From owner-7tms_r@net.bio.net Tue Aug 05 23:00:00 1997 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!howland.erols.net!csir.uni.net.za!wits.uni.net.za!nntp.wits.ac.za!pc85.med.wits.ac.za!126umesh From: 126umesh@chiron.wits.ac.za (Bodalina, Umesh) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Help with Western Blot technique Date: Tue, 5 Aug 1997 09:01:53 GMT Organization: Univerity of the witwatersrand Lines: 10 Message-ID: <126umesh.178@chiron.wits.ac.za> NNTP-Posting-Host: pc85.med.wits.ac.za Help Please I am using Western blot techniques to detect my proteins. I do get bands for my proteins but they are too faint to be scanned. I use the DAB-CN method for staining the proteins. I've also tried chemiluminescence staining method, also with very little success. Has anybody got any suggestions that I could try out inorder to get darker bands. Please EMail me at: 126umesh@chiron.wits.ac.za THANX Umesh From owner-7tms_r@net.bio.net Thu Aug 07 23:00:00 1997 Path: biosci!PO.CWRU.EDU!pre From: pre@PO.CWRU.EDU ("Paul Ernsberger, Ph.D.") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Bygone methods: NH4SO4 Date: 8 Aug 1997 11:22:05 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <199708081822.OAA10153@cornelius.INS.CWRU.Edu> NNTP-Posting-Host: net.bio.net Could anyone tell me or venture a guess what concentration of ammonium sulfate would be needed to precipitate PKC? (mixed isozymes --if it matters-- from PC12 cell lysate) I need a quick way to remove detergent without dialysis. Paul Ernsberger, Ph.D., Associate Professor of Medicine, Pharmacology and Neuroscience Case Western Reserve School of Medicine, 10900 Euclid Ave., Cleveland, OH 44106-4982 Email: pre@po.cwru.edu FAX: (216) 368-4752 Visit the Imidazoline Receptor Web page at http://www/mmcc.monash.edu.au/phimr/ireceptor From owner-7tms_r@net.bio.net Thu Aug 07 23:00:00 1997 Path: biosci!PO.CWRU.EDU!pre From: pre@PO.CWRU.EDU ("Paul Ernsberger, Ph.D.") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Bygone methods: NH4SO4 Date: 8 Aug 1997 11:21:10 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <199708081821.OAA10042@cornelius.INS.CWRU.Edu> NNTP-Posting-Host: net.bio.net Could anyone tell me or venture a guess what concentration of ammonium sulfate would be needed to precipitate PKC? (mixed isozymes --if it matters-- from PC12 cell lysate) I need a quick way to remove detergent with dialysis. Paul Ernsberger, Ph.D., Associate Professor of Medicine, Pharmacology and Neuroscience Case Western Reserve School of Medicine, 10900 Euclid Ave., Cleveland, OH 44106-4982 Email: pre@po.cwru.edu FAX: (216) 368-4752 Visit the Imidazoline Receptor Web page at http://www/mmcc.monash.edu.au/phimr/ireceptor From owner-7tms_r@net.bio.net Thu Aug 07 23:00:00 1997 Path: biosci!DOMAIN.COM!defaultuser From: defaultuser@DOMAIN.COM (defaultuser) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Expression of a G-Protein coupled receptor Date: 8 Aug 1997 11:18:05 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 6 Sender: daemon@net.bio.net Distribution: world Message-ID: <33EB62F8.2B6F0DD9@domain.com> NNTP-Posting-Host: net.bio.net Who has experience in the field of receptor-expression in eucaryotic cells and can help me choosing a effective expression-system? The aim of my work is to overproduce a G-protein coupled receptor for purification purposes. From owner-7tms_r@net.bio.net Mon Aug 11 23:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.proteins.7tms_r Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 12 Aug 1997 02:00:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199708120900.CAA08234@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-7tms_r@net.bio.net Mon Aug 11 23:00:00 1997 Path: biosci!daresbury!not-for-mail From: Paul Gouldson Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe 7TMS_R Date: 12 Aug 1997 15:09:48 +0100 Lines: 1 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5spqrc$glb@mserv1.dl.ac.uk> X-Sender: goulpj@serultra0 Original-To: 7tms_r@dl.ac.uk subscribe 7TMS_R From owner-7tms_r@net.bio.net Mon Aug 11 23:00:00 1997 Path: biosci!agate!spool.mu.edu!uwm.edu!vixen.cso.uiuc.edu!news-peer.sprintlink.net!news.sprintlink.net!Sprint!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!infeed2.internetmci.com!newsfeed.internetmci.com!news-feed1.eu.concert.net!unlisys!cs.tu-berlin.de!uni-erlangen.de!winx03!wpxx02!not-for-mail From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Bygone methods: NH4SO4 Date: Tue, 12 Aug 1997 19:00:04 +0200 Organization: University of Wuerzburg, Germany Lines: 17 Distribution: world Message-ID: References: <199708081822.OAA10153@cornelius.INS.CWRU.Edu> NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Paul Ernsberger, Ph.D. (pre@PO.CWRU.EDU) wrote: > Could anyone tell me or venture a guess what concentration of ammonium > sulfate would be needed to precipitate PKC? > (mixed isozymes --if it matters-- from PC12 cell lysate) > I need a quick way to remove detergent without dialysis. You might want to try extractigel columns, available from Pierce. (As to the necessary ammonium sulphate concentration -- I have no idea. Many proteins precipitate at around 60% (w/w).) --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP3 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-7tms_r@net.bio.net Fri Aug 15 23:00:00 1997 Path: biosci!agate!hammer.uoregon.edu!vixen.cso.uiuc.edu!howland.erols.net!infeed1.internetmci.com!newsfeed.internetmci.com!152.163.199.19!portc03.blue.aol.com!newstf02.news.aol.com!audrey02.news.aol.com!not-for-mail From: huntpharm@aol.com (Huntpharm) Newsgroups: bionet.molbio.proteins.7tms_r Subject: US-NC-HEAD OF STRUCTUAL CHEMISTRY Date: 16 Aug 1997 19:49:03 GMT Lines: 13 Message-ID: <19970816194900.PAA17962@ladder02.news.aol.com> NNTP-Posting-Host: ladder02.news.aol.com X-Admin: news@aol.com Organization: AOL http://www.aol.com I am looking for a PhD Scientist to be the Head of the Department of Structural Chemistry. The candidate will have a strong background in either X-Ray Crystallography or Computational Chemistry . As the person in this visionary role, you will be responsible for leading a group of sixteen scientists and growing. You should possess a PhD with 5+ years experience and strong publication record. We are a leading pharmaceutical company with research facilities in North Carolina and can provide excellent benefits (health insurance, dental, and vision plan, bonus program, paid vactation and more). Excellent compensation package. A high impact, high profile position with excellent opportunity for advancement. Please contact Scott Shanes by phone at 609-584-8733 Ext. 218, fax CV and cover letter to 609-584-9575 or E-Mail to sis@diedremoire.com From owner-7tms_r@net.bio.net Tue Aug 19 23:00:00 1997 Path: biosci!RUBENS.ITS.UNIMELB.EDU.AU!pms From: pms@RUBENS.ITS.UNIMELB.EDU.AU Newsgroups: bionet.molbio.proteins.7tms_r Subject: receptor genes Date: 20 Aug 1997 04:14:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: <3.0.2.32.19970820211100.00a06120@rubens.its.unimelb.edu.au> NNTP-Posting-Host: net.bio.net Can anyone tell me if there is evidence for transcriptional regulatory elements in G-protein receptor genes occuring more than ~4kb upstream of exon 1 and/or regulatory elements in the 3' end of the gene ? Many thanks to anyone who can help. Patrick ---------------------------------------------------------------------------- ------------- Patrick M. Sexton, PhD St. Vincent's Institute of Medical Research 41 Victoria Parade, Fitzroy 3065, Victoria, AUSTRALIA Phone: 61-3-9288-2480 Fax: 61-3-9416-2676 E-mail: pms@rubens.its.unimelb.edu.au From owner-7tms_r@net.bio.net Tue Aug 19 23:00:00 1997 From: aslkfjds093e@djfasdlfj.com Subject: Pamela & Tommy Lee's Secret Sex Tape Newsgroups: bionet.molbio.proteins.7tms_r NNTP-Posting-Host: ppp10225.la.inreach.net Message-ID: <33fb6090.0@news.inreach.com> Date: 20 Aug 97 21:24:32 GMT Lines: 21 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!infeed1.internetmci.com!newsfeed.internetmci.com!199.60.229.3!newsfeed.direct.ca!news-sea-19.sprintlink.net!news-in-west.sprintlink.net!news.sprintlink.net!Sprint!208.25.48.5!news.inreach.com!ppp10225.la.inreach.net DON'T BE FOOLED BY IMITATIONS There is only one Pam & Tommy Sex Tape and it is available here for a limited time only. Others may claim to have it for sale but this is the only Hard Core Copyrighten version in existence. See them bare their soles and bodies in public places, but observe closely, a very private blend of love and sex that they thought the public would never see. Visit: http://www.pamsex.com http://www.pamsex.com From owner-7tms_r@net.bio.net Wed Aug 20 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!news-spur1.maxwell.syr.edu!news.maxwell.syr.edu!newsfeed.nacamar.de!fu-berlin.de!news.th-darmstadt.de!uni-erlangen.de!winx03!wpxx02!not-for-mail From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: type I vs. type II membrane proteins Date: Thu, 21 Aug 1997 16:31:41 +0200 Organization: University of Wuerzburg, Germany Lines: 16 Message-ID: References: NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] klennon@acs.bu.edu wrote: > I was just wondering what the criteria are for determining whether a membrane > protein is type I or type II. The classical definition is that a type I membrane protein has its N-terminus extracellular and its C-terminus in the cytoplasm, while a type II membrane protein has the vice-versa orientation. Both type I and II proteins have a single transmembrane domain. Type III proteins have multiple transmembrane domains. --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP3 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-7tms_r@net.bio.net Wed Aug 20 23:00:00 1997 Path: biosci!prius.jnj.com!MMilik From: MMilik@prius.jnj.com ("Milik, Mariusz [PRI]") Newsgroups: bionet.molbio.proteins.7tms_r Subject: RE: type I vs. type II membrane proteins Date: 21 Aug 1997 09:58:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 48 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net >---------- >From: klennon@ACS1.BU.EDU[SMTP:klennon@ACS1.BU.EDU] >Sent: Thursday, August 21, 1997 5:31 AM >To: 7tms_r@net.bio.net >Subject: type I vs. type II membrane proteins > >Hi all, > >I was just wondering what the criteria are for determining whether a membrane >protein is type I or type II. I have seen that designation in a million >papers, but upon consulting two biochem books (Stryer and Garrett & Grisham), >a book on Protein Structure & Function, and three biochem PhDs, I was left >un-enlightened. I'm assuming it has something to do with either the number of >transmembrane domains, but I'm not sure. > >Any help with this (even if it's a reference so I can learn instead of being >spoonfed knowledge!!), would be greatly appreciated! > >Thanks. >Kelley >BU Med Center > Hi Kelly, The best classification paper for membrane proteins is (IMHO) "The Structure and Insertion of Integral Proteins in Membranes" by S.J. Singer (Ann.Rev.Cell.Biol. 1990, 6:247-96). Simple schematic view of this classification you can find in TIBS 21 (May 1996) , article: "Type VI membrane proteins?" by S.Howell and P.Crine. Type I and II membrane proteins have one TM domain but different orientation. Type I - N-terminus-outside, type II - N-terminus-inside. Type III - multiple TM with different topologies (GPCR's are III ), type IV are channels made up of multimers of subunits and type V are attached to the membrane by covalently bound lipids (no "classical" TM domains). Best Mariusz >Mariusz Milik, Ph.D. >Bioinformatics >The R.W.Johnson Pharmaceutical Res. Inst. >San Diego, CA > > > From owner-7tms_r@net.bio.net Wed Aug 20 23:00:00 1997 Path: biosci!agate!newsfeed.kornet.nm.kr!howland.erols.net!newsxfer3.itd.umich.edu!news.eecs.umich.edu!news.bu.edu!MED-ORALBIO7.bu.edu!klennon From: klennon@acs.bu.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: type I vs. type II membrane proteins Date: Thu, 21 Aug 1997 12:31:18 GMT Organization: Boston University Lines: 15 Message-ID: NNTP-Posting-Host: med-oralbio7.bu.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Hi all, I was just wondering what the criteria are for determining whether a membrane protein is type I or type II. I have seen that designation in a million papers, but upon consulting two biochem books (Stryer and Garrett & Grisham), a book on Protein Structure & Function, and three biochem PhDs, I was left un-enlightened. I'm assuming it has something to do with either the number of transmembrane domains, but I'm not sure. Any help with this (even if it's a reference so I can learn instead of being spoonfed knowledge!!), would be greatly appreciated! Thanks. Kelley BU Med Center From owner-7tms_r@net.bio.net Wed Aug 20 23:00:00 1997 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!infeed1.internetmci.com!newsfeed.internetmci.com!204.238.120.130!jump.net!grunt.dejanews.com!not-for-mail Date: Thu, 21 Aug 1997 10:36:23 -0600 From: VEIUSA@aol.com Subject: Molecular Biology Question Newsgroups: bionet.molbio.proteins.7tms_r Message-ID: <872177130.6237@dejanews.com> Organization: Deja News Posting Service X-Article-Creation-Date: Thu Aug 21 15:25:30 1997 GMT X-Originating-IP-Addr: 152.170.70.24 (170-70-24.ipt.aol.com) X-Http-User-Agent: Mozilla/1.22 (compatible; MSIE 2.0; Windows 95) X-Authenticated-Sender: VEIUSA@aol.com Lines: 12 I have been asked to attend cell biology meeting in 12/97. THE PROBLEM, I have no idea of how my company's products are used in this scientific discipline. Are stereo microscopes used at all by molecular biologists?? My guess is compound microscopes are used more extensively, is this a correct assessment?? If stereo is used, what magnifications or special lighting or options is req'd? What % of time is spent w/ scopes?? Stereo vs compound if applicable. Any insights offered will be greatly appreciated to improve my awareness. Thx in advance to all responding experts. Sincerrely, Mark R.:) -------------------==== Posted via Deja News ====----------------------- http://www.dejanews.com/ Search, Read, Post to Usenet From owner-7tms_r@net.bio.net Thu Aug 21 23:00:00 1997 Path: biosci!bloom-beacon.mit.edu!panix!news.eecs.umich.edu!newsxfer3.itd.umich.edu!portc01.blue.aol.com!audrey02.news.aol.com!not-for-mail From: forgetu2@aol.com (Forgetu2) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Molecular Biology Question Date: 22 Aug 1997 12:01:02 GMT Lines: 7 Message-ID: <19970822120100.IAA17415@ladder02.news.aol.com> NNTP-Posting-Host: ladder02.news.aol.com X-Admin: news@aol.com Organization: AOL http://www.aol.com References: <872177130.6237@dejanews.com> I *think* they use microscopes in FISH (Fluorescent in situ hybridization) and looking at the fluorescence of cells in general. Hope this helps some, Jon From owner-7tms_r@net.bio.net Thu Aug 21 23:00:00 1997 Path: biosci!pt.Cyanamid.COM!ramaswam From: ramaswam@pt.Cyanamid.COM (Ramaswamy Nilakantam) Newsgroups: bionet.molbio.proteins.7tms_r Subject: gpcr ligands Date: 22 Aug 1997 07:40:43 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <199708221439.AA270410799@primail.pr.cyanamid.com> NNTP-Posting-Host: net.bio.net Hi, Can someone give me some examples of 7TM ligands (small organic molecules) that bind: A. in the interior of the helical bundle B. in the extracellular loops Thanks Ramaswamy Nilakantan Wyeth-Ayerst Research From owner-7tms_r@net.bio.net Sun Aug 24 23:00:00 1997 Path: biosci!FERRE.SACLAY.CEA.FR!nicolas From: nicolas@FERRE.SACLAY.CEA.FR (Nicolas Ancellin) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Looking for a postdoctoral position Date: 25 Aug 1997 07:58:41 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 37 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Looking for a postdoctoral position. I am writing for the possibility of doing postdoctoral research work in one laboratory. My research work is mainly focus on the acute desensitization of the G-protein coupled receptors ( and particularly the phospholipase-Cb linked receptors). I hope to get a postdoctoral position in the related research areas (receptors, cell biology...). Currently, I am in the process of doing my Ph.D. (thesis) in France, and I am scheduled to finish my Ph.D. program in February of 1997. Please feel free to contact me at nicolas@ferre.saclay.cea.fr if you require any further information.(CV, references...) Sincerely yours Keywords : Scientific : Intracellular Signaling / Receptors / G Proteins / Phospholipase C / Adenylyl cyclase / Kinases / Desensitization / Internalization / Pharmacological uncoupling / Phosphorylation / Cross-Talk / Orphan receptors Technical : Heterologous expression ( Xenopus oocytes, HEK293, CHO, COS7 ) / Calcium measurement ( luminescence-Aequorin, fluorescence-FURA2 ) / cAMP Assay (EIA) / Binding / Western Blot / Immunoprecipitation / Immunofluorescence / Recombinant DNA ( cloning...) / Directed mutagenesis / Chimera / RT-PCR / Fusion Protein ------------------------------------------------------------------------------- Nicolas Ancellin Departement de Biologie Cellulaire et Moleculaire Service de Biologie Cellulaire Bat 520 p2, CEA CEN-Saclay F91191 Gif sur Yvette Cedex, France Tel (33) 01 69 08 36 27 fax (33) 01 69 08 35 70 nicolas@ferre.saclay.cea.fr -------------------------------------------------------------------------------- From owner-7tms_r@net.bio.net Mon Aug 25 23:00:00 1997 Path: biosci!agate!newsfeed.kornet.nm.kr!howland.erols.net!infeed1.internetmci.com!newsfeed.internetmci.com!198.82.160.249!solaris.cc.vt.edu!hp.fciencias.unam.mx!not-for-mail From: Adriana Bañuelos Pánuco Newsgroups: bionet.molbio.proteins.7tms_r Subject: cells contaminatio Date: Tue, 26 Aug 1997 17:48:00 -0400 Organization: A poorly-installed InterNetNews site Lines: 7 Message-ID: <34034F10.3E84@lambda.gene.cinvestav.mx> NNTP-Posting-Host: ribozyme.gene.cinvestav.mx Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (X11; I; SunOS 5.5 sun4m) I`m a doctorate student. I`m going to work with novel cervical cancercells lines. I want to study the state of some cellular genes, but there is a strong suspicion that this lines cells are contaminated with HeLa cells. How can I probe this possible contamination? I don`t know any speccific characteristic of HeLa taht could helps me. Adriana Banuelos. From owner-7tms_r@net.bio.net Mon Aug 25 23:00:00 1997 Path: biosci!daresbury!uninett.no!news-feed.inet.tele.dk!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!mindspring!hydrant.mindspring.com!cssun.mathcs.emory.edu!news.service.emory.edu!news From: TJ Murphy Newsgroups: bionet.molbio.proteins.7tms_r,bionet.molbio.methds-reagnts Subject: 4823 reasons to make your own libraries Date: Wed, 27 Aug 1997 00:15:36 -0400 Organization: Emory University Lines: 63 Message-ID: <3403A9E7.8F879C1E@bimcore.emory.edu> Reply-To: medtjm@bimcore.emory.edu NNTP-Posting-Host: murphy1.pharm.emory.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.01 [en] (Win95; U) X-Priority: 3 (Normal) Xref: biosci bionet.molbio.proteins.7tms_r:1409 bionet.molbio.methds-reagnts:60701 Thought you might be interested in this warning I stumbled into on the ATCC server. By the way, reason number 4824 comes from the experience of a colleague who purchased, from one of those Southern Californian mol biol stores we get pounds of mail from every week, a human liver library that turned out to be rat. He attached a letter of apology for his wasted year from the store to his grant renewal. By the way, he got renewed. We are letting all researchers who may have received clones from the IMAGE/ Life Technologies mouse brain library or who may be interested in ordering that there is significant evidence that this library is actually from rat tissue. The following is an excerpt of an email from John Wootton (NCBI): "The initial discovery was made by my collaborators at NCI, Vadim Gladyshev and Dolph Hatfield, who, incidentally deserve credit for this and are also concerned that the community of users are made aware of the problem. They obtained human and mouse IMAGE clones matching a new gene and are carefully producing finished sequence from these clones. One clone was from the "mouse brain" library - it turned out to have 10 percent mismatches from the other mouse clones. They searched dbEST with their extended sequence from this clone and found it was identical within error to ESTs from two rat libraries. They informed me of this Tuesday July 8. I ran comprehensive searches of the dbEST sequences attributed to this library against the NCBI nr database using gapped blastn. In all cases examined, where both rat and mouse orthologs exist in nr, the ESTs from this library were identical within error to rat and significantly (typically 7-12 percent) different from mouse. The simplest explanation is that the entire library (4823 sequences in dbEST as of 7/8/97) is from rat. I did further searches to test if any other "mouse" libraries in dbEST may be rat cDNAs, using gapped blastn searches of dbEST with authentic rat sequences, including mitochondrial DNA. The only libraries in dbEST that were identical within error to these rat sequences were this "mouse brain" library and libraries labelled rat. The Life-Tech mouse embryo libraries are evidently authentic mouse. The dbEST sequences from this "mouse brain" library contain a high proportion of mitochondrial DNA matches. All of these are almost identical to the authentic rat mitochondrial sequences in GenBank. There are a few linked polymorphisms in some ESTs, which suggest that that the library is a mixture of DNA from two different strains of rat in approximate 60:40 proportion. The authentic mouse mitochondrial sequences are approximately 15 percent different from any of these rat sequences. This library also contains more than half of all the rat nuclear gene matches in dbEST: the other rat libraries are much smaller. If you want to confirm these findings, here is a sample of GenBank rat sequence accessions to use in blastn searches against dbEST: M27315, J01435, V01574 (mitochondrial DNA from different strains), M25889, J01435, M14400, J02744, X05882, X60469, X13933, M19533, M24361, L15556 (or most other rat housekeeping nuclear gene sequences)." From owner-7tms_r@net.bio.net Wed Aug 27 23:00:00 1997 Path: biosci!ERGO.COM!vboundy From: vboundy@ERGO.COM Newsgroups: bionet.molbio.proteins.7tms_r Subject: Binding with cell harvester Date: 28 Aug 1997 12:29:56 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: <199708281929.MAA25684@net.bio.net> NNTP-Posting-Host: net.bio.net Is anyone currently using a Packard FilterMate Cell Harvester (not a=20 Brandel) for receptor binding assays using an iodinated ligand? I=20 have recently joined a small biopharmaceutical company and am trying=20 to establish a receptor pharmacology lab with the existing equipment.=20= =20 I am running into quite a few stumbling blocks that neither I nor=20 Packard has been able to solve to my satisfaction. If anyone has used= =20 this system and wouldn't mind answering a few questions I'd appreciate= =20 hearing from you. Please e-mail me directly. =20 Thanks in advance. =20 -- Virginia =20 =20 Virginia A. Boundy Ergoscience, Inc. 100 First Ave., 4th floor Charlestown, MA 02129 vboundy@ergo.com From owner-7tms_r@net.bio.net Thu Aug 28 23:00:00 1997 Path: biosci!IBCUSA.COM!klangevin From: klangevin@IBCUSA.COM ("Kristen Langevin") Newsgroups: bionet.molbio.proteins.7tms_r Subject: G Protein-Coupled Receptors Date: 29 Aug 1997 09:36:54 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: <9707298728.AA872883100@ultra-gw.ibcusa.com> NNTP-Posting-Host: net.bio.net Hello, I am writing to inform you of a conference that may be of interest to you. IBC's Third Annual G Protein-Coupled Receptors conference, October 6-8, 1997 at the Loews Coronado Bay Resort in Coronado, CA. Please take a moment to view our brochure on-line at: http://www.ibcusa.com/conf/gprotein. Please contact me with any questions or to simply request a brochure. Sincerely, Kristen Langevin ============================================================ Kristen P. Langevin IBC USA Conferences, Inc. Marketing Manager Southborough Executive Park e-mail: klangevin@ibcusa.com 225 Turnpike Road TEL: (508) 481-6400 ext. 257 Southborough, MA 01772 FAX: (508) 481-4473 http://www.ibcusa.com ============================================================ From owner-7tms_r@net.bio.net Fri Aug 29 23:00:00 1997 Path: biosci!agate!hammer.uoregon.edu!vixen.cso.uiuc.edu!infeed1.internetmci.com!newsfeed.internetmci.com!192.48.96.123!in1.uu.net!195.31.190.112!news.tin.it!news From: Roberto Newsgroups: bionet.molbio.proteins.7tms_r Subject: not only cars Date: 30 Aug 1997 14:01:12 GMT Organization: Dreamcars Lines: 8 Message-ID: <5u9938$3or@everest.vol.it> NNTP-Posting-Host: 195.103.98.200 we have dreamcars even your one. Let us know what you are looking for, we will have it. send e-mail to dreamcars@webko.it