From owner-7tms_r@net.bio.net Tue Jul 01 23:00:00 1997 Path: biosci!agate!spool.mu.edu!uwm.edu!vixen.cso.uiuc.edu!howland.erols.net!newsxfer3.itd.umich.edu!newbabylon.rs.itd.umich.edu!not-for-mail From: Rick Neubig Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Moderate or unsubscribe?? Date: Wed, 02 Jul 1997 08:27:08 -0400 Organization: University of Michigan Lines: 23 Message-ID: <33BA491C.6FAB8BA2@umich.edu> References: <33B8DEE4.32F4@qub.ac.uk.see.signature> NNTP-Posting-Host: warbler.med.umich.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.01 [en] (Win95; U) X-Priority: 3 (Normal) One major question about moderation is who is going to do it? I haven't been keeping up with the group so I didn't see the discussion. Did someone volunteer to moderate the group? Rick Neubig RNeubig@umich.edu Department of Pharmacology University of Michigan Phone (313) 763-3650 FAX (313) 763-4450 http://www-personal.umich.edu/~rneubig/ Andrew Wallace wrote: > David A. Schooley wrote: > > > > I thought a decision had been made to moderate > communications about > > a month ago. Since then, it seems the frequency of unsolicited > mail has > > only increased, until it greatly exceeds the scientific > communications. > From owner-7tms_r@net.bio.net Tue Jul 01 23:00:00 1997 Path: biosci!glaxowellcome.com!rc7694 From: rc7694@glaxowellcome.com Newsgroups: bionet.molbio.proteins.7tms_r Subject: CRF & VIP Date: 2 Jul 1997 06:40:40 -0700 Organization: Glaxo Wellcome Inc. Lines: 4 Sender: daemon@net.bio.net Distribution: world Message-ID: <33BA5CFF.5879@glaxo.com> NNTP-Posting-Host: net.bio.net Does anyone know if CRF can interact with VIP receptors? Rich Cox Glaxo Wellcome, RTP, USA From owner-7tms_r@net.bio.net Wed Jul 02 23:00:00 1997 Path: biosci!ALCOR.BMS.COM!krystek From: krystek@ALCOR.BMS.COM (Stanley Krystek) Newsgroups: bionet.molbio.proteins.7tms_r Subject: moderate Date: 3 Jul 1997 12:05:30 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <9707031906.AA25332@alcor.bms.com> NNTP-Posting-Host: net.bio.net I thought we decided for moderation, I am sure we all find the extra traffic of poor taste. stan Stanley R. Krystek, Jr., Ph.D. Bristol-Myers Squibb Pharmaceutical Research Institute Department of Macromolecular Modeling P.O. Box 4000, Room H.3812, Princeton, N.J. 08543-4000 (609) 252-4100 FAX: (609) 252-6030 INTERNET: krystek@bms.com From owner-7tms_r@net.bio.net Wed Jul 02 23:00:00 1997 Path: biosci!agate!howland.erols.net!europa.clark.net!newsfeed.direct.ca!news.he.net!news.rain.net!news.teleport.com!not-for-mail From: nevek@teleport.com (Kim Neve) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Moderate or unsubscribe?? Date: Thu, 03 Jul 1997 14:56:49 GMT Organization: Oregon Health Sciences University Lines: 16 Message-ID: <33bbbcef.481243631@news.teleport.com> References: <33B8DEE4.32F4@qub.ac.uk.see.signature> <33BA491C.6FAB8BA2@umich.edu> NNTP-Posting-Host: ip-pdx07-06.teleport.com X-Newsreader: Forte Free Agent 1.1/32.230 Rick Neubig wrote: >One major question about moderation is who is going to do it? I haven't >been keeping up with the group so I didn't see the discussion. Did >someone volunteer to moderate the group? Rick, When the original discussion on moderation took place, it was my understanding that Frank was offering to be the moderator. We have not heard from Frank recently on this or any other topic, so perhaps an email message to him would be in order. More recently, you may have seen Gert Vriend's offer to moderate if Frank did not have the time. Kim From owner-7tms_r@net.bio.net Wed Jul 02 23:00:00 1997 Path: biosci!daresbury!server5.netnews.ja.net!server6.netnews.ja.net!server1.netnews.ja.net!rill.news.pipex.net!pipex!join.news.pipex.net!pipex!oleane!jussieu.fr!univ-lille1.fr!ciril.fr!not-for-mail From: Fabien Campagne Newsgroups: bionet.molbio.proteins.7tms_r Subject: Alignment and Snake-like HTML WWW services are available Date: Thu, 03 Jul 1997 12:58:56 +0100 Organization: Laboratoire de Chimie The'orique de Nancy Lines: 29 Message-ID: <33BB9400.2781@incm.u-nancy.fr> NNTP-Posting-Host: yellow.incm.u-nancy.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0SC-SGI (X11; I; IRIX64 6.2 IP28) We now propose WWW online services that may be of interest to you. http://www.lctn.u-nancy.fr/viseur/services.html * One takes SWISSSPROT sequences snake-like according to user TM limit choice. (illustration: http://www.lctn.u-nancy.fr/viseur/snake-like-view/OPRD_HUMAN.html) * The second one takes MSF alignments as input and create HTML and configurable PostScript alignments for use on WWW servers or view locally via a WWW browser. This service uses a new method to create HTML alignments that enables a user to browse several color alignments in a fast way (really fast for UNIX clients: the data is sent compressed on the net, data transfer is usually less than 1ko per seq. in the alignment). The users may wait for the first alignment to transfer through the web but getting second and subsequent alignments is REALLY fast (the method uses the browser image cache intensively). (illustration: http://www.lctn.u-nancy.fr/viseur/services/align.html for UNIX systems: http://www.lctn.u-nancy.fr/viseur/services/alignc.html.Z) Comments and suggestions are welcomed. Fabien Campagne -- campagne@incm.u-nancy.fr | Lab. de Chimie Theorique phone: +33 (0)3 83 91 20 00 extension 3236 | Nancy, France. http://www.lctn.u-nancy.fr/viseur/viseur.html http://www.lctn.u-nancy.fr/viseur/FC.html From owner-7tms_r@net.bio.net Thu Jul 03 23:00:00 1997 Path: biosci!webtv.net!uunet!in1.uu.net!160.45.4.4!fu-berlin.de!news-ber1.dfn.de!news-lei1.dfn.de!news-nue1.dfn.de!uni-erlangen.de!winx03!wpxx02!not-for-mail From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Messages? Date: Sun, 22 Jun 1997 20:55:37 +0200 Organization: University of Wuerzburg, Germany Lines: 11 Message-ID: <9fsjo5.04p.ln@wpxx02.toxi.uni-wuerzburg.de> NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Did anyone get messages on this list since the newsgroup started to become moderated? Would injecting newgrps at different points through usenet help? --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP3 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-7tms_r@net.bio.net Thu Jul 03 23:00:00 1997 Path: biosci!agate!hammer.uoregon.edu!vixen.cso.uiuc.edu!howland.erols.net!newsfeed.nacamar.de!wuff.mayn.de!wuff.franken.de!news-nue1.dfn.de!uni-erlangen.de!winx03!wpxx02!not-for-mail From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Moderate or unsubscribe?? Date: Sat, 28 Jun 1997 17:48:18 +0200 Organization: University of Wuerzburg, Germany Lines: 23 Distribution: world Message-ID: <2ob3p5.pp2.ln@wpxx02.toxi.uni-wuerzburg.de> References: NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] David A. Schooley (schooley@UNR.EDU) wrote: > I thought a decision had been made to moderate communications about > a month ago. Since then, it seems the frequency of unsolicited mail has > only increased, until it greatly exceeds the scientific communications. > I have already seen a colleague from another department here > unsubscribe. Is moderation really going to happen? IMO there are at least two problems: - propagation of b.m.p.7tms_r is lousy - it is not clear whether the group is already moderated or not (even if it was, it could still be that the control messages are propagated as poorly as the rest of the group, making moderation ineffective) It may very well be that Frank, who has volunteered for moderation, may never see this message at all :-( --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP3 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-7tms_r@net.bio.net Sat Jul 05 23:00:00 1997 Path: biosci!USA.NET!liangyichen From: liangyichen@USA.NET ("liangyichen") Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 6 Jul 1997 17:22:59 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <19970707002638.24477.qmail@dns01.ops.usa.net> NNTP-Posting-Host: net.bio.net --19930302094736PvzbhnRfO8m40nvG Content-Type: text/plain; charset="US-ASCII" Content-Transfer-Encoding: 7bit Subscribe liangyichen@usa.net --19930302094736PvzbhnRfO8m40nvG-- From owner-7tms_r@net.bio.net Mon Jul 07 23:00:00 1997 From: Damn Yankee Newsgroups: bionet.molbio.proteins.7tms_r Organization: Yankee Inc. Subject: !!!Hello!!! NNTP-Posting-Host: 205.139.183.49 Message-ID: <33c0b447.0@nntp.kalnet.net> Date: 7 Jul 97 09:17:59 GMT Lines: 9 Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed4.bbnplanet.com!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!pull-feed.internetmci.com!nntp.kalnet.net!205.139.183.49 Are you looking for a great place to find interesting things on the WWW??? Than look no more because you have found the right place!!! The place you are looking for is Yankee Inc.!!! A great Web Site full of interesting links to interesting places!!! Everything from Adult to Xzlyaphone it's here!!! There is something for everyone and then some!!! So give it a try what do you have to lose except some sleep??? Yankee Inc. http://www.kalnet.net/yank714 Yankee Inc. Your Alternative Web Solution!!! Yankee Inc. http://www.kalnet.net/yank714 If you would like to be removed from my mailing list - hit reply and type "REMOVE" and I will promptly remove you from my list!!! Thank You!!! From owner-7tms_r@net.bio.net Tue Jul 08 23:00:00 1997 From: Damn Yankee Newsgroups: bionet.molbio.proteins.7tms_r Organization: Yankee Inc. Subject: I Am Very Sorry!!! NNTP-Posting-Host: 205.139.183.55 Message-ID: <33c2555f.2@nntp.kalnet.net> Date: 8 Jul 97 14:57:35 GMT Lines: 5 Path: biosci!rutgers!gatech!howland.erols.net!newsfeed.internetmci.com!pull-feed.internetmci.com!nntp.kalnet.net!205.139.183.55 I would like to apologise to this newsgroup and everyone who reads this newsgroup!!! I promise never to post or send spam to this or any other newsgroup that does not pertain to my posting!!! Please accept my humble apology and again I will never post spam here again!!! Thank You!!! Andrew Schero yank714@kalnet.net From owner-7tms_r@net.bio.net Wed Jul 09 23:00:00 1997 From: randy97 Subject: http://www.love.com Newsgroups: bionet.molbio.proteins.7tms_r NNTP-Posting-Host: pgh.nauticom.net Message-ID: <33c528d1.1@pgh.nauticom.net> Date: 10 Jul 97 18:24:17 GMT Lines: 9 Path: biosci!agate!hammer.uoregon.edu!hunter.premier.net!europa.clark.net!newsfeed.internetmci.com!pgh.nauticom.net!pgh.nauticom.net Looking to find people in your area that enjoy the same things as this newsgroup? Check out http://www.love.com It's free, it's new, and it's awesome. Rand From owner-7tms_r@net.bio.net Thu Jul 10 23:00:00 1997 Path: biosci!daresbury!bioftp.unibas.ch!news.vub.ac.be!news.belnet.be!news-zh.switch.ch!news-ge.switch.ch!news.grnet.gr!btnet-feed3!unlisys!fu-berlin.de!newsfeed.nacamar.de!news-peer.sprintlink.net!news-pull.sprintlink.net!news-in-east.sprintlink.net!news.sprintlink.net!Sprint!202.243.48.20!news.gol.com!not-for-mail From: "Paul Sully" Newsgroups: bionet.molbio.proteins.7tms_r Subject: Japanese Professor Date: 11 Jul 1997 23:47:47 GMT Organization: Paul Sully International Academy Lines: 11 Message-ID: <01bc8e0b$eafa7c00$8371d8cb@thinkpad> NNTP-Posting-Host: tc-1-123.osaka.gol.com X-Newsreader: Microsoft Internet News 4.70.1157 I'm posting this message for a friend of mine. He is a Japanese Professor at Osaka University of Health and sports Sciences. He wishes to do a sabbatical year, starting April 1st 1998, working on the effects of walking on the lipoprotein metabolism of vascular endothelium in dietary rats. He would like to study molecular biology and wishes to obtain a place in a laboratory, in Australia, at Japan's expense. If anybody could supply any information about Universities in Australia dealing in these areas or is able to make any suggestions, then I would be most grateful. Please reply directly to sully@gol.com. Thank you. From owner-7tms_r@net.bio.net Thu Jul 10 23:00:00 1997 Path: biosci!OBZOR.BIO21.ACAD.BG!ttsonev From: ttsonev@OBZOR.BIO21.ACAD.BG (biofac) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Help, please! Date: 11 Jul 1997 08:32:02 -0700 Organization: Institute of Plant Physiology Lines: 89 Sender: daemon@net.bio.net Distribution: world Message-ID: <33C651C2.23EC24C4@obzor.bio21.acad.bg> NNTP-Posting-Host: net.bio.net Sofia University "St Kliment Ohridsky" Faculty of Biology 8, Dragan Tsankov Blvd 1421 Sofia, Bulgaria Tel: (3592) 63301 Fax (3592) 658079 Dear Sirs, The daughter of a colleague of ours, chief researcher in the "Cytology, Histology and Embriology" at the Biological Faculty of the Sofia University "St Kliment Ohridsky" is suffering from an acute form of a myelloblast leukaemia. The personnel of the Department and the parents of the girl address you with the request for financial funds, necessary for bone marrow transplant to be conducted abroad. The required sum amounts to USD 80 000 - 1000 000. We also appeal to: Bulgarian and foreign banks, foundations, charity organisations, private and state companies, the academic community and private persons to assist, and we hope that they will be charitable and will respond to our deeply human call for help. The donation account in the name of Desislava Plamenova Georgieva was opened in Biochim Ad, Sredetz Branch, 1 Ivan Vazov Street, Sofia: BGL 660084225 No. 1003363608 USD 660084225 No. 18323000103363601 DM 660084225 No. 18323010103363608 We rely on your charity and on your parental understanding for the destiny of the 18-year old girl who probably will be given the chance to live thanks to your assistance. MEMBERS OF THE DEPARTMENT COMMERCIAL BANK "BIOCHIM" SWIFT CODE: CBBIBGSF BRANCH "SREDETZ": 1040, SOFIA, 1, IVAN VAZOV STR. NOSTRO ACCOUNTS ABROAD: (as at 10.10.1996): BANK currency ACCOUNT SWIFT AMERICAN EXPRESS USD 300700823 AEIBUS33 BANK, New York BANKERS TRUST CO., USD 04090904 BKTRUS33 NEW YORK BANK OF NEW YORK USD 8900058935 IRVTUS3N NEW YORK CITIBANK N.A., USD 36017613 CITIUS33 NEW YORK CREDIT LYONNAIS, USD 01-19782-0001-00 CRLYUS33 NEW YORK DEUTSCHE BANK AG, USD 923 3610 00 DEUTDEFF FRANKFURT AM MAIN LLOYDS BANK PLC, USD 12308436 LOYDGB2L LONDON NATIONAL BANK OF USD 040/504097 ETHNGRAA GREECE, ATHENS STOPANSKA BANKA DD, USD 100.840.2 STOBMK2X SKOPJE SWISS BANK CORP., USD 101-WA-343749-000 SBCOUS33 NEW YORK AMERICAN EXPRESS BANK DEM 424-007406906 AEIBDEFX FRANKFURT AM MAIN BANKERS TRUST CO., DEM 810150027 BKTDDEFF FRANKFURT AM MAIN BAYERISCHE HYPOTEKEN DEM 6450026539000 HYPODEMM UND WECHSEL BANK MUNICH BAYERISCHE VEREINSBANK, DEM 68100470 BVBEDEMM MUNICH BfG BANK AG, DEM 100 34 777 00 BFGFDEFF FRANKFURT AM MAIN COMMERZBANK AG, DEM 400871916300 COBADEFF FRANKFURT / MAIN DEUTSCHE BANK AG, DEM 100 9233610 0000 DEUTDEFF FRANKFURT AM MAIN DRESDNER BANK AG, DEM 499/08021429/00/000 DRESDEFF FRANKFURT AM MAIN DG BANK AG, DEM 11/1000/1632 DGBKDEFF FRANKFURT AM MAIN STOPANSKA BANKA DD, DEM 100.280.3 STOBMK2X SKOPJE WESTDEUTSCHE LANDESBANK DEM 55 891 WELADEDD DUESSELDORF Correspondent Banking: Phone +359 2/980 4926, Fax +359 2/881147 Telex: 23921, 24757 From owner-7tms_r@net.bio.net Thu Jul 10 23:00:00 1997 Path: biosci!agate!howland.erols.net!newsfeed.nacamar.de!fu-berlin.de!schupha8.pharmazie.fu-berlin.DE!not-for-mail From: Holger STARK Newsgroups: bionet.molbio.proteins.7tms_r Subject: atomic resolution rhodopsin TM core model Date: Fri, 11 Jul 1997 09:28:39 +0200 Organization: Freie Universitaet Berlin, Inst. f. Pharmazie I, Germany Lines: 98 Message-ID: <33C5E0A5.2AD01700@schunet1.pharmazie.fu-berlin.de> NNTP-Posting-Host: schupha8.pharmazie.fu-berlin.de (160.45.56.107) Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 X-Access: 16 17 19 X-Mailer: Mozilla 4.0b5 [en] (Win95; I) X-Priority: 3 (Normal) Zur Information und evtl. allg. Nutzen: Atomic resolution models of the transmembrane domain of bovine rhodopsin and metarhodopsin II, which we (I.Pogozheva, A. Lomize, H. Mosberg) recently published in Biophysical J., v. 70, pp. 1963-1985, May 1997, have been deposited in the Protein Data Bank as files 1bok and 1boj. These models were to be released by July 1, but unfortunately, the files are still under "REVIEW" status at PDB. Anyone interested in receiving these coordinates in advance can do so by sending me a request via e-mail. The structure of the transmembrane 7-helix-bundle was calculated using an iterative distance geometry refinement with an evolving system of hydrogen bonds, formed by intramembrane polar side-chains in various proteins from the GPCR family and collectively applied as distance constraints. The "average" 7-helix-bundle structure thus obtained provides H-bonding of nearly all buried polar side-chains simultaneously in the 410 GPCRs considered. The "average" atomic model of the alpha-bundle has been tested by using it as a template to calculate the transmembrane domains of various GPCRs in addition to the rhodopsin/MetaII examples discussed in the Biophys.J. article. Each resulting GPCR model contains a binding pocket complementary in shape and polarity to its natural and synthetic ligands and is consistent with experimental data. At present, we have calculated and compared with experimental data the transmembrane domains of 28 different GPCRs, including red cone opsin, invertebrate (crayfish) rhodopsin, as well as opioid, chemokine, melatonin, purine, glycoprotein, and cationic amine receptors (for some of these, the results are still preliminary) and their complexes with many (mostly, relatively rigid) ligands The calculations of the individual receptor structures from the same "average" model are time-consuming, since they include determination of conformers for many side-chains which replace those found in the "average" model, some rearrangements of H-bond networks, small shifts of helices, appearance of Pro kinks, and so on. This work can not be done by using standard "modeling by homology" procedures; but requires distance geometry refinement with evolving constraints for each specific GPCR modeled. We have also developed a computational approach for modeling loops connecting the transmembrane helices, and have obtained preliminary results for the extracellular loops of opioid receptors and red opsin. We are interested in further experimental testing of the model. The most obvious test would be a direct fit with 6-7 A resolution electron microscopy 3D maps of frog rhodopsin, but unfortunately, the maps are unavailable at this time. The model can also be tested by incorporating disulfide bonds between spatially close cysteines, for example from Table 2 in our article in Biophysical Journal. Henry I. Mosberg e-mail: him@umich.edu Professor of Medicinal Chemistry Phone: (313)764-8117 College of Pharmacy FAX: (313)763-5633 University of Michigan Ann Arbor, MI 48109-1065 GRUSS HOLGER ____________________________________________________ Dr. Holger STARK Freie Universitaet Berlin Institut fuer Pharmazie I Koenigin-Luise-Str. 2+4 D-14195 Berlin, GERMANY Tel.: +49 - 30 - 838 3295/3928 Fax: +49 - 30 - 838 3854 E-mail: stark@schunet1.pharmazie.fu-berlin.de Home page: http://www.pharmazie.fu-berlin.de _____________________________________________________ From owner-7tms_r@net.bio.net Fri Jul 11 23:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.proteins.7tms_r Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 12 Jul 1997 02:00:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199707120900.CAA15243@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-7tms_r@net.bio.net Sat Jul 12 23:00:00 1997 Path: biosci!rutgers!gatech!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!europa.clark.net!dispatch.news.demon.net!demon!bullseye.news.demon.net!demon!newsgate.unisource.nl!halley.pi.net!news From: jacob Newsgroups: bionet.molbio.proteins.7tms_r Subject: Asian Ladies Date: 13 Jul 1997 18:53:56 GMT Organization: World Access/Planet Internet Message-ID: <5qb884$rtk@halley.pi.net> NNTP-Posting-Host: 145.220.210.27 Lines: 10 I've found the pefect site with nude Asian ladies. Much more than you can find in any newsgroup. http://home.pi.net/~sappie/playboy.htm From owner-7tms_r@net.bio.net Mon Jul 14 23:00:00 1997 Path: biosci!sbphrd.com!Jeffrey_M_Stadel From: Jeffrey_M_Stadel@sbphrd.com (Jeffrey M Stadel) Newsgroups: bionet.molbio.proteins.7tms_r Subject: 7tmr knockouts Date: 15 Jul 1997 12:04:56 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: <9707152205.AA4019@pho903.sbphrd.com> NNTP-Posting-Host: net.bio.net Does anyone have a bibliography of 7TM receptor knockouts or could point me in the right direction to obtain that information? Thanks for the help, Jeff Stadel From owner-7tms_r@net.bio.net Tue Jul 15 23:00:00 1997 Path: biosci!agate!hammer.uoregon.edu!vixen.cso.uiuc.edu!howland.erols.net!gatech!news-relay.ncren.net!ussun2n!news@ussun2n.glaxo.com From: TAT32111@Glaxo.com (Timothy True) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Dexmedetomidine Date: Wed, 16 Jul 1997 23:53:04 GMT Organization: Glaxo Wellcome plc Lines: 7 Message-ID: <33cd5ce1.8260170@netnews.glaxo.com> NNTP-Posting-Host: 152.51.4.59 X-Newsreader: Forte Free Agent 1.11/16.235 Could someone be so kind as to provide me with a commercial source for the alpha2 agonist Dexmedetomidine. It would be much appreciated. Thanks, Timothy True From owner-7tms_r@net.bio.net Wed Jul 16 23:00:00 1997 Path: biosci!HOTMAIL.COM!qwart From: qwart@HOTMAIL.COM (JB) Newsgroups: bionet.molbio.proteins.7tms_r Subject: HB-EGF as a protooncogene??? Date: 17 Jul 1997 11:01:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: <33CE5B9F.BB3@hotmail.com> NNTP-Posting-Host: net.bio.net What=92s your opinion? Recently we have discussed in our lab if growth = factors like HB-EGF can be considered a = protooncogenes. To my opinion: = when considering the definition of a protooncogene is a gene = involved in proliferation and differentiation pathways, and when = mutated it causes in concert with other functionally related genes = a cancer, then HB-EGF could be also considered a protooncogene. From owner-7tms_r@net.bio.net Wed Jul 23 23:00:00 1997 Path: biosci!essex.ac.uk!goulpj From: goulpj@essex.ac.uk (Paul Gouldson) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 24 Jul 1997 07:26:04 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net subscribe 7tms_r From owner-7tms_r@net.bio.net Wed Jul 23 23:00:00 1997 Path: biosci!essex.ac.uk!goulpj From: goulpj@essex.ac.uk (Paul Gouldson) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Post Doc wanted Date: 24 Jul 1997 10:06:48 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear All, In the very near future I will be finishing my PhD (with Dr Chris Reynolds) which has involved modelling GPCRs. The work has revolved around a possible activation mechanism which includes agonist induced receptor dimerisation, and novel methods for modelling this class of receptor. I have used various techniques (molecular dynamics, correlated mutation analysis, molecular graphics)during my research and have a good knowledge of UNIX. Currently I have 5 publications with two more submitted. I would like to continue in the GPCR field but along a more molecular biology route, but still have dealings with molecular modelling if possible. I am therefore looking for a lab that can offer good molecular biology training, but might lack or require the help of a proficient modeller. If anyone has any openings (or knows of any openings) or would like a copy of my CV please e-mail me or Snail Mail me (see below). Many thanks for your time Paul Gouldson _______________________________________________________________________ Hockey players do it with a E-Mail Address nice swing (as do golfers).... goulpj@essex.ac.uk _ \\ Snail Mail \\ Paul Gouldson, \\ Postgradute Pigeon Holes, \\ Dept. Biological Sciences (Central Campus) \\ University of Essex, \\ Colchester, \\,, Essex CO4 3SQ. `-' England _______________________________________________________________________ From owner-7tms_r@net.bio.net Thu Jul 24 23:00:00 1997 Path: biosci!daresbury!not-for-mail From: Newsgroups: bionet.molbio.proteins.7tms_r Subject: G protein expression -HEK Date: 25 Jul 1997 15:30:19 +0100 Lines: 23 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5rad9r$ke2@mserv1.dl.ac.uk> Original-To: 7tms_r@dl.ac.uk > Can anyone please tell me which G-proteins are expressed in HEK 293 >cells? (in particular, are proteins of the G12 family expressed?) Thanks, Lena. Lena Brydon, Department of Immuno-Pharmacology, I.C.G.M.,C.N.R.S.,22 rue Mechain, Paris. brydon@icgm.cochin.inserm.fr From owner-7tms_r@net.bio.net Mon Jul 28 23:00:00 1997 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.direct.ca!news-sea-19.sprintlink.net!news-in-west.sprintlink.net!news.sprintlink.net!Sprint!130.207.244.18!gatech!huron.eel.ufl.edu!usenet.nerdc.ufl.edu!usenet.ufl.edu!receptor.pharmacology.ufl.edu!user From: jharrison@qm.server.ufl.edu (Jeffrey K. Harrison) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Postdoctoral position Followup-To: bionet.molbio.proteins.7tms_r Date: Tue, 29 Jul 1997 16:14:02 -0500 Organization: University of Florida Lines: 8 Message-ID: NNTP-Posting-Host: receptor.pharmacology.ufl.edu POSTDOCTORAL POSITION to study the expression, function, and regulation of chemokine receptors in the central nervous system. A background in molecular biology, immunohistochemistry, and neuroscience is desirable. Send curriculum vitae and the names of three references to: Jeffrey K. Harrison, Ph.D., Department of Pharmacology and Therapeutics, Box 100267, University of Florida, Gainesville, FL 32610-0267. E-mail: harrison@pharm.med.ufl.edu. The University of Florida is an Affirmative Action/Equal Opportunity Employer. From owner-7tms_r@net.bio.net Tue Jul 29 23:00:00 1997 Path: biosci!tokyo-u-fish.ac.jp!hushio From: hushio@tokyo-u-fish.ac.jp (Hideki Ushio) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: transducin antibodies Date: 30 Jul 1997 18:29:17 -0700 Organization: Tokyo University of Fisheries Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <33E06ADE.55A7@tokyo-u-fish.ac.jp> Reply-To: hushio@tokyo-u-fish.ac.jp NNTP-Posting-Host: net.bio.net Dear Doctors: I am now seeking for any types of antibodies against rod transducins or the related peptides. If someone knows what companies deal in them, please let me know. Best wishes, Hideki Ushio Hideki Ushio, Ph.D. Associate Professor Tokyo University of Fisheries Tokyo 108, Japan Phone: +81 3 5463 0615 FAX: +81 3 5463 0627 e-mail: hushio@tokyo-u-fish.ac.jp From owner-7tms_r@net.bio.net Wed Jul 30 23:00:00 1997 Path: biosci!daresbury!not-for-mail From: yc3 Newsgroups: bionet.molbio.proteins.7tms_r Subject: Gi/Go in Sf21/Sf9 Date: 31 Jul 1997 14:15:09 +0100 Lines: 8 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5rq34t$maq@mserv1.dl.ac.uk> Original-To: 7tms_r@dl.ac.uk Does anybody know what G-proteins are endogenous to Sf21 or Sf9 insect cells? I am particularly interested in Gi/Go G-proteins and have been confused by the apparent inconsistancy in the literature. Many thanks, Yolande Cordeaux From owner-7tms_r@net.bio.net Wed Jul 30 23:00:00 1997 Path: biosci!bms.com!watson_j From: watson_j@bms.com (John Watson) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: transducin antibodies Date: 31 Jul 1997 06:49:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 29 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net >Dear Doctors: > >I am now seeking for any types of antibodies against rod transducins or >the related peptides. If someone knows what companies deal in them, >please let me know. Santa Cruz Biotechnology (http://www.scbt.com) sells anti-rod (G-alpha-t1) and anti-cone (G-alpha-t2) transducin polyclonal sera. You should, however, take their performance claims with a a fair degree of skepticism (IMO). AJW ------------------------------------------------------------------------ __ __ __ A. John Watson, Ph.D. /__/__/__/\ Bristol-Myers Squibb Company /__/__/__/\/\ Pharmaceutical Reseach Institute /__/__/__/\/\/\ Nervous Systems Molecular Biology, Dept. 405 \__\__\__\/\/\/ 5 Research Parkway \__\__\__\/\/ Wallingford, CT 06492 \__\__\__\/ watson_j@bms.com | 203.284.6745 voice | "If you're not part of the solution, 203.284.7569 fax | you're part of the precipitate." ------------------------------------------------------------------------ From owner-7tms_r@net.bio.net Thu Jul 31 23:00:00 1997 Path: biosci!BIOCSERVER.BIOC.CWRU.EDU!roth From: roth@BIOCSERVER.BIOC.CWRU.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: Post-doctoral positions available Date: 1 Aug 1997 07:26:38 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 37 Sender: daemon@net.bio.net Distribution: world Message-ID: <199708011427.OAA00624@biocserver.BIOC.CWRU.Edu> NNTP-Posting-Host: net.bio.net > >Two NIH-funded positions are currently available in the laboratory of Bryan Roth in the Department of Biochemistry at Case Western Reserve University Medical School. Currently my lab is studying structure-function aspects of serotonin receptors with a particular emphasis on 5-HT2A receptors. These receptors are essential for mediating the actions of atypical antipsychotic drugs, antidepressants and hallucinogens. For a complete description of work ongoing, please look at my web page which is given below. > >One position requires expertise in general molecular biology techniques while the other requires expertisse in cell biology and protein purification. Salary is negotiable. > >Both positions are firm for up to 4 years, although post-doctoral fellows in my lab typically obtain real jobs (e.g. industrial or academic) after 2-3 years in the lab. > >If intersted, please fax or e-mail your cv and three letters of recommendation to the address below: > ============================================================= Bryan L.Roth MD, PhD Associate Professor Departments of Psychiatry, Biochemistry and Neurosciences Room W438 Case Western Reserve University Medical School 10900 Euclid Avenue Cleveland, OH 44106-4935 216-368-4544 (Fax) 216-368-2730 (Office) roth@biocserver.cwru.edu web page: http://www.cwru.edu/CWRU/Dept/Med/biochemistry/faculty/roth.html