From owner-7tms_r@net.bio.net Thu Oct 02 23:00:00 1997 Path: biosci!biosci!not-for-mail From: RABond@UH.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: Post-Doctoral position Date: 3 Oct 1997 03:32:46 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <612hke$dt3@net.bio.net> NNTP-Posting-Host: net.bio.net POST-DOCTORAL POSITION AVAILABLE: The primary interest of the lab is receptor theory for GPCRs using the Beta2-adrenoceptor as the prototype. We have been primarily performing whole animal and isolated tissue studies from transgenic and knockout mice in collaboration with Bob Lefkowitz. We would like to expand into transfected cell systems and are seeking people with experience in transfection of receptors, radioligand binding assays and second messenger assays. The position is available for 2 or 3 years. Please contact: Richard A. Bond, Ph.D. University of Houston 4800 Calhoun Houston, TX 77204-5515 phone: (713) 743-1210 or (713) 743-1219 fax: (713) 743-1229 e-mail: RABond@UH.Edu Richard A. Bond, Ph.D.: Department of Pharmacological and Pharmaceutical Sciences, University of Houston, 4800 Calhoun, Houston, Texas 77204-5515. E-mail: RABond@UH.edu telephones: office: (713) 743-1210, lab: (713) 743-1219 fax: (713) 743-1229. From owner-7tms_r@net.bio.net Thu Oct 02 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Gennady PODA Newsgroups: bionet.molbio.proteins.7tms_r Subject: 7tm_r: chemokine receptor dimerization summary Date: 3 Oct 1997 03:33:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 113 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <612hlo$dtr@net.bio.net> NNTP-Posting-Host: net.bio.net Dear Netters, I have nothing to add about this problem. All the answers I got were people did not come across any experimental confirmation about the dimerization of the chemokine receptors. Since the topic is quite hot so I could conclude that the people who are doing now such experiments mostly keep silence unless the results got published. So we have to wait a bit. Thanks a lot for everybody who answered. With best wishes, Gennady The answers are here: From: Vinnie Terranova Subject: Re: Dimerization of chemokine receptors Actually, I am setting up to examine exactly this phenomenon. As soon as I get some results, I could e-mail you. On Tue, 30 Sep 1997, Gennady PODA wrote: > > Dear experimentalists, > > previously the phemonenon of dimerization of GPCRs has been discussed in > short in this newsgroup. Now I am wondering about a more particular question. > > Has any one seen (done) an experimental confirmation that chemokine > receptors (any of them) can (or can not) form dimers? I could not find in > the literature any of such references. I will summarize the answers. > Thanks a lot in advance for participation. > > With best wishes, > > Gennady > > > > > ******************************************************************* > * Dr Gennady Poda * > ******************************************************************* > * Laboratoire de Chimie theorique (UA 510 au CNRS) * > * Universite Henri Poincare, Nancy-I * > * Faculte des Sciences - Domaine scientifique Victor Grignard * > * BP 239 - 54506 Vandoeuvre-les-Nancy Cedex France * > * * > * Tel (lab) + 33 (0)3.83.91.20.50 E-mail poda@incm.u-nancy.fr * > * FAX + 33 (0)3.83.91.25.30 gpoda@loria.fr * > ******************************************************************* > From: Stanley Krystek Subject: chemokine receptors I have not seen anyone modeling the dimerization of these receptors and would be interested in any responses that you get concerning experimental evidence. thanks stan krystek Stanley R. Krystek, Jr., Ph.D. Bristol-Myers Squibb Pharmaceutical Research Institute Department of Macromolecular Modeling P.O. Box 4000, Room H.3812, Princeton, N.J. 08543-4000 (609) 252-4100 FAX: (609) 252-6030 INTERNET: krystek@bms.com From: hoebeke@univ-tours.fr Subject: Re: Dimerization of chemokine receptors Dear Gennady, As far as I know, nobody as yet gas discussed dimerisation of chemokine receptors. Yours, Johan Hoebeke ******************************************************************* * Dr Gennady Poda * ******************************************************************* * Laboratoire de Chimie theorique (UA 510 au CNRS) * * Universite Henri Poincare, Nancy-I * * Faculte des Sciences - Domaine scientifique Victor Grignard * * BP 239 - 54506 Vandoeuvre-les-Nancy Cedex France * * * * Tel (lab) + 33 (0)3.83.91.20.50 E-mail poda@incm.u-nancy.fr * * FAX + 33 (0)3.83.91.25.30 gpoda@loria.fr * ******************************************************************* From owner-7tms_r@net.bio.net Thu Oct 02 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Gert.Vriend@EMBL-Heidelberg.de Newsgroups: bionet.molbio.proteins.7tms_r Subject: which cells express which receptors? Date: 3 Oct 1997 10:40:39 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <613amn$5k3@net.bio.net> NNTP-Posting-Host: net.bio.net Dear GPCR friends, There are regularly questions in this news group regarding receptors endogenously expressed in certain cell-lines. We discussed this topic earlier this year and many people sent data to Agi Schonbrunn who made a good WWW based list. The pointer to that data is: http://www.biomedcomp.com/GPCR.html (also reachable from the "pointers to GPCR sites" list on http://www.gpcr.org) Perhaps everybody should take a look if they have data available to them that is not yet included in this list (the last update was june 9-th, and some discoveries must have been made since that time....). Greetings Gert Vriend From owner-7tms_r@net.bio.net Thu Oct 02 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Steve Alexander Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: adrenoreceptors Date: 3 Oct 1997 10:41:28 -0700 Organization: University of Nottingham Lines: 46 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <613ao8$5p1@net.bio.net> References: <612k4r$ipr@net.bio.net> Reply-To: steve.alexander@nottingham.ac.uk.nospam NNTP-Posting-Host: net.bio.net BALWINDER SAMBI wrote: > > would appreciate any information on cells which endogenously express > either the alpha 2 or beta 2adrenoreceptor From my database records, alpha2 's are endogenously expressed in: PC12 rat phaeochromocytoma (Gatti et al. (1988) BiochemJ 255,753-760) HT 29 (Jones and Bylund. (1988)JBiolChem 263,14236-14244) NG108-15 murine neuroblastoma rat glioma hybrid (Louie et al. (1990)JPharmacolExpTher 253,401-407; Strassheim and Malbon. (1994)JBiolChem 269,14307-14313) SH-SY5Y human neuroblastoma (Lameh et al. (1992)EurJPharmacol-MolPharmacol 227,19-24) SK-N-BE human neuroblastoma (Namir et al. (1997)JNeurochem 68,1764-1772) NAL-GT human neuroblastoma (Lambert. (1995)NeurosciLett 183,4-7) beta2 's are endogenously expressed in: C6 rat glioma (Ruck et al. (1990)BiochemPharmacol 40,2371-2375; Hosoda et al. (1995)MolPharmacol 48,206-211; Zhong and Minneman. (1995)JPharmacolExpTher 272,1088-1094; McCrea and Hill. (1996)MolPharmacol 49,927-937) B50 mouse neuroblastoma (Ruck et al. (1990)BiochemPharmacol 40,2371-2375; McCrea and Hill. (1993)BrJPharmacol 110,619-626) U937 human promonocytic (Pauleugene et al. (1993)MolecularImmunology 30,157-164) S49 mouse lymphoma (Clark et al. (1993)JBiolChem 268,3245-3250; Post et al. (1996)JBiolChem 271,895-900) I'm sure that there must be others, though. - -- ::::::::::::::::::::::::::::::::::::::::::::::: Steve Alexander, Biomedical Sciences, Nottingham NG7 2UH, UK WWW http://www.nottingham.ac.uk/biomedsci/ %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% From owner-7tms_r@net.bio.net Thu Oct 02 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Gennady PODA Newsgroups: bionet.molbio.proteins.7tms_r Subject: 7tm_r: chemokine receptor dimerization summary Date: 3 Oct 1997 03:39:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 113 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <612i06$ehm@net.bio.net> NNTP-Posting-Host: net.bio.net Dear Netters, I have nothing to add about this problem. All the answers I got were people did not come across any experimental confirmation about the dimerization of the chemokine receptors. Since the topic is quite hot so I could conclude that the people who are doing now such experiments mostly keep silence unless the results got published. So we have to wait a bit. Thanks a lot for everybody who answered. With best wishes, Gennady The answers are here: From: Vinnie Terranova Subject: Re: Dimerization of chemokine receptors Actually, I am setting up to examine exactly this phenomenon. As soon as I get some results, I could e-mail you. On Tue, 30 Sep 1997, Gennady PODA wrote: > > Dear experimentalists, > > previously the phemonenon of dimerization of GPCRs has been discussed in > short in this newsgroup. Now I am wondering about a more particular question. > > Has any one seen (done) an experimental confirmation that chemokine > receptors (any of them) can (or can not) form dimers? I could not find in > the literature any of such references. I will summarize the answers. > Thanks a lot in advance for participation. > > With best wishes, > > Gennady > > > > > ******************************************************************* > * Dr Gennady Poda * > ******************************************************************* > * Laboratoire de Chimie theorique (UA 510 au CNRS) * > * Universite Henri Poincare, Nancy-I * > * Faculte des Sciences - Domaine scientifique Victor Grignard * > * BP 239 - 54506 Vandoeuvre-les-Nancy Cedex France * > * * > * Tel (lab) + 33 (0)3.83.91.20.50 E-mail poda@incm.u-nancy.fr * > * FAX + 33 (0)3.83.91.25.30 gpoda@loria.fr * > ******************************************************************* > From: Stanley Krystek Subject: chemokine receptors I have not seen anyone modeling the dimerization of these receptors and would be interested in any responses that you get concerning experimental evidence. thanks stan krystek Stanley R. Krystek, Jr., Ph.D. Bristol-Myers Squibb Pharmaceutical Research Institute Department of Macromolecular Modeling P.O. Box 4000, Room H.3812, Princeton, N.J. 08543-4000 (609) 252-4100 FAX: (609) 252-6030 INTERNET: krystek@bms.com From: hoebeke@univ-tours.fr Subject: Re: Dimerization of chemokine receptors Dear Gennady, As far as I know, nobody as yet gas discussed dimerisation of chemokine receptors. Yours, Johan Hoebeke ******************************************************************* * Dr Gennady Poda * ******************************************************************* * Laboratoire de Chimie theorique (UA 510 au CNRS) * * Universite Henri Poincare, Nancy-I * * Faculte des Sciences - Domaine scientifique Victor Grignard * * BP 239 - 54506 Vandoeuvre-les-Nancy Cedex France * * * * Tel (lab) + 33 (0)3.83.91.20.50 E-mail poda@incm.u-nancy.fr * * FAX + 33 (0)3.83.91.25.30 gpoda@loria.fr * ******************************************************************* From owner-7tms_r@net.bio.net Thu Oct 02 23:00:00 1997 Path: biosci!biosci!not-for-mail From: BALWINDER SAMBI Newsgroups: bionet.molbio.proteins.7tms_r Subject: adrenoreceptors Date: 3 Oct 1997 04:15:39 -0700 Organization: University of Paisley Lines: 2 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <612k4r$ipr@net.bio.net> NNTP-Posting-Host: net.bio.net would appreciate any information on cells which endogenously express either the alpha 2 or beta 2adrenoreceptor From owner-7tms_r@net.bio.net Sun Oct 05 23:00:00 1997 Path: biosci!biosci!not-for-mail From: CES@novo.dk (CES) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Direct photoaffinity labelling Date: 6 Oct 1997 06:04:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <61anl3$bu9@net.bio.net> NNTP-Posting-Host: net.bio.net Dear 7tm netters. Does anyone have a protocol for UV mediated photoaffinity labelling by a 125I-labelled ligand of a target protein? The ligand in question has no photo-reactive groups like azido, but just contains a (photo-labile?) alkyliodide. Please reply to ces@novo.dk Carsten E. Stidsen Molecular Pharmacology Novo Nordisk A/S Denmark From owner-7tms_r@net.bio.net Mon Oct 06 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "A. Schonbrunn" Newsgroups: bionet.molbio.proteins.7tms_r Subject: endogenous receptors Date: 7 Oct 1997 14:36:04 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <61ea04$8hj@net.bio.net> NNTP-Posting-Host: net.bio.net Dear all: I have just updated the endogenous receptor list posted at: http://www.biomedcomp.com/GPCR.html If you have a favorite cell line that you would like to have included and, better yet, can e-mail me information of which receptors are expressed, I will continue to add to the list. Thanks for the input from those who wrote previously. Agi Schonbrunn From owner-7tms_r@net.bio.net Sat Oct 11 23:00:00 1997 Path: biosci!biosci!not-for-mail From: daemon@net.bio.net Newsgroups: bionet.molbio.proteins.7tms_r Subject: BioScience Information Date: 12 Oct 1997 09:30:44 -0700 Organization: The Science Guide Lines: 90 Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <61qtvk$3d9@net.bio.net> Reply-To: news@scienceguide.com NNTP-Posting-Host: net.bio.net Keywords: Science Directory, Conference Info, Science News, Medical I= Announcing the SCIENCE GUIDE. http://www.scienceguide.com A New Internet Directory and Information Service run by Scientists an= d Physicians for Scientists and Physicians. After visiting the Guide, = If you have any suggestion for making the Guide better please let us kno= w. (webmaster@scienceguide.com) The Science Guide consists of a number of different sections designed= to help the scientist and physician find information on the internet and= to sponsor communication between those interested in science: NEWS SECTION Every day the Science Guide compiles medical and research news from national news sources around the net. Most of the news articles are concerned with medicine, bioscience, and physics, but all other scien= ces =66rom agriculture to zoology are commonly included. News sources cur= rently listed include: CNN, EurekAlert, HMS Beagle, MSNBC Sci-Tech, Science Magazine=B9s ScienceNow, CBS Space News, USA Today, The Albuquerque J= ournal, Scientific American Web Weekly, The Why Files, Discover Magazine, Scientific American, Smithsonian Magazine, and the Technology Review.= The news pages also list links to news sources not compiled within the Ne= ws site. We are currently working on adding a number of other sources t= o the site to make it even more useful. To make getting science news even easier, we send out a DAILY NEWS EM= AILER listing the articles which have been compiled on our site. Anyone ca= n subscribe to the Emailer by sending an email to news@scienceguide.com= with the message =B3Subscribe=B2 DIRECTORY OF USENET NEWS GROUPS and DISCUSSION LISTS The Directory of Usenet and Discussion Groups is compiled quarterly f= rom different sources around the net to provide the scientist and those interested in science easy access to these invaluable sources of disc= ourse and information. We are currently working on finding the proper subscription method for each of the discussion lists. This is taking= a bit longer that we thought so please pardon our dust. The Usenet por= tions of this section are complete. ON-LINE JOURNAL HYPERLINK SECTION The Journals Section contains links to peer reviewed scientific journ= als on the Internet. Each listing clearly indicates whether the journal provides only the table of contents, TOC with abstracts, or the full = text of the journal EMPLOYMENT SECTION The Jobs and Positions Section contains hyperlinks to the best Scient= ific Employment Databases and Classifieds on the net. GRANTS and FUNDING SECTION The funding section contains links to the best funding and grant data= bases on the Internet, making it very easy for scientists to quickly find funding opportunities. The featured site of the section is =B3The Co= mmunity of Science,=B2 a Johns Hopkins service designed to help scientists fi= nd and continue funding. From owner-7tms_r@net.bio.net Sat Oct 11 23:00:00 1997 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.molbio.proteins.7tms_r Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 12 Oct 1997 09:30:58 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 232 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <61qu02$3eb@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! - ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. - -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. - ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? - --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? - ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. - ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI - ------------------------------------------------------------------ node at computer net.bio.net: - ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at - -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): - ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. - ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-7tms_r@net.bio.net Wed Oct 15 23:00:00 1997 Path: biosci!biosci!not-for-mail From: steph angers Newsgroups: bionet.molbio.proteins.7tms_r Subject: Endogenous cAMP Levels Date: 16 Oct 1997 15:22:23 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <62642v$4lq@net.bio.net> NNTP-Posting-Host: net.bio.net HI, I was wondering if anyone knew the different endogeneous cAMP levels for different cell lines, like CHO, COS, HELA, BHK, 293..... Thanks, Stephane Angers Biosignal Inc. research@biosignal.com ------- End of forwarded message ------- From owner-7tms_r@net.bio.net Thu Oct 16 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Bruno Cenni Newsgroups: bionet.molbio.proteins.7tms_r Subject: D1 receptor antibody Date: 17 Oct 1997 06:06:30 -0700 Organization: University of Geneva Lines: 23 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <627nsm$3q5@net.bio.net> NNTP-Posting-Host: net.bio.net Hi all, does anyone know a source for an antibody against human dopamine D1 receptors, possibly one that works in immunihistochemistry? We are trying to screen cell lines for presence or absence of D1DR. Thanks Bruno ******************************************************************* Bruno Cenni Ph.D. University of Geneva Dept. of Cell Biology Sciences III 30 Quai Ernest Ansermet 1211 Geneva 4 Switzerland Phone: ++41 22 702 6811 Fax: ++41 22 702 6442 cenni@cellbio.unige.ch From owner-7tms_r@net.bio.net Thu Oct 16 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Michael Levine Newsgroups: bionet.molbio.proteins.7tms_r Subject: somatostatin receptor Date: 17 Oct 1997 12:39:00 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 4 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <628esk$mns@net.bio.net> NNTP-Posting-Host: net.bio.net Does anyone have a good antiserum for the type 2 somatostatin receptor that can be used for immunocytochemistry? Michael Levine From owner-7tms_r@net.bio.net Fri Oct 17 23:00:00 1997 Path: biosci!biosci!not-for-mail From: newsmgr@merrimack.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Gq and Bkt? Date: 18 Oct 1997 14:09:33 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <62b8id$t7n@net.bio.net> NNTP-Posting-Host: net.bio.net Relay-Version: ANU News - V6.2.0 06/23/97 OpenVMS AXP V6.2; site chasm Path: chasm!cam-news-feed2.bbnplanet.com!cam-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!mindspring!news.mindspring.com!usenet Newsgroups: bionet.molbio.proteins.7tms_r Subject: Gq and Bkt? Message-ID: <3448E003.AEAE3BD0@emory.edu> From: Rick Bright Date: Sat, 18 Oct 1997 12:12:51 -0400 Organization: Emory University, Molecular Therapeutics & Toxicology Lines: 5 NNTP-Posting-Host: user-38lcamc.dialup.mindspring.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Server-Date: 18 Oct 1997 16:13:55 GMT X-Mailer: Mozilla 4.03 [en] (Win95; U) Does anyone know of the relation between Gq and Bkt kinase? I would appreciate any references. Thanks From owner-7tms_r@net.bio.net Fri Oct 24 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Christophe GUENIN" Newsgroups: bionet.molbio.proteins.7tms_r Subject: Cellular adherence Date: 25 Oct 1997 11:28:01 -0700 Organization: None Lines: 5 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <62tdnh$bai@net.bio.net> NNTP-Posting-Host: net.bio.net I have a cellular adherence problem when I am doing fluorescence in glass slide with polylysine with transfected cell cos7. My cellular cover is damaged when i'm washing it and /or antibody marquage. You can reply in this news group or at the following e mail : christophe.guenin@hol.fr From owner-7tms_r@net.bio.net Sat Oct 25 23:00:00 1997 Path: biosci!biosci!not-for-mail From: daemon@net.bio.net Newsgroups: bionet.molbio.proteins.7tms_r Subject: Updated WWWsite: The Antibody Resource Page Date: 26 Oct 1997 14:51:24 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 61 Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <630hhc$chg@net.bio.net> NNTP-Posting-Host: net.bio.net Relay-Version: ANU News - V6.2.0 06/23/97 OpenVMS AXP V6.2; site chas= m Path: chasm!cam-news-feed2.bbnplanet.com!cam-news-hub1.bbnplanet.com!= news.bbnplanet.com!news.maxwell.syr.edu!newsfeed.internetmci.com!131.= 103.1.114!iagnet.net!nntp.earthlink.net!usenet Newsgroups: bionet.molbio.proteins.7tms_r Subject: Updated WWWsite: The Antibody Resource Page Message-ID: <3453464A.12C0@znet.com> =46rom: kshreder@znet.com Date: Sun, 26 Oct 1997 05:31:54 -0800 Reply-To: kshreder@znet.com Organization: The Antibody Resource Page Lines: 34 NNTP-Posting-Host: 153.35.100.12 Mime-Version: 1.0 Content-Type: text/plain; charset=3Diso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 3.0Gold (Win16; U) Updated WWWsite: The Antibody Resource Page The Antibody Resource Page has been recently updated. The page will = be invaluable to researchers and educators alike. Here is just some of what can be found on the page: 1. How to Find an Antibody - a variety of ways on and off the web to find the antibody you are looking for. 2. Online Companies - links to over 110 companies that sell antibodie= s or antibody related products. Is your company listed on this page? 3. Antibody Image Gallery - some animated gifs have recently been add= ed 4. Bulletin Board - Have a question or have an answer? Then stop by = and post a message. 5. Educational Resources - a variety of new links have been added.= =20 There are links to pages on immunochemistry, antibody production,= =20 autoimmunity, vaccines, immunology and much more. This page is divid= ed up into sections on research, educational, and health resources. 6. The Latest in Antibody News - Get up-to-date, antibody-related articles on topics from academia and industry. =2E..and there is much more. Check it out at: http://www.antibodyresource.com/ Ps. Don=92t forget to visit our sponsors, Research Diagnostics, Inc. (http://www.researchd.com/absort1.htm) and Lab Vision Corporation (http://www.labvision.com/)!