNTP-Posting-Host: net.bio.net Is there anybody who has experience with handling arachidonic acid (AA)for functional studies? I want to add AA to cells and measure the electrophysiological response, but I have difficulties to prepare proper solutions of AA. Some solutions give raise to a response, whereas other solutions (prepared in the same way, under nitrogen), tested on the same cell do not. Perhaps there are problems with solubility, oxidation and/or sticking to glass ware? Any tips to make solutions of AA in a reproducible way? Is there any difference in quality between AA from different companies? -- Hind van Tol-Steye Vrije Universiteit De Boelelaan 1083 1081 HV Amsterdam From owner-7tms_r@net.bio.net Sat May 10 23:00:00 1997 Path: biosci!CHEMPATH.UCT.AC.ZA!DAVID From: DAVID@CHEMPATH.UCT.AC.ZA ("Dave") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Moderation Date: 11 May 1997 22:24:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I agree that this group should be moderated. I think that spam mail is just going to get more and more common, as there are now web sites that offer "Advertising that reaches tens of thousands of people"! I recently discovered a way to stop spammer from getting your email address. They normally get it from web sites where people must fill in some form or other and leave their email. One trick is to add a tilde (~) character to the start of your email address. The spammers use an automatic system to get emails, so they probably won't notice the tilde. When they try and email that address, it won't work. If you own a web page, do the same kind of thing, but leave a message saying "remove tilde for actual address". Unfortunately, somebody somewhere may already have your email address, but this trick might help a bit. -------------------------------------------------------------------- Dave Myburgh Lab 227 Falmouth Bldg E-mail: david@chempath.uct.ac.za Dept. Chemical Pathology mybdav02@uctvms.uct.ac.za UCT Medical School Phone: 406-6359 Observatory FAX: 448-8150 7925 South Africa -------------------------------------------------------------------- From owner-7tms_r@net.bio.net Sat May 10 23:00:00 1997 Path: biosci!daresbury!uninett.no!nntp.uio.no!news.maxwell.syr.edu!cs.utexas.edu!geraldo.cc.utexas.edu!netnews.uthscsa.edu!kolakowski.uthscsa.edu!kolakowski From: Frank Kolakowski Newsgroups: bionet.molbio.proteins.7tms_r Subject: Moderation Vote Date: 11 May 1997 23:33:11 GMT Organization: Dept. of Pharmacology - UTHSCSA Lines: 32 Distribution: world Message-ID: <5l5kvn$7t2$1@cosmos.uthscsa.edu> NNTP-Posting-Host: kolakowski.uthscsa.edu Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Newsreader: Nuntius 2.0.4_PPC X-XXMessage-ID: X-XXDate: Sun, 11 May 1997 23:25:51 GMT I am willing to moderate the contents of this news group. We have a relatively small volume of messages overall. I believe that this makes the number of SPAM messages seem very large. The effect is very noticable however. After every spam posting, there is a marked increase of misdirected unsubscribe messages. Please comment to this group. I will tally the responses in one week. Frank Kolakowski Postal Address Dr. Lee F. (Frank) Kolakowski, Jr. Department of Pharmacology University of Texas Health Science Center at San Antonio 7703 Floyd Curl Drive San Antonio, Texas 78284-7764 Telephone 210-567-4233 210-567-4303 (Fax) 210-567-4178 (Lab) Email kolakowski@uthscsa.edu lfk@receptor.mgh.harvard.edu World Wide Web http://kolakowski.uthscsa.edu/ http://receptor.mgh.harvard.edu/GCRDBHOME.html From owner-7tms_r@net.bio.net Sat May 10 23:00:00 1997 Path: biosci!UH.EDU!RABond From: RABond@UH.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 11 May 1997 16:03:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IIR3IXITQ0000658@Post-Office.UH.EDU> NNTP-Posting-Host: net.bio.net unsubscribe Richard A. Bond, Ph.D.: Department of Pharmacological and Pharmaceutical Sciences, University of Houston, 4800 Calhoun, Houston, Texas 77204-5515. E-mail: RABond@UH.edu telephones: office: (713) 743-1210, lab: (713) 743-1219 fax: (713) 743-1229. From owner-7tms_r@net.bio.net Sun May 11 23:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.proteins.7tms_r Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 12 May 1997 02:00:39 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705120900.CAA08973@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-7tms_r@net.bio.net Sun May 11 23:00:00 1997 Path: biosci!BLUE.INCM.U-NANCY.FR!maigret From: maigret@BLUE.INCM.U-NANCY.FR Newsgroups: bionet.molbio.proteins.7tms_r Subject: moderation vote Date: 12 May 1997 09:35:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net OK for moderation ------------------------------------------------------------- MAIGRET Bernard Directeur de Recherche au CNRS Directeur de l'UA CNRS 510 "Interactions Moleculaires" Lab.Chimie Theorique Universite Henri Poincare B.P. 239 54506 Vandoeuvre-les-Nancy FRANCE tel: 33-3-83.91.25.28 fax: 33-3-83.91.25.30 e-mail: maigret@incm.u-nancy.fr ------------------------------------------------------------- From owner-7tms_r@net.bio.net Sun May 11 23:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.maxwell.syr.edu!news-peer.sprintlink.net!news.sprintlink.net!Sprint!ais.net!uunet!in3.uu.net!198.82.160.249!solaris.cc.vt.edu!newsgate.duke.edu!kwatra2.mc.duke.edu!user From: edroush@acpub.duke.edu (Eric Roush) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Moderation Vote Date: Mon, 12 May 1997 15:21:29 -0400 Organization: Glow-n-the-Dark Radiochemicals, Inc. Lines: 13 Distribution: world Message-ID: References: <5l5kvn$7t2$1@cosmos.uthscsa.edu> NNTP-Posting-Host: kwatra2.mc.duke.edu In article <5l5kvn$7t2$1@cosmos.uthscsa.edu>, Frank Kolakowski wrote: > I am willing to moderate the contents of this news group. More power to you; you've got my vote. -- Eric Roush When an eel edroush@acpub.duke.edu bites your heel, also coache@aol.com makes you squeal, pain you feel, that's a moray. From owner-7tms_r@net.bio.net Sun May 11 23:00:00 1997 Path: biosci!RUBENS.ITS.UNIMELB.EDU.AU!pms From: pms@RUBENS.ITS.UNIMELB.EDU.AU Newsgroups: bionet.molbio.proteins.7tms_r Subject: moderation Date: 12 May 1997 15:52:38 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IITCVXI5Q68WXOGD@rubens.its.unimelb.edu.au> NNTP-Posting-Host: net.bio.net I support moderation of the news group. ---------------------------------------------------------------------------- ------------- Patrick M. Sexton, PhD St. Vincent's Institute of Medical Research 41 Victoria Parade, Fitzroy 3065, Victoria, AUSTRALIA Phone: 61-3-9288-2480 Fax: 61-3-9416-2676 E-mail: pms@rubens.its.unimelb.edu.au From owner-7tms_r@net.bio.net Sun May 11 23:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news.apfel.de!uni-erlangen.de!winx03!wpxx02!not-for-mail From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: moderation Date: Mon, 12 May 1997 19:29:16 +0200 Organization: University of Wuerzburg, Germany Lines: 14 Distribution: world Message-ID: References: <9705121310.AA03830@alcor.bms.com> NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Stanley Krystek (krystek@ALCOR.BMS.COM) wrote: > I vote for a moderated group and Frank can have > my support. Me too. (BTW, is there an open news server which carries a decent 7tms feed? I seem to miss at least 50% of the postings -- I hope this also includes 50% of the "make money fast" stuff :-).) --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP3 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-7tms_r@net.bio.net Sun May 11 23:00:00 1997 Path: biosci!WELCHLINK.WELCH.JHU.EDU!bjmarg From: bjmarg@WELCHLINK.WELCH.JHU.EDU (Vinnie Terranova) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Moderation Date: 12 May 1997 10:22:54 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Please, yes, let's moderate. Thanks for the offer, Frank! -Barry Margulies, Ph.D. (bjmarg@welchlink.welch.jhu.edu) URL: http://www.welch.jhu.edu/~bjmarg/homepage.html *************************************************************************** The most exciting phrase to hear in science, the one that heralds new discoveries, is not "Eureka!" but "That's funny ..." - Isaac Asimov *************************************************************************** From owner-7tms_r@net.bio.net Sun May 11 23:00:00 1997 Path: biosci!SPINE.MED.UTORONTO.CA!milton From: milton@SPINE.MED.UTORONTO.CA (Milton Charlton) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Moderation Vote Date: 12 May 1997 07:47:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <5l5kvn$7t2$1@cosmos.uthscsa.edu> NNTP-Posting-Host: net.bio.net Please moderate. M. Charlton From owner-7tms_r@net.bio.net Sun May 11 23:00:00 1997 Path: biosci!BIOCELL.IRMKANT.RM.CNR.IT!7tm From: 7tm@BIOCELL.IRMKANT.RM.CNR.IT (Transmembrane receptors) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Moderation Vote Date: 12 May 1997 08:05:19 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net OK for moderation 7TM Group Istituto Biologia Cellulare Consiglio Nazionale delle Ricerche I-00137 Roma Italy ------- From owner-7tms_r@net.bio.net Sun May 11 23:00:00 1997 Path: biosci!ALCOR.BMS.COM!krystek From: krystek@ALCOR.BMS.COM (Stanley Krystek) Newsgroups: bionet.molbio.proteins.7tms_r Subject: moderation Date: 12 May 1997 06:09:34 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <9705121310.AA03830@alcor.bms.com> NNTP-Posting-Host: net.bio.net I vote for a moderated group and Frank can have my support. stan Stanley R. Krystek, Jr., Ph.D. Bristol-Myers Squibb Pharmaceutical Research Institute Department of Macromolecular Modeling P.O. Box 4000, Room H.3812, Princeton, N.J. 08543-4000 (609) 252-4100 FAX: (609) 252-6030 INTERNET: krystek@bms.com From owner-7tms_r@net.bio.net Sun May 11 23:00:00 1997 Path: biosci!bio.gla.ac.uk!j.a.t.dow From: j.a.t.dow@bio.gla.ac.uk (Julian Dow) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: moderation Date: 12 May 1997 06:54:13 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net >I vote for a moderated group and Frank can have >my support. > Me too. By the way, did I tell you about this great opportunity to earn $1000's for no effort..? -------------------------------------------------------- Dr Julian Dow - Molecular Genetics - Pontecorvo Building - University of Glasgow - Glasgow G11 6NU - UK Phone: +44 (0)141 330 4616 FAX: +44 (0)141 330 5994 Email: j.a.t.dow@bio.gla.ac.uk WWW: http://www.mblab.gla.ac.uk/tubules/ From owner-7tms_r@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!howland.erols.net!newsxfer.itd.umich.edu!qiclab.scn.rain.com!news.teleport.com!not-for-mail From: nevek@teleport.com (Kim Neve) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: moderation Date: Tue, 13 May 1997 14:39:01 GMT Organization: Oregon Health Sciences University Lines: 16 Message-ID: <33787c98.1013382126@news.teleport.com> References: <9705121310.AA03830@alcor.bms.com> NNTP-Posting-Host: ip-pdx06-07.teleport.com X-Newsreader: Forte Free Agent 1.1/32.230 krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) wrote: >Stanley Krystek (krystek@ALCOR.BMS.COM) wrote: >> I vote for a moderated group and Frank can have >> my support. > >Me too. (BTW, is there an open news server which carries a decent >7tms feed? I seem to miss at least 50% of the postings -- I hope >this also includes 50% of the "make money fast" stuff :-).) > I also vote yes, and also would like to know abou an open news server, since I also miss many of the postings. For example, I never saw Frank's original offer to moderate the group. Kim Neve From owner-7tms_r@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!VAX.GRC.NIA.NIH.GOV!chahrzad From: chahrzad@VAX.GRC.NIA.NIH.GOV Newsgroups: bionet.molbio.proteins.7tms_r Subject: moderate Date: 13 May 1997 09:32:00 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: <009B4322.39101991.328@vax.grc.nia.nih.gov> NNTP-Posting-Host: net.bio.net vote for moderate Thank you Chahrzad Montrose From owner-7tms_r@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!swrinde!cs.utexas.edu!geraldo.cc.utexas.edu!netnews.uthscsa.edu!kolakowski.uthscsa.edu!kolakowski From: Frank Kolakowski Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Moderation Vote (Moderation will be happening) Date: 13 May 1997 23:34:07 GMT Organization: Dept. of Pharmacology - UTHSCSA Lines: 30 Distribution: world Message-ID: <5latpf$mi1$1@cosmos.uthscsa.edu> References: <5l5kvn$7t2$1@cosmos.uthscsa.edu> NNTP-Posting-Host: kolakowski.uthscsa.edu Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Newsreader: Nuntius 2.0.4_PPC X-XXMessage-ID: X-XXDate: Tue, 13 May 1997 23:27:52 GMT 7TMS_R Readers/Posters: I have been swamped by email (>50 messages) regarding the moderation issue. There has not been a single posting or email which does not want the news group to be moderated. I have begun to take the steps necessary to make 7tms_r a moderated newsgroup. Dave Kristofferson has emailed me about the changes to the newsgroup's charter necessary for moderation. Specifically we must have a policy about what kinds of messages are acceptable. The following text is the policy from the plant signal transduction newsgroup: Moderation Policy: Mass-posted commercial messages, chain letters, and similar postings not germane to plants or signal transduction will be deleted without comment. Inappropriate messages posted in good faith will be returned to the sender. Messages not strictly within the charter but likely to be of interest to many subscribers (e.g., messages dealing with certain aspects of signal transduction in animal systems) will be accepted. Use of the newsgroup for commercial purposes is prohibited. This looks good to me with a little bit of editing. As soon as this is finished, it will take about 2-4 days to change the status of the group to a moderated group. No more SPAM, subscribe, or unsubscribe messages. Frank Kolakowski From owner-7tms_r@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!news-peer.sprintlink.net!news-pull.sprintlink.net!news.sprintlink.net!Sprint!news-feed1.tiac.net!uunet!in1.uu.net!136.142.185.26!newsfeed.pitt.edu!dsinc!netnews.upenn.edu!brass6.med.upenn.edu!user From: obrien@pharm.med.upenn.edu (:-Peter) Newsgroups: bionet.molbio.proteins.7tms_r Subject: moderation schmoderation Date: 13 May 1997 19:04:46 GMT Organization: U. of Pennsylvania Lines: 8 Message-ID: NNTP-Posting-Host: brass6.med.upenn.edu I vote for extremism. Barring that, let's keep out the spam. :-Peter From owner-7tms_r@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!newsfeed.nacamar.de!news.apfel.de!univ-lyon1.fr!news.ujf-grenoble.fr!ciril.fr!not-for-mail From: Fabien Campagne Newsgroups: bionet.molbio.proteins.7tms_r Subject: Change of Viseur prog. distribution policy Date: Tue, 13 May 1997 20:59:26 +0100 Organization: Laboratoire de Chimie The'orique de Nancy Lines: 18 Message-ID: <3378C81E.41C6@incm.u-nancy.fr> NNTP-Posting-Host: yellow.incm.u-nancy.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0SC-SGI (X11; I; IRIX64 6.2 IP28) I mailed several month ago a message to this newsgroup to describe the Viseur program. At that time, this program was available only to non profit laboratories. The Viseur program is now available to the indutrial world (at no or a few exceptions). The new distribution policy for the program is described at: http://www.lctn.u-nancy.fr/viseur/DistributionPolicy.html For more information on the program itself, you may want to visit the Viseur Home page: http://www.lctn.u-nancy.fr/viseur/viseur.html PS: I'm reading this newsgroup via the newsgroup interface but would welcome its moderation. Fabien Campagne -- campagne@incm.u-nancy.fr | Lab. de Chimie Theorique phone: +33 (0)3 83 91 20 00 extension 3236 | Nancy, France. http://www.lctn.u-nancy.fr/viseur/FC.html From owner-7tms_r@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!VIOLET.INCM.U-NANCY.FR!poda From: poda@VIOLET.INCM.U-NANCY.FR (Gennady PODA) Newsgroups: bionet.molbio.proteins.7tms_r Subject: A voice for moderation Date: 13 May 1997 11:12:58 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net full support for the moderation of the newsgroup. ************************************************************ Dr. Gennady PODA Laboratoire de Chimie theorique (UA 510 du C.N.R.S.) Universite Henri Poincare, Nancy-I Faculte des Sciences - Domaine scientifique Victor Grignard B.P. 239 - 54500 Vandoeuvre-les-Nancy Cedex France Fax : + 33 (0)3.83.91.25.30 E-mail : poda@incm.u-nancy.fr ************************************************************ From owner-7tms_r@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!FERRE.SACLAY.CEA.FR!nicolas From: nicolas@FERRE.SACLAY.CEA.FR (Nicolas Ancellin) Newsgroups: bionet.molbio.proteins.7tms_r Subject: moderation Date: 13 May 1997 08:58:42 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net OK for moderation ------------------------------------------------------------------------------- Nicolas Ancellin Departement de Biologie Cellulaire et Moleculaire Service de Biologie Cellulaire Bat 520 p2, CEA CEN-Saclay F91191 Gif sur Yvette Cedex, France Tel (33) 01 69 08 36 27 fax (33) 01 69 08 35 70 nicolas@ferre.saclay.cea.fr -------------------------------------------------------------------------------- From owner-7tms_r@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!MIDWAY.UCHICAGO.EDU!khouamed From: khouamed@MIDWAY.UCHICAGO.EDU (Khaled Houamed) Newsgroups: bionet.molbio.proteins.7tms_r Subject: MOLECULAR NEUROBIOLOGY POSTDOCS U. CHICAGO Date: 13 May 1997 09:19:18 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 53 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Postdoctoral Positions in Molecular Neurobiology University of Chicago Postdoctoral positions are available to: 1) Perform structure / function studies on recombinant small conductance calcium activated potassium channels (SK channels) and, 2) Perform structure / function studies on recombinant G-protein coupled receptors members of family C (metabotropic glutamate receptors, calcium sensor, and GABA subB receptor). Recent Ph.D. graduates in Physiology, Pharmacology, Biochemistry, Neuroscience, or allied fields, with experience in cell physiology (patch clamp, oocyte voltage clamp, or fura measurements), or molecular biology techniques are invited to apply for these positions. Please send a current CV including a summary of relevant research experience and names and addresses of three referees to Dr. K. Houamed at the address below. Khaled Houamed Ph.D. Department of Anesthesia and Critical Care, Committees on Neurobiology and Cell Physiology The University of Chicago Medical Center 5841 S. Maryland Ave., Box 4028 Chicago, IL 60637 USA Tel # Secretary (773) 702-4055 Office (773) 702-7552 Laboratory (773) 834-0385 Fax # (773) 702-4791 The University of Chicago is an Affirmative Action, Equal Opportunity Employer. From owner-7tms_r@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!BIOFIS.EPM.BR!ACMPAIVA From: ACMPAIVA@BIOFIS.EPM.BR ("Antonio C. M. Paiva") Newsgroups: bionet.molbio.proteins.7tms_r Subject: moderate Date: 13 May 1997 07:56:27 -0700 Organization: ESCOLA PAULISTA DE MEDICINA Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <4C4BAE441CD@BIOFIS.EPM.BR> Reply-To: acmpaiva@BIOFIS.EPM.BR NNTP-Posting-Host: net.bio.net Vote for moderate From owner-7tms_r@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!yorvic.york.ac.uk!pavel From: pavel@yorvic.york.ac.uk (Pawel Herzyk) Newsgroups: bionet.molbio.proteins.7tms_r Subject: moderate Date: 13 May 1997 08:58:42 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <9705131659.ZM25385@wilma> NNTP-Posting-Host: net.bio.net full suport for moderation of this newsgroup -- Dr Pawel Herzyk pavel@yorvic.york.ac.uk Department of Chemistry tel. (+44) 01904 432593 University of York fax. (+44) 01904 410519 Heslington, YORK, YO1 5DD, UK. http://www.yorvic.york.ac.uk/~pavel From owner-7tms_r@net.bio.net Tue May 13 23:00:00 1997 Path: biosci!CHAGALL.GALAXY.IBPC.FR!cathy From: cathy@CHAGALL.GALAXY.IBPC.FR (cathy ETCHEBEST) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Moderation Date: 14 May 1997 00:23:04 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net OK for Moderation Thank you Catherine ETCHEBEST Laboratoire de Biochimie Theorique I.B.P.C UPR9080 13 rue Pierre et Marie Curie 75005 Paris Tel 01-43-25-26-09 poste 397 Fax 01-43-29-56-45 EMail:cathy@ibpc.fr From owner-7tms_r@net.bio.net Tue May 13 23:00:00 1997 Path: biosci!mail.nrgn.com!rbrodbeck From: rbrodbeck@mail.nrgn.com ("Robbin Brodbeck") Newsgroups: bionet.molbio.proteins.7tms_r Subject: moderation policy Date: 14 May 1997 05:36:11 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net With respect to the moderation policy suggested by Frank: Moderation Policy: Mass-posted commercial messages, chain letters, and similar postings not germane to plants or signal transduction will be deleted without comment. Inappropriate messages posted in good faith will be returned to the sender. Messages not strictly within the charter but likely to be of interest to many subscribers (e.g., messages dealing with certain aspects of signal transduction in animal systems) will be accepted. Use of the newsgroup for commercial purposes is prohibited. Many people might agree that certain "commercial" postings with regard to reagents/ software / kits, etc which are novel &/or upgraded, and are of interest to much of the group, might be considered valuble, therefore even though commercial in nature, should be freely transmitted. We've only seen a minimal amount of this type of posting in the past, but I think it would be an added bonus sincerly, a scientist who doesn't have much time to go out shopping for new science toys. From owner-7tms_r@net.bio.net Tue May 13 23:00:00 1997 Path: biosci!LILLY.COM!SHARP_JOHN_D From: SHARP_JOHN_D@LILLY.COM ("J.D.SHARP 317-276-4268 DROP 04") Newsgroups: bionet.molbio.proteins.7tms_r Subject: No Brainer Date: 14 May 1997 11:22:01 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <6526211314051997.A87934.MCVAX4.11B573522800*@MHS> NNTP-Posting-Host: net.bio.net People, I hate "modern" terms like "no brainer" however in this case it is imminently applicable: MODERATE THIS GROUP AND KICK AWAY THE FLUFF! More power to people like Frank who volunteers and Gert who frequently pitches in! ______________________________________________________________________ FOOT FOOT FOOT FOOT] John D. Sharp O O F O F F Lilly Research 0434 OOT O O O O O Indianapolis, IN 46285 T T O T O O sharp_john_d@lilly.com | FOOT FOOT T 317-276-4268 ______________________________________________________________________ From owner-7tms_r@net.bio.net Tue May 13 23:00:00 1997 Path: biosci!daresbury!uninett.no!stdio!news-spur1.maxwell.syr.edu!news.maxwell.syr.edu!cs.utexas.edu!geraldo.cc.utexas.edu!netnews.uthscsa.edu!kolakowski.uthscsa.edu!kolakowski From: Frank Kolakowski Newsgroups: bionet.molbio.proteins.7tms_r Subject: Revised Charter for 7TMS_R Newsgroup/Mailing List Date: 14 May 1997 16:11:45 GMT Organization: Dept. of Pharmacology - UTHSCSA Lines: 71 Distribution: world Message-ID: <5lco81$ql7$1@cosmos.uthscsa.edu> NNTP-Posting-Host: kolakowski.uthscsa.edu Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Newsreader: Nuntius 2.0.4_PPC X-XXMessage-ID: X-XXDate: Wed, 14 May 1997 16:04:54 GMT 7TMS_R Readers/Posters: I have revised the charter to add the moderation rules. Several people have suggested that a complete ban on commercial postings might be limiting the free exchange of information relevant to the newsgroup. I have added language that will allow commercial postings provided they are informational in content and relevant to our newsgroup's topic. Please feel free to comment on the charter for a short while. I will add/revise and send to BIONET on Friday. Frank ------------------------------------------------------------------------- Information for G-PROTEIN-COUPLED-RECEPTOR/bionet.molbio.proteins.7tms_r USENET name: bionet.molbio.proteins.7tms_r Mailing list name: G-PROTEIN-COUPLED-RECEPTOR Status: Moderated One line Description: Research on G-protein coupled receptor systems E-mail addresses: 7tms_r@net.bio.net 7tms_r@daresbury.ac.uk Discussion leader: Lee F. Kolakowski, Jr., Ph.D. Email: kolakowski@uthscsa.edu Department of Pharmacology University of Texas Health Science Center at San Antonio 7703 Floyd Curl Drive San Antonio, Texas 78284-7764 Phone or Fax: 210-567-4233 (Office) 210-567-4178 (Lab) 210-567-4303 (Fax) Charter: The purpose of G-PROTEIN-COUPLED-RECEPTOR/bionet.molbio.proteins.7tms_r is to function as a forum for discussion for researchers involved in the cloning, characterization and analysis of members of the super-family of heptahelical signal transducing receptors which interact with heterotrimeric G-proteins. Associated topics of molecular pharmacology, protein structure/function, characterization of orphan receptors, and other questions or discussions revolving around these proteins are also germane. The newsgroup will facilitate exchange of information, protocols, ideas and function as a forum for discussion. Subscribers are welcome from universities or any academic institutions, government agencies, medical institutions, and industrial or commercial organizations. Contributions within the functions outlined above are encouraged. Moderation Policy: Mass-posted commercial messages, chain letters, and similar postings not germane to G-protein coupled receptors or signal transduction will be deleted without comment. Inappropriate messages posted in good faith will be returned to the sender. Unprofessional messages of any kind will be deleted without comment. Messages not strictly within the charter but likely to be of interest to many subscribers (e.g., messages describing non G-protein mediated signaling) will be accepted. Messages of commercial content relevant to G-protein coupled receptor systems will be allowed providing the details of the system and the relevance to the newsgroup are the focus of the messages. Use of the newsgroup for commercial purposes is prohibited (except as outlined above at the discretion of the moderator). From owner-7tms_r@net.bio.net Tue May 13 23:00:00 1997 Path: biosci!EMBL-HEIDELBERG.DE!Gert.Vriend From: Gert.Vriend@EMBL-HEIDELBERG.DE Newsgroups: bionet.molbio.proteins.7tms_r Subject: moderation and commerce Date: 14 May 1997 06:37:44 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705141338.PAA02331@nu> NNTP-Posting-Host: net.bio.net The GPCRDB has space reserved for commercial messages. We made this known to about 30 chemical firms who had the word GPCR somewhere in their WWW pages. They all got offered free space on this server. We got zero answers. I therefore suggest that we strickly ban all commercial messages, and if the moderator considers them generally useful he can tell them to hand it to us to be put on the GPCRDB. Look at http://swift.embl-heidelberg.de/7tm/other/industry_adds.html to see the still informationless industry page.... Gert Vriend From owner-7tms_r@net.bio.net Tue May 13 23:00:00 1997 Path: biosci!YALE.EDU!henrik.dohlman From: henrik.dohlman@YALE.EDU (Henrik Dohlman) Newsgroups: bionet.molbio.proteins.7tms_r Subject: moderation policy Date: 14 May 1997 06:34:43 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 43 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I agree with Robbin that commercial postings can be useful, but they are often disguised as some bench scientist passing along a tip about some new product to his webmates. Commercial postings should be clearly labeled as such. Another issue to consider is whether we should permit messages meant only to publicly berate a colleague, such as that suffered by the hapless Dr. Rao not long ago. I'm all for free speech but I thought the postings were hardly "germane". Henrik Robbin Brodbeck wrote: > >With respect to the moderation policy suggested by Frank: > >Moderation Policy: Mass-posted commercial messages, chain letters, and >similar postings not germane to plants or signal transduction will be >deleted without comment. Inappropriate messages posted in good faith will >be returned to the sender. Messages not strictly within the charter but >likely to be of interest to many subscribers (e.g., messages dealing with >certain aspects of signal transduction in animal systems) will be >accepted. >Use of the newsgroup for commercial purposes is prohibited. > >Many people might agree that certain "commercial" postings with regard to >reagents/ software / kits, etc which are novel &/or upgraded, and are of >interest to much of the group, might be considered valuble, therefore even >though commercial in nature, should be freely transmitted. > >We've only seen a minimal amount of this type of posting in the past, but I >think it would be an added bonus > >sincerly, > >a scientist who doesn't have much time to go out shopping for new science >toys. > Henrik G. Dohlman, PhD Yale University School of Medicine henrik.dohlman@yale.edu From owner-7tms_r@net.bio.net Tue May 13 23:00:00 1997 Path: biosci!merck.de!Friedrich.Rippmann From: Friedrich.Rippmann@merck.de (Friedrich Rippmann) Newsgroups: bionet.molbio.proteins.7tms_r Subject: moderation Date: 14 May 1997 06:04:00 -0700 Organization: Merck KGaA Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <3379B848.31DF@popserver.dd.merck.de> NNTP-Posting-Host: net.bio.net I vote for moderation. However, my feeling is that the guidelines should not be too strict. Relevant commercial postings (innovations) should be allowed. Job postings should be allowed as well, both from academic and commercial institutions. Just my opinion, - Friedrich - -------------------------------------------------------------------- Dr. Friedrich Rippmann work: home: c/o MERCK KGaA Schroederstrasse 79 Med. Chemistry/Bio- and Chemoinformatics 69120 Heidelberg 64271 Darmstadt, Germany Tel/Fax +49-6221-413366 Tel +49-6151-726290 Email: rippmann@merck.de Fax ... 723329 -------------------------------------------------------------------- From owner-7tms_r@net.bio.net Fri May 16 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!ix.netcom.com!news From: ERIC Newsgroups: bionet.molbio.proteins.7tms_r Subject: Research on wearable air purifier Date: Sun, 18 May 1997 00:11:07 GMT Organization: Netcom Lines: 19 Message-ID: <5llhhl$t5p@sjx-ixn5.ix.netcom.com> NNTP-Posting-Host: lbx-ca20-11.ix.netcom.com X-NETCOM-Date: Sat May 17 5:12:37 PM PDT 1997 X-Newsreader: NETCOMplete/3.27 My name is Eric and I am doing research at UCLA on this portable air purifier. It is called the Air Supply. My studies have shown it is capable of reducing bacteria directed towards a target plate by 90% or more. When worn, this device is useful in protecting people from viruses, bacteria and other allergens. This can be very useful to those who are immunocompromised It relies on Corona Discharge Technology, which is similar to the effect a lightning bolt has on it's environment when it strikes. We are in the process of publishing our data. Also, can someone give me information on the effects of negative ions as opposed to positive ions? Thanks For more information on the Air Supply, goto http://www.breathefree.com From owner-7tms_r@net.bio.net Mon May 19 23:00:00 1997 Path: biosci!CHEMPATH.UCT.AC.ZA!DAVID From: DAVID@CHEMPATH.UCT.AC.ZA ("Dave") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Moderation & Agonist binding Date: 20 May 1997 22:16:58 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I was just interested as to when moderation was supposed to start, as all I have seen for the past week in this group is SPAM mail. As a service to others, I have reported several of the spammers to their relevant postmasters. However, its kind of like the "kill one, 1000 more appear" scenario. And now for some work: Does anyone know what differences exist between agonist binding done at 4 C and 37 C? As far as I know, most people do agonist binding at 4 C to avoid receptor activation and the associated structural/affinity changes. Do you all agree/disagree with this reasoning? Thanks -------------------------------------------------------------------- Dave Myburgh Lab 227 Falmouth Bldg E-mail: david@chempath.uct.ac.za Dept. Chemical Pathology mybdav02@uctvms.uct.ac.za UCT Medical School Phone: 406-6359 Observatory FAX: 448-8150 7925 South Africa -------------------------------------------------------------------- From owner-7tms_r@net.bio.net Tue May 20 23:00:00 1997 Path: biosci!MAIL.MED.CORNELL.EDU!mjcann From: mjcann@MAIL.MED.CORNELL.EDU (Martin Cann) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Immunocytochemistry Date: 21 May 1997 12:18:06 -0700 Organization: Cornell University Medical College Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <338358AD.3ED9@mail.med.cornell.edu> NNTP-Posting-Host: net.bio.net I want to do immunocytochemistry of an integral membrane protein on whole Drosophila embryos. I am concerning about epitope masking with aldehyde fixation and antigen extraction during the procedure. Does anyone have any experience? What are the fixatives? What about fixative osmolarity? Detergent or no detergent? Please e-mail me at mjcann@med.cornell.edu Martin Cann From owner-7tms_r@net.bio.net Tue May 20 23:00:00 1997 Path: biosci!GEMS.VCU.EDU!rpuffenbarg From: rpuffenbarg@GEMS.VCU.EDU (Robyn Puffenbarger) Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe Date: 21 May 1997 11:43:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net subscribe rpuffenbarg@gems.vcu.edu From owner-7tms_r@net.bio.net Tue May 20 23:00:00 1997 Path: biosci!agate!howland.erols.net!newsfeed.internetmci.com!portal.gmu.edu!solaris.cc.vt.edu!newsgate.duke.edu!kwatra2.mc.duke.edu!user From: edroush@acpub.duke.edu (Eric Roush) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Moderation & Agonist binding Date: Wed, 21 May 1997 10:27:26 -0400 Organization: Glow-n-the-Dark Radiochemicals, Inc. Lines: 26 Distribution: world Message-ID: References: NNTP-Posting-Host: kwatra2.mc.duke.edu In article , DAVID@CHEMPATH.UCT.AC.ZA ("Dave") wrote: > I was just interested as to when moderation was supposed to start, as > all I have seen for the past week in this group is SPAM mail. As a > service to others, I have reported several of the spammers to their > relevant postmasters. However, its kind of like the "kill one, 1000 > more appear" scenario. > > And now for some work: Does anyone know what differences exist > between agonist binding done at 4 C and 37 C? As far as I know, most > people do agonist binding at 4 C to avoid receptor activation and the > associated structural/affinity changes. Do you all agree/disagree > with this reasoning? Well, if you are examining binding in vivo, then 4 °C (or better yet, on ice at 0 °C) shuts down endocytosis, ensuring that the surface receptor population remains constant during the experiment. -- Eric Roush When an eel edroush@acpub.duke.edu bites your heel, also coache@aol.com makes you squeal, pain you feel, that's a moray. From owner-7tms_r@net.bio.net Tue May 20 23:00:00 1997 Path: biosci!PO.CWRU.EDU!pre From: pre@PO.CWRU.EDU ("Paul Ernsberger, Ph.D.") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Moderation & Agonist binding Date: 21 May 1997 18:36:54 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705220137.VAA02125@cornelius.INS.CWRU.Edu> NNTP-Posting-Host: net.bio.net At 10:16 PM 5/20/97 -0700, Dave wrote: > >And now for some work: Does anyone know what differences exist >between agonist binding done at 4 C and 37 C? As far as I know, most >people do agonist binding at 4 C to avoid receptor activation and the >associated structural/affinity changes. Do you all agree/disagree >with this reasoning? > Because membrane lipids are essentially solid at 4oC, you have a very different situation. Temperature can affect density, affinity and rank order of potency in ways that are not always predictable. Lipophilic ligands will often give you high nonspecific at 4oC. Paul Ernsberger, Ph.D., Associate Professor of Medicine, Pharmacology and Neuroscience Case Western Reserve University School of Medicine, Cleveland, OH 44106-4982 USA E-mail: pre@po.cwru.edu FAX: (216)368-4752 From owner-7tms_r@net.bio.net Tue May 20 23:00:00 1997 Path: biosci!PO.CWRU.EDU!pre From: pre@PO.CWRU.EDU ("Paul Ernsberger, Ph.D.") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Immunocytochemistry Date: 21 May 1997 18:51:33 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705220152.VAA03305@cornelius.INS.CWRU.Edu> NNTP-Posting-Host: net.bio.net At 12:18 PM 5/21/97 -0700, Martin Cann wrote: >I want to do immunocytochemistry of an integral membrane protein on >whole Drosophila embryos. I am concerning about epitope masking with >aldehyde fixation and antigen extraction during the procedure. Does >anyone have any experience? What are the fixatives? What about >fixative osmolarity? Detergent or no detergent? > I am surprised to see this question from Cornell! Ask Ginny Pickel or Terry Milner in the Kips Bay building--they have a cookbook of fixation recipes for integral membrane proteins. Paul Ernsberger, Ph.D., Associate Professor of Medicine, Pharmacology and Neuroscience Case Western Reserve University School of Medicine, Cleveland, OH 44106-4982 USA E-mail: pre@po.cwru.edu FAX: (216)368-4752 From owner-7tms_r@net.bio.net Thu May 22 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!howland.erols.net!newsxfer.itd.umich.edu!newbabylon.rs.itd.umich.edu!not-for-mail From: Rick Neubig Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Moderation & Agonist binding Date: Fri, 23 May 1997 17:37:59 -0400 Organization: University of Michigan Lines: 52 Message-ID: <33860E37.5E517C9@umich.edu> References: NNTP-Posting-Host: warbler.med.umich.edu Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 X-Mailer: Mozilla 4.0b4 [en] (Win95; I) To: Dave X-Priority: 3 (Normal) Dave, I'd say that most studies are done at low temperatures to avoid receptor or ligand degradation (esp for peptide ligands). There are numerous studies of ligand binding to GPCR's at different temperatures. Perry Molinoff started work on this type of study to examine thermodynamics of agonist vs antagonist binding back in the early 80's or so. We published one study looking at alpha2AR-G protein coupling at different temperatures: Gantzos, R.D. and Neubig, R.R. Temperature effects on a2-adrenergic receptor-Gi interactions. Biochem.Pharmacol. 37:2815-2821, 1988. What you see will depend a lot on whether you are studying binding in whole cells or membranes. In the former the low temperatures will disrupt a lot of processes that don't occur in membranes such as internalization and receptor downregulation. Did you have something specific that you were wondering about in terms of effects? Hope this helps. Rick Dave wrote: > (snip) > > And now for some work: Does anyone know what differences exist > between agonist binding done at 4 C and 37 C? As far as I know, most > people do agonist binding at 4 C to avoid receptor activation and the > associated structural/affinity changes. Do you all agree/disagree > with this reasoning? > > Thanks > > -------------------------------------------------------------------- > Dave Myburgh > Lab 227 Falmouth Bldg E-mail: david@chempath.uct.ac.za > Dept. Chemical Pathology mybdav02@uctvms.uct.ac.za > UCT Medical School Phone: 406-6359 > Observatory FAX: 448-8150 > 7925 > South Africa > -------------------------------------------------------------------- -- Rick Neubig RNeubig@umich.edu Department of Pharmacology University of Michigan Phone (313) 763-3650 FAX (313) 763-4450 http://www-personal.umich.edu/~rneubig/ From owner-7tms_r@net.bio.net Mon May 26 23:00:00 1997 Path: biosci!anatomy.emory.edu!karl From: karl@anatomy.emory.edu (Karl saxe) Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscsribe Date: 27 May 1997 13:20:40 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <338B0A9D.C3E@anatomy.emory.edu> NNTP-Posting-Host: net.bio.net subscribe 7TMS_R From owner-7tms_r@net.bio.net Mon May 26 23:00:00 1997 Path: biosci!monsanto.com!JMSTAU From: JMSTAU@monsanto.com Newsgroups: bionet.molbio.proteins.7tms_r Subject: unsubscribe Date: 27 May 1997 15:19:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <0030400003957901000002*@MHS> NNTP-Posting-Host: net.bio.net unsubscribe From owner-7tms_r@net.bio.net Tue May 27 23:00:00 1997 Path: biosci!ZOO.UVM.EDU!tspaldin From: tspaldin@ZOO.UVM.EDU ("Tracy A. Spalding") Newsgroups: bionet.molbio.proteins.7tms_r Subject: ISIS and other database programs Date: 28 May 1997 16:57:09 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I am looking for information concerning large database management programs that can handle millions of chemical structures coupled with EC50 / efficacy data and high-throughput screening data for a number of different receptors. We are considering going with MDL's ISIS software package. Are there any ISIS users out there who have strong views about this program or any competing products from other companies? As always, I will summarize any responses that I get and post them back to the newsgroup. Yours Tracy Spalding tspaldin@moose.uvm.edu From owner-7tms_r@net.bio.net Wed May 28 23:00:00 1997 Path: biosci!MSMAIL.BMS.COM!Wiener#m#_Harvey_L#d#.WFD_Mail_Server From: Wiener#m#_Harvey_L#d#.WFD_Mail_Server@MSMAIL.BMS.COM ("Wiener, Harvey L.") Newsgroups: bionet.molbio.proteins.7tms_r Subject: RE: ISIS and other database programs Date: 29 May 1997 06:28:48 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net On Wed, May 28, 1997 8:10 PM, Tracy A. Spalding (tspaldin@moose.uvm.edu) wrote: >I am looking for information concerning large database management programs >that can handle millions of chemical structures coupled with EC50 / efficacy >data and high-throughput screening data for a number of different >receptors. We are considering going with MDL's ISIS software package. >Are there any ISIS users out there who have strong views about this >program or any competing products from other companies? My experience with MDL's ISIS software to query Oracle databases has been quite favorable. Their training was excellent and the program has drawing and retreiving modules which allow for sub-structure searching and the rapid generation of structure-activity relationship tables. I find that its a fine tool to retrieve chemical and biological data. Of course you will also need to select or develop a software package capable of rapidly estimating EC50 and efficacy values for such a large number of compounds in the high-throughput screening environment that also transfers these data into an Oracle database. This should be an equally important consideration for a receptor pharmacologist. Harvey L. Wiener, Ph.D. Department of Neurochemistry Neuroscience Drug Discovery Bristol-Myers Squibb Co. Wallingford, CT Wiener#m#_Harvey_L#d#@msmail.bms.com From owner-7tms_r@net.bio.net Wed May 28 23:00:00 1997 Path: biosci!PUBLIC.CQ.SC.CN!ygzhou From: ygzhou@PUBLIC.CQ.SC.CN (YuanGuo Zhou) Newsgroups: bionet.molbio.proteins.7tms_r Subject: looking for a protocol for steroid receptor binding analysis Date: 29 May 1997 17:06:52 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705292307.IAA00982@public.cq.sc.cn> NNTP-Posting-Host: net.bio.net Hi, I have some problems with the steroid receptors binding analysis with 3H-dexamethasone. Does anybody have the protocol? I like to get one. Thanks Response via e-mail please. Rick Zhou From owner-7tms_r@net.bio.net Thu May 29 23:00:00 1997 Newsgroups: bionet.molbio.proteins.7tms_r Path: biosci!agate!howland.erols.net!worldnet.att.net!newsfeed.internetmci.com!rill.news.pipex.net!pipex!server1.netnews.ja.net!warwick!bris.ac.uk!usenet From: Klaus Jandt Subject: PhD Studentship X-Nntp-Posting-Host: restkj.dent.bris.ac.uk Content-Type: text/plain; charset=iso-8859-1 Message-ID: <338F4395.221A@bris.ac.uk> Sender: usenet@fsa.bris.ac.uk (Usenet) Content-Transfer-Encoding: 8bit Organization: Universitry of Bristol, Department of Oral and Dental Science Mime-Version: 1.0 Date: Fri, 30 May 1997 21:16:05 GMT X-Mailer: Mozilla 2.01 (Win95; I; 16bit) Lines: 59 The Biomaterials Science Group Department of Oral and Dental Science in co-operation with the Physics Department University of Bristol Lower Maudlin Street, Bristol, BS1 2LY, England For the project "Protein-Biomaterials-Interfaces Investigated with Atomic Force Microscopy" we are looking for a PhD Student with a background in Physics or Materials Science or Chemistry or Biology or Engineering or a student of related areas. We expect: · EU citizenship (non-EU citizens can be considered if they provide funding which covers the difference between the oversees fee and the home fee) · Upper Second Class degree or better · Enthusiasm for the work in the area of biomaterials interfaces · Eagerness to explore new areas, initiative and result oriented work · Starting date 1st October 1997 · Open, cooperative character for the work in an international team We offer: · Research at one of the leading British research universities in attractive surroundings · Top departments · PhD fees are paid · Maintenance and travel grant (co-operation with a university in California possible) · Applied scientific research on a high level · Intensive supervision · Work in international teams with an excellent working atmosphere · Future oriented research area · Premium (£ 2000) from industrial sponsor paid upon successful completion of PhD research programme Closing date for applications 15th June 1997 Applications should be submitted to: Dr. Klaus D. Jandt Senior Lecturer Dental Materials Science and Biomaterials University of Bristol Department of Oral and Dental Science Lower Maudlin Street Bristol, BS1 2LY, UK Phone: ++ 44 (0)117 9 28 44 18 Internet: K.Jandt@bris.ac.uk