From bogus@does.not.exist.com Sun Jun 12 19:41:10 2005 From: bogus@does.not.exist.com () Date: Sun Jun 12 19:41:11 2005 Subject: No subject Message-ID: Genomics 1995 Sep 20;29(2):329-334 Analysis of the 5' region of PMS2 reveals heterogeneous transcripts and a novel overlapping gene. Nicolaides NC, Kinzler KW, Vogelstein B Howard Hughes Medical Institute, Baltimore, Maryland 21231, USA. The PMS2 gene encodes a protein that is involved in DNA mismatch repair and is mutated in a subset of patients with hereditary nonpolyposis colon cancer (HNPCC). The previously published PMS2 cDNA sequence lacks an upstream in-frame stop codon preceding the presumptive initiating methionine. To evaluate the 5' terminus of the PMS2 coding region further, we isolated additional cDNA clones, RT-PCR products, and the corresponding 5' genomic segment of the PMS2 locus. The PMS2 gene transcripts were found to have heterogeneous but colinear 5' termini, one of which contained an in-frame termination codon preceding the initiating methionine. In addition, a novel gene encoding a 34.5-kDa polypeptide was found to initiate transcriptionally within PMS2 from the opposite strand. -- Chris Boyd | from, | MRC Human Genetics Unit chrisb@hgu.mrc.ac.uk | not | Western General Hospital http://www.hgu.mrc.ac.uk/~chrisb | for | Edinburgh EH4 2XU, SCOTLAND From biosci at net.bio.net Fri Jun 17 09:51:07 2005 From: biosci at net.bio.net (BIOSCI Administrator) Date: Thu Jun 23 15:03:44 2005 Subject: [Genstructure] BIOSCI/Bionet has a new home Message-ID: <200506171451.j5HEp7X00288@net.bio.net> Dear GENSTRUCTURE readers, BIOSCI/Bionet is the long-running Biology news and discussion groups. The management of these groups has been handled ably at MRC/Rosalind Franklin Centre for several years now. They are turning over management to me, Don Gilbert, at Indiana University Biology department. The MRC/RFC folks deserve our thanks for their devotion to maintaining this useful and unique biology news service. Web access continues at http://www.bio.net/ Find there GENSTRUCTURE links to Read/Subscribe/Post messages. E-mail postings continue to be through genstruc @ net.bio.net The new home is at IUBio Archive, iubio.bio.indiana.edu, which I've maintained for over 15 years. The names net.bio.net and www.bio.net, and their related BIOSCI roles continue to work. Please keep using these bio.net addresses, they will continue when the host computer changes. There may be some hiccups over the coming weeks as the new BIOSCI home gets a work out. Please bear with us, and let us know if problems persist longer. Regards, Don Gilbert Biology Dept., Indiana University Bloomington, Indiana, USA 47405 BIOSCI help mail: biosci-help @ net.bio.net ----------------------------------------------